We narrowed to 9,738 results for: tac

1. pCDNA5/avitag-apex2-v5-fkbp8

   Plasmid

   #154460

   Purpose

   expresses avitag-apex2-v5-fkbp8/biochemical assay

   Depositor

   Hyun-Woo Rhee

   Article

   Kwak et al Proc Natl Acad Sci U S A. 2020 May 15. p

   Insert

   avitag-apex2-v5-fkbp8 (FKBP8 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-F, T7 promoter

   Available since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDNA3/flag-pbira(1-g78)-sec61b

   Plasmid

   #154455

   Purpose

   expresses flag-pbira(1-g78)-sec61b/biochemical assay

   Depositor

   Hyun-Woo Rhee

   Article

   Kwak et al Proc Natl Acad Sci U S A. 2020 May 15. p

   Insert

   flag-pbira(1-g78)-sec61b (SEC61B Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   T7 promoter

   Available since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLCHKO_TK1_HPRT1_Lb_4

   Plasmid

   #155062

   Purpose

   Lentiviral plasmid for expressing U6 driven multiplexed hybrid guide (hg)RNA targeting TK1 using SpCas9 and three intergenic sites & HPRT1 using LbCas12a (CHyMErA system)

   Depositor

   Benjamin Blencowe, Jason Moffat

   Article

   Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

   Insert

   (hg)RNA targeting TK1 using SpCas9 and three intergenic sites & HPRT1 using LbCas12a
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. sg_Trp53_i4-ipUSEPR-TR657

   Plasmid

   #228930

   Purpose

   Knockdown of Trp53 in mammalian cells

   Depositor

   Sika Zheng

   Article

   Lin et al Neuron. 2024 Jul 3;112(13):2157-2176.e12

   Insert

   sg_Trp53_i4 (Trp53 Mouse, sequence: GTCGCTACCTACAGCCAGGA)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFlare9A-Clip2E9 Mutant

   Plasmid

   #209575

   Purpose

   minigene construct for Clip2 E9 alternative splicing, potential PTBP2 binding sites are deleted

   Depositor

   Sika Zheng

   Article

   Zhang et al Neuron. 2019 Feb 20;101(4):690-706.e10.

   Insert

   Clip2 (Clip2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   ACGCGTTTCTGAATTCTTCTAACTGCCCTCAAATGCACGGTGGCATGTG…

   Promoter

   CMV

   Available since

   March 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLKO.1 puro shCALM2-1

   Plasmid

   #193699

   Purpose

   Constitutive lentiviral expression of CALM2 shRNA

   Depositor

   William Hahn

   Article

   Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

   Insert

   CALM2 (CALM2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCoHygro L16_shRNA of spliced Copia

   Plasmid

   #71389

   Purpose

   knock down expression of spliced Copia in Drosophila melanogaster

   Depositor

   Timothy Nilsen

   Insert

   Copia
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   U6

   Available since

   Nov. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-hSyn-mCherry-Rorb sesRNA-2a-msFlag-2a-tTA2-WPRE

   Plasmid

   #239029

   Purpose

   Expression of mCherry, sesRNA in mammalian cells, with msFlag and tTA2 as efRNA.

   Depositor

   Josh Huang

   Article

   Qian et al Nature. 2022 Oct;610(7933):713-721. doi:

   Insert

   sesRNA for mouse Rorb (Rorb Mouse)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-hSyn-mCherry-Satb2 sesRNA-2a-msFlag-2a-tTA-WPRE

   Plasmid

   #239028

   Purpose

   Expression of mCherry, sesRNA in mammalian cells, with msFlag and tTA2 as efRNA.

   Depositor

   Josh Huang

   Article

   Qian et al Nature. 2022 Oct;610(7933):713-721. doi:

   Insert

   sesRNA for mouse Satb2 (Satb2 Mouse)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Adeno FT

    Plasmid

    #101824

    Purpose

    Adenovirus for the expression of gRNAs targeting intron 17 of murine Fgfr3 and intron 6 of murine Tacc3

    Depositor

    Andrea Ventura

    Article

    Cook et al Nat Commun. 2017 Jul 11;8:15987. doi: 10

    Insert

    U6_sgRNA(Fgfr3)_U6_sgRNA(Tacc3)_CBh_FLAG-Cas9 (Fgfr3, Tacc3 Mouse)
    

    Use

    Adenoviral

    Tags

    FLAG-Cas9

    Expression

    Mutation

    Promoter

    U6 and CBh

    Available since

    Nov. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

11. pRARE-MBP-DEST

    Plasmid

    #84650

    Purpose

    Gateway cloning vector expresses MBP-tagged proteins under the control of the IPTG– inducible ptac promoter and also expresses several tRNAs that are rare in E. coli.

    Depositor

    Neil Olszewski

    Article

    Steiner et al Plant Cell. 2012 Jan;24(1):96-108. doi:

    Type

    Empty backbone

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Sept. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. PDLIM4_PDZ_1

    Plasmid

    #103941

    Purpose

    Protein expression and purification of RIL BGLII PDZ domain

    Depositor

    Sachdev Sidhu

    Article

    Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

    Insert

    PDLIM4 (PDLIM4 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEX-6P-1 SARS-CoV 3CLpro

    Plasmid

    #61692

    Purpose

    inducible expression of SARS 3CLpro protease

    Depositor

    Michael Johnson

    Article

    Lee et al Bioorg Med Chem. 2014 Jan 1;22(1):167-77

    Insert

    SARS 3CLpro protease
    

    Use

    Tags

    GST and HIS

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    April 10, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pGEX Stat5A-SH2

    Plasmid

    #46515

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Stat5A (STAT5A Human)
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 582-693

    Promoter

    Tac

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEX Stat5B-SH2

    Plasmid

    #46516

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Stat5B (STAT5B Human)
    

    Use

    Tags

    AviTag, GST, and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 593-692

    Promoter

    Tac

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEX ShcD-SH2

    Plasmid

    #46488

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    ShcD (SHC4 Human)
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 512-623

    Promoter

    Tac

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGEX-4T-1-RIPK3

    Plasmid

    #78827

    Purpose

    To purify GST-tagged recombinant RIPK3 protein

    Depositor

    Jaewhan Song

    Article

    Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

    Insert

    RIPK3 (RIPK3 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGEX Tec-SH2

    Plasmid

    #46522

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Tec (TEC Human)
    

    Use

    Tags

    AviTag, GST, and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 236-351

    Promoter

    Tac

    Available since

    Oct. 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. TJP1_PDZ_2

    Plasmid

    #103910

    Purpose

    Protein expression and purification of ZO1 PDZ2 domain

    Depositor

    Sachdev Sidhu

    Article

    Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

    Insert

    TJP1 (TJP1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. TJP1_PDZ_1

    Plasmid

    #103909

    Purpose

    Protein expression and purification of ZO1 PDZ1 domain

    Depositor

    Sachdev Sidhu

    Article

    Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

    Insert

    TJP1 (TJP1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge