This website uses cookies to ensure you get the best experience. By continuing the use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

Plasmid kit icon

RAS Clone Collections- RAS and RAS Pathway Constructs
(Kit # 1000000070, 1000000089 )

Depositing Lab: Dominic Esposito

The Esposito Lab has provided 2 kits for both Ras wild type and mutant constructs.

Kit #1000000070

The RAS Pathway 2.0 Clone Collection is a set of vectors for use with the Gateway® Cloning Platform (Life Technologies) to permit construction of RAS pathway gene expression vectors for in vitro and in vivo research. While these clones can be used for standard Gateway cloning, their utility is greatly enhanced with the use of the FNLCR Combinatorial Cloning Platform (CCP) which allows simplified construction of vectors with different elements (see Wall VE et al, 2014 for more information).

Kit #1000000089

This collection contains 61 wild type and mutant RAS genes as Gateway entry clones. It is comprised of wild type HRAS, NRAS, KRAS4a and KRAS4b gene plasmids as well as different mutant versions of each gene.

These kits will be sent as bacterial glycerol stocks in a 96-well plate format.

Ordering

Item Catalog # Description Quantity Price (USD)
Kit 1000000070 RAS Pathway 2.0 Clone Collection- Wild type genes (360 constructs) 1 $1050 Add to Cart
Kit 1000000089 RAS Mutant Clone Collection- Wild type and mutant variations (61 constructs) 1 $375 Add to Cart

Description

Kit #1000000070

This collection contains 180 genes in the “RAS pathway version 2.0”, a crowdsourced pathway map of the RAS signaling pathway generated on the RASCentral website of the Frederick National Laboratory RAS Initiative. In each case, the primary splice variant was chosen to represent the most commonly found transcript by RNAseq data across all TCGA tumor samples. Many of these transcript variants are not available in cDNA or ORF libraries, or are available only in mutated forms. The sequences in this collection encode wild type proteins, and match the RefSeq data for the particular splice variants with the exception of known SNPs or cases in which the RefSeq data is not consistent with genome sequence data (see pathway map below).

Some uses of this system are:

  • Construction of protein expression constructs with various fusion tags
  • Generation of expression constructs with different promoters for in vivo expression
  • Production of clones with fluorescent tags for localization experiments
  • Generation of constructs for making mutant cell lines or transgenic animals
  • Production of vectors for shRNA or miRNA delivery

Kit #1000000089

This collection contains 61 wild type and mutant RAS genes as Gateway entry clones. It is comprised of wild type HRAS, NRAS, KRAS4a and KRAS4b gene plasmids as well as different mutant versions of each gene.


Schematic of the RAS pathway

Depositor Information

Kit #1000000070

Icon RAS Pathway Clone Collection 2.0 Overview (709.2 KB)

Icon Annotated Plasmid Maps (1.1 MB)

Gateway Recombinational Cloning

For more information on the Gateway system, please see:

DNA cloning using in vitro site-specific recombination. Hartley JL, Temple GF, Brasch MA. Genome Res.2000. Nov;10(11):1788-95. PubMed PMID 11076863

For more information on the FNLCR Combinatorial Cloning Platform (CCP):

Combinatorial assembly of clone libraries using site-specific recombination. Wall VE, Garvey LA, Mehalko JL, Procter LV, Esposito D. Methods Mol Biol. 2014;1116:193-208. doi: 10.1007/978-1-62703-764-8_14. PubMed PMID 24395366

How to Cite these Kits

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods of your future publications.

For your Materials and Methods section:

“The RAS Clone Collection was a gift from Dominic Esposito (Addgene kit # ).”

RAS Pathway 2.0 Clone Collection - #1000000070

Resistance Color Key

Each circle corresponds to a specific antibiotic resistance in the kit plate map wells.

Inventory

Searchable and sortable table of all plasmids in kit. The Well column links to its kit plate map on the right. The Plasmid column links to a plasmid's individual web page.

Kit Plate Map

96-well plate map for plasmid layout. Hovering over a well reveals the plasmid name, while clicking on a well opens the plasmid page.

