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P0050::kaiBC S431A T432A NS1

Plasmid

#101837

Purpose

drives expression of kaiBC S431A T432A variant from an rpaA-independent promoter in NS1 locus

Depositor

Erin O'Shea

Article

Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

Insert

kaiBC S431A T432A with p0050 promoter

Use

Cyanobacteria cloning vector

Mutation

S431A T432A

Available Since

Oct. 20, 2017

Availability

Academic Institutions and Nonprofits only

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P0050::kaiBC S431E T432E NS1

Plasmid

#101836

Purpose

drives expression of kaiBC S431E T432E variant from an rpaA-independent promoter in NS1 locus

Depositor

Erin O'Shea

Article

Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

Insert

kaiBC S431E T432E with p0050 promoter

Use

Cyanobacteria cloning vector

Mutation

S431E T432E

Available Since

Oct. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pACYC_HA-ZmSAE1_ZmSAE2(TRUNC)-FLAG

Plasmid

#91945

Purpose

Bacterial expression of maize SAE1 and truncated SAE2

Depositor

Richard Vierstra

Article

Augustine et al Plant Physiol. 2016 Jul;171(3):2191-210.

Inserts

HA-ZmSAE1
ZmSAE2-FLAG

Tags

FLAG and HA

Expression

Bacterial

Mutation

Truncation of ZmSAE2 to mimic a splice variant

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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p3R112C/T180C-GG

Plasmid

#85093

Purpose

Host vector to fuse a gene at the C-terminus of PCNA3 variant (R112C/T180C)

Depositor

Teruyuki Nagamune

Article

Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

Insert

DNA polymerase sliding clamp 3

Tags

His6 tag

Expression

Bacterial

Mutation

changed Arginine 112 to Cysteine and Threonine 18…

Promoter

T7 promoter

Available Since

Nov. 29, 2016

Availability

Academic Institutions and Nonprofits only

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p1G108C-GG

Plasmid

#85092

Purpose

Host vector to fuse a gene at the C-terminus of PCNA1 variant (G108C)

Depositor

Teruyuki Nagamune

Article

Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

Insert

DNA polymerase sliding clamp 1

Tags

His6 tag

Expression

Bacterial

Mutation

changed Glycine 108 to Cysteine

Promoter

T7 promoter

Available Since

Nov. 29, 2016

Availability

Academic Institutions and Nonprofits only

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MSCV-6NBC-GFP-PGK-Puro-Barcode9

Plasmid

#83686

Purpose

individual 6N barcode to make barcoded cell line variants

Depositor

Monte Winslow

Article

Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

Insert

Barcode9

Use

Retroviral

Mutation

please view depositor comments below

Available Since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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MSCV-6NBC-GFP-PGK-Puro-Barcode7

Plasmid

#83684

Purpose

individual 6N barcode to make barcoded cell line variants

Depositor

Monte Winslow

Article

Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

Insert

Barcode7

Use

Retroviral

Mutation

L119P in GFP

Available Since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pGEX-KG/Net1A

Plasmid

#28261

Depositor

Jeffrey Frost

Article

Carr et al J Biol Chem. 2009 Sep 4. 284(36):24269-8

Insert

Net1A (Net1 Mouse)

Tags

GST

Expression

Bacterial

Available Since

April 11, 2011

Availability

Academic Institutions and Nonprofits only
pSB700 Cerulean-Weissman scaffold

Plasmid

#79377

Purpose

Cas9 gRNA variant to recruit ms2 proteins

Depositor

George Church

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

scaffold

Use

CRISPR

Expression

Mammalian

Availability

Academic Institutions and Nonprofits only

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pSB700 Cerulean-Zhang2.0

Plasmid

#79378

Purpose

Cas9 gRNA variant to recruit ms2 proteins

Depositor

George Church

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

zhang 2.0

Use

CRISPR

Expression

Mammalian

Availability

Academic Institutions and Nonprofits only

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