We narrowed to 4,342 results for: Pam

1. pMXS-IRES-BLAST flag-CAD delta Loop1

   Plasmid

   #169831

   Purpose

   Expresses C-terminal flag-tagged CAD with deletion of amino acids F1366 - P1376 in allosteric domain

   Depositor

   Richard Possemato

   Article

   Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

   Insert

   CAD (CAD Human)
   

   Use

   Retroviral

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   Deletion of amino acids F1366 - P1376; TCCC ->…

   Available Since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMXS-IRES-BLAST flag-CAD delta Loop2

   Plasmid

   #169832

   Purpose

   Expresses C-terminal flag-tagged CAD with deletion of amino acids R1398 - S1407 in allosteric domain

   Depositor

   Richard Possemato

   Article

   Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

   Insert

   CAD (CAD Human)
   

   Use

   Retroviral

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   Deletion of amino acids R1398 - S1407; TCCC ->…

   Available Since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMXS-IRES-BLAST flag-CAD S1406A

   Plasmid

   #169820

   Purpose

   Expresses C-terminal flag-tagged CAD with mutation of reported PKA phosphorylation site

   Depositor

   Richard Possemato

   Article

   Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

   Insert

   CAD (CAD Human)
   

   Use

   Retroviral

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   S1406A; TCCC -> AGTC silent mutations at nt527…

   Available Since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMXS-IRES-BLAST flag-CAD S1859E

   Plasmid

   #169822

   Purpose

   Expresses C-terminal flag-tagged CAD with mutation of reported S6K phosphorylation site

   Depositor

   Richard Possemato

   Article

   Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

   Insert

   CAD (CAD Human)
   

   Use

   Retroviral

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   S1859E; TCCC -> AGTC silent mutations at nt527…

   Available Since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMXS-IRES-BLAST flag-CAD T456A S1406A

   Plasmid

   #169823

   Purpose

   Expresses C-terminal flag-tagged CAD with mutation of reported Erk1/2 and PKA phosphorylation site

   Depositor

   Richard Possemato

   Article

   Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

   Insert

   CAD (CAD Human)
   

   Use

   Retroviral

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   T456A S1406A; TCCC -> AGTC silent mutations at…

   Available Since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMXS-IRES-BLAST flag-CAD T456A S1859A

   Plasmid

   #169824

   Purpose

   Expresses C-terminal flag-tagged CAD with mutation of reported Erk1/2 and S6K phosphorylation site

   Depositor

   Richard Possemato

   Article

   Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

   Insert

   CAD (CAD Human)
   

   Use

   Retroviral

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   T456A S1859A; TCCC -> AGTC silent mutations at…

   Available Since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMXS-IRES-BLAST flag-CAD S1406A

   Plasmid

   #169820

   Purpose

   Expresses C-terminal flag-tagged CAD with mutation of reported PKA phosphorylation site

   Depositor

   Richard Possemato

   Article

   Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

   Insert

   CAD (CAD Human)
   

   Use

   Retroviral

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   S1406A; TCCC -> AGTC silent mutations at nt527…

   Available Since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMXS-IRES-BLAST flag-CAD S1859E

   Plasmid

   #169822

   Purpose

   Expresses C-terminal flag-tagged CAD with mutation of reported S6K phosphorylation site

   Depositor

   Richard Possemato

   Article

   Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

   Insert

   CAD (CAD Human)
   

   Use

   Retroviral

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   S1859E; TCCC -> AGTC silent mutations at nt527…

   Available Since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMXS-IRES-BLAST flag-CAD T456A S1406A

   Plasmid

   #169823

   Purpose

   Expresses C-terminal flag-tagged CAD with mutation of reported Erk1/2 and PKA phosphorylation site

   Depositor

   Richard Possemato

   Article

   Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

   Insert

   CAD (CAD Human)
   

   Use

   Retroviral

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   T456A S1406A; TCCC -> AGTC silent mutations at…

   Available Since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMXS-IRES-BLAST flag-CAD T456A S1859A

    Plasmid

    #169824

    Purpose

    Expresses C-terminal flag-tagged CAD with mutation of reported Erk1/2 and S6K phosphorylation site

    Depositor

    Richard Possemato

    Article

    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

    Insert

    CAD (CAD Human)
    

    Use

    Retroviral

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    T456A S1859A; TCCC -> AGTC silent mutations at…

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSCBE3-NG-HF1

    Plasmid

    #218160

    Purpose

    This plasmid harbors the base editor SCBE3-NG-HF1 along with an sgRNA cloning cassette, facilitating high-fidelity cytosine base editing at targets bearing NG PAM in Streptomyces.

    Depositor

    Yuhui Sun

    Article

    Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:

    Inserts

    scbe3-NG-HF1
    a programmable sgRNA cloning cassette
    

    Use

    CRISPR; Sgrna transcription

    Expression

    Bacterial

    Mutation

    Mutations in the portion of SpCas9 : D10A / N497A…

    Promoter

    rpsL promoter

    Available Since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSCBE3-NG-HF1

    Plasmid

    #218160

    Purpose

    This plasmid harbors the base editor SCBE3-NG-HF1 along with an sgRNA cloning cassette, facilitating high-fidelity cytosine base editing at targets bearing NG PAM in Streptomyces.