Resistance Color Key

Spectinomycin
Kanamycin

Inventory

Well Plasmid Resistance
Plate 1 / A / 1 R777-E001 Hs.AKT1
Spectinomycin
Plate 1 / A / 2 R777-E002 Hs.AKT1-nostop
Spectinomycin
Plate 1 / A / 3 R777-E003 Hs.AKT2
Spectinomycin
Plate 1 / A / 4 R777-E004 Hs.AKT2-nostop
Spectinomycin
Plate 1 / A / 5 R777-E005 Hs.AKT3
Spectinomycin
Plate 1 / A / 6 R777-E006 Hs.AKT3-nostop
Spectinomycin
Plate 1 / A / 7 R777-E007 Hs.ALK
Spectinomycin
Plate 1 / A / 8 R777-E008 Hs.ALK-nostop
Spectinomycin
Plate 1 / A / 9 R777-E009 Hs.ARAF
Spectinomycin
Plate 1 / A / 10 R777-E010 Hs.ARAF-nostop
Spectinomycin
Plate 1 / A / 11 R777-E011 Hs.ARHGAP35
Spectinomycin
Plate 1 / A / 12 R777-E012 Hs.ARHGAP35-nostop
Spectinomycin
Plate 1 / B / 1 R777-E013 Hs.ARHGEF2
Spectinomycin
Plate 1 / B / 2 R777-E014 Hs.ARHGEF2-nostop
Spectinomycin
Plate 1 / B / 3 R777-E015 Hs.BRAF
Spectinomycin
Plate 1 / B / 4 R777-E016 Hs.BRAF-nostop
Spectinomycin
Plate 1 / B / 5 R777-E017 Hs.CCND1
Spectinomycin
Plate 1 / B / 6 R777-E018 Hs.CCND1-nostop
Spectinomycin
Plate 1 / B / 7 R777-E019 Hs.CCND2
Spectinomycin
Plate 1 / B / 8 R777-E020 Hs.CCND2-nostop
Spectinomycin
Plate 1 / B / 9 R777-E021 Hs.CCND3
Spectinomycin
Plate 1 / B / 10 R777-E022 Hs.CCND3-nostop
Spectinomycin
Plate 1 / B / 11 R777-E023 Hs.CDK4
Spectinomycin
Plate 1 / B / 12 R777-E024 Hs.CDK4-nostop
Spectinomycin
Plate 1 / C / 1 R777-E025 Hs.CDK6
Spectinomycin
Plate 1 / C / 2 R777-E026 Hs.CDK6-nostop
Spectinomycin
Plate 1 / C / 3 R777-E027 Hs.CNKSR1
Spectinomycin
Plate 1 / C / 4 R777-E028 Hs.CNKSR1-nostop
Spectinomycin
Plate 1 / C / 5 R777-E029 Hs.CNKSR2
Spectinomycin
Plate 1 / C / 6 R777-E030 Hs.CNKSR2-nostop
Spectinomycin
Plate 1 / C / 7 R777-E031 Hs.CYTH2
Spectinomycin
Plate 1 / C / 8 R777-E032 Hs.CYTH2-nostop
Spectinomycin
Plate 1 / C / 9 R777-E033 Hs.DUSP1
Spectinomycin
Plate 1 / C / 10 R777-E034 Hs.DUSP1-nostop
Spectinomycin
Plate 1 / C / 11 R777-E035 Hs.DUSP2
Spectinomycin
Plate 1 / C / 12 R777-E036 Hs.DUSP2-nostop
Spectinomycin
Plate 1 / D / 1 R777-E037 Hs.DUSP3
Spectinomycin
Plate 1 / D / 2 R777-E038 Hs.DUSP3-nostop
Spectinomycin
Plate 1 / D / 3 R777-E039 Hs.DUSP4
Spectinomycin
Plate 1 / D / 4 R777-E040 Hs.DUSP4-nostop
Spectinomycin
Plate 1 / D / 5 R777-E041 Hs.DUSP5
Spectinomycin
Plate 1 / D / 6 R777-E042 Hs.DUSP5-nostop
Spectinomycin
Plate 1 / D / 7 R777-E043 Hs.DUSP6
Spectinomycin
Plate 1 / D / 8 R777-E044 Hs.DUSP6-nostop
Spectinomycin
Plate 1 / D / 9 R777-E045 Hs.E2F1
Spectinomycin
Plate 1 / D / 10 R777-E046 Hs.E2F1-nostop
Spectinomycin
Plate 1 / D / 11 R777-E047 Hs.E2F2
Spectinomycin
Plate 1 / D / 12 R777-E048 Hs.E2F2-nostop
Spectinomycin
Plate 1 / E / 1 R777-E049 Hs.E2F3
Spectinomycin
Plate 1 / E / 2 R777-E050 Hs.E2F3-nostop
Spectinomycin
Plate 1 / E / 3 R777-E051 Hs.ECT2
Spectinomycin
Plate 1 / E / 4 R777-E052 Hs.ECT2-nostop
Spectinomycin
Plate 1 / E / 5 R777-E053 Hs.EGFR
Spectinomycin
Plate 1 / E / 6 R777-E054 Hs.EGFR-nostop
Spectinomycin
Plate 1 / E / 7 R777-E055 Hs.EIF4EBP1
Spectinomycin
Plate 1 / E / 8 R777-E056 Hs.EIF4EBP1-nostop
Spectinomycin
Plate 1 / E / 9 R777-E057 Hs.EIF4EBP2
Spectinomycin
Plate 1 / E / 10 R777-E058 Hs.EIF4EBP2-nostop
Spectinomycin
Plate 1 / E / 11 R777-E059 Hs.EIF4EBP3
Spectinomycin
Plate 1 / E / 12 R777-E060 Hs.EIF4EBP3-nostop
Spectinomycin
Plate 1 / F / 1 R777-E061 Hs.ERBB2
Spectinomycin
Plate 1 / F / 2 R777-E062 Hs.ERBB2-nostop
Spectinomycin
Plate 1 / F / 3 R777-E063 Hs.ETS1
Spectinomycin
Plate 1 / F / 4 R777-E064 Hs.ETS1-nostop
Spectinomycin
Plate 1 / F / 5 R777-E065 Hs.ETS2
Spectinomycin
Plate 1 / F / 6 R777-E066 Hs.ETS2-nostop
Spectinomycin
Plate 1 / F / 7 R777-E067 Hs.EXOC1
Spectinomycin
Plate 1 / F / 8 R777-E068 Hs.EXOC1-nostop
Spectinomycin
Plate 1 / F / 9 R777-E069 Hs.EXOC2
Spectinomycin
Plate 1 / F / 10 R777-E070 Hs.EXOC2-nostop
Spectinomycin
Plate 1 / F / 11 R777-E071 Hs.EXOC3
Spectinomycin
Plate 1 / F / 12 R777-E072 Hs.EXOC3-nostop
Spectinomycin
Plate 1 / G / 1 R777-E073 Hs.EXOC4
Spectinomycin
Plate 1 / G / 2 R777-E074 Hs.EXOC4-nostop
Spectinomycin
Plate 1 / G / 3 R777-E075 Hs.EXOC5
Spectinomycin
Plate 1 / G / 4 R777-E076 Hs.EXOC5-nostop
Spectinomycin
Plate 1 / G / 5 R777-E077 Hs.EXOC6
Spectinomycin
Plate 1 / G / 6 R777-E078 Hs.EXOC6-nostop
Spectinomycin
Plate 1 / G / 7 R777-E079 Hs.EXOC7
Spectinomycin
Plate 1 / G / 8 R777-E080 Hs.EXOC7-nostop
Spectinomycin
Plate 1 / G / 9 R777-E081 Hs.EXOC8
Spectinomycin
Plate 1 / G / 10 R777-E082 Hs.EXOC8-nostop
Spectinomycin
Plate 1 / G / 11 R777-E083 Hs.FGFR1
Spectinomycin
Plate 1 / G / 12 R777-E084 Hs.FGFR1-nostop
Spectinomycin
Plate 1 / H / 1 R777-E085 Hs.FGFR2
Spectinomycin
Plate 1 / H / 2 R777-E086 Hs.FGFR2-nostop
Spectinomycin
Plate 1 / H / 3 R777-E087 Hs.FGFR3
Spectinomycin
Plate 1 / H / 4 R777-E088 Hs.FGFR3-nostop
Spectinomycin
Plate 1 / H / 5 R777-E089 Hs.FGFR4
Spectinomycin
Plate 1 / H / 6 R777-E090 Hs.FGFR4-nostop
Spectinomycin
Plate 1 / H / 7 R777-E091 Hs.FLT3
Spectinomycin
Plate 1 / H / 8 R777-E092 Hs.FLT3-nostop
Spectinomycin
Plate 1 / H / 9 R777-E093 Hs.FNTA
Spectinomycin
Plate 1 / H / 10 R777-E094 Hs.FNTA-nostop
Spectinomycin
Plate 1 / H / 11 R777-E095 Hs.FNTB
Spectinomycin
Plate 1 / H / 12 R777-E096 Hs.FNTB-nostop
Spectinomycin
Plate 2 / A / 1 R777-E193 Hs.PRKAA2
Spectinomycin
Plate 2 / A / 2 R777-E194 Hs.PRKAA2-nostop
Spectinomycin
Plate 2 / A / 3 R777-E195 Hs.PRKAB1
Spectinomycin
Plate 2 / A / 4 R777-E196 Hs.PRKAB1-nostop
Spectinomycin
Plate 2 / A / 5 R777-E197 Hs.PRKAB2
Spectinomycin
Plate 2 / A / 6 R777-E198 Hs.PRKAB2-nostop
Spectinomycin
Plate 2 / A / 7 R777-E199 Hs.PRKAG1
Spectinomycin
Plate 2 / A / 8 R777-E200 Hs.PRKAG1-nostop
Spectinomycin
Plate 2 / A / 9 R777-E201 Hs.PRKAG2
Spectinomycin
Plate 2 / A / 10 R777-E202 Hs.PRKAG2-nostop
Spectinomycin
Plate 2 / A / 11 R777-E203 Hs.PRKAG3
Spectinomycin
Plate 2 / A / 12 R777-E204 Hs.PRKAG3-nostop
Spectinomycin
Plate 2 / B / 1 R777-E205 Hs.PTEN
Spectinomycin
Plate 2 / B / 2 R777-E206 Hs.PTEN-nostop
Spectinomycin
Plate 2 / B / 3 R777-E207 Hs.RAC1
Spectinomycin
Plate 2 / B / 4 R777-E208 Hs.RAC1-nostop
Spectinomycin
Plate 2 / B / 5 R777-E209 Hs.RAC2
Spectinomycin
Plate 2 / B / 6 R777-E210 Hs.RAC2-nostop
Spectinomycin
Plate 2 / B / 7 R777-E211 Hs.RAC3
Spectinomycin
Plate 2 / B / 8 R777-E212 Hs.RAC3-nostop
Spectinomycin
Plate 2 / B / 9 R777-E213 Hs.RAF1
Spectinomycin
Plate 2 / B / 10 R777-E214 Hs.RAF1-nostop
Spectinomycin
Plate 2 / B / 11 R777-E215 Hs.RALA
Spectinomycin
Plate 2 / B / 12 R777-E216 Hs.RALA-nostop
