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SARS-CoV-2 3CL(pro) (main protease) regulates caspase activation of gasdermin-D/E pores leading to secretion and extracellular activity of 3CL(pro).

Grin PM, Baid K, de Jesus HCR, Kozarac N, Bell PA, Jiang SZ, Kappelhoff R, Butler GS, Leborgne NGF, Pan C, Pablos I, Machado Y, Vederas JC, Kim H, Benarafa C, Banerjee A, Overall CM
Cell Rep. 2024 Dec 24;43(12):115080. doi: 10.1016/j.celrep.2024.115080. Epub 2024 Dec 12. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
218229pcDNA3.1-GSDMD_1-270Gasdermin1-270 is a pore-forming GSDMD formed by SARS-CoV-2 3CLpro cleavage. Transfection in HEK293 cells showed GSDMD 1-270 pores secrete 3CLpro, nucleocapsid, and in excess, kill cells by pyroptosis
218230pcDNA3.1-GSDMD_1-275Gasdermin-D_1-275 is a pore-forming GSDMD formed by cleavage by host caspases. Transfection in HEK293 cells showed GSDMD 1-275 pores secrete 3CLpro, nucleocapsid; in excess, kills cells by pyroptosis
218231pcDNA3.1-GSDMD_29-484Gasdermin-D_29-484 is a non-functional GSDMD formed by SARS-CoV-2 3CLpro cleavage in the N-terminal domain. Transfection in HEK293 cells showed no pore-forming activity or induction of pyroptosis
218232pcDNA3.1-GSDMD_29-270Gasdermin-D_29-270 is a non-functional GSDMD formed by two SARS-CoV-2 3CLpro cleavages. Transfection in HEK293 cells showed no pore-forming activity or induction of pyroptosis
224697pET21b(+)_SARS-CoV-2_3CLpro_Q306A-HISBacterial Expression and Purification of SARS-CoV-2 3CLpro active protease with His-tag
224698pET21b(+)_SARS-CoV-2_3CLpro_C145A-HISBacterial Expression and Purification of SARS-CoV-2 3CLpro inactive protease with His-tag
224701pcDNA3.1-GSDMD_H270AExpression of mutated Gasdermin D_H270A (GSDMD_H270A) in transfected mammalian cell lines
224702pcDNA3.1-GSDMD_Q29AExpression of mutated Gasdermin D_Q29A (GSDMD_Q29A) in transfected mammalian cell lines
224703pcDNA3.1-GSDMD_Q29A_H270AExpression of mutant Gasdermin D_Q29A_H270A (GSDMD_Q29A_H270A) in transfected mammalian cell lines
224704pcDNA3.1-GSDMD_D275AExpression of mutated Gasdermin D_D275A (GSDMD_D275A) in transfected mammalian cell lines
224705pcDNA3.1-GSDMD_H270A_D275AExpression of mutated Gasdermin D_H270A_D275A (GSDMD_D275A) in transfected mammalian cell lines

Antibodies from Article