Cytokinetic diversity in mammalian cells is revealed by the characterization of endogenous anillin, Ect2 and RhoA.
Husser MC, Ozugergin I, Resta T, Martin VJJ, Piekny AJ
Open Biol. 2022 Nov;12(11):220247. doi: 10.1098/rsob.220247. Epub 2022 Nov 23.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 183834 | pODN-mNG-ANLN | Repair template for the N-terminal tagging of anillin with mNeonGreen in human cells using CRISPR/Cas9. |
| 183835 | pODN-mNG-ECT2 | Repair template for the N-terminal tagging of Ect2 with mNeonGreen in human cells using CRISPR/Cas9. |
| 183836 | pODN-mNG-RHOA | Repair template for the N-terminal tagging of RhoA with mNeonGreen in human cells using CRISPR/Cas9. |
| 183837 | pODN-mNG-CfANLN | Repair template for the N-terminal tagging of anillin with mNeonGreen in canine cells using CRISPR/Cas9. |
| 183866 | pODN-H2BC11-mR2 | Repair template for the C-terminal tagging of H2B histones with mRuby2 in human cells using CRISPR/Cas9. |
| 183867 | pODN-H2BC11-TagBFP | Repair template for the C-terminal tagging of H2B histones with mTagBFP in human cells using CRISPR/Cas9. |
| 183868 | pODN-mR2-ACTB | Repair template for the N-terminal tagging of beta-actin with mRuby2 in human cells using CRISPR/Cas9. |
| 183869 | pODN-mR2-MYH10 | Repair template for the N-terminal tagging of myosin heavy chain 10 with mRuby2 in human cells using CRISPR/Cas9. |
| 183870 | pODN-AAVS1-mNG-CAAX | Repair template for the stable expression of a mNeonGreen-CAAX fusion in human cells using CRISPR/Cas9. |
| 183871 | pODN-AAVS1-mR2-CAAX | Repair template for the stable expression of a mRuby2-CAAX fusion in human cells using CRISPR/Cas9. |
| 183872 | pX459V2.0-HypaCas9-mRuby2 | pX459V2.0 plasmid expressing HypaCas9 with P2A-mRuby2 and T2A-Puro, with a sgRNA cloning site for CRISPR editing in mammalian cells. |
| 183873 | pX459-HypaCas9-ECT2_sgRNA | pX459V2.0-HypaCas9 plasmid with ECT2 sgRNA for N-terminal tagging of Ect2 in human cells. |
| 183874 | pX459-HypaCas9-ANLN_sgRNA | pX459V2.0-HypaCas9 plasmid with ANLN sgRNA for N-terminal tagging of anillin in human cells. |
| 183875 | pX459-HypaCas9-mR2-ECT2_sgRNA | pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and ECT2 sgRNA for N-terminal tagging of Ect2 in human cells. |
| 183876 | pX459-HypaCas9-mR2-ANLN_sgRNA | pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and ANLN sgRNA for N-terminal tagging of anillin in human cells. |
| 183877 | pX459-HypaCas9-mR2-RHOA_sgRNA | pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and RHOA sgRNA for N-terminal tagging of RhoA in human cells. |
| 183878 | pX459-HypaCas9-RHOA_sgRNA | pX459V2.0-HypaCas9 plasmid with RHOA sgRNA for N-terminal tagging of RhoA in human cells. |
| 183879 | pX459-HypaCas9-mR2-CfANLN_sgRNA | pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and cfANLN sgRNA for N-terminal tagging of anillin in canine (Canis familiaris) cells. |
| 183880 | pX459-HypaCas9-CfANLN_sgRNA | pX459V2.0-HypaCas9 plasmid with cfANLN sgRNA for N-terminal tagging of anillin in canine (Canis familiaris) cells. |
| 183881 | pX459-HypaCas9-mR2-H2BC11_sgRNA | pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and H2BC11 sgRNA for C-terminal tagging of H2B histones in human cells. |
| 183883 | pX459-HypaCas9-H2BC11_sgRNA | pX459V2.0-HypaCas9 plasmid with H2BC11 sgRNA for C-terminal tagging of H2B histones in human cells. |
| 183884 | pX459-HypaCas9-mR2-ACTB_sgRNA | pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and ACTB sgRNA for N-terminal tagging of beta-actin in human cells. |
| 183885 | pX459-HypaCas9-ACTB_sgRNA | pX459V2.0-HypaCas9 plasmid with ACTB sgRNA for N-terminal tagging of beta-actin in human cells. |
| 183886 | pX459-HypaCas9-mR2-MYH10_sgRNA | pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and MYH10 sgRNA for N-terminal tagging of myosin heavy chain 10 in human cells. |
| 183887 | pX459-HypaCas9-MYH10_sgRNA | pX459V2.0-HypaCas9 plasmid with MYH10 sgRNA for N-terminal tagging of myosin heavy chain 10 in human cells. |
| 183888 | pX459-HypaCas9-mR2-TUBA1B_sgRNA | pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and TUBA1B sgRNA for N-terminal tagging of alpha-tubulin in human cells. |
| 183889 | pX459-HypaCas9-TUBA1B_sgRNA | pX459V2.0-HypaCas9 plasmid with TUBA1B sgRNA for N-terminal tagging of alpha-tubulin in human cells. |
| 183890 | pX459-HypaCas9-mR2-AAVS1_sgRNA | pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and sgRNA targeting the AAVS1 in human cells. |
| 183891 | pX459-HypaCas9-AAVS1_sgRNA | pX459V2.0-HypaCas9 plasmid with sgRNA targeting the AAVS1 in human cells. |