We narrowed to 279 results for: YPet
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Plasmid#118718PurposeThe donor (YPet(short)) only control for the no force control of the F40-based human desmoplakin II tension sensor provides the donor only lifetime used in lifetime measurements to determine FRET.DepositorInserthuman Desmoplakin II (1-1353)-[YPet(short)-F40-mCherry(Y72L)] (DSP Human)
UseRetroviralTagsYPet(short)-F40-mCherry(Y72L)ExpressionMammalianMutationtruncation after aa1353; Y72L mutation in mCherry…PromoterCMVAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
Desmoplakin II donor only control (FL-based tension sensor)
Plasmid#118723PurposeThe donor only (YPet(short)) control for the FL-based human desmoplakin II tension sensor provides the donor only lifetime used in fluorescence lifetime measurements to determine FRET.DepositorInserthuman Desmoplakin II-[YPet(short)-FL-mCherry(Y72L)] (internal-1353) (DSP Human)
UseRetroviralTagsYPet(short)-FL-mCherry(Y72L)ExpressionMammalianMutationinserted FL-based tension sensor module after aa1…PromoterCMVAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
PL-452 N-CyPet
Plasmid#19171DepositorInsertCyPet
UseCre/LoxAvailable sinceOct. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
PL-452 C-CyPet
Plasmid#19176DepositorInsertCyPet
UseCre/LoxAvailable sinceOct. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
pEB1-mYPet
Plasmid#103988PurposePlasmid encoding mYPetDepositorInsertmYPet
UseLow copyExpressionBacterialMutationMutations relative to wild-type GFP (F46L, I47L, …PromoterproCAvailable sinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJL1-CyPet
Plasmid#102636PurposeIn vitro expression of CyPet from the T7 promoterDepositorInsertCyPet
ExpressionBacterialPromoterT7Available sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
Desmoplakin II donor only control (FL-based no force control)
Plasmid#118722PurposeThe donor (YPet(short)) only control for the no force control of the FL-based human desmoplakin II tension sensor provides the donor only lifetime used in lifetime measurements to determine FRET.DepositorInserthuman Desmoplakin II (1-1353)-[YPet(short)-FL-mCherry(Y72L)] (DSP Human)
UseRetroviralTagsYPet(short)-FL-mCherry(Y72L)ExpressionMammalianMutationtruncation after aa1353; Y72L mutation in mCherry…PromoterCMVAvailable sinceDec. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
Desmoplakin II acceptor only control (F40-based tension sensor)
Plasmid#118719PurposeThe acceptor (mCherry) only control for the F40-based human desmoplakin II tension sensor can be co-expressed with the donor (YPet(short)) only control to determine intermolecular FRET.DepositorInserthuman Desmoplakin II-[YPet(short)(Y67G)-F40-mCherry] (internal-1353) (DSP Human)
UseRetroviralTagsYPet(short)(Y67G)-F40-mCherryExpressionMammalianMutationinserted F40-based tension sensor module after aa…PromoterCMVAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-ymYPET-URA3
Plasmid#168056PurposeReplaces Addgene plasmid 118460. Yeast tagging vector to create a gene fusion with yeast optimized mYPET with CaUra3 selection; Contains the linker region at the 5’ end of the FPDepositorInsertymYPET
TagsymYPETExpressionYeastAvailable sinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDRF1-GW ymYPET-T2A-mTurquoise2
Plasmid#118444PurposeProduces equimolar expression of ymYPET and mTq2DepositorInsertymYPET-T2A-mTurquoise2
ExpressionYeastMutationYPET A206K, F208S, E232L, N235DAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
GEF-H1 FLARE191.pa
Plasmid#134931PurposeGEF-H1 biosensor that has GEF activity.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGEF-H1(1-191)-yPet-4X linker-Cerulean3-GEF-H1(192-end)
TagsNone.ExpressionBacterial and MammalianPromoterCMV and T7Available sinceDec. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-IL-2 signal_CyPet
Plasmid#128029PurposeMammalian expression vector with N terminal IL-2 signal peptide and CyPetDepositorTypeEmpty backboneTagsCyPet and IL-2 signal peptideExpressionMammalianAvailable sinceAug. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
CYPet-Actin-3
Plasmid#55459PurposeLocalization: Actin, Excitation: 433, Emission: 475DepositorAvailable sinceOct. 2, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
CyPet-A206K-PRL2
Plasmid#149685PurposeFor the FRET-based binding assay between human CNNM3 CBS domain and PRL2.DepositorInsertCyPet-A206K-PRL2
Tags8 histidine tagExpressionBacterialMutationCyPet A206KPromoterT7Available sinceJune 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
CyPet-FKBP-E2
Plasmid#175244PurposeBacterial expression and purification, E2 enzyme (UBC9) for SUMOylation, can be recruited to FRB with rapamycinDepositorAvailable sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
CYPet-H2B-6
Plasmid#55461PurposeLocalization: Nucleus/Histones, Excitation: 433, Emission: 475DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertH2B (H2BC11 Human)
TagsCYPetExpressionMammalianMutationD26G and V119I in H2BPromoterCMVAvailable sinceOct. 2, 2014AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pcDNA3-C-terminal CyPet
Plasmid#128026PurposeMammalian expression vector with C terminal CyPet tagDepositorTypeEmpty backboneTagsCyPetExpressionMammalianAvailable sinceAug. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
EKAREN5-gl
Plasmid#225958PurposeThe YPet and mTurquoise sequence cassettes in EKAREN5(Addgene 167821) were replaced with a synonymous codon variant of YPet and mTurquoise-gl and subcloned into pCSII lentiviral backbone.DepositorInsertEKAREN5-gl
UseLentiviralTagsnls localization motifExpressionMammalianPromoterEF-1-alpha promoterAvailable sinceJan. 22, 2025AvailabilityAcademic Institutions and Nonprofits onlyCited by -
C122-E16: CyPet
Plasmid#162896PurposeStandard Gateway entry clone encoding CyPet fluorescent proteinDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCyPet fluorescent protein
UseSynthetic BiologyAvailable sinceApril 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
CYPet-CytERM-N-17
Plasmid#55460PurposeLocalization: Endoplasmic Reticulum, Excitation: 433, Emission: 475DepositorInsertCytERM
TagsCYPetExpressionMammalianPromoterCMVAvailable sinceOct. 2, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits