We narrowed to 17,354 results for: She

1. pTUB:FLuc

   Plasmid

   #114578

   Purpose

   Constitutive expression of firefly luciferase in T. gondii using the alpha tubulin promoter; floxed pyrimethamine selectable marker; UPRT locus flanking sequence to direct integration

   Depositor

   David Sibley

   Insert

   Firefly Luciferase
   

   Use

   Cre/Lox and Luciferase

   Tags

   Expression

   Mutation

   Promoter

   alpha tubulin T. gondii

   Available since

   Aug. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. mEmerlad-MCPB2-C-18

   Plasmid

   #62767

   Purpose

   Localization: Actin Capping Protein , C1 Cloning Vector, Excitation:487, Emission:509

   Depositor

   Michael Davidson

   Insert

   MCPB2
   

   Use

   Tags

   mEmerald

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available since

   March 10, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pJK694

   Plasmid

   #72379

   Purpose

   Produces human phosphoribosyl pyrophosphate amidotransferase (PRAT), synthetic gene

   Depositor

   T. J. Kappock

   Insert

   phosphoribosyl pyrophosphate amidotransferase (PPAT Human)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GPD

   Available since

   Feb. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

4. pHEX2 T494 TALENs

   Plasmid

   #44464

   Depositor

   Ross Johnson, Roger Hellens

   Insert

   AtCRU3 T494 TALENs
   

   Use

   Plant transformation

   Tags

   Expression

   Mutation

   Promoter

   CaMV 35S

   Available since

   May 6, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pFA6a-SRM (sortase recognition motif)-hphMX4

   Plasmid

   #54488

   Purpose

   PCR based C-terminal SRM tagging in yeast

   Depositor

   Eri Sakata

   Insert

   SRM (sortase-recognition motif)
   

   Use

   Yeast genome targeting

   Tags

   hphMX4

   Expression

   Mutation

   Promoter

   Available since

   Aug. 28, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pKPY738

   Plasmid

   #72674

   Purpose

   C. elegans let-858 3' UTR (Gateway Entry Clone)

   Depositor

   David Tirrell

   Insert

   let-858 3' UTR [From pPD129.57 (+2554 to +2960)] in pDONR P2R-P3
   

   Use

   Gateway entry clone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. mGeos2-VEL-C1

   Plasmid

   #54541

   Purpose

   Localization: C1 Cloning Vector, Excitation: 503 / 569, Emission: 514 / 581

   Depositor

   Michael Davidson

   Type

   Empty backbone

   Use

   Tags

   mGeos2-VEL

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 2, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

8. pFA6a-kanMX-tetO-Pcyc1-GST-LINKER

   Plasmid

   #52688

   Purpose

   Same as 41026, with additional linker

   Depositor

   Michael Nick Boddy

   Insert

   tetO7-Pcyc1-GST+LINKER
   

   Use

   Promoter replacement

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 30, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMC-ME_BFP-P2A-NTRv2-pA

   Plasmid

   #200538

   Purpose

   mini circle middle entry plasmid for BFP-P2A-NTR version 2-pA with flanking BsaI cut sites

   Depositor

   Junsu Kang

   Insert

   BFP, NTRv2
   

   Use

   Mini-golden

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMC-ME_2a-mCherry-pA ORF-1 with ATG

    Plasmid

    #200522

    Purpose

    mini circle middle entry plasmid for 2a-mCherry-pA ORF 1 (with ATG) with flanking BsaI cut sites

    Depositor

    Junsu Kang

    Insert

    mCherry
    

    Use

    Mini-golden

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMC-ME_2a-mCherry-pA ORF-2 with ATG

    Plasmid

    #200523

    Purpose

    mini circle middle entry plasmid for 2a-mCherry-pA ORF 2 (with ATG) with flanking BsaI cut sites

    Depositor

    Junsu Kang

    Insert

    mCherry
    

    Use

    Mini-golden

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMC-ME_2a-mCherry-pA ORF-3 with ATG

    Plasmid

    #200524

    Purpose

    mini circle middle entry plasmid for 2a-mCherry-pA ORF 3 (with ATG) with flanking BsaI cut sites

    Depositor

    Junsu Kang

    Insert

    mCherry
    

    Use

    Mini-golden

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMC-ME mCherry-P2A-NTRv2-pA

    Plasmid

    #200532

    Purpose

    mini circle middle entry plasmid for mCherry- P2A- ntr version 2- pA with flanking BsaI cut sites

