We narrowed to 11,256 results for: NSI;

1. C39m

   Plasmid

   #120972

   Purpose

   MoClo golden gate assembly CD part for ccdB (DNA gyrase toxin, modified synonymously to remove BsaI cut site; ccdA/C79m is antitoxin). Please see Supplemental Documents for annotated Genbank file.

   Depositor

   Richard Murray

   Insert

   ccdB toxin
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI site removed

   Promoter

   Available since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pME.C (pDHf14)

   Plasmid

   #24572

   Depositor

   David Pulleyblank

   Article

   Haniford et al Nature. 1983 Apr 14. 302(5909):632-4.

   Insert

   
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 3, 2010

   Availability

   Academic Institutions and Nonprofits only

3. p2090 HPV-11 E2

   Plasmid

   #10874

   Depositor

   Peter Howley

   Article

   Del Vecchio et al J Virol. 1992 Oct . 66(10):5949-58.

   Insert

   HPV11 E2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 26, 2005

   Availability

   Academic Institutions and Nonprofits only

4. C67m

   Plasmid

   #120981

   Purpose

   MoClo golden gate assembly CD part for Bxb1-mScarleti-LAA (Bxb1 integrase tagged with an mScarlet-I reporter and an LAA degradation tag). Please see Supplemental Documents for annotated Genbank file.

   Depositor

   Richard Murray

   Insert

   Bxb1 integrase - mScarlet-i - LAA tag
   

   Use

   Synthetic Biology

   Tags

   ssrA degradation tag

   Expression

   Mutation

   Promoter

   Available since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGL3_THADA_allscramble

   Plasmid

   #232386

   Purpose

   THADA enhancer, all sensitizing elements scrambled

   Depositor

   Joanna Wysocka

   Insert

   THADA (THADA Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   THADA enhancer, all sensitizing elements scrambled

   Promoter

   Available since

   Feb. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBP-P_TALEsp1_PUPsp1-lacI_Ptac-lacO

   Plasmid

   #204079

   Purpose

   Type 0 promoter part

   Depositor

   Mikhail Shapiro

   Insert

   TALEsp1-stabilized promoter driving lacI repressor; Ptac-lacO IPTG-inducible promoter
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   None

   Promoter

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. UC16m

   Plasmid

   #121038

   Purpose

   MoClo golden gate assembly DE part for gQi gRNA (guide RNA for S. pyogenes Cas9; sequence from DOI: 10.1016/j.cell.2013.02.022). Please see Supplemental Documents for annotated Genbank file.

   Depositor

   Richard Murray

   Insert

   gQi Cas9 gRNA UC part
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. UC17m

   Plasmid

   #121039

   Purpose

   MoClo golden gate assembly DE part for gV1 gRNA (guide RNA for S. pyogenes Cas9; sequence from doi: [10.15252/msb.20145735]). Please see Supplemental Documents for annotated Genbank file.

   Depositor

   Richard Murray

   Insert

   gV1 Cas9 gRNA UC part
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. C109m

   Plasmid

   #121011

   Purpose

   MoClo golden gate assembly CD part for mfLon-pdt3 (mfLon protease with self-targeting degradation tag pdt3). Please see Supplemental Documents for annotated Genbank file.

   Depositor

   Richard Murray

   Insert

   mfLon protease with pdt3 tag
   

   Use

   Synthetic Biology

   Tags

   pdt3 mfLon degradation tag

   Expression

   Mutation

   Promoter

   Available since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. C105m

    Plasmid

    #121008

    Purpose

    MoClo golden gate assembly CD part for sfYFP-AAV (superfolder YFP with AAV degradation tag for ssrA). Please see Supplemental Documents for annotated Genbank file.

    Depositor

    Richard Murray

    Insert

    sfYFP-AAV ssrA (has bbsI)
    

    Use

    Synthetic Biology

    Tags

    AAV degradation tag for ssrA

    Expression

    Mutation

    Promoter

    Available since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. C106m

    Plasmid

    #121009

    Purpose

    MoClo golden gate assembly CD part for sfYFP-DAS (superfolder YFP with DAS degradation tag for ssrA). Please see Supplemental Documents for annotated Genbank file.

