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Xalb_I-F1_array_GG

Plasmid

#178770

Purpose

Golden Gate vector to generate X. albilineans type I-F1 single arrays

Depositor

Chase Beisel

Publication

Wimmer et al Mol Cell. 2022 Feb 16. pii: S1097-2765(2

Insert

Golden Gate vector BaeI recognition site to generate X. albilineans type I-F1 single arrays

Expression

Bacterial

Available since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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Sth_I-E array_GFP dropout

Plasmid

#178775

Purpose

Golden Gate GFP dropout vector to generate S. thermophilus type I-E single arrays

Depositor

Chase Beisel

Publication

Wimmer et al Mol Cell. 2022 Feb 16. pii: S1097-2765(2

Insert

gfp dropout to generate S. thermophilus type I-E single arrays

Expression

Bacterial

Available since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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pXalb_IC_Cascade

Plasmid

#178765

Purpose

Expression of X. albilineans type I-C Cascade genes

Depositor

Chase Beisel

Publication

Wimmer et al Mol Cell. 2022 Feb 16. pii: S1097-2765(2

Insert

X. albilineans type I-C Cascade genes

Expression

Bacterial

Available since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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Xalb_I-C_array_GG

Plasmid

#178767

Purpose

Golden Gate vector to generate X. albilineans type I-C single arrays

Depositor

Chase Beisel

Publication

Wimmer et al Mol Cell. 2022 Feb 16. pii: S1097-2765(2

Insert

Golden Gate vector BaeI recognition site to generate X. albilineans type I-C single arrays

Expression

Bacterial

Available since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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pEc-crRNA2

Plasmid

#170089

Purpose

encodes E. coli type I-E single spacer array

Depositor

Chase Beisel

Publication

Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

Insert

E. coli type I-E single spacer array

Use

CRISPR and Synthetic Biology

Promoter

J23119

Available since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pCDH_CMV_HMGB_ctail_mKate2_CLEAVAGE

Plasmid

#167338

Purpose

Expresses mKate2 fused to TFAM HMGB+C-tail mutant in mammalian cells

Depositor

Tom Misteli

Publication

Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

Insert

TFAM (TFAM Human)

Use

Lentiviral

Tags

mKate2

Expression

Mammalian

Mutation

Truncation mutant containing MTS (1-42) as well a…

Promoter

CMV

Available since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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pCDH_CMV_L6_mKate2

Plasmid

#167332

Purpose

Expresses mKate2 fused to TFAM L6 mutant in mammalian cells

Depositor

Tom Misteli

Publication

Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

Insert

TFAM (TFAM Human)

Use

Lentiviral

Tags

mKate2

Expression

Mammalian

Mutation

Changed K136, H137, K139, R140, K146 and K147 to …

Promoter

CMV

Available since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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Human_TFAM_HMGB_ctail_pET28

Plasmid

#167330

Purpose

Expresses TFAM HMGB+C-tatil mutant in bacterial cells

Depositor

Tom Misteli

Publication

Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

Insert

TFAM (TFAM Human)

Tags

6XHis

Expression

Bacterial

Mutation

Truncation mutant containing HMGB and C-tail doma…

Promoter

T7

Available since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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pRS314-U6-G96C/A97C/U100C/U101C

Plasmid

#82328

Purpose

U6 telestem mutant (G96C/A97C/U100C/U101C) for in vivo studies

Depositor

Aaron Hoskins

Publication

Rodgers et al Nucleic Acids Res. 2016 Dec 15;44(22):10

Insert

U6 RNA with G96C/A97C/U100C/U101C mutation (snR6 Budding Yeast)

Expression

Yeast

Mutation

U6 telestem mutant (A34G/A35G/G96C/A97C/U100C/U10…

Available since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pRS314_U6_A34G_A35G

Plasmid

#81232

Purpose

U6 telestem mutant (A34G/A35G) for in vivo studies

Depositor

Aaron Hoskins

Publication

Rodgers et al Nucleic Acids Res. 2016 Dec 15;44(22):10

Insert

U6 RNA with A34G/A35G mutation (snR6 Budding Yeast)

