Addgene

Showing: 1 - 20 of 14182 results

Results per page:

pOPINVL

Plasmid

#26040

Depositor

Ray Owens

Article

Nettleship et al Protein Expr Purif. 2008 Nov . 62(1):83-

Insert

u-LacZ

Use

Tags

u-phosphatase secretion leader

Expression

Bacterial, Insect, and Mamm…

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pOPINVH

Plasmid

#26041

Depositor

Ray Owens

Article

Nettleship et al Protein Expr Purif. 2008 Nov . 62(1):83-

Insert

u-LacZ-KHHHHHH

Use

Tags

KHHHHHH and u-phosphatase secretion leader

Expression

Bacterial, Insect, and Mamm…

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pJ2042.2_invRep

Plasmid

#140688

Purpose

3-color stripe (inverted reporters)

Depositor

Yolanda Schaerli

Article

Santos-Moreno et al Nat Commun. 2020 Jun 2;11(1):2746. doi:

Insert

3-color stripe (inverted reporters)

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDF_TypeIC_target_sfGFP_invivo

Plasmid

#196399

Purpose

expresses CRISPR Cascade Type IC target sequence for in vivo assay

Depositor

David Taylor

Article

O'Brien et al Mol Cell. 2023 Mar 2;83(5):746-758.e5. d

Insert

type IC dsDNA target

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

Enlarge
pBAD_TypeIC_Cascade_cas3_invivo

Plasmid

#196400

Purpose

expresses CRISPR Cascade-Cas3 Type IC proteins for in vivo assay

Depositor

David Taylor

Article

O'Brien et al Mol Cell. 2023 Mar 2;83(5):746-758.e5. d

Inserts

Cas5c
Cas7c
Cas8c
Cas3c

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

araBAD

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28b_TypeIC_acrIF2_invivo

Plasmid

#196402

Purpose

expresses acrIF2 protein targeting CRISPR Cascade Type IC for in vivo assay

Depositor

David Taylor

Article

O'Brien et al Mol Cell. 2023 Mar 2;83(5):746-758.e5. d

Insert

anti-CRISPR IF2 protein (orf30 P. aeruginosa)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28b_TypeIC_acrIC4_invivo

Plasmid

#196403

Purpose

expresses acrIC4 protein targeting CRISPR Cascade Type IC for in vivo assay

Depositor

David Taylor

Article

O'Brien et al Mol Cell. 2023 Mar 2;83(5):746-758.e5. d

Insert

anti-CRISPR IC4 protein

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

Enlarge
pOPINVHH_BAP_his

Plasmid

#210402

Purpose

For expression of VHH with C-terminal biotin acceptor peptide and his tag

Depositor

Ray Owens

Article

Eyssen et al Bio-protocol 14(6): e4962.

Type

Empty backbone

Use

Tags

biotin acceptor peptide-his6

Expression

Bacterial

Mutation

Promoter

lac

Availability

Academic Institutions and Nonprofits only

Enlarge
pOPINVHH_cys_his

Plasmid

#210403

Purpose

For expression of VHH with C-terminal cysteine and his tag

Depositor

Ray Owens

Article

Eyssen et al Bio-protocol 14(6): e4962.

Type

Empty backbone

Use

Tags

cys-his6

Expression

Bacterial

Mutation

Promoter

lac

Availability

Academic Institutions and Nonprofits only

Enlarge
pOPINVHH_his_flag

Plasmid

#210404

Purpose

For expression of VHH with C-terminal his and Flag tags

Depositor

Ray Owens

Article

Eyssen et al Bio-protocol 14(6): e4962.

Type

Empty backbone

Use

Tags

his6-flag

Expression

Bacterial

Mutation

Promoter

lac

Availability

Academic Institutions and Nonprofits only

Enlarge
pOPINVHH_his

Plasmid

#210405

Purpose

For expression of VHH with C-terminal his tag

Depositor

Ray Owens

Article

Eyssen et al Bio-protocol 14(6): e4962.

Type

Empty backbone

Use

Tags

his6

Expression

Bacterial

Mutation

Promoter

lac

Availability

Academic Institutions and Nonprofits only

Enlarge
pOPINVHH_sortase_his

Plasmid

#210406

Purpose

For expression of VHH with C-terminal sortase and his tags

Depositor

Ray Owens

Article

Eyssen et al Bio-protocol 14(6): e4962.

Type

Empty backbone

Use

Tags

sortase-his6

Expression

Bacterial

Mutation

Promoter

lac

Availability

Academic Institutions and Nonprofits only

Enlarge
pOPINVHH_snap_his

Plasmid

#210407

Purpose

For expression of VHH with C-terminal snap protein and his tag

Depositor

Ray Owens

Article

Eyssen et al Bio-protocol 14(6): e4962.

Type

Empty backbone

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

lac

Availability

Academic Institutions and Nonprofits only

Enlarge
pOPINVHH_his_myc

Plasmid

#210526

Purpose

For expression of VHH with C-terminal his and myc tags

Depositor

Ray Owens

Article

Eyssen et al Bio-protocol 14(6): e4962.

Type

Empty backbone

Use

Tags

His6-myc

Expression

Bacterial

Mutation

Promoter

lac

Availability

Academic Institutions and Nonprofits only

Enlarge
mEmerald-MyosinVIIA-C-14

Plasmid

#62762

Purpose

Localization: Cytoskeleton, C1 Cloning Vector, Excitation:487, Emission:509

Depositor

Michael Davidson

Insert

MyosinVIIA

Use

Tags

mEmerald

Expression

Mammalian

Mutation

none

Promoter

CMV

Availability

Industry, Academic Institutions, and Nonprofits
mEmerald-Inversin-C-18

Plasmid

#54131

Purpose

Localization: Cilium, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Insert

Inversin (INVS Human)

Use

Tags

mEmerald

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Industry, Academic Institutions, and Nonprofits
pAGM9121_Sce-Invertase 2 SP

Plasmid

#153493

Purpose

S. cerevisiae Invertase Signal Peptide Donor Plasmid

Depositor

Sylvestre Marillonnet

Article

Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

Insert

Invertase 2 SP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pTS1108_Tier3(Lenti_inverse)

Plasmid

#169663

Purpose

Tier-3 vector for stable integration of up to three cassettes via lentiviral transduction in inverted direction with polyA (PCMV-5'LTR-Psi-gag-env-A3-pA::A2-pA::A1-pA-WPRE-3'LTR)

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pFrt-invCAG-Luc

Plasmid

#63577

Purpose

Shuttle vector compatible with Flp-mediated (Flp-in) transgene integration that allows for conditional Luciferase expression upon integration. The vector contains an FRT site and two mutant loxP sites

Depositor

Ivo Huijbers

Article

Huijbers et al Nat Protoc. 2015 Nov;10(11):1755-85. doi

Insert

Stop-Lox71-Luciferase-pA-FRT-Lox66-reverseCAG

Use

Cre/Lox; Frt/flpe recombination mediated insertion

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pPTK013-3a-Invertase-αMFΔ

Plasmid

#84972

Purpose

Type 3a, Invertase followed by αMFΔ - secretion signal

Depositor

Volker Sieber

Article

Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

Insert

Invertase followed by αMFΔ secretion tag

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Non

Availability

Academic Institutions and Nonprofits only

Enlarge