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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 173,477 results for: addgene

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Showing: 2041 - 2060 of 173477 results

  1. pHEY1.1.0-gDNA

    Plasmid
    #112393
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor HEY1
    Depositor
    Kevin White
    Insert
    HEY1 (HEY1 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Dec. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112393-plasmid-map-sequence-id-230239

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  2. MAPK1

    Plasmid
    #73248
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    MAPK1 (MAPK1 Human)
    Tags
    His-6-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    April 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    73248-plasmid-map-sequence-id-137205

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  4. pLKO-Tet-On-HDAC1-shRNA2

    Plasmid
    #90241
    Purpose
    Lentivirus for expression of shRNA2 against human HDAC1 (Dox-inducible)
    Depositor
    Roland Friedel
    Insert
    HDAC1 (HDAC1 Human)
    Use
    Lentiviral
    Available Since
    May 17, 2017
    Availability
    Academic Institutions and Nonprofits only
    90241-plasmid-map-sequence-id-178296

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  5. pLKO-Tet-On-HDAC1-shRNA1

    Plasmid
    #90242
    Purpose
    Lentivirus for expression of shRNA1 against human HDAC1 (Dox-inducible)
    Depositor
    Roland Friedel
    Insert
    HDAC1 (HDAC1 Human)
    Use
    Lentiviral
    Available Since
    May 17, 2017
    Availability
    Academic Institutions and Nonprofits only
    90242-plasmid-map-sequence-id-178298

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  6. pLKO-Tet-On-HDAC1-shRNA3

    Plasmid
    #90243
    Purpose
    Lentivirus for expression of shRNA3 against human HDAC1 (Dox-inducible)
    Depositor
    Roland Friedel
    Insert
    HDAC1 (HDAC1 Human)
    Use
    Lentiviral
    Available Since
    May 17, 2017
    Availability
    Academic Institutions and Nonprofits only
    90243-plasmid-map-sequence-id-178297

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  7. pLenti-dCas9-KRAB-gRNA-TRE-blast

    Plasmid
    #201152
    Purpose
    Lentiviral expression of S. pyogenes dead Cas9 (dCas9/dSpCas9/SpdCas9) fused to the KRAB domain as well as a gRNA targeting the tight TRE promoter and the blasticidin resistance gene.
    Depositor
    Moshe Szyf
    Inserts
    dCas9-KRAB
    gRNA-TRE
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Mutation
    gRNA sequence: TACGTTCTCTATCACTGAT
    Available Since
    July 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    201152-plasmid-map-sequence-id-401799

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  11. pCJ1021

    Plasmid
    #228761
    Purpose
    Plasmid expressing Cas9 and 2 gRNAs for mouse Ift88. Use for disruption of mouse Ift88 in cultured cells.
    Depositor
    Bradley Yoder
    Insert
    Ift88 gRNAs (Ift88 Mouse)
    Use
    CRISPR
    Tags
    3XFLAG and GFP
    Expression
    Mammalian
    Promoter
    hU6
    Available Since
    Feb. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    228761-plasmid-map-sequence-id-455784

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  12. ACE2 g1 + Cas9

    Plasmid
    #153011
    Purpose
    A guide RNA targeting ACE2 in a lentiviral plasmid co-expressing Cas9 and TagBFP2
    Depositor
    Jason Sheltzer
    Insert
    Cas9 + ACE2 gRNA (ACE2 Human)
    Use
    CRISPR and Lentiviral
    Tags
    TagBFP2
    Available Since
    June 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    153011-plasmid-map-sequence-id-298673

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  17. pBRα

    Plasmid
    #53731
    Purpose
    Plpp/PlacUV5 -directed synthesis of E. coli RNAP alpha subunit; used as two-hybrid control. For cloning purposes, see Addgene plasmid 53734.
    Depositor
    Ann Hochschild
    Article
    Dove et al Nature. 1997 Apr 10;386(6625):627-30.
    Insert
    αNTD
    Expression
    Bacterial
    Promoter
    lpp/lacUV5 (tandem promoter)
    Available Since
    June 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    53731-plasmid-map-sequence-id-83909

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  18. ITCH

    Plasmid
    #36197
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    ITCH (ITCH Human)
    Tags
    His tag with TEV cleavage
    Expression
    Bacterial
    Mutation
    contains HECT domain
    Available Since
    June 19, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  20. p-dCas9-HDAC1-H141A-Hygro

    Plasmid
    #104417
    Purpose
    transient expression of dCas9-dHDAC1 fusion protein
    Depositor
    Stephan Beck
    Insert
    dHDAC1 (HDAC1 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    H141A
    Promoter
    CMV promoter
    Available Since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    104417-plasmid-map-sequence-id-204223

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Showing: 2041 - 2060 of 173477 results