We narrowed to 5,840 results for: pCas

1. pCAG-MBP-ATG9 (1-830)

   Plasmid

   #188086

   Purpose

   Expresses human ATG9 C-terminal truncation mutant in mammalian cells

   Depositor

   James Hurley

   Article

   Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

   Insert

   ATG9 (ATG9A Human)
   

   Tags

   MBP tag

   Expression

   Mammalian

   Mutation

   ATG9 (1-830)

   Promoter

   CMV

   Available Since

   Aug. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-MBP-ATG9-P833A

   Plasmid

   #188083

   Purpose

   Expresses human ATG9-P833A mutant in mammalian cells

   Depositor

   James Hurley

   Article

   Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

   Insert

   ATG9 (ATG9A Human)
   

   Tags

   MBP tag

   Expression

   Mammalian

   Mutation

   P833A

   Promoter

   CMV

   Available Since

   Aug. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-GST-ATG101-Y45D

   Plasmid

   #188105

   Purpose

   Expresses human ATG101 HORMA Y45D mutant in mammalian cells

   Depositor

   James Hurley

   Article

   Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

   Insert

   ATG101 (ATG101 Human)
   

   Tags

   GST tag

   Expression

   Mammalian

   Mutation

   codon-optimized ATG101-Y45D

   Promoter

   CMV

   Available Since

   Aug. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAGGS-eDHFR(69K6)-iRFP713

   Plasmid

   #178855

   Purpose

   Mammalian expression of eDHFR(69K6)-iRFP713

   Depositor

   Shinya Tsukiji

   Article

   Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

   Insert

   eDHFR(69K6)-iRFP713
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   July 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCasper-pNos-MCP-mCherry

   Plasmid

   #180275

   Purpose

   to epress MCP-mCherry fusion protein in the early fly embryos (driven by maternal nanos driver)

   Depositor

   Hernan Garcia

   Article

   Liu et al PLoS Comput Biol. 2021 May 18;17(5):e100

   Insert

   MCP-mCherry
   

   Tags

   mCherry

   Expression

   Insect

   Promoter

   nanos

   Available Since

   April 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJCC_164 SpCas9 R1333A/R1335A

   Plasmid

   #179528

   Purpose

   For bacterial expression of SpCas9 R1333A/R1335A (PAM-binding arginines mutant) with an N-terminal His-MBP tag

   Depositor

   Jennifer Doudna

   Article

   Cofsky et al Nat Struct Mol Biol. 2022 Apr;29(4):395-

   Insert

   SpCas9 R1333A/R1335A
   

   Tags

   10X His, TEV protease cleavage site, and maltose-…

   Expression

   Bacterial

   Mutation

   changed arginines 1333 and 1335 to alanine

   Available Since

   April 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG1.1-Armc12 delta N/FLAG

   Plasmid

   #180495

   Purpose

   Expression vector of N-terminal deleted mouse Armc12 tagged with FLAG at C-terminus. See Fig.4D of Shimada, K. et al. Proc. Natl. Acad. Sci. USA. 118 (6): e2018355118, 2021.

   Depositor

   Masahito Ikawa

   Article

   Shimada et al Proc Natl Acad Sci U S A. 2021 Feb 9;118

   Insert

   armadillo repeat containing 12 (Armc12 Mouse)
   

   Tags

   FLAG tag

   Expression

   Mammalian

   Mutation

   N-terminus of Armc12 is deleted

   Promoter

   CAG promoter

   Available Since

   March 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG1.1-Armc12 delta C/FLAG

   Plasmid

   #180497

   Purpose

   Expression vector of C-terminal deleted mouse Armc12 tagged with FLAG at C-terminus. See Fig.4D of Shimada, K. et al. Proc. Natl. Acad. Sci. USA. 118 (6): e2018355118, 2021.

   Depositor

   Masahito Ikawa

   Article

   Shimada et al Proc Natl Acad Sci U S A. 2021 Feb 9;118

   Insert

   armadillo repeat containing 12 (Armc12 Mouse)
   

   Tags

   FLAG tag

   Expression

   Mammalian

   Mutation

   C-terminus of Armc12 is deleted

   Promoter

   CAG promoter

   Available Since

   March 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-hSPATA33-8xHIS-1D4

   Plasmid

   #179133

   Purpose

   Expression vector of human spermatogenesis associated 33 (SPATA33) tagged with 8XHIS and 1D4 at C-terminus, CAG promoter, rabbit globin poly(A) signal.

