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pBoL1P4GA

Plasmid

#112910

Purpose

Express a cloned sgRNA from AtU626 promoter

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2019;1917:155-170. doi

Insert

AtU626 PROMOTER-Laz-sgRNA

Promoter

AtU626

Available Since

March 22, 2021

Availability

Academic Institutions and Nonprofits only

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pTU-A-010

Plasmid

#124418

Purpose

Slot A, contains mKO2-MarA (step 1)

Depositor

Yolanda Schaerli

Article

Santos-Moreno et al ACS Synth Biol. 2019 Jul 19;8(7):1691-16

Insert

slot A with mKO2-MarA reporter + terminator + spacer

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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pTU-B-010

Plasmid

#124419

Purpose

Slot B, contains sfGFP-MarAn20 (step 1)

Depositor

Yolanda Schaerli

Article

Santos-Moreno et al ACS Synth Biol. 2019 Jul 19;8(7):1691-16

Insert

slot B with sfGFP-MarAn20 reporter + terminator + spacer

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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pTU-C-010

Plasmid

#124420

Purpose

Slot C, contains RepA70-mKate2 (step 1)

Depositor

Yolanda Schaerli

Article

Santos-Moreno et al ACS Synth Biol. 2019 Jul 19;8(7):1691-16

Insert

slot C with RepA70-mKate2 reporter + terminator

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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pRS314 snu114-14

Plasmid

#111311

Purpose

snu114-14

Depositor

Christine Guthrie

Article

Brenner et al Genetics. 2005 Jul;170(3):1063-80. doi:

Insert

SNU114

Expression

Yeast

Mutation

snu114-14

Available Since

July 30, 2018

Availability

Academic Institutions and Nonprofits only

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pRS314 HA-SNU114

Plasmid

#111318

Purpose

HA-SNU114

Depositor

Christine Guthrie

Article

Brenner et al Genetics. 2005 Jul;170(3):1063-80. doi:

Insert

SNU114

Tags

HA

Expression

Yeast

Available Since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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pFUSC8

Plasmid

#50428

Purpose

Plasmid compatible with the Golden Gate TALEN cloning system. To clone TAL effectors 8.5 RVDs long.

Depositor

Andrew Scharenberg

Article

Boissel et al Nucleic Acids Res. 2013 Nov 26.

Type

Empty backbone

Available Since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pFUSC7

Plasmid

#50427

Purpose

Plasmid compatible with the Golden Gate TALEN cloning system. To clone TAL effectors 7.5 RVDs long.

Depositor

Andrew Scharenberg

Article

Boissel et al Nucleic Acids Res. 2013 Nov 26.

Type

Empty backbone

Available Since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pFUSC6

Plasmid

#50426

Purpose

Plasmid compatible with the Golden Gate TALEN cloning system. To clone TAL effectors 6.5 RVDs long.

Depositor

Andrew Scharenberg

Article

Boissel et al Nucleic Acids Res. 2013 Nov 26.

Type

Empty backbone

Available Since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pFUSC5

Plasmid

#50425

Purpose

Plasmid compatible with the Golden Gate TALEN cloning system. To clone TAL effectors 5.5 RVDs long.

Depositor

Andrew Scharenberg

Article

Boissel et al Nucleic Acids Res. 2013 Nov 26.

Type

Empty backbone

Available Since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pFUSC4

Plasmid

#50424

Purpose

Plasmid compatible with the Golden Gate TALEN cloning system. To clone TAL effectors 4.5 RVDs long.

Depositor

Andrew Scharenberg

Article

Boissel et al Nucleic Acids Res. 2013 Nov 26.

Type

Empty backbone

Available Since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pFUSC3

Plasmid

#50423

Purpose

Plasmid compatible with the Golden Gate TALEN cloning system. To clone TAL effectors 3.5 RVDs long.

Depositor

Andrew Scharenberg

Article

Boissel et al Nucleic Acids Res. 2013 Nov 26.

Type

Empty backbone

Available Since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pFUSC2

Plasmid

#50422

Purpose

Plasmid compatible with the Golden Gate TALEN cloning system. To clone TAL effectors 2.5 RVDs long.

Depositor

Andrew Scharenberg

Article

Boissel et al Nucleic Acids Res. 2013 Nov 26.

Type

Empty backbone

Available Since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pFUSC10

Plasmid

#50430

Purpose

Plasmid compatible with the Golden Gate TALEN cloning system. To clone TAL effectors 10.5 RVDs long.

Depositor

Andrew Scharenberg

Article

Boissel et al Nucleic Acids Res. 2013 Nov 26.

Type

Empty backbone

Available Since

Jan. 14, 2014

Availability

Academic Institutions and Nonprofits only

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pCVL SFFV d14GFP Donor

Plasmid

#31475

Depositor

Andrew Scharenberg

Article

Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

Insert

SFFV d14GFP

Use

Lentiviral

Mutation

Deleted 14 amino acids on the c terminus of eGFP

Available Since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only

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bactin:GFP-LAP2b

Plasmid

#105980

Purpose

transgenesis, ubiquitous expression

Depositor

Caren Norden

Article

Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

Insert

LAP2b

Tags

GFP

Expression

Bacterial

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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iRFP-PH-PLCdelta1

Plasmid

#66841

Purpose

expression of near-infrared PI(4,5)P2 biosensor

Depositor

Pietro De Camilli

Article

Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

Insert

PH-PLCdelta1

Tags

iRFP

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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pDD112

Plasmid

#119893

Purpose

pNG2 backbone with an optimized chloramphenicol resitance marker for Rhodococcus opacus PD630

Depositor

Tae Seok Moon

Article

DeLorenzo et al ACS Synth Biol. 2018 Feb 16;7(2):727-738

Type

Empty backbone

Expression

Bacterial

Available Since

Feb. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pDD131

Plasmid

#119886

Purpose

Optimized aTc inducible pTet promoter for Rhodococcus opacus expressing mCherry on pAL5000/pMB1 backbone with kanamycin resistance.

Depositor

Tae Seok Moon

Article

DeLorenzo et al ACS Synth Biol. 2017 Oct 20;6(10):1973-1

Insert

pTet_mCherry

Expression

Bacterial

Available Since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

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LV 1-5

Plasmid

#68411

Purpose

Lentiviral backbone for use with GMAP

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Type

Empty backbone

Use

Lentiviral; Gmap

Expression

Mammalian

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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