We narrowed to 2,117 results for: Pam

1. DHN_VrDHN1a_mCh-SspB (pBS1074)

   Plasmid

   #185298

   Purpose

   Mammalian expression of riverbank grape plant protein DHN_VrDHN1a attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation.

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   PvLEA4_repeats_k3_26
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. ApoE3_D125I_47-300_mCh-SspB (pBS1071)

   Plasmid

   #185294

   Purpose

   For the mammalian expression of the human protein ApoE3_D125I_47-300 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   ApoE3_D125I_47-300
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D125I

   Promoter

   Available since

   July 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. ApoE3_D125I_mCh-SspB (pBS1145)

   Plasmid

   #185327

   Purpose

   For the mammalian expression of the human protein ApoE3_D125I attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   ApoE3_D125I
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D125I

   Promoter

   Available since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. ApoE4_mCh-SspB (pBS1143)

   Plasmid

   #185325

   Purpose

   For the mammalian expression of the human protein ApoE4 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   ApoE4
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. ApoE3_D125I_47-300_mCh-2xFKBP (pBS1114)

   Plasmid

   #185309

   Purpose

   For the mammalian expression of the human protein ApoE3_D125I_47-300 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   ApoE3_D125I_47-300
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D125I

   Promoter

   Available since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pvLEA22mer_mutant_3_mCh-SspB (pBS1080)

   Plasmid

   #185303

   Purpose

   For the mammalian expression of the sleeping chironomid protein pvLEA22mer_mutant_3 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   pvLEA22mer_mutant_3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. PvLEA4_repeats_k5_1_mCh-SspB (pBS1079)

   Plasmid

   #185302

   Purpose

   For the mammalian expression of the sleeping chironomid protein PvLEA4_repeats_k5_1 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   PvLEA4_repeats_k5_1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pvLEA22mer_mutant_10_mCh-SspB (pBS1078)

   Plasmid

   #185301

   Purpose

   For the mammalian expression of the sleeping chironomid protein pvLEA22mer_mutant_10 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   pvLEA22mer_mutant_10
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. CAHS2_PARRC_98-130_mCh-SspB (pBS1077)

   Plasmid

   #185300

   Purpose

   For the mammalian expression of the tardigrade protein CAHS2_PARRC_98-130 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   CAHS2_PARRC_98-130
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. DSUP_RAMVA_1-208_mCh-SspB (pBS1073)

    Plasmid

    #185296

    Purpose

    For the mammalian expression of the tardigrade protein DSUP_RAMVA_1-208 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    DSUP_RAMVA_1-208
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. DHR93_Nccapped_mCh-SspB (pBS1072)

    Plasmid

    #185295

    Purpose

    For the mammalian expression of the synthetic protein DHR93_Nccapped attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    DHR93_Nccapped
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. ApoE3_47-300_mCh-SspB (pBS1070)

    Plasmid

    #185293

    Purpose

    For the mammalian expression of the human protein ApoE3_47-300 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    ApoE3_47-300
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. ApoE4_47-300_mCh-SspB (pBS1069)

    Plasmid

    #185292

    Purpose

    For the mammalian expression of the human protein ApoE4_47-300 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    ApoE4_47-300
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. ApoE2_47-300_mCh-SspB (pBS1068)

    Plasmid

    #185291

    Purpose

    For the mammalian expression of the human protein ApoE2_47-300 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    ApoE2_47-300
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. LLPS2-CAHS2_PARRC::SspB (pBS1043)

    Plasmid

    #185290

    Purpose

    For the mammalian expression of the tardigrade protein CAHS2_PARRC attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    CAHS2_PARRC
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. LLPS1-FUSN:SspB (pBS1041)

    Plasmid

    #185288

    Purpose

    For the mammalian expression of the human protein FUSN attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    FUSN
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMCh-660

    Plasmid

    #92259

    Purpose

    Plasmid for expression of NHA-tagged human TNRC6C 7W7A CED with seven mutated W-motifs and mutated PAM2 motif in mammalian cells

    Depositor

    Witold Filipowicz, Marina Chekulaeva

    Article

    Chekulaeva et al Nat Struct Mol Biol. 2011 Oct 7;18(11):1

    Insert

    TNRC6C CED (TNRC6C Human)
    

    Use

    Tags

    NHA

    Expression

    Mammalian

    Mutation

    C-terminal effector domain (CED): aa 1369-1690 wi…

    Promoter

    CMV

    Available since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMCh-665

    Plasmid

    #92254

    Purpose

    Plasmid for expression of GST-tagged human TNRC6C 7W7A CED with seven mutated W-motifs and mutated PAM2 motif in mammalian cells

    Depositor

    Witold Filipowicz, Marina Chekulaeva

    Article

    Chekulaeva et al Nat Struct Mol Biol. 2011 Oct 7;18(11):1

    Insert

    TNRC6C CED (TNRC6C Human)
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    C-terminal effector domain (CED): aa 1369-1690 wi…

    Promoter

    EF1A

    Available since

    Oct. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pACEBac1-gamma-TuSC

    Plasmid

    #178079

    Purpose

    Insect cell expression of gamma-TuSC

    Depositor

    Tarun Kapoor

    Article

    Wieczorek et al J Cell Biol. 2021 Mar 1;220(3). pii: 211

    Inserts

    gamma-tubulin (TUBG1 Human)
    GCP2 (TUBGCP2 Human)
    GCP3 (TUBGCP3 Human)
    

    Use

    Tags

    TEV-His6

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAG-mCherry-FKBP-GEF(ITSN1)

    Plasmid

    #222631

    Purpose

    Cdc42 activation using rapamycin

    Depositor

    Arnold Hayer

    Article

    Marshall-Burghardt et al Sci Adv. 2024 Sep 6;10(36):eadn6858. doi

    Insert

    mCherry-FKBP-GEF(ITSN1) (ITSN1 Human)
    

    Use

    Tags

    mCherry-FKBP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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