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pT7-EGFP-C1-HsLSm1_J

Plasmid

#146676

Purpose

Mammalian Expression of HsLSm1

Depositor

Elisa Izaurralde

Article

Braun et al EMBO J. 2010 Jul 21. 29(14):2368-80.

Insert

HsLSm1 (LSM1 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pRSF_6His-ZmSUMO1a-GG_ZmSCE1b-Myc

Plasmid

#91932

Purpose

Bacterial expression of maize SUMO1a and SCE1b

Depositor

Richard Vierstra

Article

Augustine et al Plant Physiol. 2016 Jul;171(3):2191-210.

Inserts

6His-ZmSUMO1a-GG
ZmSCE1b-Myc

Tags

6His and Myc

Expression

Bacterial

Mutation

Truncation of ZmSUMO1a after the diGlycine motif

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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pCLC-mEos2

Plasmid

#38010

Depositor

Xiaowei Zhuang

Article

Jones et al Nat Methods. 2011 Jun;8(6):499-508. Epub

Insert

Clathrin, light polypeptide (LCa) (Clta Mouse)

Tags

mEos2

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Sept. 29, 2015

Availability

Academic Institutions and Nonprofits only

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hP14Gal4

Plasmid

#84364

Purpose

Gal4-PRDM14 funsion plasmid for luciferase recruitment assay

Depositor

Danny Reinberg

Article

Tu et al Nature. 2016 Jun 8;534(7607):387-90. doi

Insert

PRDM14 (PRDM14 Human)

Tags

Gal4 and HA tag

Expression

Mammalian

Mutation

silent mutations at coding silent 1068CtoT, 1629T…

Promoter

CMV/TO

Available Since

Feb. 6, 2017

Availability

Academic Institutions and Nonprofits only

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LR (bact: HyPer)

Plasmid

#162069

Purpose

Used for the diffuse visualization of H2O2 production in cells.

Depositor

Philipp Niethammer

Article

Enyedi et al Nat Cell Biol. 2013 Sep;15(9):1123-30. d

Insert

beta actin 2 (actb2 Zebrafish)

Use

Gateway donor vector

Tags

HyPer

Promoter

T3

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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p33Fn

Plasmid

#158155

Purpose

Expression of crRNA array and FnCas12a using one Pol II promoter. For plant genome editing.

Depositor

Kabin Xie

Article

Ding et al Mol Plant. 2018 Apr 2;11(4):542-552. doi

Type

Empty backbone

Use

CRISPR

Expression

Plant

Mutation

Rice codon optimized

Available Since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pRSF_6His-ZmSUMO1a-GG_ZmSCE1d-Myc

Plasmid

#91934

Purpose

Bacterial expression of maize SUMO1a and SCE1d

Depositor

Richard Vierstra

Article

Augustine et al Plant Physiol. 2016 Jul;171(3):2191-210.

Inserts

6His-ZmSUMO1a-GG
ZmSCE1d-Myc

Tags

6His and Myc

Expression

Bacterial

Mutation

Truncation of ZmSUMO1a after the diGlycine motif

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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pSpyTag-GlpG

Plasmid

#79054

Purpose

Expresses SpyTagged GlpG protein

Depositor

James Bowie

Article

Min et al Protein Sci. 2016 Aug;25(8):1535-44. doi

Insert

SpyTag-GlpG (glpG )

Tags

6xHis and SpyTag

Expression

Bacterial

Mutation

GlpG protein employed here corresponds to residue…

Promoter

Trc

Available Since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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pTX198

Plasmid

#89262

Purpose

Express STU Cas9 with Maize Ubiquitin1 promoter in rice targeting OsPDS gene, OsPDS-gRNA01

Depositor

Daniel Voytas

Article

Tang et al Mol Plant. 2016 Jul 6;9(7):1088-91. doi:

