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Plasmid#124130PurposeBacterial expression plasmid for PURE system componentsDepositorInsertRelease factor 2 (prfB Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pQE60-RRF-His
Plasmid#124132PurposeBacterial expression plasmid for PURE system componentsDepositorInsertRibosome recycling factor (frr Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
mNeon-Vamp2
Plasmid#186619PurposeEncodes Vamp 2 fused to mNeonGreenDepositorInsertVesicle Associated Membrane Protein 2 (VAMP2 Human)
UseTagsmNeonGreenExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pVP141
Plasmid#222079PurposeSingle component CRISPR-mediated base-editor for Agrobacterium genetic engineering visually marked with sfGFP for guide insertion.DepositorInsertdCas9-PmCDA1, sfGFP and sacB
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceJan. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-mNeon-TurboID
Plasmid#186610PurposeThird generation lentiviral vector expressing mNeonGreen fused to V5-TurboIDDepositorInsertmNeon-V5-TurboID
UseLentiviralTagsmNeonGreenExpressionMammalianMutationFor reference see Branon et al., 2018PromoterCMVAvailable sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
Bongwoori-Pos6
Plasmid#101655PurposeBongwoori with the CC1-Pos6 linker length and composition.(* super ecliptic pHluorin A227D)DepositorInsertBongwoori-Pos6
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
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Rtn2b-mCherry
Plasmid#186600PurposeEncodes human full-length Reticulon 2 isoform B fused to mCherryDepositorInsertHuman Reticulon 2 isoform B (RTN2 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
Rtn2b-mNeon
Plasmid#186598PurposeEncodes human full-length Reticulon 2 isoform B fused to mNeonGreenDepositorInsertHuman Reticulon 2 isoform B (RTN2 Human)
UseTagsmNeonGreenExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
6xO/S luc
Plasmid#69445Purposedetection/assessment of OCT4 and SOX2 transcription factor activityDepositorInsertminimal fgf4 promoter and 6XOS tandem repeat of composite OCT4/Sox2 binding site from FGF4 enhancer
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceMay 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUC_sgRNA
Plasmid#68710PurposeContains the sgRNA backbone sequence (tracrRNA, 82 bp) and is used as DNA template to amplify a specific sgRNA using a forward primer with the protospacer sequence for gene targeting.DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMOTag-M9-4H
Plasmid#106379PurposeCan be used as a PCR template to amplify the M9-HA point mutant of glmS fragment for gene specific tagging that only allows tagging but not regulation. Acts as a control for glmS based gene regulationDepositorInsertGlmS with M9 mutation
UseTagsExpressionMutationPromoterAvailable sinceMarch 2, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
β2AR-PPI-CALPon1.0 (TEVp)
Plasmid#213407PurposePeroxidase (APEX) reporter for beta 2-adrenergic receptor (β2AR) and β-arrestin 2 interaction (component 2)DepositorInsertHA-arrestin-TEVp
UseLentiviralTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQE30-His-PheRS
Plasmid#124116PurposeBacterial expression plasmid for PURE system componentsDepositorUseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceJune 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET21a-GlyRS-His
Plasmid#124110PurposeBacterial expression plasmid for PURE system componentsDepositorUseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVP143
Plasmid#222080PurposeSingle component CRISPR-mediated base-editor for Agrobacterium genetic engineering visually marked with sfGFP for guide insertion and AeBlue for plasmid eviction.DepositorInsertdCas9-PmCDA1, sfGFP, sacB and AeBlue
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceJan. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVP144
Plasmid#222081PurposeSingle component CRISPR-mediated base-editor for Agrobacterium genetic engineering visually marked with sfGFP for guide insertion and eforRED for plasmid eviction.DepositorInsertdCas9-PmCDA1, sfGFP, sacB and eforRED
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceJan. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
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pPD005 pcDNA Golden Gate
Plasmid#138749PurposeVector used to express most COMET components, modified from pcDNA3.1/HygroR(+) for BsaI/BpiI Golden Gate compatibility and to lack HygroR.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
PP_072_pAAV_hSyn_DIO-LR-Voltron2-P2A-LR-CheRiff-HA
Plasmid#230988PurposeCo-expressing membrane-localized Voltron2 and membrane-localized CheRiff.DepositorInsertCre-dependent, membrane-localized optopatch for neuronal dendrites
UseAAVTagsExpressionMammalianMutationPromoterhSynAvailable sinceJune 5, 2025AvailabilityAcademic Institutions and Nonprofits only