Spectinomycin
Plate 2 / C / 1 R777-E217 Hs.RALB
Spectinomycin
Plate 2 / C / 2 R777-E218 Hs.RALB-nostop
Spectinomycin
Plate 2 / C / 3 R777-E219 Hs.RALBP1
Spectinomycin
Plate 2 / C / 4 R777-E220 Hs.RALBP1-nostop
Spectinomycin
Plate 2 / C / 5 R777-E221 Hs.RALGDS
Spectinomycin
Plate 2 / C / 6 R777-E222 Hs.RALGDS-nostop
Spectinomycin
Plate 2 / C / 7 R777-E223 Hs.RAPGEF1
Spectinomycin
Plate 2 / C / 8 R777-E224 Hs.RAPGEF1-nostop
Spectinomycin
Plate 2 / C / 9 R777-E225 Hs.RAPGEF2
Spectinomycin
Plate 2 / C / 10 R777-E226 Hs.RAPGEF2-nostop
Spectinomycin
Plate 2 / C / 11 R777-E227 Hs.RASA1
Spectinomycin
Plate 2 / C / 12 R777-E228 Hs.RASA1-nostop
Spectinomycin
Plate 2 / D / 1 R777-E229 Hs.RASA2
Spectinomycin
Plate 2 / D / 2 R777-E230 Hs.RASA2-nostop
Spectinomycin
Plate 2 / D / 3 R777-E231 Hs.RASA3
Spectinomycin
Plate 2 / D / 4 R777-E232 Hs.RASA3-nostop
Spectinomycin
Plate 2 / D / 5 R777-E233 Hs.RASAL1
Spectinomycin
Plate 2 / D / 6 R777-E234 Hs.RASAL1-nostop
Spectinomycin
Plate 2 / D / 7 R777-E235 Hs.RASAL2
Spectinomycin
Plate 2 / D / 8 R777-E236 Hs.RASAL2-nostop
Spectinomycin
Plate 2 / D / 9 R777-E237 Hs.RASAL3
Spectinomycin
Plate 2 / D / 10 R777-E238 Hs.RASAL3-nostop
Spectinomycin
Plate 2 / D / 11 R777-E239 Hs.RASGRF1
Spectinomycin
Plate 2 / D / 12 R777-E240 Hs.RASGRF1-nostop
Spectinomycin
Plate 2 / E / 1 R777-E241 Hs.RASGRF2
Spectinomycin
Plate 2 / E / 2 R777-E242 Hs.RASGRF2-nostop
Spectinomycin
Plate 2 / E / 3 R777-E243 Hs.RASGRP1
Spectinomycin
Plate 2 / E / 4 R777-E244 Hs.RASGRP1-nostop
Spectinomycin
Plate 2 / E / 5 R777-E245 Hs.RASGRP2
Spectinomycin
Plate 2 / E / 6 R777-E246 Hs.RASGRP2-nostop
Spectinomycin
Plate 2 / E / 7 R777-E247 Hs.RASGRP3
Spectinomycin
Plate 2 / E / 8 R777-E248 Hs.RASGRP3-nostop
Spectinomycin
Plate 2 / E / 9 R777-E249 Hs.RASGRP4
Spectinomycin
Plate 2 / E / 10 R777-E250 Hs.RASGRP4-nostop
Spectinomycin
Plate 2 / E / 11 R777-E251 Hs.RASSF1
Spectinomycin
Plate 2 / E / 12 R777-E252 Hs.RASSF1-nostop
Spectinomycin
Plate 2 / F / 1 R777-E253 Hs.RASSF10
Spectinomycin
Plate 2 / F / 2 R777-E254 Hs.RASSF10-nostop
Spectinomycin
Plate 2 / F / 3 R777-E255 Hs.RASSF2
Spectinomycin
Plate 2 / F / 4 R777-E256 Hs.RASSF2-nostop
Spectinomycin
Plate 2 / F / 5 R777-E257 Hs.RASSF3
Spectinomycin
Plate 2 / F / 6 R777-E258 Hs.RASSF3-nostop
Spectinomycin
Plate 2 / F / 7 R777-E259 Hs.RASSF4
Spectinomycin
Plate 2 / F / 8 R777-E260 Hs.RASSF4-nostop
Spectinomycin
Plate 2 / F / 9 R777-E261 Hs.RASSF5
Spectinomycin
Plate 2 / F / 10 R777-E262 Hs.RASSF5-nostop
Spectinomycin
Plate 2 / F / 11 R777-E263 Hs.RASSF6
Spectinomycin
Plate 2 / F / 12 R777-E264 Hs.RASSF6-nostop
Spectinomycin
Plate 2 / G / 1 R777-E265 Hs.RASSF7
Spectinomycin
Plate 2 / G / 2 R777-E266 Hs.RASSF7-nostop
Spectinomycin
Plate 2 / G / 3 R777-E267 Hs.RASSF8
Spectinomycin
Plate 2 / G / 4 R777-E268 Hs.RASSF8-nostop
Spectinomycin
Plate 2 / G / 5 R777-E269 Hs.RASSF9
Spectinomycin
Plate 2 / G / 6 R777-E270 Hs.RASSF9-nostop
Spectinomycin
Plate 2 / G / 7 R777-E271 Hs.RB1
Spectinomycin
Plate 2 / G / 8 R777-E272 Hs.RB1-nostop
Spectinomycin
Plate 2 / G / 9 R777-E273 Hs.RCE1
Spectinomycin
Plate 2 / G / 10 R777-E274 Hs.RCE1-nostop
Spectinomycin
Plate 2 / G / 11 R777-E275 Hs.RHEB