Insert

OsPDS-gRNA01

Use

CRISPR

Tags

SV40 NLS

Expression

Plant

Promoter

Maize Ubiquitin1 promoter

Available Since

May 5, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR221-OSF-TRIM5-21R

Plasmid

#79063

Purpose

Gateway entry vector for cloning and propagation in DH5alpha

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

OSF-TRIM5-21R

Tags

OneSTrEP-FLAG

Expression

Insect

Promoter

T7

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pET11a-CA(A14C, E45C, A92E)

Plasmid

#79046

Purpose

Bacterial vector for cloning and propagation in DH5alpha

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

CA (A14C, E45C, A92E)

Expression

Bacterial

Mutation

A14C, E45C, A92E mutations in HIV-1 CA protein

Promoter

T7

Available Since

March 22, 2017

Availability

Academic Institutions and Nonprofits only

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HGXPRT

Plasmid

#64201

Purpose

Expression of Plasmodium falciparum HGXPRT

Depositor

Vern Schramm

Article

Hazleton et al Chem Biol. 2012 Jun 22;19(6):721-30. doi

Insert

Hypoxanthine guanine xanthine phosphoribosyltransferase

Tags

Histidine tag

Expression

Bacterial

Available Since

April 29, 2015

Availability

Academic Institutions and Nonprofits only
pAct:dCas9-scFv

Plasmid

#78900

Purpose

Expresses scFv-VP64 under actin promoter for SunTag CRISPRa in Drosophila cells

Depositor

Norbert Perrimon

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

scFV-VP64

Use

CRISPR

Tags

GFP

Expression

Insect

Promoter

pActin (Drosophila)

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pGEX4T-1-human RNF146 RING-WWE (30-183)

Plasmid

#132611

Purpose

expresses human RNF146 RING-WWE (30-183) in E. coli

Depositor

Wenqing Xu

Article

DaRosa et al Nature. 2015 Jan 8;517(7533):223-6. doi:

Insert

RNF146 RING-WWE (30–183)

Expression

Bacterial

Available Since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pHiUGE Myc-epitope donor ORF0

Plasmid

#200374

Purpose

Tagging endogenously expressed proteins with Myc epitope, C-terminal

Depositor

Scott Soderling

Article

Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

Insert

Myc epitope

Expression

Mammalian

Mutation

NA

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pHiUGE Myc-epitope donor ORF1

Plasmid

#200375

Purpose

Tagging endogenously expressed proteins with Myc epitope, C-terminal

Depositor

Scott Soderling

Article

Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

Insert

Myc epitope

Expression

Mammalian

Mutation

NA

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pSiP1

Plasmid

#21952

Depositor

Klaus Rajewsky

Article

Xiao et al Nat Immunol. 2008 Apr . 9(4):405-14.

Insert

Pten (Pten Mouse)

Expression

Mammalian

Available Since

March 17, 2010

Availability

Academic Institutions and Nonprofits only
pET14_GFP5_Encap

Plasmid

#86408

Purpose

Expresses sfGFP tagged with a 5 amino acid CLP and encapsulin as an operon

Depositor

David Savage

Article

Cassidy-Amstutz et al Biochemistry. 2016 Jun 21;55(24):3461-8.

Inserts

sfGFP with a 5aa CLP tag
Encapsulin

Tags

6x Histidine

Expression

Bacterial

Promoter

T7

Available Since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

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pRK-flag-USP19 1-493

Plasmid

#78581

Purpose

Expression flag-USP19 1-493 in mammalian cells

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

Insert

flag-USP19 1-493 (USP19 Human)

Tags

flag

Expression

Mammalian

Mutation

mutated 494 residue to stop codon

Promoter

CMV

Available Since

June 15, 2016

Availability

Academic Institutions and Nonprofits only
pCK300

Plasmid

#87766

Purpose

empty control plasmid, three terminators to place inserts between for insulation, ampR, pBR322 origin, lacI

Depositor

John Heap

Article

Kelly et al ACS Synth Biol. 2016 Oct 21;5(10):1136-1

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

None

Available Since

May 1, 2017

Availability

Academic Institutions and Nonprofits only

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