    Depositor

    Richard Murray

    Insert

    sfYFP-DAS ssrA (has bbsI)
    

    Use

    Synthetic Biology

    Tags

    DAS degradation tag for ssrA

    Expression

    Mutation

    Promoter

    Available since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. C108m

    Plasmid

    #121010

    Purpose

    MoClo golden gate assembly CD part for mScarlet-I-pdt3 (fast-folding mScarlet variant, codon-optimized for E. col, with pdt3 degradation tag; see https://www.fpbase.org/protein/mscarlet-i/).

    Depositor

    Richard Murray

    Insert

    mScarlet-I codon optimized with pdt3 tag
    

    Use

    Synthetic Biology

    Tags

    pdt3 mfLon degradation tag

    Expression

    Mutation

    Promoter

    Available since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. C103m

    Plasmid

    #121007

    Purpose

    MoClo golden gate assembly CD part for mScarlet-I-LAA (fast-folding mScarlet variant, codon-optimized for E. col, with LAA degradation tag; see https://www.fpbase.org/protein/mscarlet-i/).

    Depositor

    Richard Murray

    Insert

    mScarlet-I codon optimized with LAA tag
    

    Use

    Synthetic Biology

    Tags

    LAA degradation tag for ssrA

    Expression

    Mutation

    Promoter

    Available since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. P35m

    Plasmid

    #120946

    Purpose

    MoClo golden gate assembly AB part for pLbda-CasRQi (strong lambda promoter with downstream dCas binding site for Qi-lab gRNA (UC16m); gRNA sequence taken from DOI: 10.1016/j.cell.2013.02.022).

    Depositor

    Richard Murray

    Insert

    pLbda-CasRQi
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCI_FLAG-GFP(Y39TAG)-6His

    Plasmid

    #223507

    Purpose

    Fluorescent reporter plasmid for genetic code expansion which consists of a modified GFP with a Amber stop codon on position Y39

    Depositor

    Edward Lemke

    Insert

    GFP(Y39TAG)
    

    Use

    Tags

    6xHis and FLAG

    Expression

    Mammalian

    Mutation

    Y39TAG

    Promoter

    Available since

    Oct. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGEX-4T-1-3xHA

    Plasmid

    #129567

    Purpose

    Empty backbone for HA tag

    Depositor

    Kevin Janes

    Article

    Smolko et al Sci Rep. 2019 Dec 19;9(1):19409. doi: 10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Bsrs078-LasR

    Plasmid

    #85147

    Purpose

    Constitutively expresses LasR

    Depositor

    Jeff Hasty

    Article

    Scott et al ACS Synth Biol. 2016 Sep 16;5(9):969-77.

    Insert

    LasR
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Bsrs078-TraR(W)

    Plasmid

    #85151

    Purpose

    Constitutively expresses TraR(W)

    Depositor

    Jeff Hasty

    Article

    Scott et al ACS Synth Biol. 2016 Sep 16;5(9):969-77.

    Insert

    TraR(W)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Bsrs078-LuxR

    Plasmid

    #85142

    Purpose

    Constitutively expresses LuxR

    Depositor

    Jeff Hasty

    Article

    Scott et al ACS Synth Biol. 2016 Sep 16;5(9):969-77.

    Insert

    LuxR
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMSP12::Kaede

    Plasmid

    #30172

    Depositor

    Lalita Ramakrishnan

    Article

    Davis et al Cell. 2009 Jan 9. 136(1):37-49.

    Insert

    Mycobacterium Strong Promoter (MSP)
    

    Use

    Mycobacteria expression

    Tags

    Kaede

    Expression

    Mutation

    Promoter

    Available since

    June 3, 2011

    Availability

    Academic Institutions and Nonprofits only