Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 9,918 results for: codon optimized

Clear keywords
Showing: 2061 - 2080 of 9918 results

  3. iSpyMac

    Plasmid
    #155014
    Purpose
    Expresses the codon-optimized, engineered iSpyMac Cas9 nuclease in mammalian cells
    Depositor
    Joseph Jacobson
    Publication
    Chatterjee et al Nat Commun. 2020 May 18;11(1):2474. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    iSpyMac
    Expression
    Mammalian
    Mutation
    PID grafted onto SpyCas9 N-terminal domain, R221K…
    Promoter
    EF1ɑ
    Available since
    Sept. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    155014-plasmid-map-sequence-id-308820

    Enlarge

  4. iSpyMac

    Plasmid
    #155014
    Purpose
    Expresses the codon-optimized, engineered iSpyMac Cas9 nuclease in mammalian cells
    Depositor
    Joseph Jacobson
    Publication
    Chatterjee et al Nat Commun. 2020 May 18;11(1):2474. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    iSpyMac
    Expression
    Mammalian
    Mutation
    PID grafted onto SpyCas9 N-terminal domain, R221K…
    Promoter
    EF1ɑ
    Available since
    Sept. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    155014-plasmid-map-sequence-id-308820

    Enlarge

  5. C1-FT-mCitrine-EAAARx8-CaaX

    Plasmid
    #155224
    Purpose
    MPAct with increased search radius
    Depositor
    Tobias Meyer
    Publication
    Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    F-tractin (aa9-52 of rat ITPKA) fused to mCitrine C-term tagged with codon optimized (EAAAR)x8 and CaaX seq derived from Kras4b
    Tags
    mCitrine
    Expression
    Mammalian
    Available since
    Sept. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    155224-plasmid-map-sequence-id-310122

    Enlarge

  6. C1-FT-mCitrine-EAAARx8-CaaX

    Plasmid
    #155224
    Purpose
    MPAct with increased search radius
    Depositor
    Tobias Meyer
    Publication
    Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    F-tractin (aa9-52 of rat ITPKA) fused to mCitrine C-term tagged with codon optimized (EAAAR)x8 and CaaX seq derived from Kras4b
    Tags
    mCitrine
    Expression
    Mammalian
    Available since
    Sept. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    155224-plasmid-map-sequence-id-310122

    Enlarge

  7. pJM147

    Plasmid
    #134167
    Purpose
    Retroviral plasmid expressing GFP - Nde1
    Depositor
    Iain Cheeseman
    Publication
    Monda et al Mol Biol Cell. 2018 Sep 15;29(19):2336-2
    Insert
    Nde1_SSSC E47A E. coli codon optimized (CRISPR/Cas9 hardened) (NDE1 Human)
    Use
    Retroviral
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    SSSC E47A, E. coli codon optimized, CRISPR/Cas9 h…
    Available since
    Feb. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    134167-plasmid-map-sequence-id-271753

    Enlarge

  8. pJM147

    Plasmid
    #134167
    Purpose
    Retroviral plasmid expressing GFP - Nde1
    Depositor
    Iain Cheeseman
    Publication
    Monda et al Mol Biol Cell. 2018 Sep 15;29(19):2336-2
    Insert
    Nde1_SSSC E47A E. coli codon optimized (CRISPR/Cas9 hardened) (NDE1 Human)
    Use
    Retroviral
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    SSSC E47A, E. coli codon optimized, CRISPR/Cas9 h…
    Available since
    Feb. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    134167-plasmid-map-sequence-id-271753

    Enlarge

  10. pFS_0453_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)_T7term_ARRAY2_FaqI

    Plasmid
    #117006
    Purpose
    Expresses E. coli codon-optimized FsRT-Cas1-Cas2 under ptetA promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readout
    Depositor
    Randall Platt
    Publication
    Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:
    Insert
    Fusicatenibacter saccharivorans RT-Cas1-Cas2
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Nov. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    117006-plasmid-map-sequence-id-227036

    Enlarge

  12. pFS_0453_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)_T7term_ARRAY2_FaqI

    Plasmid
    #117006
    Purpose
    Expresses E. coli codon-optimized FsRT-Cas1-Cas2 under ptetA promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readout
    Depositor
    Randall Platt
    Publication
    Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:
    Insert
    Fusicatenibacter saccharivorans RT-Cas1-Cas2
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Nov. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    117006-plasmid-map-sequence-id-227036

    Enlarge

  14. pTUB1:OsTIR1-3FLAG, SAG1:CAT

    Plasmid
    #87258
    Purpose
    TIR1-3FLAG fusion driven by a TUB1 promoter with a CAT drug selectable marker. The TIR1 CDS from Oryza sativa was codon optimized for T. gondii expression.
    Depositor
    David Sibley
    Publication
    Brown et al MBio. 2017 May 2;8(3). pii: e00375-17. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    TIR1
    CAT
    Use
    Toxoplasma expression
    Tags
    3FLAG
    Mutation
    Codon optimized for Toxoplasma gondii expression
    Promoter
    TgSAG1 361 bp and TgTUB1 2717 bp
    Available since
    May 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    87258-plasmid-map-sequence-id-176556

    Enlarge

  16. pTUB1:OsTIR1-3FLAG, SAG1:CAT

    Plasmid
    #87258
    Purpose
    TIR1-3FLAG fusion driven by a TUB1 promoter with a CAT drug selectable marker. The TIR1 CDS from Oryza sativa was codon optimized for T. gondii expression.
    Depositor
    David Sibley
    Publication
    Brown et al MBio. 2017 May 2;8(3). pii: e00375-17. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    TIR1
    CAT
    Use
    Toxoplasma expression
    Tags
    3FLAG
    Mutation
    Codon optimized for Toxoplasma gondii expression
    Promoter
    TgSAG1 361 bp and TgTUB1 2717 bp
    Available since
    May 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    87258-plasmid-map-sequence-id-176556

    Enlarge

  17. pET-6His-MBP-MpAgo

    Plasmid
    #79249
    Purpose
    This plasmid encodes the Argonaute protein from Marinitoga Piezophila and is codon optimized for E. coli expression
    Depositor
    Jennifer Doudna
    Publication
    Kaya et al Proc Natl Acad Sci U S A. 2016 Mar 30. p
    Insert
    Marinitoga piezophila Argonaute
    Use
    CRISPR
    Tags
    6XHis-tag + MBP + PreScission Cleavage Site
    Expression
    Bacterial
    Promoter
    T7
    Available since
    July 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    79249-plasmid-map-sequence-id-151486

    Enlarge

  18. pET-6His-MBP-MpAgo

    Plasmid
    #79249
    Purpose
    This plasmid encodes the Argonaute protein from Marinitoga Piezophila and is codon optimized for E. coli expression
    Depositor
    Jennifer Doudna
    Publication
    Kaya et al Proc Natl Acad Sci U S A. 2016 Mar 30. p
    Insert
    Marinitoga piezophila Argonaute
    Use
    CRISPR
    Tags
    6XHis-tag + MBP + PreScission Cleavage Site
    Expression
    Bacterial
    Promoter
    T7
    Available since
    July 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    79249-plasmid-map-sequence-id-151486

    Enlarge

Showing: 2061 - 2080 of 9918 results