    Depositor

    Junsu Kang

    Insert

    mCherry, NTRv2
    

    Use

    Mini-golden

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMC-ME_2a-EGFP-pA ORF-1 with ATG

    Plasmid

    #200514

    Purpose

    mini circle middle entry plasmid for 2a-eGFP-pA ORF1 (with ATG) with flanking BsaI cut sites

    Depositor

    Junsu Kang

    Insert

    eGFP
    

    Use

    Mini-golden

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMC-ME_2a-EGFP-pA ORF-2 with ATG

    Plasmid

    #200515

    Purpose

    mini circle middle entry plasmid for 2a-GFP-pA ORF 2 (with ATG) with flanking BsaI cut sites

    Depositor

    Junsu Kang

    Insert

    eGFP
    

    Use

    Mini-golden

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMC-ME_2a-EGFP-pA ORF-3 with ATG

    Plasmid

    #200516

    Purpose

    mini circle middle entry plasmid for 2a-GFP-pA-ORF 3 (with ATG) with flanking BsaI cut sites

    Depositor

    Junsu Kang

    Insert

    eGFP
    

    Use

    Mini-golden

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pU6:3-tevopreq1

    Plasmid

    #190909

    Purpose

    Expression of single epegRNA under control of Drosophila U6:3 promoter. Can be used to generate transgenic flies with vermillion+ selection.

    Depositor

    Norbert Perrimon

    Insert

    Empty Backbone
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    July 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. Ec holD-pET(3c)

    Plasmid

    #237236

    Purpose

    over-express E. coli holD (Pol III psi subunit)

    Depositor

    Michael O'Donnell

    Insert

    holD (psi) (holD E. coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Ec holC-pET(3c)

    Plasmid

    #237235

    Purpose

    over-express E. coli holC (Pol III chi subunit)

    Depositor

    Michael O'Donnell

    Insert

    holC (chi) (holC E. coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAGGS-MTS(S23A)-EGFP-V5

    Plasmid

    #200929

    Purpose

    expresses MTS(S23A)-EGFP-V5 in mammalian cells

    Depositor

    Hung-Chun Chang

    Insert

    EGFP
    

    Use

    Tags

    V5 and mitochondrial targeting sequence (MTS)

    Expression

    Mammalian

    Mutation

    MTS amino acid 23 mutated to Alanine

    Promoter

    CAG

    Available since

    April 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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21. pCAGGS-MTS(S6A)-EGFP-V5

    Plasmid

    #200817

    Purpose

    expresses MTS(S6A)-EGFP-V5 in mammalian cells

    Depositor

    Hung-Chun Chang

    Insert

    EGFP
    

    Use

    Tags

    V5 and mitochondrial targeting sequence (MTS)

    Expression

    Mammalian

    Mutation

    Changed Serine 6 to Alanine in MTS

    Promoter

    CAG

    Available since

    April 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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22. YFP Rab11 Q70L in pcDNA3.1

    Plasmid

    #90467

    Purpose

    RhoA mediated cell migration

    Depositor

    Narasimhan Gautam

    Insert

    Rab11 Q70L
    

    Use

    Tags

    mEYFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCCI-YFVΔCMENS1-Flpo

    Plasmid

    #233592

    Purpose

    pCCI vector encoding YFV-17D lacking C, prM, E and NS1 proteins but with Flpo

    Depositor

    Wei Xu

    Insert

    YFV-17D lacking C, prM, E and NS1 proteins but with Flpo
    

    Use

    Transneural tracing

    Tags

    Expression

    Mutation

    Lacking C, prM, E and NS1

    Promoter

    SP6

    Available since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET-Impact-T4gp41

    Plasmid

    #228207

    Purpose

    Expression in BL21(DE3) of T4 helicase

    Depositor

    Stephen Benkovic

    Insert

    41 (41 Escherichia phage T4)
    

    Use

    Tags

    Sce VMA intein-chitin binding domain

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET-Impact-T4gp59

    Plasmid

    #228206

    Purpose

    Expression in BL21(DE3) of WT T4 helicase loader

    Depositor

    Stephen Benkovic

    Insert

    59 (59 Escherichia phage T4)
    

    Use

    Tags

    Sce VMA intein-chitin binding domain

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. Pv6PGDH-pNic-Bsa4

    Plasmid

    #231102

    Purpose

    Expresses Plasmodium vivax 6-phosphogluconate dehydrogeanse under T7 with HIS tag

