We narrowed to 4,520 results for: gca

1. pColE1_sgRNA_pos6_AmpR

   Plasmid

   #119149

   Purpose

   Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR

   Depositor

   Vincent Noireaux

   Article

   Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

   Insert

   sgRNA targeting position 6 in pColE1_70a_deGFP_KanR
   

   Use

   Crispr

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. GESTALT_pX330-v1

   Plasmid

   #103061

   Purpose

   plasmid px330 with guide targeting GESTALT v1 through V5 constructs

   Depositor

   Jay Shendure

   Article

   McKenna et al Science. 2016 May 26. pii: aaf7907.

   Insert

   integration of Cas9 with guide targeting GESTALT barcodes V1 to V5
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pICSL11015

   Plasmid

   #117499

   Purpose

   FAST-Red selectable marker Golden Gate Level 1 Position 1

   Depositor

   Jonathan D Jones

   Article

   Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

   Insert

   BpiI:TGCC:pOLE1:OLE1-RFP:OLE1t:GCAA:BpiI
   

   Use

   Tags

   RFP

   Expression

   Plant

   Mutation

   Promoter

   AtOLE1

   Available since

   Oct. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLKO.1 puro-humanU6-shRNA FASN

   Plasmid

   #82327

   Purpose

   used to silence target human FASN expression via RNA interference (3rd gen lentiviral vector)

   Depositor

   Elizabeth Stoll

   Insert

   shRNA to target Fatty Acid Synthase (FASN) (FASN Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. lenti-CHMP4B gRNA1

   Plasmid

   #211628

   Purpose

   sgRNA-1 against CHMP4B

   Depositor

   Agnel Sfeir

   Article

   Brambati et al Science. 2023 Aug 11;381(6658):653-660.

   Insert

   sgRNA CHMP4B
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   March 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. gRNA-eGFP-Reporter

   Plasmid

   #60719

   Purpose

   Expresses guide RNA used to activate pGL3-Basic-8x-gRNA-eGFP reporter.

   Depositor

   Charles Gersbach

   Article

   Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

   Insert

   gRNA (5'-AAAGGTCGAGAAACTGCAAA-3')
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and mouse U6

   Available since

   Jan. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. ZO-1 shRNA 3253

   Plasmid

   #37206

   Depositor

   James Anderson, Alan Fanning

   Article

   Van Itallie et al Mol Biol Cell. 2009 Sep;20(17):3930-40.

   Insert

   dog ZO-1 shRNA (TJP1 canine)
   

   Use

   RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 27, 2012

   Availability

   Academic Institutions and Nonprofits only

8. ZO-1 shRNA 2968

   Plasmid

   #37208

   Depositor

   James Anderson, Alan Fanning

   Article

   Van Itallie et al Mol Biol Cell. 2009 Sep;20(17):3930-40.

   Insert

   dog ZO-1 shRNA (TJP1 canine)
   

   Use

   RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 27, 2012

   Availability

   Academic Institutions and Nonprofits only

9. PX458_CEBPB_2

   Plasmid

   #64047

   Purpose

   Expresses gRNA against human CEBPB along with Cas9 with 2A GFP

   Depositor

   Richard M. Myers, Eric Mendenhall

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Insert

   gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. gRNA-POLQ-Halo

    Plasmid

    #211636

    Purpose

    sgRNA against POLQ

    Depositor

    Agnel Sfeir

    Article

    Brambati et al Science. 2023 Aug 11;381(6658):653-660.

    Insert

    sgRNA POLQ
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. ACE2 g3

    Plasmid

    #153013

    Purpose

    A guide RNA targeting ACE2 in a lentiviral plasmid co-expressing mCherry

    Depositor

    Jason Sheltzer

    Insert

    ACE2 (ACE2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Available since

    June 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR BFP-guide1

    Plasmid

    #118157

    Purpose

    CRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA1 against the BFP CDS

    Depositor

    Jorge Ferrer

    Insert

    KRAB-dCas9-P2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a core and U6

    Available since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJF131B

    Plasmid

    #113326

    Purpose

    pTet-W108 scRNA.b2, pTet-RR2-sgRNA

    Depositor

    Jesse Zalatan

    Article

    Dong et al Nat Commun. 2018 Jun 27;9(1):2489. doi:

    Insert

    W108 scRNA_MS2 and sgRNA_RR2
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBbS5c-AcrB/AcrD/AcrFa, ABO_0964

    Plasmid

    #86206

    Purpose

    Expresses efflux pump in E. coli to provide resistance to biofuel (low copy)

    Depositor

    Mary Dunlop

    Article

    Turner et al ACS Synth Biol. 2015 Oct 16;4(10):1056-6

    Insert

    Efflux pump
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    ATCGTTTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATAA…

    Available since

    March 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLenti-Crispr-V2-HMID.v1

    Plasmid

    #103062

    Purpose

    LentiCrisprV2 with guide targeting V1-V5 GESTALT barcodes

    Depositor

    Jay Shendure

    Article

    McKenna et al Science. 2016 May 26. pii: aaf7907.

    Insert

    Cas9 plus expressed GESTALT V1-V5 guide
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pmCherry-U6-RNF103-CHMP3

    Plasmid

    #140584

    Purpose

    Expresses a gRNA and mCherry in mammalian cells

    Depositor

    Feng Zhang

    Article

    Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

    Insert

    gRNA RNF103-CHMP3
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pColE1_sgRNA_pos7_AmpR

    Plasmid

    #119150

    Purpose

    Encodes sgRNA targeting position 7 in pColE1_70a_deGFP_KanR

    Depositor

    Vincent Noireaux

    Article

    Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

    Insert

    sgRNA targeting position 7 in pColE1_70a_deGFP_KanR
    

    Use

    Crispr

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pColE1_sgRNA_pos5_AmpR

    Plasmid

    #119148

    Purpose

    Encodes sgRNA targeting position 5 in pColE1_70a_deGFP_KanR

    Depositor

    Vincent Noireaux

    Article

    Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

    Insert

    sgRNA targeting position 5 in pColE1_70a_deGFP_KanR
    

    Use

    Crispr

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. LRT2B-GO

    Plasmid

    #136908

    Purpose

    Lentivirus expressing GO sgRNA

    Depositor

    Lukas Dow

    Article

    Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

    Insert

    sgGO
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    hU6

    Available since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pX330.LoxP

    Plasmid

    #66586

    Purpose

    sgRNA for LSL

    Depositor

    Wen Xue

    Article

    Li et al Genome Biol. 2015 May 28;16(1):111.

    Insert

    LoxP sgRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 11, 2015

    Availability

    Academic Institutions and Nonprofits only