We narrowed to 5,708 results for: ER;

1. pTorPE-Y-GECO2s

   Plasmid

   #105067

   Purpose

   A yellow fluorescent Ca2+ indicator variant with high binding affinity and slow kinetics for expression in bacterial cells

   Depositor

   Robert Campbell

   Article

   Zhao et al Sci Rep. 2018 Aug 6;8(1):11758. doi: 10.

   Insert

   Y-GECO2s
   

   Use

   Tags

   6x His tag

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pTorPE-Y-GECO2.1s

   Plasmid

   #105068

   Purpose

   A yellow fluorescent Ca2+ indicator variant with high binding affinity and slow kinetics for expression in bacterial cells

   Depositor

   Robert Campbell

   Article

   Zhao et al Sci Rep. 2018 Aug 6;8(1):11758. doi: 10.

   Insert

   Y-GECO2.1s
   

   Use

   Tags

   6x His tag

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pF(UG) hSyn SYP-deltaTMD SYT7alpha

   Plasmid

   #179718

   Purpose

   Lentiviral expression of synaptic vesicle-targeted mouse Syt7

   Depositor

   Edwin Chapman

   Article

   Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

   Insert

   SYT7 (Syt7 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pF(UG) hSyn Lamp1-SYT7alpha deltaTMD

   Plasmid

   #179722

   Purpose

   Lentiviral expression of lysosome-targeted mouse Syt7

   Depositor

   Edwin Chapman

   Article

   Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

   Insert

   SYT7 (Syt7 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pF(UG) U6-SYT7 sgRNA 777 HaloTag

   Plasmid

   #179724

   Purpose

   U6-driven SYT7 gRNA and HaloTag lentiviral vector for CRISPR/HITI

   Depositor

   Edwin Chapman

   Article

   Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

   Insert

   sgRNA and HaloTag
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pF(UG) CamKII sf iGluSnFR S72A

   Plasmid

   #179726

   Purpose

   Lentiviral expression of iGluSnFR biosensor for monitoring synaptic vesicle exocytosis and glutamate release

   Depositor

   Edwin Chapman

   Article

   Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

   Insert

   iGluSnFR
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   S72A

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pFSW Syt1-C2B R398,399Q IRES GFP

   Plasmid

   #133819

   Purpose

   Encodes the C2B domain of synaptotagmin 1 with mutations R398,399Q for viral expression

   Depositor

   Edwin Chapman

   Article

   Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

   Insert

   Syt1 (Syt1 Rat)
   

   Use

   Lentiviral

   Tags

   IRES-GFP

   Expression

   Mutation

   R398,399Q

   Promoter

   hSyn (human synapsin I promoter)

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pFSW Syt1-C2B K326,327E IRES GFP

   Plasmid

   #133820

   Purpose

   Encodes the C2B domain of synaptotagmin 1 with mutations K326,327E for viral expression

   Depositor

   Edwin Chapman

   Article

   Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

   Insert

   Syt1 (Syt1 Rat)
   

   Use

   Lentiviral

   Tags

   IRES-GFP

   Expression

   Mutation

   K326,327E

   Promoter

   hSyn (human synapsin I promoter)

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pFSW Syt1-C2B D363,365N IRES GFP

   Plasmid

   #133822

   Purpose

   Encodes the C2B domain of synaptotagmin 1 with calcium-coordinating ligand mutations D363,365N for viral expression

   Depositor

   Edwin Chapman

   Article

   Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

   Insert

   Syt1 (Syt1 Rat)
   

   Use

   Lentiviral

   Tags

   IRES-GFP

   Expression

   Mutation

   D363,365N

   Promoter

   hSyn (human synapsin I promoter)

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFSW Syt1-C2A D230,232N IRES GFP

    Plasmid

    #133824

    Purpose

    Encodes the C2A domain of synaptotagmin 1 with calcium-coordinating ligand mutations D230,232N for viral expression

    Depositor

    Edwin Chapman

    Article

    Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

    Insert

    Syt1 (Syt1 Rat)
    

    Use

    Lentiviral

    Tags

    IRES-GFP

    Expression

    Mutation

    D230,232N

    Promoter

    hSyn (human synapsin I promoter)

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGEX Syt1 C2AB (96-421) D303G F234C

    Plasmid

    #170647

    Purpose

    Expression of syt1 for purification in E. Coli

    Depositor

    Edwin Chapman

    Article

    Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

    Insert

    SYT1 (Syt1 Rat)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D303G, C277A, F234C

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGEX Syt1 C2AB (96-421) D303G V304C

    Plasmid

    #170648

    Purpose

    Expression of syt1 for purification in E. Coli

    Depositor

    Edwin Chapman

    Article

    Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

    Insert

    SYT1 (Syt1 Rat)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D303G, C277A, V304C

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGEX Syt1 C2AB (96-421) D365E F234C

    Plasmid

    #170650

    Purpose

    Expression of syt1 for purification in E. Coli

    Depositor

    Edwin Chapman

    Article

    Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

    Insert

    SYT1 (Syt1 Rat)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D365E, C277A, F234C

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGEX Syt1 C2AB (96-421) D365E V304C

    Plasmid

    #170651

    Purpose

    Expression of syt1 for purification in E. Coli

    Depositor

    Edwin Chapman

    Article

    Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

    Insert

    SYT1 (Syt1 Rat)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D365E, C277A, V304C

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGEX Syt1 C2AB (96-421) I367T F234C

    Plasmid

    #170653

    Purpose

    Expression of syt1 for purification in E. Coli

    Depositor

    Edwin Chapman

    Article

    Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

    Insert

    SYT1 (Syt1 Rat)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    I367T, C277A, F234C

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGEX Syt1 C2AB (96-421) I367T V304C

    Plasmid

    #170654

    Purpose

    Expression of syt1 for purification in E. Coli

    Depositor

    Edwin Chapman

    Article

    Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

    Insert

    SYT1 (Syt1 Rat)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    I367T, C277A, V304C

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE (AAV1)

    Viral Prep

    #198516-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE (#198516). In addition to the viral particles, you will also receive purified pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE plasmid DNA. Ef1a-driven, Cre-dependent expression of the optimized opsin PdCO in frame with mScarlet. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Ofer Yizhar

    Article

    Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

    Promoter

    EF-1a

    Tags

    mScarlet

    Available since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

18. pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE (AAV5)

    Viral Prep

    #198516-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE (#198516). In addition to the viral particles, you will also receive purified pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE plasmid DNA. Ef1a-driven, Cre-dependent expression of the optimized opsin PdCO in frame with mScarlet. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Ofer Yizhar

    Article

    Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

    Promoter

    EF-1a

    Tags

    mScarlet

    Available since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

19. GFP-Mff

    Plasmid

    #49153

    Purpose

    mammalian expression of Mff fused to GFP

    Depositor

    Gia Voeltz

    Article

    Friedman et al Science. 2011 Oct 21;334(6054):358-62. d

    Insert

    Mff (MFF Human)
    

    Use

    Tags

    acGFP1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. cfSGFP2-col4a1

    Plasmid

    #160317

    Purpose

    cfSGFP2-tagged collagen4a1 for mammalian expression

    Depositor

    Ikuo Wada

    Article

    Matsui et al Cell Struct Funct. 2020 Jul 23;45(2):107

    Insert

    COL4A1 (COL4A1 Human)
    

    Use

    Tags

    cfSGFP2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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