    Depositor

    Josh Beckham

    Insert

    6-phosphogluconate dehydrogenase
    

    Use

    Tags

    HIS tag

    Expression

    Mutation

    Promoter

    Available since

    Feb. 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. 1203D-5'dDR-mCh(22)-GFP-bGHpolyA

    Plasmid

    #221534

    Purpose

    EGFP reporter construct with double unprocessed direct repeat (35nt) at 5' end, 22nt mCherry-targeting spacer in between DRs

    Depositor

    Omar Akbari

    Insert

    eGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. 1203E-3'dDR-mCh(22)-GFP-bGHpolyA

    Plasmid

    #221535

    Purpose

    EGFP reporter construct with double unprocessed direct repeat (35nt) at 3' end, 22nt mCherry-targeting spacer in between DRs

    Depositor

    Omar Akbari

    Insert

    eGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. 1203F-5’+3'dDR-mCh(22)-GFP-bGHpolyA

    Plasmid

    #221536

    Purpose

    EGFP reporter construct with double unprocessed direct repeat (35nt) at 5' and 3' end, 22nt mCherry-targeting spacer in between DRs

    Depositor

    Omar Akbari

    Insert

    eGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. 1203AB-CMV-Cas7.1-H43A-mutant

    Plasmid

    #221541

    Purpose

    Effector expresses Cas7.1 (truncated from DiCas7-11), H to A mutation at amino acid 43

    Depositor

    Omar Akbari

    Insert

    Truncated DiCas7-11
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. 1203AC-CMV-Cas7.1-Y55A-mutant

    Plasmid

    #221542

    Purpose

    Effector expresses Cas7.1 domain (truncated from DiCas7-11), Y to A mutation at amino acid 55

    Depositor

    Omar Akbari

    Insert

    Truncated DiCas7-11
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pGLO-Yellow-AZA

    Plasmid

    #220783

    Purpose

    Expresses a yellow chromoprotein (visible light and fluorescence) with a his-tag and an inducible GFP (araBAD-promoter)

    Depositor

    Angela Leu

    Insert

    yellow chromoprotein
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. MZB205

    Plasmid

    #228490

    Purpose

    Empty 10xHis-SUMO (Smt3) fused Avi-tag, HRV-3C cleavable vector for rapid cloning/DNA assembly via SwaI restriction site for E. coli based protein expression

    Depositor

    Richard Baker

    Type

    Empty backbone

    Use

    Tags

    10xHis-SUMO(Smt3)-Avitag-HRV

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pDual_dsCas9_Venus

    Plasmid

    #214678

    Purpose

    Lentiviral expression vector for an inducible Cas9-P2A-Venus with two empty sgRNA cloning sites

    Depositor

    Kevin Janes

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pMDW133

    Plasmid

    #227248

    Purpose

    Lambda DNA for torsional templates

    Depositor

    Michelle Wang

    Insert

    Lambda DNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Plac

    Available since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. 2xUAS-1xhsp70-attB

    Plasmid

    #220317

    Purpose

    includes 2 UAS cassettes and 1 heat-shock inducible hsp70 casette

    Depositor

    Erdem Bangi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pET29a-DDX53

    Plasmid

    #222205

    Purpose

    Express DDX53 protein in bacteria

    Depositor

    Yuliang Wu

    Insert

    DDX53 (DDX53 Human)
    

    Use

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pET41-FeoB-C675S

    Plasmid

    #216756

    Purpose

    Investigation of the potential interaction of the FeoB Cys675 residue with ferrous iron

    Depositor

    Rietie Venter

    Insert

    feoB (PA4358 P. aeruginosa (Bacteria))
    

    Use

    Tags

    8x His

    Expression

    Bacterial

    Mutation

    Amino acid C675 is substituted with S.

    Promoter

    Available since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pET41-FeoB-C429S

    Plasmid

    #216755

    Purpose

    Investigation of the potential interaction of the FeoB Cys429 residue with ferrous iron

    Depositor

    Rietie Venter

    Insert

    feoB (PA4358 P. aeruginosa (Bacteria))
    

    Use

    Tags

    8x His

    Expression

    Bacterial

    Mutation

    Amino acid C429 is substituted with S.

    Promoter

    Available since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pET41-FeoB-D123N

    Plasmid

    #216754

    Purpose

    Production of a FeoB that is unable to bind GTP for a negative control in GTPases assays

    Depositor

    Rietie Venter

    Insert

    feoB (PA4358 P. aeruginosa (Bacteria))
    

    Use

    Tags

    8x His

    Expression

    Bacterial

    Mutation

    Amino acid D123 is substituted with N.

    Promoter

    Available since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge