We narrowed to 10,498 results for: aga

1. pMG058 MBP-GS 634-666-His

   Plasmid

   #154077

   Purpose

   Expresses MBP-GS 634-666-His (human 33aa Glycogen Synthase peptide as a fusion protein with MBP and His- tags) in E.coli

   Depositor

   Jesse Zalatan

   Article

   Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

   Insert

   GYS1 (GYS1 Human)
   

   Use

   Tags

   His6 and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Tac

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. OSR1 gRNA (BRDN0001146927)

   Plasmid

   #76147

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human OSR1

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   OSR1 (OSR1, OXSR1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. PRKD1 gRNA (BRDN0001146537)

   Plasmid

   #77228

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PRKD1

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PRKD1 (PRKD1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPICZ-mouseGIRK2R201A-eGFP-StII-6xHis

   Plasmid

   #124278

   Purpose

   Expresses mouse GIRK2 R201A as a TEV protease cleavable C-terminal fusion to eGFP, strep tag II, and 6x His tag

   Depositor

   Arthur Laganowsky

   Article

   Liu et al Nat Commun. 2019 Mar 22;10(1):1352. doi:

   Insert

   GIRK2 (Kcnj6 Mouse)
   

   Use

   Tags

   eGFP-StreptagII-6xHis

   Expression

   Yeast

   Mutation

   R201A

   Promoter

   AOX1

   Available since

   April 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLCHKO_TK1_HPRT1_Lb_1

   Plasmid

   #155059

   Purpose

   Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA targeting TK1 using SpCas9 and HPRT1 using LbCas12a (CHyMErA system)

   Depositor

   Benjamin Blencowe, Jason Moffat

   Article

   Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

   Insert

   (hg)RNA targeting TK1 using SpCas9 and HPRT1 using LbCas12a
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEX-6P-1-INTS3-C

   Plasmid

   #128417

   Purpose

   Express INTS3 C-termini (514-1042 aa) in E. coli with a GST tag (N-terminal)

   Depositor

   Yuliang Wu

   Article

   Vidhyasagar et al Biochem J. 2018 Jan 2;475(1):45-60. doi:

   Insert

   INTS3 (INTS3 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   INTS3 C-terminal region (514-1042 aa)

   Promoter

   Tac promoter

   Available since

   Aug. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKTop::tse5-CT (1169-1281)

   Plasmid

   #192955

   Purpose

   For membrane insertion study; Expresses tse5-CT (1169-1281) /PhoA (22-472)/LacZ (4-60)fusion with PelB signalpeptide fused at the N-terminus

   Depositor

   David Albesa-Jové

   Article

   Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

   Insert

   tse5-CT (1169-1281)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   encodes for tse5 (1169-1281)

   Promoter

   Available since

   Dec. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLCHKO_MDM4_exon_deletion_3

   Plasmid

   #155075

   Purpose

   Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of MDM4 exon using SpCas9 and LbCas12a nucleases (CHyMErA system)

   Depositor

   Benjamin Blencowe, Jason Moffat

   Article

   Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

   Insert

   (hg)RNA for deletion of MDM4 exon using SpCas9 and LbCas12a nucleases
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV_Donor/Reporter-COL4A5

   Plasmid

   #130280

   Purpose

   COL4A5 mutation-specific sgRNA under the control of U6 promoter; mCherry-sgRNA target-(out of frame)EGFP expression cassette; COL4A5 donor template

   Depositor

   Silvestro Conticello

   Article

   Daga et al Eur J Hum Genet. 2019 Nov 21. pii: 10.10

   Insert

   mCherry-eGFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Dec. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. plko-tet-on-puromycin-shTRX1-259

    Plasmid

    #110920

    Purpose

    To knockdown Thioredoxin-1 following doxycycline addition

    Depositor

    Priyamvada Rai

    Article

    Samaranayake et al Nat Commun. 2017 Oct 31;8(1):1204. doi:

    Insert

    TRX1 (TXN Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    unknown

    Available since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti-CMV-cGAS R71/75E C396/7A-HA

    Plasmid

    #130920

    Purpose

    Expresses human cGAS with R71/75E and C396/397A point mutations; Puromycin selection marker

    Depositor

    Jonathan Kagan

    Article

    Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

    Insert

    human cGAS R71/75E C396/7A
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    aa 71 and 75 mutated to glutamate; aa 396 and 397…

    Promoter

    CMV

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLINK cyclin T1 (1-280) myc (all 200) (P#575)

    Plasmid

    #14610

    Depositor

    Matija Peterlin

    Article

    Fujinaga et al Proc Natl Acad Sci U S A. 1999 Feb 16. 9

    Insert

    cyclin T1 (1-280) (all 200) (CCNT1 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    C198A, C200A, H202A, C205A. Active in 3T3 cells.

    Promoter

    Available since

    June 30, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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13. PX-TIR-GFP

    Plasmid

    #52737

    Purpose

    Retroviral vector expresses the lipid binding domain of p40-phox fused to the TIR of hTIRAP and GFP. Also contains IRES-hCD2 as selectable marker

    Depositor

    Jonathan Kagan

    Article

    Bonham et al Cell. 2014 Feb 13;156(4):705-16. doi: 10

    Insert

    Toll/IL-1 Receptor adaptor protein (TIRAP Human)
    

    Use

    Retroviral

    Tags

    GFP and p40 Phox lipid binding domain

    Expression

    Mammalian

    Mutation

    Deleted amino acids 1-85

    Promoter

    Available since

    June 4, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA3.1 2xFLAG-2xSTREP EMI1

    Plasmid

    #158947

    Purpose

    Express human EMI1 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Marzio et al Mol Cell. 2019 Jan 17;73(2):224-237.e6.

    Insert

    EMI1 (FBXO5 Human)
    

    Use

    Tags

    2X FLAG 2X STREP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. NFATc2-CRISPR-Nick 1/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

    Plasmid

    #75234

    Purpose

    CRISPR/Cas9 NICKASE plasmid against human NFATc2 (1/2)

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    sgRNA against NFATc2 (NFATC2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. TEC gRNA (BRDN0001148069)

    Plasmid

    #77024

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TEC

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    TEC (TEC, TEC Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. YES1 gRNA (BRDN0001145317)

    Plasmid

    #77965

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human YES1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    YES1 (YES1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3.1 2xFLAG-2xSTREP-Keap1 (mouse)

    Plasmid

    #236421

    Purpose

    transient overexpression of KEAP1 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Marzio et al Cell. 2022 Jan 6;185(1):169-183.e19. doi

    Insert

    KEAP1 (Keap1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1 2xFLAG-2xSTREP-KEAP1 (human)

    Plasmid

    #236422

    Purpose

    transient overexpression of KEAP1 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Marzio et al Cell. 2022 Jan 6;185(1):169-183.e19. doi

    Insert

    KEAP1 (KEAP1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET-30a-NSP14-SARS-CoV-2

    Plasmid

    #236423

    Purpose

    bacterial protein expression of NSP14

    Depositor

    Michele Pagano

    Article

    Rona et al Cell Death Differ. 2022 Feb;29(2):285-29

    Insert

    NSP14 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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