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Hsa21 Gene Expression Set
(Kit # 1000000099 )

Depositing Lab:   Roger Reeves

Trisomy for human chromosome 21 (Hsa21) results in Down syndrome (DS) which is among the most complex genetic perturbations compatible with survival past term. To advance understanding of DS, the Reeves lab has subcloned 163 individual cDNAs (representing 162 conserved genes from Hsa21) into the pCS2-DEST vector to create an expression-ready clone set.

Individual plasmids from this collection can be utilized for in vitro transcription from the SP6 promoter or transient expression from the CMV promoter in mammalian cells, and the entire clone set is compatible for mass cloning into other vectors.

This kit will be sent as bacterial glycerol stocks in 96-well plate format.

Original Publication

Citation pending

Description

Trisomy for human chromosome 21 (Hsa21) results in Down syndrome (DS) which is among the most complex genetic perturbations compatible with survival past term. While trisomy affects development of every tissue, reduced cognitive ability in DS is among the most limiting features and DS is one of the leading genetic causes of intellectual disability.

Because dosage imbalance in DS is present from conception onward, it is of interest to interrogate the effects of individual and combinatorial gene over-expression at early stages of development, which is difficult in mammalian models. The zebrafish, however, provides a tractable system for a large-scale screen of gene effects on early embryogenesis.

RNAs transcribed in vitro from this cDNA cloneset were injected into zebrafish embryos and individuals were screened for effects on early development (Edie SE et al, manuscript in revision). Several cDNAs consistently resulted in phenotypes accordant with down regulation of the shh pathway, and an additional clone repeatedly resulted in pericardial edema. The Reeves lab foresees the utility of this clone set for use in screens for other phenotypes in various cells and tissues.

How to Cite this Kit

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods of your future publications.

For your Materials and Methods section:

“Hsa21 Gene Expression Set was a gift from Roger Reeves (Addgene Kit #1000000099).”

For your Reference section:

Citation pending

Hsa21 Gene Expression Set - #1000000099

Resistance Color Key

Each circle corresponds to a specific antibiotic resistance in the kit plate map wells.

Inventory

Searchable and sortable table of all plasmids in kit. The Well column links to its kit plate map on the right. The Plasmid column links to a plasmid's individual web page.

Kit Plate Map

96-well plate map for plasmid layout. Hovering over a well reveals the plasmid name, while clicking on a well opens the plasmid page.

Resistance Color Key

Ampicillin

Inventory

Well Plasmid Resistance
Plate 1 / A / 1 TPTE_pCSdest
Ampicillin
Plate 1 / A / 2 CLIC6_pCSdest
Ampicillin
Plate 1 / A / 3 SIM2_pCSdest
Ampicillin
Plate 1 / A / 4 DYRK1A_pCSdest
Ampicillin
Plate 1 / A / 5 SAMSN1_pCSdest
Ampicillin
Plate 1 / A / 6 CHODL_pCSdest
Ampicillin
Plate 1 / A / 7 CYYR1_pCSdest
Ampicillin
Plate 1 / A / 8 CCT8_pCSdest
Ampicillin
Plate 1 / A / 9 HSF2BP_pCSdest
Ampicillin
Plate 1 / A / 10 SCAF4_pCSdest
Ampicillin
Plate 1 / A / 11 C21orf59_pCSdest
Ampicillin
Plate 1 / A / 12 IL10RB_pCSdest
Ampicillin
Plate 1 / B / 1 ITSN1_pCSdest
Ampicillin
Plate 1 / B / 2 BAGE_pCSdest
Ampicillin
Plate 1 / B / 3 Runx1_pCSdest
Ampicillin
Plate 1 / B / 4 HLCS_pCSdest
Ampicillin
Plate 1 / B / 5 KCNJ6_pCSdest
Ampicillin
Plate 1 / B / 6 NRIP1_pCSdest
Ampicillin
Plate 1 / B / 7 TMPRSS15_pCSdest
Ampicillin
Plate 1 / B / 8 ADAMTS1_pCSdest
Ampicillin
Plate 1 / B / 9 MAP3K7CL_pCSdest
Ampicillin
Plate 1 / B / 10 HUNK_pCSdest
Ampicillin
Plate 1 / B / 11 SYNJ1-145_pCSdest
Ampicillin
Plate 1 / B / 12 CLDN8_pCSdest
Ampicillin
Plate 1 / C / 1 ATP5O_pCSdest
Ampicillin
Plate 1 / C / 2 SETD4_pCSdest
Ampicillin
Plate 1 / C / 3 DSCR6_pCSdest
Ampicillin
Plate 1 / C / 4 DSCR4_pCSdest
Ampicillin
Plate 1 / C / 5 USP25_pCSdest
Ampicillin
Plate 1 / C / 6 NCAM2_pCSdest
Ampicillin
Plate 1 / C / 7 ADAMTS5_pCSdest
Ampicillin
Plate 1 / C / 8 BACH1_pCSdest
Ampicillin
Plate 1 / C / 9 MIS18A_pCSdest
Ampicillin
Plate 1 / C / 10 SYNJ1-170_pCSdest
Ampicillin
Plate 1 / C / 11 IFNGR2_pCSdest
Ampicillin
Plate 1 / C / 12 MRPS6_pCSdest
Ampicillin
Plate 1 / D / 1 BAGE4_pCSdest
Ampicillin
Plate 1 / D / 2 CBR1_pCSdest
Ampicillin
Plate 1 / D / 3 PIGP_pCSdest
Ampicillin
Plate 1 / D / 4 DSCR8_pCSdest
Ampicillin
Plate 1 / D / 5 LINC00478_pCSdest
Ampicillin
Plate 1 / D / 6 MRPL39_pCSdest
Ampicillin
Plate 1 / D / 7 LINC00314_pCSdest
Ampicillin
Plate 1 / D / 8 GRIK1_pCSdest
Ampicillin
Plate 1 / D / 9 MRAP_pCSdest
Ampicillin
Plate 1 / D / 10 PAXBP1_pCSdest
Ampicillin
Plate 1 / D / 11 TMEM50B_pCSdest
Ampicillin
Plate 1 / D / 12 SLC5A3_pCSdest
Ampicillin
Plate 1 / E / 1 ANKRD20A11P _pCSdest
Ampicillin
Plate 1 / E / 2 CBR3_pCSdest
Ampicillin
Plate 1 / E / 3 TMPRSS3_pCSdest
Ampicillin
Plate 1 / E / 4 KCNJ15_pCSdest
Ampicillin
Plate 1 / E / 5 C21orf37_pCSdest
Ampicillin
Plate 1 / E / 6 JAM2_pCSdest
Ampicillin
Plate 1 / E / 7 N6AMT1_pCSdest
Ampicillin
Plate 1 / E / 8 CLDN17_pCSdest
Ampicillin
Plate 1 / E / 9 C21orf119_pCSdest
Ampicillin
Plate 1 / E / 10 C21orf49_pCSdest
Ampicillin
Plate 1 / E / 11 KCNE2_pCSdest
Ampicillin
Plate 1 / E / 12 LIPI_pCSdest
Ampicillin
Plate 1 / F / 1 Dopey2_pCSdest
Ampicillin
Plate 1 / F / 2 TTC3_pCSdest
Ampicillin
Plate 1 / F / 3 DSCR10_pCSdest
Ampicillin
Plate 1 / F / 4 CXADR_pCSdest
Ampicillin
Plate 1 / F / 5 ATP5J_pCSdest
Ampicillin
Plate 1 / F / 6 LTN1_pCSdest
Ampicillin
Plate 1 / F / 7 WRB_pCSdest
Ampicillin
Plate 1 / F / 8 C21orf63_pCSdest
Ampicillin
Plate 1 / F / 9 OLIG2_pCSdest
Ampicillin
Plate 1 / F / 10 GART_pCSdest
Ampicillin
Plate 1 / F / 11 FAM165B_pCSdest
Ampicillin
Plate 1 / F / 12 RBM11_pCSdest
Ampicillin
Plate 1 / G / 1 CHAF1B_pCSdest
Ampicillin
Plate 1 / G / 2 DSCR9_pCSdest
Ampicillin
Plate 1 / G / 3 ERG_pCSdest
Ampicillin
Plate 1 / G / 4 BTG3_pCSdest
Ampicillin
Plate 1 / G / 5 RWDD2B_pCSdest
Ampicillin
Plate 1 / G / 6 TIAM1_pCSdest
Ampicillin
Plate 1 / G / 7 C21orf77_pCSdest
Ampicillin
Plate 1 / G / 8 OLIG1_pCSdest
Ampicillin
Plate 1 / G / 9 DONSON_pCSdest
Ampicillin
Plate 1 / G / 10 KCNE1_pCSdest
Ampicillin
