We narrowed to 14,995 results for: invs

1. pENTR1A 2xHA GPR153

   Plasmid

   #226808

   Purpose

   Gateway entry vector for 2xHA GPR153

   Depositor

   Ramiro Iglesias-Bartolome

   Article

   Pedro et al J Invest Dermatol. 2024 Jun;144(6):1311-

   Insert

   GPR153 (GPR153 Human)
   

   Use

   Gateway entry

   Tags

   2xHA tag followed by a GDPPVAT linker

   Expression

   Mutation

   Sequence optimized from NP_997253.2

   Promoter

   Available since

   Jan. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pENTR1A 2xHA LTB4R

   Plasmid

   #226806

   Purpose

   Gateway entry vector for 2xHA LTB4R

   Depositor

   Ramiro Iglesias-Bartolome

   Article

   Pedro et al J Invest Dermatol. 2024 Jun;144(6):1311-

   Insert

   LTB4R (LTB4R Human)
   

   Use

   Gateway entry

   Tags

   2xHA tag followed by a GDPPVAT linker

   Expression

   Mutation

   Sequence optimized from NP_858043.1

   Promoter

   Available since

   Jan. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pInducer20 2xHA GPR137

   Plasmid

   #226813

   Purpose

   Tet-inducible lentiviral vector for 2xHA GPR137 expression

   Depositor

   Ramiro Iglesias-Bartolome

   Article

   Pedro et al J Invest Dermatol. 2024 Jun;144(6):1311-

   Insert

   GPR137 (GPR137 Human)
   

   Use

   Lentiviral

   Tags

   2xHA tag followed by a GDPPVAT linker

   Expression

   Mutation

   Sequence optimized from NP_001164351

   Promoter

   Available since

   Jan. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHAGE-EF1aL-E690Kmut_ABCA3-GFP

   Plasmid

   #188549

   Purpose

   Lentiviral vector allowing for EF1aL-driven constitutive expression of E690K mutant human ABCA3:GFP fusion protein

   Depositor

   Darrell Kotton

   Article

   Sun et al J Clin Invest. 2024 Jan 16;134(2):e16427

   Insert

   ABCA3:GFP (ABCA3 Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mutation

   amino acid E690->K690 (ABCA3 gene)

   Promoter

   EF1aL

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHAGE-EF1aL-W308Rmut_ABCA3-GFP

   Plasmid

   #188550

   Purpose

   Lentiviral vector allowing for EF1aL-driven constitutive expression of W308R mutant human ABCA3:GFP fusion protein

   Depositor

   Darrell Kotton

   Article

   Sun et al J Clin Invest. 2024 Jan 16;134(2):e16427

   Insert

   ABCA3 (ABCA3 Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mutation

   amino acid W308->R308 (ABCA3 gene)

   Promoter

   EF1aL

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHR-CLIP-CD28(CD45TM)

   Plasmid

   #223629

   Purpose

   Lentiviral expression of human CD28 (CD45TM) with CLIP tag in mammalian cells

   Depositor

   Enfu Hui

   Article

   Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

   Insert

   CD28 (CD28 Human)
   

   Use

   Lentiviral; mammalian expression

   Tags

   CLIP

   Expression

   Mutation

   CD28 transmembrane domain replaced by CD45 transm…

   Promoter

   Available since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHR-Spy-CD28(CD45TM)

   Plasmid

   #223624

   Purpose

   Lentiviral expression of human CD28 (CD45TM) with SpyTag003 in mammalian cells

   Depositor

   Enfu Hui

   Article

   Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

   Insert

   CD28 (CD28 Human)
   

   Use

   Lentiviral; mammalian expression

   Tags

   Spytag003

   Expression

   Mutation

   CD28 transmembrane domain replaced by CD45 transm…

   Promoter

   Available since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHR-SNAP-CD86(F56A)

   Plasmid

   #223609

   Purpose

   Lentiviral expression of human CD86 (F56A) with SNAP tag in mammalian cells

   Depositor

   Enfu Hui

   Article

   Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

   Insert

   CD86 (CD86 Human)
   

   Use

   Lentiviral; mammalian expression

   Tags

   SNAP

   Expression

   Mutation

   F56A

   Promoter

   Available since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHR-SNAP-CD80(Y65A)

   Plasmid

   #223608

   Purpose

   Lentiviral expression of human CD80 (Y65A) with SNAP tag in mammalian cells

   Depositor

   Enfu Hui

   Article

   Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

   Insert

   CD80 (CD80 Human)
   

   Use

   Lentiviral; mammalian expression

   Tags

   SNAP

   Expression

   Mutation

   Y65A

   Promoter

   Available since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHR-CLIP-CD28(SGGG)