    Depositor

    Yuhui Sun

    Article

    Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:

    Inserts

    scbe3-NG-HF1
    a programmable sgRNA cloning cassette
    

    Use

    CRISPR; Sgrna transcription

    Expression

    Bacterial

    Mutation

    Mutations in the portion of SpCas9 : D10A / N497A…

    Promoter

    rpsL promoter

    Available Since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSCBE3-NG-Hypa

    Plasmid

    #218161

    Purpose

    This plasmid harbors the base editor SCBE3-NG-Hypa along with an sgRNA cloning cassette, facilitating high-fidelity cytosine base editing at targets bearing NG PAM in Streptomyces.

    Depositor

    Yuhui Sun

    Article

    Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:

    Inserts

    scbe3-NG-Hypa
    a programmable sgRNA cloning cassette
    

    Use

    CRISPR; Sgrna transcription

    Expression

    Bacterial

    Mutation

    Mutations in the portion of SpCas9 : D10A / N692A…

    Promoter

    rpsL promoter

    Available Since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSCBE3-NG-Hypa

    Plasmid

    #218161

    Purpose

    This plasmid harbors the base editor SCBE3-NG-Hypa along with an sgRNA cloning cassette, facilitating high-fidelity cytosine base editing at targets bearing NG PAM in Streptomyces.

    Depositor

    Yuhui Sun

    Article

    Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:

    Inserts

    scbe3-NG-Hypa
    a programmable sgRNA cloning cassette
    

    Use

    CRISPR; Sgrna transcription

    Expression

    Bacterial

    Mutation

    Mutations in the portion of SpCas9 : D10A / N692A…

    Promoter

    rpsL promoter

    Available Since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSCBE3-NG-HF1-Hypa

    Plasmid

    #218162

    Purpose

    This plasmid harbors the base editor SCBE3-NG-HF1-Hypa along with an sgRNA cloning cassette, facilitating high-fidelity cytosine base editing at targets bearing NG PAM in Streptomyces.

    Depositor

    Yuhui Sun

    Article

    Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:

    Inserts

    scbe3-NG-HF1-Hypa
    a programmable sgRNA cloning cassette
    

    Use

    CRISPR; Sgrna transcription

    Expression

    Bacterial

    Mutation

    Mutations in the portion of SpCas9 : D10A / N497A…

    Promoter

    rpsL promoter

    Available Since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSCBE3-NG-HF1-Hypa

    Plasmid

    #218162

    Purpose

    This plasmid harbors the base editor SCBE3-NG-HF1-Hypa along with an sgRNA cloning cassette, facilitating high-fidelity cytosine base editing at targets bearing NG PAM in Streptomyces.

    Depositor

    Yuhui Sun

    Article

    Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:

    Inserts

    scbe3-NG-HF1-Hypa
    a programmable sgRNA cloning cassette
    

    Use

    CRISPR; Sgrna transcription

    Expression

    Bacterial

    Mutation

    Mutations in the portion of SpCas9 : D10A / N497A…

    Promoter

    rpsL promoter

    Available Since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDonor-Prf1-ires-mCherry

    Plasmid

    #209073

    Purpose

    Targeting vector for the mouse Prf1 locus to correct prf1 gene

    Depositor

    Klaus Rajewsky

    Article

    Li et al Sci Immunol. 2024 Feb 2;9(92):eadi0042.

    Insert

    Prf1-ires-mCherry HDR template (parts of Prf1 with mutated PAM) (Prf1 Mouse)
    

    Use

    CRISPR

    Available Since

    March 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDonor-Prf1-mock-ires-mCherry

    Plasmid

    #209074

    Purpose

    Targeting vector for the mouse Prf1 locus to correct prf1 gene

    Depositor

    Klaus Rajewsky

    Article

    Li et al Sci Immunol. 2024 Feb 2;9(92):eadi0042.

    Insert

    Prf1-mock-ires-mCherry HDR template (parts of Prf1(S399Stop) with mutated PAM) (Prf1 Mouse)
    

    Use

    CRISPR

    Available Since

    March 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDonor-Prf1-ires-mCherry

    Plasmid

    #209073

    Purpose

    Targeting vector for the mouse Prf1 locus to correct prf1 gene

    Depositor

    Klaus Rajewsky

    Article

    Li et al Sci Immunol. 2024 Feb 2;9(92):eadi0042.

    Insert

    Prf1-ires-mCherry HDR template (parts of Prf1 with mutated PAM) (Prf1 Mouse)
    

    Use

    CRISPR

    Available Since

    March 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDonor-Prf1-mock-ires-mCherry

    Plasmid

    #209074

    Purpose

    Targeting vector for the mouse Prf1 locus to correct prf1 gene

    Depositor

    Klaus Rajewsky

    Article

    Li et al Sci Immunol. 2024 Feb 2;9(92):eadi0042.

    Insert

    Prf1-mock-ires-mCherry HDR template (parts of Prf1(S399Stop) with mutated PAM) (Prf1 Mouse)
    

    Use

    CRISPR

    Available Since

    March 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge