Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 279 results for: nhei

Clear keywords
Showing: 1 - 20 of 279 results

  9. pLenti_mpx_AsCas12a(dual-gRNA)_h7SK(PacI-ClaI)_U6(I-SceI-NheI)_PGK-puro

    Plasmid
    #189635
    Purpose
    Lentiviral expression of double dual-AsCas12a gRNAs for generating combinatorial AsCas12a 3Cs libraries
    Depositor
    Manuel Kaulich
    Article
    Cetin et al Sci Rep. 2023 May 6;13(1):7405. doi: 10.
    Insert
    h7SK arrayed Cas12a gRNA cassette, hU6 arrayed Cas12a gRNA cassette, PGK puro cassette
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    Nov. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    189635-plasmid-map-sequence-id-414905

    Enlarge

  10. pRS424 Prp8 WT TRP1

    Plasmid
    #234120
    Purpose
    The N-terminus of Prp8 was restored thereby destroying the NheI site and correcting the ORF
    Depositor
    Aaron Hoskins
    Insert
    Prp8
    Expression
    Yeast
    Available Since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    234120-plasmid-map-sequence-id-464810

    Enlarge

  11. MZB117

    Plasmid
    #228481
    Purpose
    "Empty" C-terminal HRV-3C cleavable GST tag vector for rapid cloning/DNA Assembly via NheI restriction enzyme site (Bacterial Expression)
    Depositor
    Richard Baker
    Type
    Empty backbone
    Tags
    GST (glutathione S-transferase)
    Expression
    Bacterial
    Promoter
    T7 Promoter
    Available Since
    Jan. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    228481-plasmid-map-sequence-id-454022

    Enlarge

  12. pcDNA3.1_hSMAD4

    Plasmid
    #212710
    Purpose
    The ORF of human SMAD4, variant 1 cDNA amplified from the RT human liver RNA was cloned at the NheI/HindIII sites of the pcDNA3.1(+), the mammalian expression vector.
    Depositor
    Catharine Ross, Reza Zolfaghari
    Insert
    Homo sapiens SMAD4 (SMAD4 Human)
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    212710-plasmid-map-sequence-id-448252

    Enlarge

  13. pAAV-CBh-mKate2-IRES-MCS (WT-WT)

    Plasmid
    #105921
    Purpose
    AAV-mediated gene expression from CBh promoter. Contains canonical left and right AAV2 ITRs
    Depositor
    Marcella Patrick
    Has Service
    AAV2
    Inserts
    mKate2
    IRES-Multiple cloning site
    Use
    AAV
    Mutation
    codon optimized
    Promoter
    CBh and same CBh promoter as mKate2
    Available Since
    Feb. 15, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    105921-plasmid-map-sequence-id-443051

    Enlarge

  14. pAAV-CBh-mKate2-IRES-MCS (C-gap-WT)

    Plasmid
    #105922
    Purpose
    AAV-mediated gene expression from CBh promoter. Contains left AAV2 ITR with an 11bp deletion in C region and canonical right AAV2 ITR
    Depositor
    Marcella Patrick
    Inserts
    mKate2
    IRES-Multiple cloning site
    Use
    AAV
    Mutation
    codon optimized
    Promoter
    CBh and same CBh promoter as mKate2
    Available Since
    Feb. 15, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    105922-plasmid-map-sequence-id-200599

    Enlarge

  15. pcDNA3.1_hCAR.Variant3

    Plasmid
    #209133
    Purpose
    The ORF of human constitutive androstane receptor (CAR), variant 3 cDNA amplified from the RT human liver RNA was cloned at the NheI/HindIII sites of the pcDNA3.1(+), the mammalian expression vector.
    Depositor
    Catharine Ross, Reza Zolfaghari
    Insert
    constitutive androstane receptor (CAR), Variant 3 (NR1I3 Human)
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    209133-plasmid-map-sequence-id-422366

    Enlarge

  17. rFIXX-bp chain A (IRESneo)

    Plasmid
    #82450
    Purpose
    Chain A of the habu snake venom FIX/FX binding protein with a C-terminal polyhistidine-tag. Cloned into pIRESneo3 (Ampicillin) via 5' NheI and 3' BamHI; IRES vector conferring neomycin resistance.
    Depositor
    Robert Peters
    Insert
    rFIXX-bp chain A
    Tags
    Polyhistidine
    Expression
    Bacterial and Mammalian
    Promoter
    T7 RNA polymerase promoter
    Available Since
    Nov. 8, 2016
    Availability
    Academic Institutions and Nonprofits only
    82450-plasmid-map-sequence-id-156201

    Enlarge

  18. rFIXX-bp chain B (IRESpuro)

    Plasmid
    #82451
    Purpose
    Chain B of the habu snake venom FIX/FX binding protein with C-terminal biotin-tag. Cloned into pIRESneo3 (Ampicillin) via 5' NheI and 3' BamHI; IRES vector conferring puromycin resistance.
    Depositor
    Robert Peters
    Insert
    rFIXX-bp chain B
    Tags
    Biotin
    Expression
    Bacterial and Mammalian
    Promoter
    T7 RNA polymerase promoter
    Available Since
    Nov. 8, 2016
    Availability
    Academic Institutions and Nonprofits only
    82451-plasmid-map-sequence-id-156993

    Enlarge

Showing: 1 - 20 of 279 results