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pCMV-GCaMP5G

Plasmid

#31788

Purpose

a single-wavelength GCaMP3-based calcium indicator with improved response. Please also see the GCaMP6s/m/f indicators.

Depositor

Douglas Kim, Loren Looger, GENIE Project

Article

Akerboom et al J Neurosci. 2012 Oct 3;32(40):13819-40.

Insert

GCaMP5G

Tags

6xHis, T7 epitope, and Xpress tag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 22, 2011

Availability

Academic Institutions and Nonprofits only

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pCAGGS-GCaMP2

Plasmid

#18926

Depositor

Karel Svoboda

Article

Mao et al PLoS ONE. 2008 . 3(3):e1796.

Insert

GCaMP2

Tags

His

Expression

Mammalian

Available Since

Aug. 13, 2008

Availability

Academic Institutions and Nonprofits only
CMV::ratSyGCaMP2

Plasmid

#26124

Depositor

Leon Lagnado

Article

Dreosti et al Nat Methods. 2009 Dec . 6(12):883-9.

Insert

Synaptophysin GCaMP2 (Syp Rat)

Expression

Mammalian

Available Since

Nov. 11, 2010

Availability

Academic Institutions and Nonprofits only

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nGCaMP2-NpuDnaEn

Plasmid

#34850

Depositor

Kevin Truong

Article

Wong et al Cell Calcium. 2011 Nov 29.

Insert

nGCaMP2-NpuDnaEn

Expression

Bacterial and Mammalian

Promoter

CMV, T7 lac

Available Since

March 27, 2012

Availability

Academic Institutions and Nonprofits only
NpuDnaEc-cGCaMP2

Plasmid

#34851

Depositor

Kevin Truong

Article

Wong et al Cell Calcium. 2011 Nov 29.

Insert

NpuDnaEc-cGCaMP2

Expression

Bacterial and Mammalian

Promoter

CMV, T7 lac

Available Since

June 21, 2012

Availability

Academic Institutions and Nonprofits only
CMV-GCaMP2

Plasmid

#18927

Depositor

Karel Svoboda

Article

Mao et al PLoS ONE. 2008 . 3(3):e1796.

Insert

GCaMP2

Tags

His

Expression

Mammalian

Available Since

Aug. 13, 2008

Availability

Academic Institutions and Nonprofits only
pSADdeltaG-GCaMP3

Plasmid

#32644

Depositor

Edward Callaway

Article

Osakada et al Neuron. 2011 Aug 25;71(4):617-31.

Insert

GCaMP3

Use

Rabies virus

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 18, 2011

Availability

Academic Institutions and Nonprofits only

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pGP-CMV-GCaMP6f

Plasmid

#40755

Purpose

Mammalian expression of ultrasensitive protein calcium sensor

Depositor

Douglas Kim, GENIE Project

Article

Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

Insert

GCaMP6f

Tags

6xHis, T7 epitope, and Xpress tag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 29, 2012

Availability

Academic Institutions and Nonprofits only

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pGP-CMV-GCaMP6s

Plasmid

#40753

Purpose

Mammalian expression of ultrasensitive protein calcium sensor

Depositor

Douglas Kim, GENIE Project

Article

Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

Insert

GCaMP6s

Tags

6xHis, T7 epitope, and Xpress tag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pGP-CMV-GCaMP6m

Plasmid

#40754

Purpose

Mammalian expression of ultrasensitive protein calcium sensor

Depositor

Douglas Kim, GENIE Project

Article

Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

Insert

GCaMP6m

Tags

6xHis, T7 epitope, and Xpress tag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 29, 2012

Availability

Academic Institutions and Nonprofits only

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I-SceI pB 5UAS::ZfSyGCaMP2

Plasmid

#26125

Depositor

Leon Lagnado

Article

Dreosti et al Nat Methods. 2009 Dec . 6(12):883-9.

