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Calarco Lab CRISPR Plasmids Available from Addgene

A CRISPR/Cas system designed and optimized for work in C. elegans .

The protocol for customizing the sgRNA expression plasmid can be located in the Materials and Methods section (online only) and Supplemental Material (online only) of the publication. For questions regarding the protocol please contact Dr. John Calarco: [email protected]

These plasmids are descirbed in:

Heritable genome editing in C. elegans via a CRISPR-Cas9 system. Friedland AE, Tzur YB, Esvelt KM, Colaiacovo MP, Church GM, Calarco JA. Nat. Methods. 2013 Jun. Nature Methods. PubMed PMID 23817069

Individual plasmids can be ordered via the links below:

ID Plasmid
46168 Peft-3::cas9-SV40_NLS::tbb-23'UTR: codon optimized Cas9_SV40 NLS with intron.
46169 PU6::unc-119_sgRNA: unc-119targeting sgRNA.
46170 PU6::klp-12_sgRNA: klp-12targeting sgRNA.

Please note: eft-3 has officially been changed to eef-1A.1 Please see the eef-1A.1 WormBase entryfor details.