Spectinomycin
Plate 2 / G / 12 R777-E276 Hs.RHEB-nostop
Spectinomycin
Plate 2 / H / 1 R777-E277 Hs.RHOA
Spectinomycin
Plate 2 / H / 2 R777-E278 Hs.RHOA-nostop
Spectinomycin
Plate 2 / H / 3 R777-E279 Hs.RHOB
Spectinomycin
Plate 2 / H / 4 R777-E280 Hs.RHOB-nostop
Spectinomycin
Plate 2 / H / 5 R777-E281 Hs.RHOC
Spectinomycin
Plate 2 / H / 6 R777-E282 Hs.RHOC-nostop
Spectinomycin
Plate 2 / H / 7 R777-E283 Hs.ROCK1
Spectinomycin
Plate 2 / H / 8 R777-E284 Hs.ROCK1-nostop
Spectinomycin
Plate 2 / H / 9 R777-E285 Hs.ROCK2
Spectinomycin
Plate 2 / H / 10 R777-E286 Hs.ROCK2-nostop
Spectinomycin
Plate 2 / H / 11 R777-E287 Hs.ROS1
Spectinomycin
Plate 2 / H / 12 R777-E288 Hs.ROS1-nostop
Spectinomycin
Plate 3 / A / 1 R777-E097 Hs.FOS
Spectinomycin
Plate 3 / A / 2 R777-E098 Hs.FOS-nostop
Spectinomycin
Plate 3 / A / 3 R777-E099 Hs.GRB2
Spectinomycin
Plate 3 / A / 4 R777-E100 Hs.GRB2-nostop
Spectinomycin
Plate 3 / A / 5 R777-E101 Hs.HRAS
Spectinomycin
Plate 3 / A / 6 R777-E102 Hs.HRAS-nostop
Spectinomycin
Plate 3 / A / 7 R777-E103 Hs.ICMT
Spectinomycin
Plate 3 / A / 8 R777-E104 Hs.ICMT-nostop
Spectinomycin
Plate 3 / A / 9 R777-E105 Hs.INSR
Spectinomycin
Plate 3 / A / 10 R777-E106 Hs.INSR-nostop
Spectinomycin
Plate 3 / A / 11 R777-E107 Hs.INSRR
Spectinomycin
Plate 3 / A / 12 R777-E108 Hs.INSRR-nostop
Spectinomycin
Plate 3 / B / 1 R777-E109 Hs.IRS1
Spectinomycin
Plate 3 / B / 2 R777-E110 Hs.IRS1-nostop
Spectinomycin
Plate 3 / B / 3 R777-E111 Hs.IRS2
Spectinomycin
Plate 3 / B / 4 R777-E112 Hs.IRS2-nostop
Spectinomycin
Plate 3 / B / 5 R777-E113 Hs.JUN
Spectinomycin
Plate 3 / B / 6 R777-E114 Hs.JUN-nostop
Spectinomycin
Plate 3 / B / 7 R777-E115 Hs.KRAS
Spectinomycin
Plate 3 / B / 8 R777-E116 Hs.KRAS-nostop
Spectinomycin
Plate 3 / B / 9 R777-E117 Hs.KSR1
Spectinomycin
Plate 3 / B / 10 R777-E118 Hs.KSR1-nostop
Spectinomycin
Plate 3 / B / 11 R777-E119 Hs.KSR2
Spectinomycin
Plate 3 / B / 12 R777-E120 Hs.KSR2-nostop
Spectinomycin
Plate 3 / C / 1 R777-E121 Hs.MAP2K1
Spectinomycin
Plate 3 / C / 2 R777-E122 Hs.MAP2K1-nostop
Spectinomycin
Plate 3 / C / 3 R777-E123 Hs.MAP2K2
Spectinomycin
Plate 3 / C / 4 R777-E124 Hs.MAP2K2-nostop
Spectinomycin
Plate 3 / C / 5 R777-E125 Hs.MAPK1
Spectinomycin
Plate 3 / C / 6 R777-E126 Hs.MAPK1-nostop
Spectinomycin
Plate 3 / C / 7 R777-E127 Hs.MAPK3
Spectinomycin
Plate 3 / C / 8 R777-E128 Hs.MAPK3-nostop
Spectinomycin
Plate 3 / C / 9 R777-E129 Hs.MDM2
Spectinomycin
Plate 3 / C / 10 R777-E130 Hs.MDM2-nostop
Spectinomycin
Plate 3 / C / 11 R777-E131 Hs.MET
Spectinomycin
Plate 3 / C / 12 R777-E132 Hs.MET-nostop
Spectinomycin
Plate 3 / D / 1 R777-E133 Hs.MLST8
Spectinomycin
Plate 3 / D / 2 R777-E134 Hs.MLST8-nostop
Spectinomycin
Plate 3 / D / 3 R777-E135 Hs.MRAS
Spectinomycin
Plate 3 / D / 4 R777-E136 Hs.MRAS-nostop
Spectinomycin
Plate 3 / D / 5 R777-E137 Hs.MTOR
Spectinomycin
Plate 3 / D / 6 R777-E138 Hs.MTOR-nostop
Spectinomycin
Plate 3 / D / 7 R777-E139 Hs.NF1
Spectinomycin
Plate 3 / D / 8 R777-E140 Hs.NF1-nostop
Spectinomycin
Plate 3 / D / 9 R777-E141 Hs.NFE2L2
Spectinomycin
Plate 3 / D / 10 R777-E142 Hs.NFE2L2-nostop
Spectinomycin
Plate 3 / D / 11 R777-E143 Hs.NFKB1
Spectinomycin
Plate 3 / D / 12 R777-E144 Hs.NFKB1-nostop
Spectinomycin
