Large-scale screening for novel low-affinity extracellular protein interactions.
Bushell KM, Sollner C, Schuster-Boeckler B, Bateman A, Wright GJ
Genome Res. 2008 Apr;18(4):622-30. Epub 2008 Feb 22.
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PubMed
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Article
AVEXIS is an avidity-based extracellular interaction screen to detect protein interactions, where the extracellular regions of receptor proteins are expressed as both monomeric baits and multimerised enzyme-tagged preys.
The Basic AVEXIS palsmid set is all that you will need to start producing proteins for AVEXIS screening. It includes negative control bait and preys (containing the Cd4 tag alone) and the CD200 and CD200 receptor bait and prey plasmids as a positive control. Please see the following reference for more information:
- A benchmarked protein microarray-based platform for the identification of novel low-affinity extracellular protein interactions Sun Y et al., Analytical Biochem 2012 May 1;424(1):45-53. PubMed 22342946
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 32402 | CD4d3+4-bio | |
| 32403 | CD4d3+4-blac | |
| 32404 | CD200Cd4d3+4-bio | A positive control receptor bait (rat CD4d3+4-bio) and prey (rat CD200) EXPRESs plasmid for The Basic AVEXIS kit |
| 32405 | CD200Cd4d3+4-blac | A positive control receptor bait (rat CD4d3+4-blac) and prey (rat CD200) EXPRESs plasmid for The Basic AVEXIS kit |
| 32406 | CD200RCd4d3+4-bio | A positive control receptor bait (rat CD4d3+4-bio) and prey (rat CD200 receptor) EXPRESs plasmid for The Basic AVEXIS kit |
| 32407 | CD200RCd4d3+4-blac | A positive control receptor bait (rat CD4d3+4-blac) and prey (rat CD200 receptor) EXPRESs plasmid for The Basic AVEXIS kit |
| 32408 | secretedBirA-8his | |
| 36152 | CD200CD4d3+4-His | A positive control receptor bait with His-tag (rat CD4d3+4-HIS) and prey (rat CD200) EXPRESs plasmid |
| 36153 | CD200RCD4d3+4-bio-linker-His | A positive control receptor bait with bio-linker-His (rat CD4d3+4-bio-linker-his) and prey (rat CD200 receptor) EXPRESs plasmid |
| 64395 | secreted BirA-Flag | Flag-tagged biotinylation enzyme for co-expression with bio-proteins |
| 71471 | CD200-Cd4d3+4-COMP-blac-3xFLAG-6his | A positive control receptor prey (rat CD200) EXPRESs plasmid for The Basic AVEXIS kit |