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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 2,603 results for: pCas

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Showing: 2001 - 2020 of 2603 results

  1. HUHe variant click editor (CE1) - pCMV-T7-GuCV-nCas9-EcKlenow (JO1279)

    Plasmid
    #217788
    Purpose
    Variant CE1 construct with GuCV HUH endonuclease, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    GuCV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)
    Promoter
    CMV and T7
    Available Since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217788-plasmid-map-sequence-id-432851

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  2. HUHe variant click editor (CE1) - pCMV-T7-PCV1-nCas9-EcKlenow (JO1315)

    Plasmid
    #217789
    Purpose
    Variant CE1 construct with PCV1 HUH endonuclease, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    PCV1-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)
    Promoter
    CMV and T7
    Available Since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217789-plasmid-map-sequence-id-432852

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  3. Click editor utilizing Cdc13(Y556A) for template recruitment - pCMV-T7-Cdc13(Y556A)-nCas9-EcKlenow (JO1329)

    Plasmid
    #217797
    Purpose
    Variant CE1 construct with Cdc13(Y556A) telomere binding protein for template recruitment, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    Cdc13(Y556A)-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    Cdc13(Y556A); nSpCas9(H840A); EcKlenow(-exo;D355A…
    Promoter
    CMV and T7
    Available Since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217797-plasmid-map-sequence-id-432864

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  4. Click editor utilizing mSA for template recruitment and ePhi29 DNA polymerase - pCMV-T7-mSA-nCas9-ePhi29 (JO1398)

    Plasmid
    #217803
    Purpose
    Variant CE1 construct with mSA for template recruitment and ePhi29(D169A) DNA pol, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    mSA-linker-SV40NLS-nSpCas9-BPNLS-ePhi29-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); Phi29(D169A/M8R/V51A/M97T/G197D/E…
    Promoter
    CMV and T7
    Available Since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217803-plasmid-map-sequence-id-432890

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  5. Cas9 ROSA sgRNA mCherry

    Plasmid
    #155280
    Purpose
    Lentiviral Cas9 expression, ROSA sgRNA expression, mCherry marker
    Depositor
    Martin Carroll
    Insert
    spCas9
    Available Since
    Aug. 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    155280-plasmid-map-sequence-id-309987

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  6. pMPO 58

    Plasmid
    #68362
    Purpose
    Expression vectors for the salycilate cascade expression system (Pnah/NahR-Psal/XylS2).ApR, expression vector with rrnBT1T2-Pm- nasF attenuator-T7 SD sequence-MCSII, ColE1 replication origin
    Depositor
    Eduardo Santero
    Article
    Medina et al PLoS One. 2011;6(8):e23055. doi: 10.1371
    Type
    Empty backbone
    Expression
    Bacterial
    Available Since
    Nov. 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    68362-plasmid-map-sequence-id-128759

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  7. pMPO 57

    Plasmid
    #68361
    Purpose
    Expression vectors for the salycilate cascade expression system (Pnah/NahR-Psal/XylS2).ApR, expression vector with rrnBT1T2-Pm-nasF attenuator-MCSII, ColE1 replication origin
    Depositor
    Eduardo Santero
    Article
    Medina et al PLoS One. 2011;6(8):e23055. doi: 10.1371
    Type
    Empty backbone
    Expression
    Bacterial
    Available Since
    Nov. 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    68361-plasmid-map-sequence-id-128755

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  8. pMPO 53

    Plasmid
    #68360
    Purpose
    Expression vectors for the salycilate cascade expression system (Pnah/NahR-Psal/XylS2).ApR, expression vector with rrnBT1T2-Pm-T7 SD sequence-MCSII-sspH2 signal peptide, ColE1 replication origin
    Depositor
    Eduardo Santero
    Article
    Medina et al PLoS One. 2011;6(8):e23055. doi: 10.1371
    Type
    Empty backbone
    Expression
    Bacterial
    Available Since
    Oct. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    68360-plasmid-map-sequence-id-127705

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  14. Ring1-KO

    Plasmid
    #246224
    Purpose
    Knock out of Ring1
    Depositor
    Hengbin Wang
    Insert
    Ring1 gRNA (RING1 Human)
    Use
    Lentiviral
    Available Since
    Oct. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    246224-plasmid-map-sequence-id-487567

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  19. pTG131

    Plasmid
    #198640
    Purpose
    Thermosensitive pSC101-based plasmid expressing a sgRNA under the control of the J23119 promoter and Cas9 under the control of the DAPG-inducible PhlF promoter
    Depositor
    David Bikard
    Article
    Rostain et al Nucleic Acids Res. 2023 Mar 17:gkad170.
    Insert
    Cas9
    Expression
    Bacterial
    Promoter
    pPhlF
    Available Since
    June 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    198640-plasmid-map-sequence-id-396878

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Showing: 2001 - 2020 of 2603 results