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pDONR223 SARS-CoV-2 NSP9

Plasmid

#141263

Purpose

Gateway-compatible Entry vector, with insert of NSP9 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP9 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available Since

April 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR223_KRAS_p.G12D

Plasmid

#81651

Purpose

Gateway Donor vector containing KRAS, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

KRAS (KRAS Human)

Use

Gateway Cloning

Mutation

G12D

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pET His6 Gamma crystallin TEV LIC cloning vector (1X)

Plasmid

#29661

Depositor

Scott Gradia

Type

Empty backbone

Tags

6xHis, Gamma-crystallin, and TEV cleavage site

Expression

Bacterial

Promoter

T7-lacO (lactose/IPTG inducible)

Available Since

June 7, 2011

Availability

Academic Institutions and Nonprofits only

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pCDB302

Plasmid

#113673

Purpose

expresses Cth SUMO protease in Escherichia coli

Depositor

Christopher Bahl

Article

Lau et al J Biol Chem. 2018 Aug 24;293(34):13224-1

Insert

Cth SUMO protease

Tags

10-His

Expression

Bacterial

Mutation

deleted amino acids 1-934

Promoter

T7

Available Since

Sept. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pQCXIH CMV/TO DEST (w382-1)

Plasmid

#17394

Purpose

Retroviral Gateway destination vector, Expression, CMV/TO promoter, Hygro

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Gateway Cloning and Retroviral

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pBITE-CD19-CD3

Plasmid

#190677

Purpose

Encodes a modularized form of HD37-OKT3 bispecific antibody

Depositor

Scott McComb

Article

Shepherd et al PLoS One. 2023 Jun 22;18(6):e0273884. do

Insert

Modularized-HD37-modularized-OKT3-P2A-NeonGreen

Use

Lentiviral

Tags

mNeonGreen

Expression

Mammalian

Promoter

EFS

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/Myc-His(-)-HSulf-1

Plasmid

#13003

Depositor

Steven Rosen

Article

Morimoto-Tomita et al J Biol Chem. 2002 Dec 20. 277(51):49175-

Insert

human sulfatase 1 (SULF1 Human)

Expression

Mammalian

Available Since

Oct. 30, 2006

Availability

Academic Institutions and Nonprofits only

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pICH41780

Plasmid

#48019

Depositor

Sylvestre Marillonnet

Article

Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

Type

Empty backbone

Use

Unspecified

Available Since

Dec. 3, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pOPINJ

Plasmid

#26045

Depositor

Ray Owens

Article

Berrow et al Nucleic Acids Res. 2007. 35(6):e45.

Insert

HisGST3C-lacZ

Tags

MAHHHHHH_GST_LEVLFQGT

Expression

Bacterial, Insect, and Mamm…

Available Since

Oct. 19, 2011

Availability

Academic Institutions and Nonprofits only

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pNIC28-DENV3-NS5FL

Plasmid

#86013

Purpose

Expression of dengue virus 3 NS5 protein

Depositor

Dahai Luo

Article

Zhao et al PLoS Pathog. 2015 Mar 16;11(3):e1004682.

Insert

DENV3-NS5FL

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

amino acid residues 6 to 895 of DENV3 NS5

Promoter

T7

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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pHW393 (Prab-3::GAL4-SK(DBD)::VP64::let-858 3'UTR)

Plasmid

#85583

Purpose

cGAL driver (Prab-3::GAL4-SK(DBD)::VP64::let-858 3'UTR) construct for C. elegans using the S. kudriavzevii (SK) GAL4 DNA-binding domain (DBD)

Depositor

Paul Sternberg

Article

Wang et al Nat Methods. 2016 Dec 19. doi: 10.1038/n

Insert

Prab-3-GAL4-SK(DBD)-VP64

Expression

Worm

Promoter

Prab-3-GAL4-SK(DBD)-VP64

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pMM290

Plasmid

#46038

Depositor

Raymond Monnat

Article

Swanson et al DNA Repair (Amst). 2004 May 4;3(5):475-8

Insert

WRN (WRN Human)

Use

Tre, bidirectional promoter

Tags

5x myc

Expression

Mammalian

Mutation

S2G introduced during cloning

Promoter

CMV

Available Since

May 29, 2014

Availability

Academic Institutions and Nonprofits only

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pKOR1

Plasmid

#133446

Purpose

Escherichia coli/S. aureus shuttle vector, permits rapid cloning via lambda recombination and ccdB selection

Depositor

Taeok Bae, Olaf Schneewind

Article

Bae et al Plasmid. 2006 Jan;55(1):58-63. doi: 10.1

Type

Empty backbone

Use

E.coli/s.aureus shuttle vector

Available Since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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HA-14-3-3 zeta

Plasmid

#116888

Purpose

Expresses HA-tagged human 14-3-3 zeta in mammalian cells

Depositor

FENG-QIAN Li, Ken-Ichi Takemaru

Article

Li et al J Cell Biol. 2008 Jun 30;181(7):1141-54.

Insert

14-3-3 zeta (YWHAZ Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

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RN3P-KDM5B-FLAG

Plasmid

#86398

Purpose

FLAG-tagged mouse KDM5B cDNA clone for mammalian cell expression

Depositor

Wei Xie

Article

Zhang et al Nature. 2016 Sep 14;537(7621):553-557. d

Insert

KDM5B (Kdm5b Mouse)

Tags

3x-FLAG

Expression

Mammalian

Promoter

Lac

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_SUV39H1_WT

Plasmid

#82236

Purpose

Gateway Donor vector containing SUV39H1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SUV39H1 (SUV39H1 Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-SomaGCaMP7

Plasmid

#158759

Purpose

Cell body-targeted GCaMP7f, under synapsin promoter: GCaMP7f followed by a linker, and the EE-RR coiled coil motif (GCaMP7f-27-EE-RR). As bright as conventional GCaMP7f.

Depositor

Edward Boyden

Article

Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)

Insert

SomaGCaMP7

Use

AAV

Expression

Mammalian

Promoter

synapsin promoter

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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WGA-D

Plasmid

#17989

Depositor

Natasha Raikhel

Article

Smith et al Plant Mol Biol. 1989 Nov . 13(5):601-3.

Insert

wheat germ agglutinin isolectin D

Expression

Bacterial

Mutation

N/A

Available Since

July 10, 2008

Availability

Academic Institutions and Nonprofits only
pMOS028: Mitycam calcium sensor (mitochondrial)

Plasmid

#163046

Purpose

Mitycam calcium sensor (mitochondrial)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

Mitycam

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pBIG1a

Plasmid

#80611

Purpose

pBIG1a - Step1 vector a (biGBac system for expression of protein complexes in insect cells)

Depositor

Jan-Michael Peters

Article

Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

Type

Empty backbone

Expression

Insect

Available Since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only

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