   Depositor

   Masahito Ikawa

   Article

   Miyata et al Proc Natl Acad Sci U S A. 2021 Aug 31;11

   Insert

   spermatogenesis associated 33 (SPATA33 Human)
   

   Tags

   1D4 and 8XHIS

   Expression

   Mammalian

   Available Since

   Jan. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-WIPI2dR108E-cs-TEV-STREP

    Plasmid

    #171835

    Purpose

    Mammalian expression of human WIPI2d R108E with C-terminal Strep

    Depositor

    James Hurley

    Article

    Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

    Insert

    WIPI2d (WIPI2 Human)
    

    Tags

    Strep

    Expression

    Mammalian

    Mutation

    R108E

    Available Since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-WIPI2dR125E-cs-TEV-STREP

    Plasmid

    #171836

    Purpose

    Mammalian expression of human WIPI2d R125E with C-terminal Strep

    Depositor

    James Hurley

    Article

    Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

    Insert

    WIPI2d (WIPI2 Human)
    

    Tags

    Strep

    Expression

    Mammalian

    Mutation

    R125E

    Available Since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-WIPI2dK128E-cs-TEV-STREP

    Plasmid

    #171837

    Purpose

    Mammalian expression of human WIPI2d K128E with C-terminal Strep

    Depositor

    James Hurley

    Article

    Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

    Insert

    WIPI2d (WIPI2 Human)
    

    Tags

    Strep

    Expression

    Mammalian

    Mutation

    K128E

    Available Since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-WIPI2dH85E-cs-TEV-STREP

    Plasmid

    #171831

    Purpose

    Mammalian expression of human WIPI2d H85E with C-terminal Strep

    Depositor

    James Hurley

    Article

    Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

    Insert

    WIPI2d (WIPI2 Human)
    

    Tags

    Strep

    Expression

    Mammalian

    Mutation

    H85E

    Available Since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-WIPI2dK88E-cs-TEV-STREP

    Plasmid

    #171832

    Purpose

    Mammalian expression of human WIPI2d K88E with C-terminal Strep

    Depositor

    James Hurley

    Article

    Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

    Insert

    WIPI2d (WIPI2 Human)
    

    Tags

    Strep

    Expression

    Mammalian

    Mutation

    K88E

    Available Since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-WIPI2dI92E-cs-TEV-STREP

    Plasmid

    #171833

    Purpose

    Mammalian expression of human WIPI2d I92E with C-terminal Strep

    Depositor

    James Hurley

    Article

    Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

    Insert

    WIPI2d (WIPI2 Human)
    

    Tags

    Strep

    Expression

    Mammalian

    Mutation

    I92E

    Available Since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-WIPI2dC93E-cs-TEV-STREP

    Plasmid

    #171834

    Purpose

    Mammalian expression of human WIPI2d C93E with C-terminal Strep

    Depositor

    James Hurley

    Article

    Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

    Insert

    WIPI2d (WIPI2 Human)
    

    Tags

    Strep

    Expression

    Mammalian

    Mutation

    C93E

    Available Since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-Bmpr2 gRNA resistant

    Plasmid

    #163408

    Purpose

    Expresses BMPR-2 resistant to the gRNAs(#163388-90) in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Bmpr2 (Bmpr2 Mouse)
    

    Expression

    Mammalian

    Mutation

    gRNA-targeting sequences are substituted with nuc…

    Available Since

    Aug. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-Bmpr2ΔEC gRNA resistant

    Plasmid

    #163413

    Purpose

    Expresses BMPR-2ΔEC resistant to the gRNAs(#163388-90) in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Bmpr2 (Bmpr2 Mouse)
    

    Expression

    Mammalian

    Mutation

    The extracellular domain (27-150 aa) is deleted. …

    Available Since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-Bmpr2ΔTail gRNA resistant

    Plasmid

    #163412

    Purpose

    Expresses BMPR-2ΔTail resistant to the gRNAs(#163388-90) in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Bmpr2 (Bmpr2 Mouse)
    

    Expression

    Mammalian

    Mutation

    The C-terminal tail domain (530-1038 aa) is delet…

    Available Since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pENTR-L3-eSpCas9(1.1)-2A-EGFP-L2

    Plasmid

    #141051

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Insert

    eSpCas9(1.1)-2A-EGFP
    

    Expression

    Mammalian

    Available Since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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