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pLP-pTac-himar-apraR

Plasmid

#199995

Purpose

Landing Pad with T7 RNAP circuit variant T15. pIP1443 seed promoter. pTac-himar transposase. In dap auxotrophic conjugation donor. This material contains 2 plasmids. For more information look below.

Depositor

Farren Isaacs

Article

Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

Insert

Landing Pads

Expression

Bacterial

Mutation

himar frameshift

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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GL4.10 Prg4 promoter-luciferase

Plasmid

#195034

Purpose

Prg4 enhancer/promoterfirefly luciferase constructs were constructed by cloning various Prg4 regulatory fragments into pGL4.10[luc2] vector

Depositor

Andrew Lassar, Cheng-Hai Zhang

Article

Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

Insert

Prg4 promoter (PRG4 Bovine)

Use

Luciferase

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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185_pETcon_SARS2_BQ1-1

Plasmid

#208085

Purpose

yeast surface display of the SARS-CoV-2 BQ.1.1 variant RBD

Depositor

Tyler Starr

Article

Taylor et al PLoS Pathog. 2023 Dec 29;19(12):e1011901

Insert

SARS-CoV-2 BQ.1.1 RBD (S SARS-CoV-2 virus, Budding Yeast)

Tags

HA and c-Myc

Expression

Yeast

Available Since

Oct. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pEYFP-N1-HsSmg7-K66ER163E_S

Plasmid

#147479

Purpose

Mammalian Expression of HsSmg7-K66ER163E

Depositor

Elisa Izaurralde

Article

Fukuhara et al Mol Cell. 2005 Feb 18;17(4):537-47. doi:

Insert

HsSmg7-K66ER163E (SMG7 Human)

Expression

Mammalian

Mutation

one non silent mutation G2560A (V854I) and three …

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmGW182-delM2_H

Plasmid

#146521

Purpose

Insect Expression of DmGW182-delM2

Depositor

Elisa Izaurralde

Article

Eulalio et al RNA. 2009 Jun;15(6):1067-77. doi: 10.126

Insert

DmGW182-delM2 (gw Fly)

Expression

Insect

Mutation

Two non silent mutations V704A and N799S and one …

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmGW182-12xmut_F

Plasmid

#146320

Purpose

Insect Expression of DmGW182-12xmut

Depositor

Elisa Izaurralde

Article

Eulalio et al RNA. 2009 Jun;15(6):1067-77. doi: 10.126

Insert

DmGW182-12xmut (gw Fly)

Expression

Insect

Mutation

Two non silent mutations V704A and N799S and one …

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmGW182-12xmut_E

Plasmid

#146251

Purpose

Insect Expression of DmGW182-12xmut

Depositor

Elisa Izaurralde

Article

Eulalio et al RNA. 2009 Jun;15(6):1067-77. doi: 10.126

Insert

DmGW182-12xmut (gw Fly)

Expression

Insect

Mutation

Two non silent mutations V704A and N799S and one …

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmGe1_J

Plasmid

#146688

Purpose

Insect Expression of DmGe1

Depositor

Elisa Izaurralde

Article

Behm-Ansmant et al Genes Dev. 2006 Jul 15. 20(14):1885-98.

Insert

DmGe1 (Ge-1 Fly)

Expression

Insect

Mutation

two non silent mutations Q194R and I1008T compare…

Available Since

Jan. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pAFMW-Nbr[R280A,K284A]

Plasmid

#188598

Purpose

Flag-tagged Nibbler variants expression in Drosophila S2 cells

Depositor

Stefan Ameres

Article

Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Insert

Nibbler (Nbr Fly)

Tags

FLAG-Myc

Expression

Insect

Mutation

R280A [CGA=>GCA], K284A [AAG=> GCG]

Available Since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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LLP727_L2_pV01_0I_TMV-rLUC_TCTP-fLUC

Plasmid

#192370

Purpose

To control for variation introduced to the gene circuits, Rluc expression not affected by the introduction of terminators or recombination target sites.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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