We narrowed to 150,436 results for: ins

1. pFBOH-MHL_EML4:250-877

   Plasmid

   #210914

   Purpose

   Insect expression of human EML4 fragment I250 to A877. N-terminal 6X His tag with TEV cleavage.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

   Insert

   EML4:I250-A877 (EML4 Human)
   

   Tags

   MHHHHHHSSGRENLYFQG

   Expression

   Insect

   Mutation

   K283E natural variant (VAR_031726), I382V Natural…

   Promoter

   Polyhedrin

   Available Since

   March 19, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pFBD-BirA_ATG16L1:303-607

   Plasmid

   #210942

   Purpose

   Insect expression of human ATG16L1 fragment G303 to Y607. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

   Insert

   ATG16L1:G303-Y607 (ATG16L1 Human)
   

   Tags

   GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG

   Expression

   Insect

   Mutation

   wild type

   Promoter

   Polyhedrin

   Available Since

   March 4, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pFBD-BirA_LRRK2:2141-2527

   Plasmid

   #210901

   Purpose

   Insect expression of human LRRK2 fragment T2141 to E2527. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

   Insert

   LRRK2:T2141-E2527 (LRRK2 Human)
   

   Tags

   GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG

   Expression

   Insect

   Mutation

   M2397T natural variant ( VAR_024965)

   Promoter

   Polyhedrin

   Available Since

   March 1, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pBacMam2-DiEx-LIC_LRRK2:2141-2527

   Plasmid

   #210900

   Purpose

   Insect expression of human LRRK2 fragment T2141 to E2527. C-terminal 10X His and Flag tag with TEV cleavage site.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

   Insert

   LRRK2:T2141-E2527 (LRRK2 Human)
   

   Tags

   AENLYFQSHHHHHHHHHHDYKDDDDK and M

   Expression

   Insect

   Mutation

   M2397T natural variant ( VAR_024965)

   Promoter

   P10 and CMV

   Available Since

   March 1, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pFBD-BirA_CDC40:236-578

   Plasmid

   #210885

   Purpose

   Insect expression of human CDC40 fragment E236 to W578. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

   Insert

   CDC40:E236-W578 (CDC40 Human)
   

   Tags

   GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG

   Expression

   Insect

   Mutation

   wild type

   Promoter

   Polyhedrin

   Available Since

   March 1, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pAIP4_4REH

   Plasmid

   #234195

   Purpose

   Heterologous protein expression of major latex-like protein in Escherichia coli

   Depositor

   Christopher Bahl

   Insert

   4REH
   

   Tags

   10x HisTag-Smt3

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 6, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. EYFP Fibronectin

   Plasmid

   #56600

   Purpose

   Localization: Extracellular Matrix, Excitation: 513, Emission: 527

   Depositor

   Michael Davidson

   Insert

   Fibronectin
   

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Fibronectin begins amino acids at amino acid 900 …

   Promoter

   CMV

   Available Since

   Jan. 16, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

8. pCCG_nit9fl

   Plasmid

   #228409

   Purpose

   Expression of NIT-9 in N. crassa at the his-3 locus

   Depositor

   Robert Haensch

   Insert

   NIT-9
   

   Use

   Protein expression in n. crassa

   Available Since

   Jan. 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pIRES-mCerulean-FRB-RhoA/N12-RhoA/13C(Q63L)-FKBP-mVenus-CAAX

   Plasmid

   #214282

   Purpose

   Express split-RhoA fragments that are fused with CIDs and FPs

   Depositor

   Cliff Stains

   Article

   He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

   Inserts

   Split-RhoA fragments fused with CIDs and FPs (RHOA Human)
   ras homolog family member A (RHOA Human)
   

   Tags

   mCerulean (on insert 1) and mVenus (on insert 2)

   Expression

   Mammalian

   Mutation

   RhoA-Q63L

   Promoter

   CMV

   Available Since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pIRES-mCerulean-FRB-Cdc42/N12-Cdc42/13C(Q61L)-FKBP-mVenus-CAAX

    Plasmid

    #214278

    Purpose

    Express split-Cdc42 fragments that are fused with CIDs and FPs

    Depositor

    Cliff Stains

    Article

    He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

    Inserts

    Split-Cdc42 fragments fused with CIDs and FPs (CDC42 Human)
    Split-Cdc42 fragments fused with CIDs and FPs (CDC42 Human)
    

    Tags

    mCerulean (on insert 1) and mVenus (on insert 2)

    Expression

    Mammalian

    Mutation

    Cdc42-Q61L

    Promoter

    CMV

    Available Since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-T7-nSpRY(D10A)-BPNLS(SV40)-3xFLAG-P2A-EGFP (HES636)

    Plasmid

    #185491

    Purpose

    CMV and T7 promoter expression plasmid for human codon usage nSpRY(D10A) with a C-terminal bi-partite NLS, 3x FLAG, and P2A-eGFP

    Depositor

    Benjamin Kleinstiver, Raul Perez-Jimenez

    Article

    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

    Insert

    human codon usage nSpRY(D10A)-BPNLS-P2A-eGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-eGFP

    Expression

    Mammalian

    Mutation

    D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…

    Promoter

    CMV and T7

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-T7-NLS(SV40)-anCas(BCA)-BPNLS(SV40)-P2A-EGFP (LTH1384)

    Plasmid

    #185488

    Purpose

    CMV and T7 promoter expression plasmid for human codon usage anCas(BCA) with an N-terminal NLS, C-terminal bi-partite NLS, and P2A-eGFP

