We narrowed to 6,781 results for: UBC

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JD633

Plasmid

#160393

Purpose

CRISPR-Cas9 vector including GRF4-GIF1. For wheat and other monocots CRISPR experiments. Includes two AarI sites for gRNA cloning

Depositor

Jorge Dubcovsky

Article

Debernardi et al Nat Biotechnol. 2020 Oct 12. pii: 10.103

Inserts

ZmUbi::wheat GRF4-GIF1 chimera
ZmUbi:TaCas9 + TaU6:gRNA

Use

Binary vector

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pSC-PreE2

Plasmid

#52264

Purpose

Bacterial expression of GST-tagged TARGET proteins fused to SUMO-E2 with PreScission protease cleavage site linker

Depositor

Primo Schaer

Article

Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

Insert

Ubc9 GST fusion (UBE2I Human)

Tags

GST

Expression

Bacterial

Available Since

July 10, 2014

Availability

Academic Institutions and Nonprofits only

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pSC-IntE2

Plasmid

#52265

Purpose

Bacterial expression of GST-tagged TARGET proteins fused to SUMO-E2 with GyrA intein linker

Depositor

Primo Schaer

Article

Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

Insert

Ubc9 GST fusion (UBE2I Human, Synthetic)

Tags

GST and GyrA-intein

Expression

Bacterial

Available Since

July 10, 2014

Availability

Academic Institutions and Nonprofits only

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TAL3467

Plasmid

#47186

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-inhibitorofDNAbinding3-right (id3 Zebrafish)

Use

T7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 13, 2013

Availability

Academic Institutions and Nonprofits only
TAL3466

Plasmid

#47185

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-inhibitorofDNAbinding3-left (id3 Zebrafish)

Use

T7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 13, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA5FRT/TO-Strep/HA-UBE2J1

Plasmid

#124669

Purpose

Expresses UBE2J1 with N-terminal Strep-HA tag in mammalian cells

Depositor

Hemmo Meyer

Article

Koerver et al EMBO Rep. 2019 Aug 21:e48014. doi: 10.15

Insert

UBE2J1 (UBE2J1 Human)

Tags

2xStrep/HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pScarlessHD-C-3xVHH05-DsRed

Plasmid

#171580

Purpose

For scarless insertion of 3xVHH05 NanoTag into C-term; Marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.

Depositor

Norbert Perrimon, Hidde Ploegh

Article

Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

Insert

3xVHH05 (UBE2J1 Human)

Expression

Insect

Available Since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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pScarlessHD-N-3xVHH05-DsRed

Plasmid

#171581

Purpose

For scarless insertion of 3xVHH05 NanoTag into N-term; Marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.

Depositor

Norbert Perrimon, Hidde Ploegh

Article

Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

Insert

3xVHH05 (UBE2J1 Human)

Expression

Insect

Available Since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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pET3a-UBE2J1 1-283

Plasmid

#185348

Purpose

In vitro transcription and translation in bacterial system

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

UBE2J1 (UBE2J1 Human)

Expression

Bacterial

Mutation

Cytoplasmic domain only, aa 1-283

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pInducer10-SAE1_sh1

Plasmid

#168984

Purpose

Expresses RFP cDNA and SAE1 shRNA 1 in mammalian cells

Depositor

Ji Luo

Article

Yu et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

SUMO1 Activating Enzyme Subunit 1 (SAE1 Human)

Expression

Mammalian

Promoter

Ubc promoter

Available Since

June 1, 2021

Availability

Academic Institutions and Nonprofits only

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pInducer10-SAE1_sh3

Plasmid

#168985

Purpose

Expresses RFP cDNA and SAE1 shRNA 3 in mammalian cells

Depositor

Ji Luo

Article

Yu et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

SUMO1 Activating Enzyme Subunit 1 (SAE1 Human)

Expression

Mammalian

Promoter

Ubc promoter

Available Since

June 1, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Par6-ExRai-CKAR2

Plasmid

#236101

Purpose

Subcellularly targeted enhanced excitation-ratiometric biosensor for monitoring Protein Kinase C activity in living cells for selective detection of aPKC activity within the Par complex.

