Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 18,031 results for: Mut

Showing: 2081 - 2100 of 18031 results

  1. pCEP4-Q842-QES.i04.c05-754*

    Plasmid
    #123232
    Purpose
    Mammalian expression plasmid for Env from the Q842.d12 HIV-1 isolate; C-terminal truncation; QES mutant with enhanced binding to PG16
    Depositor
    Erik Procko
    Article
    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.
    Insert
    HIV-1 (Q842.d12) Env
    Use
    Tags
    CD5 leader peptide
    Expression
    Mammalian
    Mutation
    Codon-optimized synthetic gene; Premature stop co…
    Promoter
    CMV
    Available Since
    March 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    123232-plasmid-map-sequence-id-235179

    Enlarge

  4. Ultra-Exon1Q145-Myc-A

    Plasmid
    #110487
    Purpose
    Lentiviral vector to express mutant N terminal HTT gene with short 3'UTR in mammalian cells (encodes Myc-tagged mutant huntingtin exon1 fragment with 145 repeats of Q)
    Depositor
    Baoji Xu
    Article
    Xu et al PLoS One. 2017 May 11;12(5):e0177610. do
    Insert
    Homo sapiens huntingtin (HTT Human)
    Use
    Lentiviral
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Partial long 3'UTR wild type huntingtin exon…
    Promoter
    UbC
    Available Since
    July 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    110487-plasmid-map-sequence-id-212608

    Enlarge

  6. pαH-S-GSAS/D614G

    Plasmid
    #164566
    Purpose
    SARS-CoV-2 Spike protein with D614G mutation (S-GSAS-D614G variant)
    Depositor
    Priyamvada Acharya
    Article
    Gobeil et al Cell Rep. 2021 Jan 12;34(2):108630. doi:
    Insert
    Spike (S-GSAS-D614G variant)
    Use
    Tags
    2X Strep-Tag II, 8X His tag, and HRV3C cleavage s…
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Promoter
    Available Since
    Feb. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    164566-plasmid-map-sequence-id-324830

    Enlarge

  9. pL452-Sf3b1-K700E

    Plasmid
    #90425
    Purpose
    selectable HDR vector to introduce K700E mutation within mus Sf3b1 gene (for use with sgSf3b1(T1) and pL452(hygro)-Sf3b1-K700K plasmids enabling generation of hemizygous K700E mutation)
    Depositor
    Alex Minella
    Article
    Gupta et al Nucleic Acids Res. 2018 Nov 20. pii: 519
    Insert
    Sf3b1 - left and right homology arms (Sf3b1 Mouse)
    Use
    Homology-directed repair vector
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    90425-plasmid-map-sequence-id-182684

    Enlarge

  10. pCAGGS-RaichuEV-Rac1(Y40C)

    Plasmid
    #164903
    Purpose
    Expresses a Rac1 FRET sensor RaichuEV-Rac1 which contains a dominant negative mutation Y40C in the Rac1 domain. More useful than T17N mutation due to less harmful to endogenous signaling.
    Depositor
    Takeshi Imai
    Article
    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi
    Insert
    RaichuEV-Rac1 (RAC1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Y40C mutation in the Rac1 domain whcih make the s…
    Promoter
    Available Since
    Aug. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    164903-plasmid-map-sequence-id-340567

    Enlarge

  12. pUltra-Exon1Q145-Myc-B

    Plasmid
    #110489
    Purpose
    Lentiviral vector to express mutant N terminal HTT gene with partial long 3'UTR in mammalian cells (encodes Myc-tagged mutant huntingtin exon1 fragment with 145 repeats of Q)
    Depositor
    Baoji Xu
    Article
    Xu et al PLoS One. 2017 May 11;12(5):e0177610. do
    Insert
    Homo sapiens huntingtin (HTT Human)
    Use
    Lentiviral
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    partial long 3'UTR wild type huntingtin exon…
    Promoter
    UbC
    Available Since
    July 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    110489-plasmid-map-sequence-id-287962

    Enlarge

  15. pCW57.1_SPTLC1_C133W

    Plasmid
    #181921
    Purpose
    3rd generation, Inducible lentiviral plasmid for expression of human mutant SPTLC1 C133W
    Depositor
    Christian Metallo
    Article
    Cordes et al J Lipid Res. 2022 Sep 14:100281. doi: 10
    Insert
    Human serine palmitoyltransferase, long chain base subunit 1 (SPTLC1) mutant C133W (SPTLC1 Human)
    Use
    Lentiviral; Mammalian expression, , doxycycline i…
    Tags
    Expression
    Mammalian
    Mutation
    cysteine at position 133 of the coding region of …
    Promoter
    tight TRE promoter
    Available Since
    May 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    181921-plasmid-map-sequence-id-361675

    Enlarge

  19. pCAGGS-SARS2-S-FKO (NC-flag)

    Plasmid
    #159363
    Purpose
    Mammalian expression of SARS-CoV-2 S protein (FKO: furin-cleavage site mutant) with the flag tag at N- and C-termini.
    Depositor
    Hyeryun Choe, Michael Farzan
    Article
    Zhang et al Nat Commun. 2020 Nov 26;11(1):6013. doi:
    Insert
    SARS-CoV-2 S protein (S SARS-CoV-2)
    Use
    Tags
    Flag and Flag
    Expression
    Mammalian
    Mutation
    Furin-cleavage site mutation
    Promoter
    CMV
    Available Since
    Sept. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    159363-plasmid-map-sequence-id-310910

    Enlarge

  20. Cav2.1 EA2, R57A, R59A pMT2

    Plasmid
    #206122
    Purpose
    expression of rabbit Cav2.1 calcium channel with a CaV2.1-P1217fs mutation causing a truncated protein, (EA2). R57A R59A mutations prevent dominant-negative suppression of Cav2.1 currents
    Depositor
    Annette Dolphin
    Article
    Dahimene et al Neurobiol Dis. 2016 Sep;93:243-56. doi:
    Insert
    cacna1a (Cacna1a Rat)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Cav2.1-P1217fs, R57A, R59A
    Promoter
    Ad MLP/TPL/SV40
    Available Since
    Nov. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    206122-plasmid-map-sequence-id-415911

    Enlarge

Showing: 2081 - 2100 of 18031 results