    Depositor

    Benjamin Kleinstiver, Raul Perez-Jimenez

    Article

    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

    Insert

    human codon usage NLS-anCas(BCA)-BPNLS-P2A-eGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS-P2A-eGFP and NLS

    Expression

    Mammalian

    Promoter

    CMV and T7

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCMV-T7-NLS(SV40)-anCas(PDCA)-BPNLS(SV40)-P2A-EGFP (LTH1430)

    Plasmid

    #185485

    Purpose

    CMV and T7 promoter expression plasmid for human codon usage anCas(PDCA) with an N-terminal NLS, C-terminal bi-partite NLS, and P2A-eGFP

    Depositor

    Benjamin Kleinstiver, Raul Perez-Jimenez

    Article

    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

    Insert

    human codon usage NLS-anCas(PDCA)-BPNLS-P2A-eGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS-P2A-eGFP and NLS

    Expression

    Mammalian

    Promoter

    CMV and T7

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCMV-T7-nSpCas9(H840A)-BPNLS(SV40)-3xFLAG-P2A-EGFP (HES140)

    Plasmid

    #185490

    Purpose

    CMV and T7 promoter expression plasmid for human codon usage nSpCas9(H840A) with a C-terminal bi-partite NLS, 3x FLAG, and P2A-eGFP

    Depositor

    Benjamin Kleinstiver, Raul Perez-Jimenez

    Article

    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

    Insert

    human codon usage nSpCas9(H840A)-BPNLS-P2A-eGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-eGFP

    Expression

    Mammalian

    Mutation

    H840A

    Promoter

    CMV and T7

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pIRES-mCerulean-FRB-Cdc42/N12-Cdc42/13C(T17N)-FKBP-mVenus-CAAX

    Plasmid

    #214279

    Purpose

    Express split-Cdc42 fragments that are fused with CIDs and FPs

    Depositor

    Cliff Stains

    Article

    He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

    Inserts

    Split-Cdc42 fragments fused with CIDs and FPs (CDC42 Human)
    Split-Cdc42 fragments fused with CIDs and FPs (CDC42 Human)
    

    Tags

    mCerulean (on insert 1) and mVenus (on insert 2)

    Expression

    Mammalian

    Mutation

    Cdc42-T17N

    Promoter

    CMV

    Available Since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pIRES-mCerulean-FRB-Rac1/N12-Rac1/13C(Q61L)-FKBP-mVenus-CAAX

    Plasmid

    #214280

    Purpose

    Express split-Rac1 fragments that are fused with CIDs and FPs

    Depositor

    Cliff Stains

    Article

    He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

    Inserts

    Split-Rac1 fragments fused with CIDs and FPs (RAC1 Human)
    Split-Rac1 fragments fused with CIDs and FPs (RAC1 Human)
    

    Tags

    mCerulean (on insert 1) and mVenus (on insert 2)

    Expression

    Mammalian

    Mutation

    Rac1-Q61L

    Promoter

    CMV

    Available Since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pIRES-mCerulean-FRB-Rac1/N12-Rac1/13C(T17N)-FKBP-mVenus-CAAX

    Plasmid

    #214281

    Purpose

    Express split-Rac1 fragments that are fused with CIDs and FPs

    Depositor

    Cliff Stains

    Article

    He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

    Inserts

    Split-Rac1 fragments fused with CIDs and FPs (RAC1 Human)
    Split-Rac1 fragments fused with CIDs and FPs (RAC1 Human)
    

    Tags

    mCerulean (N terminal on insert 1) and mVenus (on…

    Expression

    Mammalian

    Mutation

    Rac1-T17N

    Promoter

    CMV

    Available Since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pIRES-mCerulean-FRB-RhoA/N12-RhoA/13C(T19N)-FKBP-mVenus-CAAX

    Plasmid

    #214283

    Purpose

    Express split-RhoA fragments that are fused with CIDs and FPs

    Depositor

    Cliff Stains

    Article

    He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

    Inserts

    Split-RhoA fragments fused with CIDs and FPs (RHOA Human)
    Split-RhoA fragments fused with CIDs and FPs (RHOA Human)
    

    Tags

    mCerulean (N terminal on insert 1) and mVenus (on…

    Expression

    Mammalian

    Mutation

    RhoA-T19N

    Promoter

    CMV

    Available Since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCI AscI MR1 R9H res

    Plasmid

    #214753

    Purpose

    expresses human MR1 R9H resistant to CRISPR editing with a specific sgRNA in mammalian cells

    Depositor

    David Lewinsohn

    Article

    Kulicke et al Commun Biol. 2024 Feb 24;7(1):228. doi:

    Insert

    MHC class I-related protein 1 (MR1 Human)
    

    Tags

    IRES eGFP

    Expression

    Mammalian

    Mutation

    silent mutations to prevent editing by CRISPR/Cas…

    Promoter

    CMV

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV-BRAF-REG-T241P-Halo

    Plasmid

    #202549

    Purpose

    Mammalian expression construct encoding the BRAF regulatory (REG) domain (AA 1-435) with a C-terminal HaloTag. The REG domain contains the RASopathy mutation, T241P, within its cysteine-rich domain.

    Depositor

    Deborah Morrison

    Article

    Spencer-Smith et al Mol Cell. 2022 Nov 17;82(22):4262-4276.e

    Insert

    BRAF regulatory (REG) domain (AA 1-435) (BRAF Human)
    

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Threonine 241 mutated to proline

    Promoter

    CMV

    Available Since

    July 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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