Direct fluorescent labeling of NF186 and NaV1.6 in living primary neurons using bioorthogonal click chemistry.
Stajkovic N, Liu Y, Arsic A, Meng N, Lyu H, Zhang N, Grimm D, Lerche H, Nikic-Spiegel I
J Cell Sci. 2023 Jun 15;136(12):jcs260600. doi: 10.1242/jcs.260600. Epub 2023 Jun 28.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 239762 | pcDNA3.1(+)_Nav1.6WT-HA | Expresses mouse Nav1.6WT with a C-terminal HA tag in mammalian cells. |
| 239763 | pcDNA3.1(+)_Nav1.6(K1425TAG)-HA | Expresses mouse Nav1.6 with a TAG codon at position 1425 and a C-terminal HA tag in mammalian cells. Can be used for click labeling. |
| 239764 | pcDNA3.1(+)_Nav1.6(K1546TAG)-HA | Expresses mouse Nav1.6 with a TAG codon at position 1546 and a C-terminal HA tag in mammalian cells. Can be used for click labeling. |
| 239765 | pcDNA3.1(+)_Nav1.6(WT, Y371C)-HA | Expresses TTX-resistant (Y371C) variant of mouse Nav1.6WT with a C-terminal HA tag in mammalian cells. |
| 239766 | pcDNA3.1(+)_Nav1.6(K1425TAG, Y371C)-HA | Expresses TTX-resistant (Y371C) variant of mouse Nav1.6 with a TAG codon at position 1425 and a C-terminal HA tag in mammalian cells. Can be used for click labeling. |
| 239767 | pcDNA3.1(+)_Nav1.6(K1546TAG, Y371C)-HA | Expresses TTX-resistant (Y371C) variant of mouse Nav1.6 with a TAG codon at position 1546 and a C-terminal HA tag in mammalian cells. Can be used for click labeling. |
| 239768 | pcDNA3.1(+)_Nav1.6(WT, Y371C)-P2A-eGFP | Expresses independantly TTX-resistant (Y371C) variant of mouse Nav1.6WT and eGFP in mammalian cells. |
| 239769 | pcDNA3.1(+)_Nav1.6(K1425TAG, Y371C)-P2A-eGFP | Expresses independantly TTX-resistant (Y371C) variant of mouse Nav1.6 with a TAG codon at position 1425 and eGFP in mammalian cells. |
| 239770 | pcDNA3.1(+)_Nav1.6(K1425TAG, Y371C, I1652N)-HA | Expresses pathogenic (I1652N) TTX-resistant variant of mouse Nav1.6 with a TAG codon at position 1425 and a C-terminal HA tag in mammalian cells. Can be used for click labeling. |
| 239771 | pcDNA3.1(+)_Nav1.6(K1546TAG, Y371C , I1652N)-HA | Expresses pathogenic (I1652N) TTX-resistant variant of mouse Nav1.6 with a TAG codon at position 1546 and a C-terminal HA tag in mammalian cells. Can be used for click labeling. |
| 239772 | pcDNA3.1(+)_Nav1.6(K1425TAG, Y371C, T1785P)-HA | Expresses pathogenic (T1785P) TTX-resistant variant of mouse Nav1.6 with a TAG codon at position 1425 and a C-terminal HA tag in mammalian cells. Can be used for click labeling. |
| 239773 | pcDNA3.1(+)_Nav1.6(K1546TAG, Y371C , T1785P)-HA | Expresses pathogenic (T1785P) TTX-resistant variant of mouse Nav1.6 with a TAG codon at position 1546 and a C-terminal HA tag in mammalian cells. Can be used for click labeling. |
| 239774 | CMV-NF186WT-HA | Expresses WT rat NF186 with a C-terminal HA tag in mammalian cells. |
| 239775 | CMV-NF186(K519TAG)-HA | Expresses rat NF186 with a TAG codon at position 519 and a C-terminal HA tag in mammalian cells. Can be used for click labeling. |
| 239776 | CMV-NF186(K534TAG)-HA | Expresses rat NF186 with a TAG codon at position 534 and a C-terminal HA tag in mammalian cells. Can be used for click labeling. |
| 239777 | CMV-NF186(K571TAG)-HA | Expresses rat NF186 with a TAG codon at position 571 and a C-terminal HA tag in mammalian cells. Can be used for click labeling. |
| 239778 | CMV-NF186(K604TAG)-HA | Expresses rat NF186 with a TAG codon at position 604 and a C-terminal HA tag in mammalian cells. Can be used for click labeling. |
| 239779 | CMV-NF186(K680TAG)-HA | Expresses rat NF186 with a TAG codon at position 680 and a C-terminal HA tag in mammalian cells. Can be used for click labeling. |
| 239780 | CMV-NF186(K809TAG)-HA | Expresses rat NF186 with a TAG codon at position 809 and a C-terminal HA tag in mammalian cells. Can be used for click labeling. |
| 239781 | hNSE-NF186WT-HA | Expresses WT rat NF186 and with a C-terminal HA tag in mammalian cells. |
| 239782 | hNSE-NF186(K519TAG)-HA | Expresses rat NF186 with a TAG codon at position 519 and a C-terminal HA tag in mammalian cells. Can be used for click labeling. |
| 239783 | hNSE-NF186(K534TAG)-HA | Expresses rat NF186 with a TAG codon at position 534 and a C-terminal HA tag in mammalian cells. Can be used for click labeling. |
| 239784 | hNSE-NF186(K571TAG)-HA | Expresses rat NF186 with a TAG codon at position 571 and a C-terminal HA tag in mammalian cells. Can be used for click labeling. |
| 239785 | hNSE-NF186(K604TAG)-HA | Expresses rat NF186 with a TAG codon at position 604 and a C-terminal HA tag in mammalian cells. Can be used for click labeling. |
| 239786 | hNSE-NF186(K680TAG)-HA | Expresses rat NF186 with a TAG codon at position 680 and a C-terminal HA tag in mammalian cells. Can be used for click labeling. |
| 239787 | hNSE-NF186(K809TAG)-HA | Expresses rat NF186 with a TAG codon at position 809 and a C-terminal HA tag in mammalian cells. Can be used for click labeling. |