Plate 3 / E / 1 R777-E145 Hs.NRAS
Spectinomycin
Plate 3 / E / 2 R777-E146 Hs.NRAS-nostop
Spectinomycin
Plate 3 / E / 3 R777-E147 Hs.PAK1
Spectinomycin
Plate 3 / E / 4 R777-E148 Hs.PAK1-nostop
Spectinomycin
Plate 3 / E / 5 R777-E149 Hs.PAK2
Spectinomycin
Plate 3 / E / 6 R777-E150 Hs.PAK2-nostop
Spectinomycin
Plate 3 / E / 7 R777-E151 Hs.PAK3
Kanamycin
Plate 3 / E / 8 R777-E152 Hs.PAK3-nostop
Spectinomycin
Plate 3 / E / 9 R777-E153 Hs.PAK4
Spectinomycin
Plate 3 / E / 10 R777-E154 Hs.PAK4-nostop
Spectinomycin
Plate 3 / E / 11 R777-E155 Hs.PDGFRA
Spectinomycin
Plate 3 / E / 12 R777-E156 Hs.PDGFRA-nostop
Spectinomycin
Plate 3 / F / 1 R777-E157 Hs.PDGFRB
Spectinomycin
Plate 3 / F / 2 R777-E158 Hs.PDGFRB-nostop
Spectinomycin
Plate 3 / F / 3 R777-E159 Hs.PDPK1
Spectinomycin
Plate 3 / F / 4 R777-E160 Hs.PDPK1-nostop
Spectinomycin
Plate 3 / F / 5 R777-E161 Hs.PEBP1
Spectinomycin
Plate 3 / F / 6 R777-E162 Hs.PEBP1-nostop
Spectinomycin
Plate 3 / F / 7 R777-E163 Hs.PIK3CA
Spectinomycin
Plate 3 / F / 8 R777-E164 Hs.PIK3CA-nostop
Spectinomycin
Plate 3 / F / 9 R777-E165 Hs.PIK3CB
Spectinomycin
Plate 3 / F / 10 R777-E166 Hs.PIK3CB-nostop
Spectinomycin
Plate 3 / F / 11 R777-E167 Hs.PIK3CD
Spectinomycin
Plate 3 / F / 12 R777-E168 Hs.PIK3CD-nostop
Spectinomycin
Plate 3 / G / 1 R777-E169 Hs.PIK3CG
Spectinomycin
Plate 3 / G / 2 R777-E170 Hs.PIK3CG-nostop
Spectinomycin
Plate 3 / G / 3 R777-E171 Hs.PIK3R1
Spectinomycin
Plate 3 / G / 4 R777-E172 Hs.PIK3R1-nostop
Spectinomycin
Plate 3 / G / 5 R777-E173 Hs.PIK3R2
Spectinomycin
Plate 3 / G / 6 R777-E174 Hs.PIK3R2-nostop
Spectinomycin
Plate 3 / G / 7 R777-E175 Hs.PIK3R3
Spectinomycin
Plate 3 / G / 8 R777-E176 Hs.PIK3R3-nostop
Spectinomycin
Plate 3 / G / 9 R777-E177 Hs.PIK3R4
Spectinomycin
Plate 3 / G / 10 R777-E178 Hs.PIK3R4-nostop
Spectinomycin
Plate 3 / G / 11 R777-E179 Hs.PIK3R5
Spectinomycin
Plate 3 / G / 12 R777-E180 Hs.PIK3R5-nostop
Spectinomycin
Plate 3 / H / 1 R777-E181 Hs.PIK3R6
Spectinomycin
Plate 3 / H / 2 R777-E182 Hs.PIK3R6-nostop
Spectinomycin
Plate 3 / H / 3 R777-E183 Hs.PIN1
Spectinomycin
Plate 3 / H / 4 R777-E184 Hs.PIN1-nostop
Spectinomycin
Plate 3 / H / 5 R777-E185 Hs.PLXNB1
Spectinomycin
Plate 3 / H / 6 R777-E186 Hs.PLXNB1-nostop
Spectinomycin
Plate 3 / H / 7 R777-E187 Hs.PPP1CA
Spectinomycin
Plate 3 / H / 8 R777-E188 Hs.PPP1CA-nostop
Spectinomycin
Plate 3 / H / 9 R777-E189 Hs.PREX2
Spectinomycin
Plate 3 / H / 10 R777-E190 Hs.PREX2-nostop
Spectinomycin
Plate 3 / H / 11 R777-E191 Hs.PRKAA1
Spectinomycin
Plate 3 / H / 12 R777-E192 Hs.PRKAA1-nostop
Spectinomycin
Plate 4 / A / 1 R777-E289 Hs.RPS6KA1
Spectinomycin
Plate 4 / A / 2 R777-E290 Hs.RPS6KA1-nostop
Spectinomycin
Plate 4 / A / 3 R777-E291 Hs.RPS6KA2
Spectinomycin
Plate 4 / A / 4 R777-E292 Hs.RPS6KA2-nostop
Spectinomycin
Plate 4 / A / 5 R777-E293 Hs.RPS6KA3
Spectinomycin
Plate 4 / A / 6 R777-E294 Hs.RPS6KA3-nostop
Spectinomycin
Plate 4 / A / 7 R777-E295 Hs.RPS6KA6
Spectinomycin
Plate 4 / A / 8 R777-E296 Hs.RPS6KA6-nostop
Spectinomycin
Plate 4 / A / 9 R777-E297 Hs.RPS6KB1
Spectinomycin
Plate 4 / A / 10 R777-E298 Hs.RPS6KB1-nostop
Spectinomycin
Plate 4 / A / 11 R777-E299 Hs.RPS6KB2
Spectinomycin
Plate 4 / A / 12 R777-E300 Hs.RPS6KB2-nostop
