Skip to main content
Addgene
Showing: 181 - 200 of 1383 results

  1. pAAV-4mtD3cpv

    Plasmid
    #181874
    Purpose
    Expresses the FRET-based mitochondrial calcium indicator 4mtD3cpv under control of the CaMKIIa promoter
    Depositor
    Hilmar Bading
    Article
    Hagenston et al J Biol Chem. 2021 Dec 20:101508. doi: 10
    Insert
    4mtD3cpv
    Use
    AAV and Adenoviral
    Tags
    Expression
    Mammalian
    Mutation
    the 4mtD3cpv CDS differs from the recorded GeneBa…
    Promoter
    CaMKIIa
    Available since
    March 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    181874-plasmid-map-sequence-id-355965

    Enlarge

  2. pAAV-shNCLX-2

    Plasmid
    #181871
    Purpose
    Expresses NCLX-targeted shRNA under control of the U6 promoter and mCherry under control of the CaMKIIa promoter
    Depositor
    Hilmar Bading
    Article
    Hagenston et al J Biol Chem. 2021 Dec 20:101508. doi: 10
    Insert
    solute carrier 8 family member B1 (Slc8b1 Mouse)
    Use
    AAV, Adenoviral, and Mouse Targeting
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    March 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    181871-plasmid-map-sequence-id-355961

    Enlarge

  3. pAAV-shNCLX-1

    Plasmid
    #181870
    Purpose
    Expresses NCLX-targeted shRNA under control of the U6 promoter and mCherry under control of the CaMKIIa promoter
    Depositor
    Hilmar Bading
    Article
    Hagenston et al J Biol Chem. 2021 Dec 20:101508. doi: 10
    Insert
    solute carrier 8 family member B1 (Slc8b1 Mouse)
    Use
    AAV, Adenoviral, and Mouse Targeting
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    March 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    181870-plasmid-map-sequence-id-355957

    Enlarge

  4. pAAV-EGFP.T2A.NCLX

    Plasmid
    #181873
    Purpose
    Expresses EGFP and murine NCLX (separated by a T2A cleavage site) under control of the synthetic CAG promoter
    Depositor
    Hilmar Bading
    Article
    Hagenston et al J Biol Chem. 2021 Dec 20:101508. doi: 10
    Inserts
    solute carrier family 8 member B1 (Slc8a1 Mouse)
    EGFP
    Use
    AAV and Adenoviral
    Tags
    HA and myc
    Expression
    Mammalian
    Mutation
    Promoter
    synthetic hybrid CAG promoter and synthetic hybri…
    Available since
    March 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    181873-plasmid-map-sequence-id-355952

    Enlarge

  5. pTV001

    Plasmid
    #130265
    Purpose
    EatA expression plasmid containing 8His encoding region in permissive site of eatA gene
    Depositor
    James Fleckenstein
    Article
    Sheikh et al Infect Immun. 2021 Nov 22:IAI0057221. do
    Insert
    eatA::His8
    Use
    Tags
    polyhistidine (internal)
    Expression
    Bacterial
    Mutation
    8His polyhistidine encoding region and XhoI site …
    Promoter
    araBAD
    Available since
    Nov. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    130265-plasmid-map-sequence-id-257613

    Enlarge

  6. pAAV-EGFP.T2A.NCLX.3'UTR

    Plasmid
    #181872
    Purpose
    Expresses EGFP and murine NCLX (separated by a T2A cleavage site) under control of the synthetic CAG promoter (includes a large portion of the Slc8b1 3'UTR)
    Depositor
    Hilmar Bading
    Article
    Hagenston et al J Biol Chem. 2021 Dec 20:101508. doi: 10
    Inserts
    solute carrier family 8 member B1 (Slc8b1 Mouse)
    EGFP
    3'UTR of solute carrier family 8 member B1
    Use
    AAV and Adenoviral
    Tags
    HA, T2A, and myc
    Expression
    Mammalian
    Mutation
    includes a large portion of the Slc8b1 3'UTR…
    Promoter
    synthetic hybrid CAG promoter
    Available since
    March 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    181872-plasmid-map-sequence-id-356018

    Enlarge

  7. pTW5126

    Plasmid
    #129732
    Purpose
    polyhistidine replacement of EatA passenger DUF corresponding to amino acids E541 -Q617 of the native EatA protein
    Depositor
    James Fleckenstein
    Article
    Sheikh et al Infect Immun. 2021 Nov 22:IAI0057221. do
    Insert
    eatA-DUF::His8
    Use
    Tags
    9His tag
    Expression
    Bacterial
    Mutation
    replaces the domain of unknown function correspon…
    Promoter
    araBAD
    Available since
    Nov. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    129732-plasmid-map-sequence-id-257612

    Enlarge

  8. pFloxed DHFR-TS*

    Plasmid
    #100605
    Purpose
    pUC19 with loxP-pDHFR-TS:DHFR-TS*(Pyr-R)-Stop-loxP-DHFR-TS 3' UTR
    Depositor
    David Sibley
    Insert
    Floxed TgDHFR-TS*
    Use
    Toxoplasma expression
    Tags
    Expression
    Mutation
    Pyrimethamine resistance
    Promoter
    Available since
    Sept. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    100605-plasmid-map-sequence-id-192861

    Enlarge

  9. pUPRT::Floxed DHFR-TS*

    Plasmid
    #100606
    Purpose
    TgUPRT disruption by knock in with Floxed DHFR-TS*(Pyr-R) cassette with UPRT homology flanks.
    Depositor
    David Sibley
    Insert
    TgUPRT::Floxed TgDHFR-TS*
    Use
    Toxoplasma expression
    Tags
    Expression
    Mutation
    Pyrimethamine resistance
    Promoter
    pTgDHFR-TS
    Available since
    Sept. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    100606-plasmid-map-sequence-id-192862