Plate 1 / G / 11 HSPA13_pCSdest
Ampicillin
Plate 1 / G / 12 CLDN14_pCSdest
Ampicillin
Plate 1 / H / 1 DSCR3_pCSdest
Ampicillin
Plate 1 / H / 2 ETS2_pCSdest
Ampicillin
Plate 1 / H / 3 C21orf91_pCSdest
Ampicillin
Plate 1 / H / 4 APP_pCSdest
Ampicillin
Plate 1 / H / 5 USP16_pCSdest
Ampicillin
Plate 1 / H / 6 SOD1_pCSdest
Ampicillin
Plate 1 / H / 7 TCP10L_pCSdest
Ampicillin
Plate 1 / H / 8 IFNAR1_pCSdest
Ampicillin
Plate 1 / H / 9 CRYZL1_pCSdest
Ampicillin
Plate 1 / H / 10 RCAN1_pCSdest
Ampicillin
Plate 1 / H / 11 PSMG1_pCSdest
Ampicillin
Plate 1 / H / 12 DIP2A_pCSdest
Ampicillin
Plate 2 / A / 1 IGSF5_pCSdest
Ampicillin
Plate 2 / A / 2 TMPRSS2
Ampicillin
Plate 2 / A / 3 TFF3_pCSdest
Ampicillin
Plate 2 / A / 4 NDUFV3_pCSdest
Ampicillin
Plate 2 / A / 5 GABPA_pCSdest
Ampicillin
Plate 2 / A / 6 AIRE_pCSdest
Ampicillin
Plate 2 / A / 7 ITGB2_pCSdest
Ampicillin
Plate 2 / A / 8 COL6A1_pCSdest
Ampicillin
Plate 2 / A / 9 BRWD1_pCSdest
Ampicillin
Plate 2 / A / 10 S100B_pCSdest
Ampicillin
Plate 2 / A / 11 PCP4_pCSdest
Ampicillin
Plate 2 / A / 12 RIPK4_pCSdest
Ampicillin
Plate 2 / B / 1 TFF2_pCSdest
Ampicillin
Plate 2 / B / 2 PKNOX1_pCSdest
Ampicillin
Plate 2 / B / 3 PDXK_pCSdest
Ampicillin
Plate 2 / B / 4 PFKL_pCSdest
Ampicillin
Plate 2 / B / 5 COL6A2_pCSdest
Ampicillin
Plate 2 / B / 6 HMGN1_pCSdest
Ampicillin
Plate 2 / B / 7 PRMT2_pCSdest
Ampicillin
Plate 2 / B / 8 DSCAM_pCSdest
Ampicillin
Plate 2 / B / 9 PRDM15_pCSdest
Ampicillin
Plate 2 / B / 10 TFF1_pCSdest
Ampicillin
Plate 2 / B / 11 CBS_pCSdest
Ampicillin
Plate 2 / B / 12 CSTB_pCSdest
Ampicillin
Plate 2 / C / 1 C21orf2_pCSdest
Ampicillin
Plate 2 / C / 2 FAM207A _pCSdest
Ampicillin
Plate 2 / C / 3 FTCD_pCSdest
Ampicillin
Plate 2 / C / 4 IFNAR2_pCSdest
Ampicillin
Plate 2 / C / 5 BACE2_pCSdest
Ampicillin
Plate 2 / C / 6 C2CD2_pCSdest
Ampicillin
Plate 2 / C / 7 UBASH3A_pCSdest
Ampicillin
Plate 2 / C / 8 U2AF1_pCSdest
Ampicillin
Plate 2 / C / 9 RRP1_pCSdest
Ampicillin
Plate 2 / C / 10 TRPM2_pCSdest
Ampicillin
Plate 2 / C / 11 POFUT2_pCSdest
Ampicillin
Plate 2 / C / 12 C21orf56_pCSdest
Ampicillin
Plate 2 / D / 1 LCA5L_pCSdest
Ampicillin
Plate 2 / D / 2 PLAC4_pCSdest
Ampicillin
Plate 2 / D / 3 ZBTB21_pCSdest
Ampicillin
Plate 2 / D / 4 RSPH1_pCSdest
Ampicillin
Plate 2 / D / 5 CRYAA_pCSdest
Ampicillin
Plate 2 / D / 6 AGPAT3_pCSdest
Ampicillin
Plate 2 / D / 7 LRRC3_pCSdest
Ampicillin
Plate 2 / D / 8 Col18a1_pCSdest
Ampicillin
Plate 2 / D / 9 LSS_pCSdest
Ampicillin
Plate 2 / D / 10 SH3BGR_pCSdest
Ampicillin
Plate 2 / D / 11 FAM3B_pCSdest
Ampicillin
Plate 2 / D / 12 SLC37A1_pCSdest
Ampicillin
Plate 2 / E / 1 SIK1_pCSdest
Ampicillin
Plate 2 / E / 2 TRAPPC10_pCSdest
Ampicillin
Plate 2 / E / 3 UBE2G2_pCSdest
Ampicillin
Plate 2 / E / 4 ADARB1_pCSdest
Ampicillin
Plate 2 / E / 5 MCM3APlong_pCSdest
Ampicillin
Plate 2 / E / 6 B3GALT5_pCSdest
Ampicillin
Plate 2 / E / 7 MX2_pCSdest
Ampicillin
Plate 2 / E / 8 PDE9A_pCSdest
Ampicillin
Plate 2 / E / 9 PWP2_pCSdest
Ampicillin
Plate 2 / E / 10 SUMO3_pCSdest
Ampicillin
Plate 2 / E / 11 SLC19A1_pCSdest
Ampicillin
Plate 2 / E / 12 C21orf88_pCSdest
Ampicillin
Plate 2 / F / 1 MX1_pCSdest
Ampicillin
Plate 2 / F / 2 ABCG1_pCSdest
Ampicillin
Plate 2 / F / 3 WDR4_pCSdest
Ampicillin
Plate 2 / F / 4 LINC00313_pCSdest
Ampicillin
Plate 2 / F / 5 DNMT3L_pCSdest
Ampicillin
Plate 2 / F / 6 PTTG1IP_pCSdest
Ampicillin
Plate 2 / F / 7 PCBP3_pCSdest
Ampicillin
Plate 2 / F / 8 YBEY_pCSdest
Ampicillin
Data calculated @ 2018-04-24

Kit Plate Map - #1000000099

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