    Plasmid

    #223607

    Purpose

    Lentiviral expression of human CD28 (SGGG) with CLIP tag in mammalian cells

    Depositor

    Enfu Hui

    Article

    Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

    Insert

    CD28 (CD28 Human)
    

    Use

    Lentiviral; mammalian expression

    Tags

    CLIP

    Expression

    Mutation

    MYPPPY mutated to SGGG

    Promoter

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGL3-ACSL4-(-1899)

    Plasmid

    #202514

    Purpose

    Expressing luciferase driven by human ACSL4 promoter (bases -1 to -1899 with respect to exon1 start site)

    Depositor

    Ming Chen

    Article

    Wang et al J Clin Invest. 2023 Mar 16:e166647. doi:

    Insert

    ACSL4 promoter (partial) (ACSL4 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUt-mNF-GFP

    Plasmid

    #199220

    Purpose

    LPUtopia-7 matching RMCE donor plasmid with GFP Negative-Feedback circuit (mNF-GFP). Use BlastR for positive selection and HSV-TK for negative selection. 

    Depositor

    Gabor Balazsi

    Article

    Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d

    Insert

    hTetR::P2A::EGFP
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV D2ir promoter

    Available since

    June 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGL3-ACSL4-(-706/-277)

    Plasmid

    #202511

    Purpose

    Expressing luciferase driven by human ACSL4 promoter (bases -277 to -706 with respect to exon1 start site)

    Depositor

    Ming Chen

    Article

    Wang et al J Clin Invest. 2023 Mar 16:e166647. doi:

    Insert

    ACSL4 promoter (partial) (ACSL4 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGL3-ACSL4-(-254)

    Plasmid

    #202512

    Purpose

    Expressing luciferase driven by human ACSL4 promoter (bases -1 to -254 with respect to exon1 start site)

    Depositor

    Ming Chen

    Article

    Wang et al J Clin Invest. 2023 Mar 16:e166647. doi:

    Insert

    ACSL4 promoter (partial) (ACSL4 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGL3-ACSL4-(-706)

    Plasmid

    #202513

    Purpose

    Expressing luciferase driven by human ACSL4 promoter (bases -1 to -706 with respect to exon1 start site)

    Depositor

    Ming Chen

    Article

    Wang et al J Clin Invest. 2023 Mar 16:e166647. doi:

    Insert

    ACSL4 promoter (partial) (ACSL4 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pINSPECT_SigP:NLuc

    Plasmid

    #197263

    Purpose

    Cloning Backbone for INSPECT expression. Encodes for intronic IRES-driven SigP:NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette. Clone homology arms via Esp3I.

    Depositor

    Gil Westmeyer

    Article

    Truong et al Nat Cell Biol. 2022 Nov;24(11):1666-1676

    Type

    Empty backbone

    Use

    CRISPR, Luciferase, Synthetic Biology, and Unspec…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAV.CBA.YFP.miR-E_shIrak1-2

    Plasmid

    #180398

    Purpose

    Producing AAV that encodes mouse Irak1 shRNA-2 with miR-E backbone

    Depositor

    Huda Zoghbi

    Article

    Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

    Insert

    shIrak1-2 (Irak1 Mouse)
    

    Use

    AAV and RNAi

    Tags

    Expression

    Mutation

    Promoter

    CBA

    Available since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. AAV.CBA.YFP.miR-E_shIrak1-3

    Plasmid

    #180399

    Purpose

    Producing AAV that encodes mouse Irak1 shRNA-3 with miR-E backbone

    Depositor

    Huda Zoghbi

    Article

    Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

    Insert

    shIrak1-3 (Irak1 Mouse)
    

    Use

    AAV and RNAi

    Tags

    Expression

    Mutation

    Promoter

    CBA

    Available since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. AAV.CBA.YFP.miR-E_shIrak1-1

    Plasmid

    #180397

    Purpose

    Producing AAV that encodes mouse Irak1 shRNA-1 with miR-E backbone

    Depositor

    Huda Zoghbi

    Article

    Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

    Insert

    shIrak1-1 (Irak1 Mouse)
    

    Use

    AAV and RNAi

    Tags

    Expression

    Mutation

    Promoter

    CBA

    Available since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pQE30-VRN1-R249E-R289E-R296E

    Plasmid

    #159532

    Purpose

    Expresses Arabidopsis thaliana VERNALIZATION1 mutant, with R249E, R289E and R296E mutations, in E. coli

    Depositor

    Joshua Mylne

    Article

    King et al J Biol Chem. 2013 Feb 1;288(5):3198-207.

    Insert

    VERNALIZATION1 (VRN1 Mustard Weed)
    

    Use

    Tags

    Six-Histidine tag

    Expression

    Bacterial

    Mutation

    Three charge reversal mutations, namely R249E R28…

    Promoter

    T5

    Available since

    Dec. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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