Skip to main content
Addgene
Showing: 1 - 20 of 4182 results

  1. pAR17_StrepI_hFASh_H8_pET22b

    Plasmid
    #122846
    Purpose
    Expresses human type I fatty acid synthase (FASN) in Escherichia coli. N-terminal Twin-Strep tag; C-terminal H8-tag; in pET22b vector.
    Depositor
    Martin Grininger
    Article
    Rittner et al Protein Sci. 2019 Feb;28(2):414-428. doi
    Insert
    human FASN/ human fatty acid synthase (FASN Human)
    Use
    Tags
    His8 tag and Twin-Strep tag
    Expression
    Bacterial
    Mutation
    wildtype
    Promoter
    T7 promoter
    Available since
    March 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    122846-plasmid-map-sequence-id-238539

    Enlarge

  2. pET22b_PstS_1

    Plasmid
    #78198
    Purpose
    Expression of single-cysteine variant of mature phosphate binding protein, PstS, as scaffold for phosphate biosensor
    Depositor
    Martin Webb
    Article
    Solscheid et al Biochemistry. 2015 Aug 18;54(32):5054-62
    Insert
    pstS (A197C)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    A197C (please see depositors comment below)
    Promoter
    T7
    Available since
    July 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    78198-plasmid-map-sequence-id-146048

    Enlarge

  3. pET22b_PstS_3

    Plasmid
    #78200
    Purpose
    Expression of double-cysteine variant of mature phosphate binding protein, PstS, which also has a mutation to weaken phosphate binding, as scaffold for phosphate biosensor
    Depositor
    Martin Webb
    Article
    Solscheid et al Biochemistry. 2015 Aug 18;54(32):5054-62
    Insert
    pstS (A17C,I76G,A197C)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    A17C,I76G,A197C (please see depositor comment bel…
    Promoter
    T7
    Available since
    July 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    78200-plasmid-map-sequence-id-146050

    Enlarge

  4. pET22b_PstS_2

    Plasmid
    #78199
    Purpose
    Expression of double-cysteine variant of mature phosphate binding protein, PstS, as scaffold for phosphate biosensor
    Depositor
    Martin Webb
    Article
    Solscheid et al Biochemistry. 2015 Aug 18;54(32):5054-62
    Insert
    pstS (A17C,A197C)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    A17C; A197C (please see depositor comment below)
    Promoter
    T7
    Available since
    July 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    78199-plasmid-map-sequence-id-146049

    Enlarge

  6. Pet28a_Human_H3.1

    Plasmid
    #42631
    Depositor
    Joe Landry
    Insert
    Human H3.1 (H3C1 Human)
    Use
    Tags
    Untagged
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 25, 2013
    Availability
    Industry, Academic Institutions, and Nonprofits
    42631-plasmid-map-sequence-id-57746

    Enlarge

  8. pET28b-XI

    Plasmid
    #157988
    Purpose
    Expresses XI in E.coli cell
    Depositor
    Byung Il Lee
    Insert
    xylose isomerase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    157988-plasmid-map-sequence-id-309115

    Enlarge

  12. pET22B_SA_PcrA

    Plasmid
    #102999
    Purpose
    The plasmid is used to express Staphylococcus aureus PcrA, a DNA helicase, with N-terminal His-tag and Gly-Gly linker
    Depositor
    Martin Webb
    Insert
    SA_PcrA
    Use
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    PcrA 199-2423
    Promoter
    T7
    Available since
    May 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    102999-plasmid-map-sequence-id-199646

    Enlarge

  20. Pet28a_Human_H4

    Plasmid
    #42633
    Depositor
    Joe Landry
    Insert
    Human H4 (H4C1 Human)
    Use
    Tags
    Untagged
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 25, 2013
    Availability
    Industry, Academic Institutions, and Nonprofits
    42633-plasmid-map-sequence-id-57748

    Enlarge

Showing: 1 - 20 of 4182 results