Spectinomycin
Plate 4 / B / 1 R777-E301 Hs.RPTOR
Spectinomycin
Plate 4 / B / 2 R777-E302 Hs.RPTOR-nostop
Spectinomycin
Plate 4 / B / 3 R777-E303 Hs.SAV1
Spectinomycin
Plate 4 / B / 4 R777-E304 Hs.SAV1-nostop
Spectinomycin
Plate 4 / B / 5 R777-E305 Hs.SCRIB
Spectinomycin
Plate 4 / B / 6 R777-E306 Hs.SCRIB-nostop
Spectinomycin
Plate 4 / B / 7 R777-E307 Hs.SHC1
Spectinomycin
Plate 4 / B / 8 R777-E308 Hs.SHC1-nostop
Spectinomycin
Plate 4 / B / 9 R777-E309 Hs.SHC2
Spectinomycin
Plate 4 / B / 10 R777-E310 Hs.SHC2-nostop
Spectinomycin
Plate 4 / B / 11 R777-E311 Hs.SHC3
Spectinomycin
Plate 4 / B / 12 R777-E312 Hs.SHC3-nostop
Spectinomycin
Plate 4 / C / 1 R777-E313 Hs.SHC4
Spectinomycin
Plate 4 / C / 2 R777-E314 Hs.SHC4-nostop
Spectinomycin
Plate 4 / C / 3 R777-E315 Hs.SHOC2
Spectinomycin
Plate 4 / C / 4 R777-E316 Hs.SHOC2-nostop
Spectinomycin
Plate 4 / C / 5 R777-E317 Hs.SOS1
Spectinomycin
Plate 4 / C / 6 R777-E318 Hs.SOS1-nostop
Spectinomycin
Plate 4 / C / 7 R777-E319 Hs.SOS2
Spectinomycin
Plate 4 / C / 8 R777-E320 Hs.SOS2-nostop
Spectinomycin
Plate 4 / C / 9 R777-E321 Hs.SPRED1
Spectinomycin
Plate 4 / C / 10 R777-E322 Hs.SPRED1-nostop
Spectinomycin
Plate 4 / C / 11 R777-E323 Hs.SPRED2
Spectinomycin
Plate 4 / C / 12 R777-E324 Hs.SPRED2-nostop
Spectinomycin
Plate 4 / D / 1 R777-E325 Hs.SPRED3
Spectinomycin
Plate 4 / D / 2 R777-E326 Hs.SPRED3-nostop
Spectinomycin
Plate 4 / D / 3 R777-E327 Hs.SPRY1
Spectinomycin
Plate 4 / D / 4 R777-E328 Hs.SPRY1-nostop
Spectinomycin
Plate 4 / D / 5 R777-E329 Hs.SPRY2
Spectinomycin
Plate 4 / D / 6 R777-E330 Hs.SPRY2-nostop
Spectinomycin
Plate 4 / D / 7 R777-E331 Hs.SPRY3
Spectinomycin
Plate 4 / D / 8 R777-E332 Hs.SPRY3-nostop
Spectinomycin
Plate 4 / D / 9 R777-E334 Hs.SPRY4-nostop
Spectinomycin
Plate 4 / D / 10 R777-E333 Hs.SPRY4
Spectinomycin
Plate 4 / D / 11 R777-E335 Hs.STK11
Spectinomycin
Plate 4 / D / 12 R777-E336 Hs.STK11-nostop
Spectinomycin
Plate 4 / E / 1 R777-E337 Hs.STK3
Spectinomycin
Plate 4 / E / 2 R777-E338 Hs.STK3-nostop
Spectinomycin
Plate 4 / E / 3 R777-E339 Hs.STK4
Spectinomycin
Plate 4 / E / 4 R777-E340 Hs.STK4-nostop
Spectinomycin
Plate 4 / E / 5 R777-E341 Hs.TBK1
Spectinomycin
Plate 4 / E / 6 R777-E342 Hs.TBK1-nostop
Spectinomycin
Plate 4 / E / 7 R777-E343 Hs.TFDP1
Spectinomycin
Plate 4 / E / 8 R777-E344 Hs.TFDP1-nostop
Spectinomycin
Plate 4 / E / 9 R777-E345 Hs.TFDP2
Spectinomycin
Plate 4 / E / 10 R777-E346 Hs.TFDP2-nostop
Spectinomycin
Plate 4 / E / 11 R777-E347 Hs.TIAM1
Spectinomycin
Plate 4 / E / 12 R777-E348 Hs.TIAM1-nostop
Spectinomycin
Plate 4 / F / 1 R777-E349 Hs.TIAM2
Spectinomycin
Plate 4 / F / 2 R777-E350 Hs.TIAM2-nostop
Spectinomycin
Plate 4 / F / 3 R777-E351 Hs.TP53
Spectinomycin
Plate 4 / F / 4 R777-E352 Hs.TP53-nostop
Spectinomycin
Plate 4 / F / 5 R777-E353 Hs.TSC1
Spectinomycin
Plate 4 / F / 6 R777-E354 Hs.TSC1-nostop
Spectinomycin
Plate 4 / F / 7 R777-E355 Hs.TSC2
Spectinomycin
Plate 4 / F / 8 R777-E356 Hs.TSC2-nostop
Spectinomycin
Plate 4 / F / 9 R777-E357 Hs.VAV1
Spectinomycin
Plate 4 / F / 10 R777-E358 Hs.VAV1-nostop
Spectinomycin
Plate 4 / F / 11 R777-E359 Hs.YAP1
Spectinomycin
Plate 4 / F / 12 R777-E360 Hs.YAP1-nostop
Spectinomycin
Data cached @ 2017-03-25