    Enlarge

  10. C39m

    Plasmid
    #120972
    Purpose
    MoClo golden gate assembly CD part for ccdB (DNA gyrase toxin, modified synonymously to remove BsaI cut site; ccdA/C79m is antitoxin). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    ccdB toxin
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI site removed
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120972-plasmid-map-sequence-id-236024

    Enlarge

  11. MIC2

    Plasmid
    #50050
    Purpose
    expresses toxoplasma gondii MIC2 extracellular domain in mammalian cells
    Depositor
    Timothy Springer
    Insert
    MIC2
    Use
    Tags
    HIS, SBP, and TEV
    Expression
    Mammalian
    Mutation
    S158A, N357S, N463S, N470D
    Promoter
    CMV
    Available since
    April 14, 2014
    Availability
    Academic Institutions and Nonprofits only

  12. m2ap

    Plasmid
    #50051
    Purpose
    expresses toxoplasma gondii MIC2 associated protein (m2ap) in mammalian cells
    Depositor
    Timothy Springer
    Insert
    M2AP
    Use
    Tags
    HIS
    Expression
    Mammalian
    Mutation
    starts at aa47; N127R, S158A, S177N, N357S, N463S…
    Promoter
    CMV
    Available since
    March 14, 2014
    Availability
    Academic Institutions and Nonprofits only

  13. Ubi:ccdb + RUBY

    Plasmid
    #233306
    Purpose
    GATEWAY compatible vector with soybean Ubiquitin (GmUbi) promoter driving in frame epitope fusion of ccdb selection toxin and RUBY marker
    Depositor
    Thomas Baum
    Insert
    Ubi:ccdb + RUBY
    Use
    Gateway destination
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    233306-plasmid-map-sequence-id-464366

    Enlarge

  14. Ubi:ccdb + mcherry

    Plasmid
    #233303
    Purpose
    GATEWAY compatible vector with a soybean Ubiquitin (GmUbi) promoter driving in frame epitope fusion of ccdb selection toxin and mCherry marker
    Depositor
    Thomas Baum
    Insert
    Ubi:ccdb + mcherry
    Use
    Gateway destination
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    233303-plasmid-map-sequence-id-464386

    Enlarge

  15. DEXsynth:ccdb+ RUBY

    Plasmid
    #233320
    Purpose
    GATEWAY compatible vector with a dexamethasone (DEX)-inducible promoter driving in frame epitope fusion of ccdb selection toxin and RUBY marker
    Depositor
    Thomas Baum
    Insert
    DEXsynth:ccdb+ RUBY
    Use
    Gateway destination
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    233320-plasmid-map-sequence-id-464379

    Enlarge

  16. DEXsynth:ccdb+ mcherry

    Plasmid
    #233315
    Purpose
    GATEWAY compatible vector with a dexamethasone (DEX)-inducible promoter driving in frame epitope fusion of ccdb selection toxin and mCherry marker
    Depositor
    Thomas Baum
    Insert
    DEXsynth:ccdb+ mcherry
    Use
    Gateway destination
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    233315-plasmid-map-sequence-id-464362

    Enlarge

  17. Ubi:ccdb-eGFP + RUBY

    Plasmid
    #233310
    Purpose
    GATEWAY compatible vector with a soybean Ubiquitin (GmUbi) promoter driving in frame epitope fusion of eGFP paired with ccdb selection toxin and RUBY marker
    Depositor
    Thomas Baum
    Insert
    Ubi:ccdb-eGFP + RUBY
    Use
    Gateway destination
    Tags
    eGFP
    Expression
    Mutation
    Promoter
    Available since
    March 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    233310-plasmid-map-sequence-id-464384

    Enlarge

  18. Ubi:eGFP-ccdb + RUBY

    Plasmid
    #233309
    Purpose
    GATEWAY compatible vector with a soybean Ubiquitin (GmUbi) promoter driving in frame epitope fusion of eGFP paired with ccdb selection toxin and RUBY marker
    Depositor
    Thomas Baum
    Insert
    Ubi:eGFP-ccdb + RUBY
    Use
    Gateway destination
    Tags
    eGFP
    Expression
    Mutation
    Promoter
    Available since
    March 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    233309-plasmid-map-sequence-id-464380

    Enlarge

  19. DEXsynth:ccdb-3xHA + RUBY

    Plasmid
    #233319
    Purpose
    GATEWAY compatible vector with a dexamethasone (DEX)-inducible promoter driving in frame epitope fusion of 3xHA tag paired with ccdb selection toxin and RUBY marker
    Depositor
    Thomas Baum
    Insert
    DEXsynth:ccdb-3xHA + RUBY
    Use
    Gateway destination
    Tags
    3xHA
    Expression
    Mutation
    Promoter
    Available since
    March 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    233319-plasmid-map-sequence-id-464363

    Enlarge

  20. DEXsynth:ccdb-eGFP + RUBY

    Plasmid
    #233317
    Purpose
    GATEWAY compatible vector with a dexamethasone (DEX)-inducible promoter driving in frame epitope fusion of eGFP paired with ccdb selection toxin and RUBY marker
    Depositor
    Thomas Baum
    Insert
    DEXsynth:ccdb-eGFP + RUBY
    Use
    Gateway destination
    Tags
    eGFP
    Expression
    Mutation
    Promoter
    Available since
    March 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    233317-plasmid-map-sequence-id-464455

    Enlarge

Showing: 181 - 200 of 1383 results