Depositor

Jin Zhang

Article

Su et al Nat Chem Biol. 2024 Oct 11. doi: 10.1038

Insert

Par6C-ExRai-CKAR2 (PARD6A Human, Synthetic)

Expression

Mammalian

Promoter

CMV

Available Since

May 23, 2025

Availability

Academic Institutions and Nonprofits only

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pInducer10-SAE1_sh1

Plasmid

#168984

Purpose

Expresses RFP cDNA and SAE1 shRNA 1 in mammalian cells

Depositor

Ji Luo

Article

Yu et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

SUMO1 Activating Enzyme Subunit 1 (SAE1 Human)

Expression

Mammalian

Promoter

Ubc promoter

Available Since

June 1, 2021

Availability

Academic Institutions and Nonprofits only

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pInducer10-SAE1_sh3

Plasmid

#168985

Purpose

Expresses RFP cDNA and SAE1 shRNA 3 in mammalian cells

Depositor

Ji Luo

Article

Yu et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

SUMO1 Activating Enzyme Subunit 1 (SAE1 Human)

Expression

Mammalian

Promoter

Ubc promoter

Available Since

June 1, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV4 p65

Plasmid

#21966

Depositor

Warner Greene

Article

Ballard et al Proc Natl Acad Sci U S A. 1992 Mar 1. 89

Insert

NFkB p65 subunit (RELA Human)

Expression

Mammalian

Mutation

This construct was the product of 3 subcloning pr…

Available Since

Sept. 17, 2009

Availability

Academic Institutions and Nonprofits only

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pCMV4 p65

Plasmid

#21966

Depositor

Warner Greene

Article

Ballard et al Proc Natl Acad Sci U S A. 1992 Mar 1. 89

Insert

NFkB p65 subunit (RELA Human)

Expression

Mammalian

Mutation

This construct was the product of 3 subcloning pr…

Available Since

Sept. 17, 2009

Availability

Academic Institutions and Nonprofits only

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pHOT-P2Y2R

Plasmid

#159108

Purpose

entiviral vector encoding P2Y purinoceptor 2 (P2Y2R), a high affinity G-protein coupled receptor that mobilizes Ca2+ from intracellular stores upon binding extracellular ATP.48,49 By using the Gibson

Depositor

Fabio Mammano

Article

Mazzarda et al Lab Chip. 2020 Jul 23. doi: 10.1039/d0lc

Insert

P2Y2R

Use

Lentiviral

Promoter

UBC

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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FUW-hSNCA(D2R)-NE

Plasmid

#215487

Purpose

Lentiviral expression of mutated alpha-synuclein (2nd amino acid aspartate mutated to arginine) in mammalian cells

Depositor

Ophir Shalem

Article

Santhosh Kumar et al Sci Adv. 2024 Feb 9;10(6):eadj4767. doi:

Insert

alpha-synuclein (SNCA Human)

Use

Lentiviral

Tags

NE tag

Expression

Mammalian

Mutation

D (second aa) mutated to R

Promoter

Ubc

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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FUW-hSNCA(D2E)-NE

Plasmid

#215488

Purpose

Lentiviral expression of mutated alpha-synuclein (2nd amino acid aspartate mutated to glutamate) in mammalian cells

Depositor

Ophir Shalem

Article

Santhosh Kumar et al Sci Adv. 2024 Feb 9;10(6):eadj4767. doi:

Insert

alpha-synuclein (SNCA Human)

Use

Lentiviral

Tags

NE tag

Expression

Mammalian

Mutation

D (second aa) mutated to E

Promoter

Ubc

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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FUW-hSNCA(D2R)-GFP

Plasmid

#215490

Purpose

Lentiviral expression and easy visualization of mutated alpha-synuclein (2nd amino acid aspartate mutated to arginine) in mammalian cells

Depositor

Ophir Shalem

Article

Santhosh Kumar et al Sci Adv. 2024 Feb 9;10(6):eadj4767. doi:

Insert

alpha-synuclein (SNCA Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

D (second aa) mutated to R

Promoter

Ubc

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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