Kit Plate Map - #1000000070

RAS mutant clone collection - #1000000089

Resistance Color Key

Each circle corresponds to a specific antibiotic resistance in the kit plate map wells.

Inventory

Searchable and sortable table of all plasmids in kit. The Well column links to its kit plate map on the right. The Plasmid column links to a plasmid's individual web page.

Kit Plate Map

96-well plate map for plasmid layout. Hovering over a well reveals the plasmid name, while clicking on a well opens the plasmid page.

Resistance Color Key

Spectinomycin

Inventory

Well Plasmid Resistance
A / 1 Hs.KRAS4b
Spectinomycin
A / 2 Hs.KRAS4b G12C
Spectinomycin
A / 3 Hs.KRAS4b G12D
Spectinomycin
A / 4 Hs.KRAS4b G12V
Spectinomycin
A / 5 Hs.KRAS4b G13D
Spectinomycin
A / 6 Hs.KRAS4b Q61L
Spectinomycin
A / 7 Hs.KRAS4b Q61R
Spectinomycin
A / 8 Hs.KRAS4b S181A
Spectinomycin
A / 9 Hs.KRAS4b S181D
Spectinomycin
A / 10 Hs.KRAS4b S181E
Spectinomycin
A / 11 Hs.KRAS4b C185S
Spectinomycin
A / 12 Hs.KRAS4b Q61H
Spectinomycin
B / 1 Hs.KRAS4b M188L
Spectinomycin
B / 2 Hs.KRAS4b G12A
Spectinomycin
B / 3 Hs.KRAS4b G12R
Spectinomycin
B / 4 Hs.KRAS4b G12S
Spectinomycin
B / 5 Hs.KRAS4b G13C
Spectinomycin
B / 6 Hs.KRAS4b K117N
Spectinomycin
B / 7 Hs.KRAS4b A146V
Spectinomycin
B / 8 Hs.KRAS4b R68S
Spectinomycin
B / 9 Hs.KRAS4b G12F
Spectinomycin
B / 10 Hs.KRAS4b A146T
Spectinomycin
B / 11 Hs.KRAS4b Y64A
Spectinomycin
B / 12 Hs.KRAS4b K104A
Spectinomycin
C / 1 Hs.KRAS4b K104Q
Spectinomycin
C / 2 Hs.KRAS4b K147L
Spectinomycin
C / 3 Hs.KRAS4b K147Q
Spectinomycin
C / 4 Hs.KRAS4b S17N
Spectinomycin
C / 5 Hs.KRAS4b T35S
Spectinomycin
C / 6 Hs.KRAS4b E37G
Spectinomycin
C / 7 Hs.KRAS4b Y40C
Spectinomycin
C / 8 Hs.KRAS4b M72C
Spectinomycin
C / 9 Hs.KRAS4b Y40F
Spectinomycin
C / 10 Hs.KRAS4b R73E
Spectinomycin
C / 11 Hs.KRAS4b R73K
Spectinomycin
C / 12 Hs.KRAS4b Q61K
Spectinomycin
D / 1 Hs.KRAS4b G12D/C185S
Spectinomycin
D / 2 Hs.KRAS4a
Spectinomycin
D / 3 Hs.KRAS4a G12C
Spectinomycin
D / 4 Hs.KRAS4a G12D
Spectinomycin
D / 5 Hs.KRAS4a G12V
Spectinomycin
D / 6 Hs.KRAS4a G13D
Spectinomycin
D / 7 Hs.KRAS4a Q61L
Spectinomycin
D / 8 Hs.KRAS4a Q61R
Spectinomycin
D / 9 Hs.NRAS
Spectinomycin
D / 10 Hs.NRAS G12V
Spectinomycin
D / 11 Hs.NRAS G12C
Spectinomycin
D / 12 Hs.NRAS G12D
Spectinomycin
E / 1 Hs.NRAS G13D
Spectinomycin
E / 2 Hs.NRAS Q61L
Spectinomycin
E / 3 Hs.NRAS Q61R
Spectinomycin
E / 4 Hs.NRAS Q61K
Spectinomycin
E / 5 Hs.HRAS
Spectinomycin
E / 6 Hs.HRAS G12C
Spectinomycin
E / 7 Hs.HRAS G12D
Spectinomycin
E / 8 Hs.HRAS G12V
Spectinomycin
E / 9 Hs.HRAS G13D
Spectinomycin
E / 10 Hs.HRAS Q61L
Spectinomycin
E / 11 Hs.HRAS Q61K
Spectinomycin
E / 12 Hs.HRAS Q61R
Spectinomycin
F / 1 Hs.HRAS C186S
Spectinomycin
Data cached @ 2017-03-25

Kit Plate Map - #1000000089

Content blocked, you may need to disable your ad-blocker.