Expression

Yeast

Mutation

U6 telestem mutant (A34G/A35G)

Available since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pRS314_U6_G96C_A97C

Plasmid

#81235

Purpose

U6 telestem mutant (G96C/A97C) for in vivo studies

Depositor

Aaron Hoskins

Publication

Rodgers et al Nucleic Acids Res. 2016 Dec 15;44(22):10

Insert

U6 RNA with G96C/A97C mutation (snR6 Budding Yeast)

Expression

Yeast

Mutation

U6 telestem mutant (G96C/A97C)

Available since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pRS314_U6_U100C_U101C

Plasmid

#81236

Purpose

U6 telestem mutant (U100C/U101C) for in vivo studies

Depositor

Aaron Hoskins

Publication

Rodgers et al Nucleic Acids Res. 2016 Dec 15;44(22):10

Insert

U6 RNA with U100C_U101C mutation (snR6 Budding Yeast)

Expression

Yeast

Mutation

U6 telestem mutant (G96C/A97C)

Available since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pRS314-U6-A34G/A35G/G96C/A97C

Plasmid

#81238

Purpose

U6 telestem mutant (A34G/A35G/G96C/A97C) for in vivo studies

Depositor

Aaron Hoskins

Publication

Rodgers et al Nucleic Acids Res. 2016 Dec 15;44(22):10

Insert

U6 RNA with A34G/A35G/G96C/A97C mutation (snR6 Budding Yeast)

Expression

Yeast

Mutation

U6 telestem mutant (A34G/A35G/G96C/A97C)

Available since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pTK660

Plasmid

#114702

Purpose

Express NuMA(1-705)4A-RFP-Nano from Rosa 26 locus

Depositor

Tomomi Kiyomitsu

Publication

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

NuMA(1-705)4A-RFP-Nano

Available since

Oct. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pTK525

Plasmid

#114691

Purpose

sgRNA for p150's C-terminal locus

Depositor

Tomomi Kiyomitsu

Publication

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

p150 sgRNA

Available since

Oct. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pXY029 (mEos2-MTSBs)

Plasmid

#72652

Purpose

Inducible expression of mEos2-MTSBs in bacteria

Depositor

Jie Xiao

Publication

Buss et al PLoS Genet. 2015 Apr 7;11(4):e1005128. d

Insert

mEos2-MTSBs

Tags

mEos2

Expression

Bacterial

Promoter

T5-lac

Available since

Feb. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pJB051 (mEos2-ZapA)

Plasmid

#72650

Purpose

Inducible expression of mEos2-ZapA in bacteria

Depositor

Jie Xiao

Publication

Buss et al PLoS Genet. 2015 Apr 7;11(4):e1005128. d

Insert

mEos2-ZapA

Tags

mEos2

Expression

Bacterial

Promoter

T5-lac

Available since

Feb. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pAN-PBAD-sgRNA-VR

Plasmid

#62286

Purpose

expression of VR sgRNA from the arabinose-inducible promoter

Depositor

Christopher Voigt

Publication

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Insert

VR sgRNA

Use

CRISPR

Expression

Bacterial

Promoter

pBAD

Available since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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pAN-PBAD-sgRNA-A5T

Plasmid

#62264

Purpose

expression of A5T sgRNA from the arabinose-inducible promoter

Depositor

Christopher Voigt

Publication

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Insert

A5T sgRNA

Use

CRISPR

Expression

Bacterial

Promoter

pBAD

Available since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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pAN-PBAD-sgRNA-A5NT

Plasmid

#62263

Purpose

expression of A5NT sgRNA from the arabinose-inducible promoter

Depositor

Christopher Voigt

Publication

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Insert

A5NT sgRNA

Use

CRISPR

Expression

Bacterial

Promoter

pBAD

Available since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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