Insert

Synaptophysin GCaMP2 (sypb Zebrafish)

Use

Zebrafish expression

Tags

GCaMP2

Available Since

Oct. 18, 2010

Availability

Academic Institutions and Nonprofits only

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pN1 Lck-GCaMP5G

Plasmid

#34924

Purpose

Expresses a fusion between the Lck domain and cytosolic GCaMP5G. This construct targets GCaMP5G to the plasma membrane

Depositor

Baljit Khakh

Article

Akerboom et al J Neurosci. 2012 Oct 3;32(40):13819-40.

Insert

Lck-GCaMP5G

Expression

Mammalian

Mutation

Plasmid contains N-terminal 26 amino acid membran…

Promoter

CMV

Available Since

Feb. 21, 2012

Availability

Academic Institutions and Nonprofits only

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pN1-Lck-GCaMP3

Plasmid

#26974

Depositor

Baljit Khakh

Article

Shigetomi et al Neuron Glia Biol. 2010 Dec 16:1-9. [Epub

Insert

Lck-GCaMP3

Tags

His and Xpress

Expression

Mammalian

Mutation

Plasmid contains N-terminal 26 amino acid membran…

Available Since

Jan. 27, 2011

Availability

Academic Institutions and Nonprofits only

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pN1-Lck-GCaMP2

Plasmid

#24794

Depositor

Baljit Khakh

Article

Shigetomi et al Nat Neurosci. 2010 Jun;13(6):759-66.

Insert

Lck-GCaMP2

Tags

His and Xpress

Expression

Mammalian

Mutation

Plasmid contains N-terminal 26 amino acid membran…

Available Since

June 14, 2010

Availability

Academic Institutions and Nonprofits only

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pCAGGS-PSD95-GCaMP2

Plasmid

#18931

Depositor

Karel Svoboda

Article

Mao et al PLoS ONE. 2008 . 3(3):e1796.

Insert

PSD95-GCaMP2 (Dlg4 Rat)

Tags

His

Expression

Mammalian

Available Since

Sept. 3, 2008

Availability

Academic Institutions and Nonprofits only
pCAGGS-GCaMP2-actin

Plasmid

#18928

Depositor

Karel Svoboda

Article

Mao et al PLoS ONE. 2008 . 3(3):e1796.

Insert

GCaMP2-Actin (ACTB Chicken)

Tags

His

Expression

Mammalian

Available Since

Sept. 3, 2008

Availability

Academic Institutions and Nonprofits only
pSADdeltaG-GCaMP3-DsRedX

Plasmid

#32645

Depositor

Edward Callaway

Article

Osakada et al Neuron. 2011 Aug 25;71(4):617-31.

Inserts

GCaMP3
DsRedX

Use

Rabies virus

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 26, 2011

Availability

Academic Institutions and Nonprofits only
pCAGGS-hCD4-GCaMP2

Plasmid

#18932

Depositor

Karel Svoboda

Article

Mao et al PLoS ONE. 2008 . 3(3):e1796.

Insert

hCD4-GCaMP2 (CD4 Human)

Tags

His

Expression

Mammalian

Available Since

Sept. 3, 2008

Availability

Academic Institutions and Nonprofits only
Cx36-GCaMP

Plasmid

#123604

Purpose

Mouse Connexin 36-GCaMP3 fusion for measuring gap junction localized calcium signaling - expresses in mammalian cells

Depositor

John O'Brien

Article

Moore et al eNeuro. 2020 Mar 11. pii: ENEURO.0445-19

Insert

Connexin 36-GCaMP3 fusion (Gjd2 Mouse)

Tags

GCaMP3

Expression

Mammalian

Promoter

CMV

Available Since

March 31, 2020

Availability

Academic Institutions and Nonprofits only

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pENN.AAV.CamKII.GCaMP6f.WPRE.SV40

Plasmid

#100834

Purpose

AAV expression of ultrasensitive protein calcium sensor from CamKII promoter

Depositor

James M. Wilson

Has Service

AAV1, AAV5, and AAV9

Insert

GCaMP6f

Use

AAV

Expression

Mammalian

Mutation

GCaMP3-T302L R303P A317E D380Y T381R S383T R392G

Promoter

CamKII

Available Since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

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