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HK2 gRNA (BRDN0001162236)

Plasmid

#76309

Purpose

3rd generation lentiviral gRNA plasmid targeting human HK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

HK2 (HK2, HOOK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR223_ABCB9_WT

Plasmid

#82899

Purpose

Gateway Donor vector containing ABCB9, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

ABCB9 (ABCB9 Human)

Use

Gateway entry vector

Mutation

582_590delISLVSQEPVinsVCARAWATL; 592_595delFARSin…

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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IMPT-10917

Plasmid

#236192

Purpose

Express GPR6 in insect cells

Depositor

Vadim Cherezov

Article

Barekatain et al Sci Signal. 2024 Dec 3;17(865):eado8741.

Insert

GPR6 (GPR6 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-MCP-Lbr-V5-mCherry

Plasmid

#235093

Purpose

Lbr mCherry fusion protein with MCP domain

Depositor

Mitchell Guttman

Article

Bhat et al Nature. 2024 May;629(8014):1165-1173. do

Insert

pCAG-MCP-Lbr-V5-mCherry (LBR Human)

Expression

Mammalian

Promoter

CAG

Available Since

May 7, 2025

Availability

Academic Institutions and Nonprofits only

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pdest METTL1-S27A Neo

Plasmid

#223059

Purpose

METTL1-S27A gene expression in mammalian cells

Depositor

Alejandro Gutierrez

Article

Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

Insert

METTL1-S27A (METTL1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

S27A

Promoter

CMV

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pdest METTL1-S27D Neo

Plasmid

#223060

Purpose

METTL1-S27D gene expression in mammalian cells

Depositor

Alejandro Gutierrez

Article

Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

Insert

METTL1-S27D (METTL1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

S27D

Promoter

CMV

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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rag2:METTL1-S27A_CE_pISceI

Plasmid

#223048

Purpose

Express METTL1-S27A gene in mesenchymal lineage of Zebrafish.

Depositor

Alejandro Gutierrez

Article

Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

Insert

METTL1-S27A (METTL1 Human)

Use

Express the insert(gene) in mesenchymal lineage o…

Mutation

S27A

Promoter

Rag2

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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rag2:METTL1-S27D_CE_pISceI

Plasmid

#223049

Purpose

Express METTL1-S27D gene in mesenchymal lineage of Zebrafish.

Depositor

Alejandro Gutierrez

Article

Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

Insert

METTL1-S27D (METTL1 Human)

Use

Express the insert(gene) in mesenchymal lineage o…

Mutation

S27D

Promoter

Rag2

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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rag2:METTL1-CM_CE_pISceI

Plasmid

#223050

Purpose

Express METTL1-CM gene in mesenchymal lineage of Zebrafish.

Depositor

Alejandro Gutierrez

Article

Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

Insert

METTL1-CM (METTL1 Human)

Use

Express the insert(gene) in mesenchymal lineage o…

Mutation

160-163 (LFPD>AFPA)

Promoter

Rag2

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pPICZ A-SLC[∆2-83]-His+MFN2-Strep (SB259)

Plasmid

#227608

Purpose

Inducible coexpression of His-tagged SLC25A46 in its N-terminal region (∆2-83) and Strep-tagged MFN2 in Pichia pastoris

Depositor

Luke Chao

Article

Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

Inserts

SLC25A46 (SLC25A46 Human)
MFN2 (MFN2 Human)

Tags

10xHis and Twin-StrepTag

Expression

Yeast

Mutation

aa 2-83 deleted

Promoter

AOX1

Available Since

Dec. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pPICZ A-SLC-His+MFN2-Strep (SB255)

Plasmid

#227604

Purpose

Inducible coexpression of His-tagged SLC25A46 and Strep-tagged MFN2 in Pichia pastoris

Depositor

Luke Chao

Article

Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

Inserts

SLC25A46 (SLC25A46 Human)
MFN2 (MFN2 Human)

Tags

10xHis and Twin-StrepTag

Expression

Yeast

Promoter

AOX1

Available Since

Dec. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pBIG1a_StrepII-Hook2_FTS_FHIP2A

Plasmid

#222302

Purpose

Co-expresses full-length human FHF (FTS, StrepII-HOOK2 and FHIP2A) in a pBIG1a vector

Depositor

Andrew Carter

Article

Abid Ali et al Nat Struct Mol Biol. 2025 Apr;32(4):756-

Inserts

HOOK2 (HOOK2 Human)
FTS (AKTIP Human)
FHIP2A (FHIP2A Human)

Tags

StrepII-Psc

Expression

Insect

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-Lgi1 [N283/7R-2b]

Plasmid

#222172

Purpose

Mammalian Expression Plasmid of anti-Lgi1 (Mouse). Derived from hybridoma N283/7.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-Lgi1 (Mus musculus) recombinant mouse monoclonal antibody. (Lgi1 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pCDH-V1-rCD20-BSD

Plasmid

#209752

Purpose

To reconstitute CD20 expression with a specific mRNA isoform (variant 1) after CRISPR-Cas9-mediated knockout of CD20.

Depositor

Andrei Thomas-Tikhonenko

Article

Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

Insert

MS4A1 (MS4A1 Human)

Use

Lentiviral

Mutation

The CCTGGGGGGTCTTCTGATGATCC sequence within the C…

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pCDH-V2-rCD20-BSD

Plasmid

#209753

Purpose

To reconstitute CD20 expression with a specific mRNA isoform (variant 2) after CRISPR-Cas9-mediated knockout of CD20.

Depositor

Andrei Thomas-Tikhonenko

Article

Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

Insert

MS4A1 (MS4A1 Human)

Use

Lentiviral

Mutation

The CCTGGGGGGTCTTCTGATGATCC sequence within the C…

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pCDH-V3-rCD20-BSD

Plasmid

#209754

Purpose

To reconstitute CD20 expression with a specific mRNA isoform (variant 3) after CRISPR-Cas9-mediated knockout of CD20.

Depositor

Andrei Thomas-Tikhonenko

Article

Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

Insert

MS4A1 (MS4A1 Human)

Use

Lentiviral

Mutation

The CCTGGGGGGTCTTCTGATGATCC sequence within the C…

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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MDM4_Deletion_Upstream_gRNA_2

Plasmid

#195135

Purpose

gRNA in a third generation Cas9 vector with GFP, targeting region immediately upstream of MDM4, to be used with MDM4_Deletion_Downstream_gRNA_1/2 for MDM4 deletion

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

MDM4 Deletion Upstream gRNA 2 (MDM4 Human)

Expression

Mammalian

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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MDM4_Deletion_Upstream_gRNA_1

Plasmid

#195134

Purpose

gRNA in a third generation Cas9 vector with GFP, targeting region immediately upstream of MDM4, to be used with MDM4_Deletion_Downstream_gRNA_1/2 for MDM4 deletion

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

MDM4 Deletion Upstream gRNA 1 (MDM4 Human)

Expression

Mammalian

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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eIF3a- pMK293 (mAID-mCherry2-Hygro) plasmid

Plasmid

#192236

Purpose

knockin donor vector of mAID-mCherry2-Hygro to C-terminus of endogenous human eIF3a

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

eIF3a-knockin-homology arm-mAID-mCherry2-Hygro (EIF3A Human)

Expression

Mammalian

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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eIF3a- pMK289 (mAID-mClover-NeoR) plasmid

Plasmid

#192235

Purpose

knockin donor vector of mAID-mClover-NeoR to C-terminus of endogenous human eIF3a

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

eIF3a-knockin-homology arm-mAID-mClover-NeoR (EIF3A Human)

Expression

Mammalian

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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