SARS-CoV-2 3CL(pro) (main protease) regulates caspase activation of gasdermin-D/E pores leading to secretion and extracellular activity of 3CL(pro).
Grin PM, Baid K, de Jesus HCR, Kozarac N, Bell PA, Jiang SZ, Kappelhoff R, Butler GS, Leborgne NGF, Pan C, Pablos I, Machado Y, Vederas JC, Kim H, Benarafa C, Banerjee A, Overall CM
Cell Rep. 2024 Dec 24;43(12):115080. doi: 10.1016/j.celrep.2024.115080. Epub 2024 Dec 12.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 218229 | pcDNA3.1-GSDMD_1-270 | Gasdermin1-270 is a pore-forming GSDMD formed by SARS-CoV-2 3CLpro cleavage. Transfection in HEK293 cells showed GSDMD 1-270 pores secrete 3CLpro, nucleocapsid, and in excess, kill cells by pyroptosis |
| 218230 | pcDNA3.1-GSDMD_1-275 | Gasdermin-D_1-275 is a pore-forming GSDMD formed by cleavage by host caspases. Transfection in HEK293 cells showed GSDMD 1-275 pores secrete 3CLpro, nucleocapsid; in excess, kills cells by pyroptosis |
| 218231 | pcDNA3.1-GSDMD_29-484 | Gasdermin-D_29-484 is a non-functional GSDMD formed by SARS-CoV-2 3CLpro cleavage in the N-terminal domain. Transfection in HEK293 cells showed no pore-forming activity or induction of pyroptosis |
| 218232 | pcDNA3.1-GSDMD_29-270 | Gasdermin-D_29-270 is a non-functional GSDMD formed by two SARS-CoV-2 3CLpro cleavages. Transfection in HEK293 cells showed no pore-forming activity or induction of pyroptosis |
| 224697 | pET21b(+)_SARS-CoV-2_3CLpro_Q306A-HIS | Bacterial Expression and Purification of SARS-CoV-2 3CLpro active protease with His-tag |
| 224698 | pET21b(+)_SARS-CoV-2_3CLpro_C145A-HIS | Bacterial Expression and Purification of SARS-CoV-2 3CLpro inactive protease with His-tag |
| 224701 | pcDNA3.1-GSDMD_H270A | Expression of mutated Gasdermin D_H270A (GSDMD_H270A) in transfected mammalian cell lines |
| 224702 | pcDNA3.1-GSDMD_Q29A | Expression of mutated Gasdermin D_Q29A (GSDMD_Q29A) in transfected mammalian cell lines |
| 224703 | pcDNA3.1-GSDMD_Q29A_H270A | Expression of mutant Gasdermin D_Q29A_H270A (GSDMD_Q29A_H270A) in transfected mammalian cell lines |
| 224704 | pcDNA3.1-GSDMD_D275A | Expression of mutated Gasdermin D_D275A (GSDMD_D275A) in transfected mammalian cell lines |
| 224705 | pcDNA3.1-GSDMD_H270A_D275A | Expression of mutated Gasdermin D_H270A_D275A (GSDMD_D275A) in transfected mammalian cell lines |