We narrowed to 218,434 results for: addgene

1. pMD.RVG.CVS24-B2c

   Plasmid

   #19713

   Depositor

   Manfred Schubert

   Article

   Mentis et al J Neurosci Methods. 2006 Oct 30. 157(2):

   Insert

   Rabies virus CVS24-B2c glycoprotein
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 10, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDEST-hisMBP-AsCpf1-EC

   Plasmid

   #79007

   Purpose

   Expressing his-MBP tagged AsCpf1 (E.coli codon optimized)

   Depositor

   Jin-Soo Kim

   Article

   Hur et al Nat Biotechnol. 2016 Jun 6. doi: 10.1038

   Insert

   AsCpf1
   

   Use

   Tags

   6xHis-MBP-NLS and NLS-HA

   Expression

   Bacterial

   Mutation

   Promoter

   tac promoter

   Available since

   July 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMD.RVG.CVS24-N2c

   Plasmid

   #19712

   Depositor

   Manfred Schubert

   Article

   Mentis et al J Neurosci Methods. 2006 Oct 30. 157(2):

   Insert

   Rabies virus CVS24-N2c glycoprotein
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 10, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRRL MND WASp

   Plasmid

   #36248

   Depositor

   David Rawlings

   Article

   Astrakhan et al Blood. 2012 Mar 19.

   Insert

   WASp (WAS Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   MND

   Available since

   May 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLKO-shBRCA1 #1

   Plasmid

   #44594

   Depositor

   Eros Lazzerini Denchi

   Article

   Okamoto et al Nature. 2013 Feb 6. doi: 10.1038/nature1

   Insert

   BRCA1 (Brca1 Mouse)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 7, 2013

   Availability

   Academic Institutions and Nonprofits only

6. pLKO-shBRCA1 #2

   Plasmid

   #44595

   Depositor

   Eros Lazzerini Denchi

   Article

   Okamoto et al Nature. 2013 Feb 6. doi: 10.1038/nature1

   Insert

   BRCA1 (Brca1 Mouse)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 7, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pROSA26-PA

   Plasmid

   #21271

   Depositor

   Frank Costantini

   Article

   Srinivas et al BMC Dev Biol. 2001;1:4. doi: 10.1186/147

   Insert

   ROSA26 (Gt(ROSA)26Sor Mouse)
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGH020_sgRNA_G418-GFP

   Plasmid

   #85405

   Purpose

   hu6 driven sgRNA vector with G418 and GFP selectable markers

   Depositor

   Michael Bassik

   Article

   Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

   Insert

   G418 resistance and GFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDestattB/CY (pCM327)

   Plasmid

   #68341

   Purpose

   Multisite Gateway destination vector to create transgenes for phiC31-mediated transgenesis in zebrafish, contains an attB site and alpha-cystallin:YFP (CY) transgenesis marker in backbone.

   Depositor

   Christian Mosimann, Leonard Zon

   Article

   Mosimann et al Dev Dyn. 2013 Aug;242(8):949-63. doi: 10

   Type

   Empty backbone

   Use

   Zebrafish transgenesis

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. TAL2451

    Plasmid

    #36840

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    HMGA2-TALEN_Right (Hmga2 Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Expression

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

11. TRPM2_pCSdest

    Plasmid

    #53920

    Depositor

    Roger Reeves

    Article

    Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

    Insert

    TRPM2 (TRPM2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SP6 and CMV

    Available since

    Sept. 8, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pRosa26 R1-ccdB-R2 RexNeo PI-SceI

    Plasmid

    #24418

    Depositor

    Edward Hsiao

    Article

    Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

    Insert

    
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 16, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. GFAP-Cre

    Plasmid

    #24704

    Depositor

    Michael Sofroniew

    Article

    Bush et al Neuron. 1999 Jun . 23(2):297-308.

    Insert

    Cre
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    GFAP (glial fibrillary acidic protein)

    Available since

    July 14, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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14. myc-Rictor

    Plasmid

    #1860

    Purpose

    Please see Addgene item #11367 for an updated version of this plasmid.

    Depositor

    David Sabatini

    Article

    Sarbassov et al Curr Biol 2004 Jul 27;14(14):1296-302.

    Insert

    Rictor (RICTOR Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBRα-σ70 D581G

    Plasmid

    #53732

    Purpose

    Plpp/PlacUV5- directed synthesis of the alpha NTD fused to E. coli σ70 region 4. Used as two-hybrid control. For cloning purposes, see Addgene plasmid 53734.

    Depositor

    Ann Hochschild

    Article

    Dove et al Nature. 1997 Apr 10;386(6625):627-30.

    Insert

    RNAP alpha NTD (aa 1-248) fused to E. coli sigma 70 (aa 528-613)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    The sigma 70 moiety carries the D581G substitution

    Promoter

    lpp/lacUV5 (tandem promoter)

    Available since

    June 4, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pDestattB_ubi:EGFP (pCM272)

    Plasmid

    #68339

    Purpose

    Multisite Gateway destination vector for phiC31-mediated transgenesis in zebrafish, harbors ubiquitin promoter (ubi)-driven EGFP.

    Depositor

    Christian Mosimann, Leonard Zon

    Article

    Mosimann et al Dev Dyn. 2013 Aug;242(8):949-63. doi: 10

    Insert

    EGFP
    

    Use

    Zebrafish transgenesis

    Tags

    Expression

    Mutation

    Promoter

    ubi

    Available since

    Aug. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMLM3705

    Plasmid

    #47754

    Purpose

    Expresses mammalian cell codon-optimized dCas9-VP64

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

    Insert

    codon optimized dCas9-VP64
    

    Use

    CRISPR

    Tags

    3x FLAG, NLS, and VP64

    Expression

    Mammalian

    Mutation

    D10A and H840A mutations in Cas9 (catalytically i…

    Promoter

    CMV

    Available since

    Aug. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSicoR (Puro) p65 shRNA1

    Plasmid

    #22507

    Depositor

    Tyler Jacks

    Article

    Meylan et al Nature. 2009 Nov 5;462(7269):104-7. doi:

    Insert

    p65 shRNA1 (Rela Mouse)
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. RIPK4_pCSdest

    Plasmid

    #53897

    Depositor

    Roger Reeves

    Article

    Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

    Insert

    RIPK4 (RIPK4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SP6 and CMV

    Available since

    March 27, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. Chx10BP-LCL-EGFP

    Plasmid

    #73992

    Purpose

    Cre-dependent reporter plasmid for bipolar cells in the retina

    Depositor

    Connie Cepko

    Article

    Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

    Insert

    Chx10BP-loxP-mCherry-loxP-EGFP
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Chx10BP (chx10 enhancer)

    Available since

    May 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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