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Thomas Martin Lab: Martin lab plasmids

Unpublished

Plasmids from Article

ID Plasmid Purpose
244988CAPS1 HAExpression and purification of rat wild-type CAPS1
244989CAPS1 G476E HAExpression and purification of rat CAPS1 G476E mutant
244990CAPS1 Flag Apex2Proximity labeling
244991CAPS1 mKate2Distribution and localization of rat wild-type CAPS1 in cells
244992CAPS1 R944L Flag Apex2Bipolar rat CAPS1 R944L mutant used for proximity labeling studies
244993CAPS1 G476E Flag Apex2Proximity labeling studies for CAPS1 dysfunction and secretory trafficking
244994CAPS1 R944L mKate2Rat bipolar mutant CAPS1 R994L
244996EGFP Munc13-4Distribution and localization of human wild-type Munc13-4 in cells
244998emiRFP 670 shRNA Resistant Munc13-4Distribution and localization of human wild-type Munc13-4 in cells
244999mCherry shRNA Resistant Munc13-4Distribution and localization of human wild-type Munc13-4 in cells
245000ptwin strep HRV3C Flag Munc13-4 C2ABCalcium binding defective human Munc13-4 mutant
245001DsRed1 Munc13-4Distribution and localization of human wild-type Munc13-4 in cells
245005Munc13-1 Flag Apex2Proximity labeling studies for rat wild-type Munc13-1
245006Munc13-1 emiRFP 670Distributon and localization of rat wild-type Munc13-1
245007Munc13-1 mScarlet1 twin strepProtein purification and distribution and localization of rat wild-type Munc13-1 in cells
245008Flag Apex2 Munc13-4Proximity labeling
245009Munc13-1 GFPDistribution and localization and protein purification of rat wild-type Munc13-1 in cells
245010mApple Rab3aDistribution and localization of rat wild-typeRab3a
245011mApple Rab3bDistribution and localization of rat wild-type Rab3b
245012Rab3a Q81L BiosensorMeasuring GTPase activity of positive control
245013twin strep HRV3C Flag Rab3aProtein expression and purification of rat wild-type Rab3a
245014EGFP Rab3a T36NDistribution and localization of rat Rab3a T36N mutant
245015Rab3 T36N BiosensorMeasuring GTPase activity of negative control
245016EGFP Rab3a IDF/A3Effector binding mutant of rat Rab3a
245017Myc Rab3dDistribution and localization of rat wild-type Rab3d
245018emiRFP 670 Rab3a Q81LDistribution and localization of rat Rab3a Q81L mutant
245019Rab3a BiosensorMeasuring GTPase activity of Rab3
245020EGFP Rab3a F59SEffector binding rat Rab3a F59S mutant
245021mCherry Rab27aDistribution and localization of human wild-type Rab27a in cells
245022EGFP human Rab27a isoform-1 T23NDominant negative human Rab27a T23N mutant
245023mApple Rab27a iso-1 T23Nhuman Rab27a T23N mutant
245024EGFP Rab27a Q78Lhuman Rab27a Q78L mutant
245025mApple Rab27aDistribution and localization of human wild-type Rab27a in cells
245026Nterm Apex4 3xFlag Rab27aSplit Apex4 works with Nterm Apex human wild-type Rab27a
245029mCherry Rab2a Q41LConstitutively active human Rab2a Q41L mutant
245030mCherry VPS37bProtein localization of human wild-type VPS37b in cells
245031EGFP VPS39Protein localization of human wild-type VPS39 in cells
245032GFP VPS41Protein localization of human wild-type VPS41 in cells
245033mCherry VPS37cProtein localization of human wild-type VPS37c in cells
245034NPY EGFPSecretory granule marker for endocrine cells
245035NPY HaloSecretory granule marker for for endocrine cells
245036NPY td-tomatoSecretory granule marker for endocrine cells
245037NPY mTagBFP2Secretory granule marker for endocrine cells
245038mCgA EGFPSecretory granule marker of endocrine cells
245039 TRPML1-EGFPExpresses TRPML1 with C-terminus EGFP tag in mammalian cells
245040TRPML1 Y499A-EGFPExpresses TRPML1 mutant with C-terminus EGFP tag in mammalian cells
245041TRPML1 V432P mScarlet-I Expresses TRPML1 mutant with C-terminus mScarlet-I tag in mammalian cells
245042LAMP1 mScarlet twin strepLysosomal purification of lysosome marker LAMP1-mScarlet-twin-strep
245043LAMP1 3xFlag twin strepLysosomal purification of lysosome marker LAMP1-3xFlag-twin-strep
245045Phogrin mAppleSecretory granule marker using Phogrin mApple as marker
245046Phogrin EGFP twin strepSecretory granule purification using Phogrin EGFP-twin-strep marker
245047GFP Slyp4aRab effector
245048pEmerald RPH3A C3Rab effector
245049mCherry hSyntaxin11Distribution and localization of human wild-type syntaxin 11 in cells
245050GFP Rab3 GAP1Distribution and localization of human wild-type GAP1 in cells
245051mEmerald MADDDistribution and localization of human wild-type MADD in cells
245052BDNF mCherryMammalian secretory granule marker using BDNF mCherry marker
245053BDNF GFPMammalian secretory granule marker using BDNF GFP marker
245054ANF emiRFP 670 Mammalian secretory granule marker using ANF emiRFP marker
245055EGFP-HA PIKFYVE K1831EKinase-dead mutant
245056mApple TBC1D10BDistribution and localization of human TBC1D10B in cells
245057Myc tagged SNAP25b Sequence2Distribution and localization of synthetic wild-type SNAP25 in cells
245058twin-step HRV3C PLEKHM1Expression and purification of human wild-type PLEKHM1 in cells
245059mRuby2 RIMS2Distribution and localization of mouse wild-type RIMS2 in cells

Antibodies from Article