We narrowed to 5,900 results for: pcas

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AAV1 for Split-PE, SpCas9 nickase (PKC1001)

Plasmid

#190111

Purpose

AAV1 for expression of nickase SpCas9(H840A) for Split-PE use

Depositor

Keith Joung

Article

Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

Insert

nSpCas9

Use

AAV

Tags

bpNLS

Mutation

H840A

Promoter

EF1a

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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AAV pCAG-FLEX-tdTomato-WPRE

Plasmid

#51503

Purpose

Can be used to generate AAV virus that will express tdTomato in the presence of Cre

Depositor

Hongkui Zeng

Article

Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

Insert

tdTomato

Use

AAV

Promoter

pCAG

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pUC CBA-SpCas9.EF1a-BFP.sgLMNA

Plasmid

#98971

Purpose

Cas9 Homologous Recombination Reporter. SpCas9 and a sgRNA targeting LMNA. TagBFP.

Depositor

Jan Karlseder

Article

Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

Insert

LMNA sgRNA and spCas9

Expression

Mammalian

Available Since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-SpCas9(D10A)-T2A-EGFP

Plasmid

#221230

Purpose

Express nCas9 nickase (D10) with GFP reporter

Depositor

Fei Chen

Article

Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

Insert

SpCas9(D10A)-T2A-EGFP

Expression

Mammalian

Mutation

D10A

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGGS SARS-CoV-2 Alpha Spike

Plasmid

#185691

Purpose

Expresses codon-optimized full length SARS-CoV-2 Alpha Spike

Depositor

Marceline Côté

Article

Benlarbi et al iScience. 2022 Nov 18;25(11):105316. doi

Insert

SARS-CoV-2 Alpha Spike (S )

Expression

Mammalian

Mutation

Contains the following mutations: Δ69-70, Δ145, N…

Promoter

chicken β-actin promoter, CMV enhancer

Available Since

June 8, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pCAG PSAM4 GlyR IRES EGFP

Plasmid

#119739

Purpose

Chemogenetic inhibitor expression plasmid

Depositor

Scott Sternson

Article

Magnus et al Science. 2019 Mar 14. pii: science.aav52

Insert

PSAM4 GlyR IRES eGFP (CHRNA7 Synthetic, Human)

Expression

Mammalian

Mutation

L131G, Q139L, Y217F

Promoter

CAG

Available Since

March 18, 2019

Availability

Academic Institutions and Nonprofits only

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AAV pCAG-FLEX-EGFP-WPRE

Plasmid

#51502

Purpose

Can be used to generate AAV virus that will express EGFP in the presence of Cre

Depositor

Hongkui Zeng

Article

Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

Has Service

AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

Insert

EGFP

Use

AAV

Promoter

CAG

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-pCALM1-EYFP-WPRE-bGHpA

Plasmid

#213971

Purpose

AAV expression of EYFP from pCALM1 promoter

Depositor

Zhonghua Lu

Article

Wang et al Cell Rep. 2023 Nov 28;42(11):113348. doi

Insert

pCALM1

Use

AAV

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-GPHN-FingR-mGold2s-CCR5TC

Plasmid

#231785

Purpose

Visualization of inhibitory post-synapse using GPHN-FingR-mGold2s

Depositor

Francois St-Pierre

Article

Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

Insert

GPHN-FingR-mGold2s-CCR5TC

Expression

Mammalian

Promoter

Actin promoter with CMV enhancer

Available Since

March 5, 2025

Availability

Academic Institutions and Nonprofits only

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AAV pCAG-FLEX-mScarlet-WPRE

Plasmid

#99280

Purpose

Can be used to generate AAV virus that will express mScarlet in the presence of Cre

Depositor

Rylan Larsen

Insert

mScarlet

Use

AAV and Cre/Lox

Available Since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-SpCas9-P2A-EGFP (RTW3027)

Plasmid

#139987

Purpose

CMV and T7 promoter expression plasmid for human codon optimized SpCas9 with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Walton et al Science. 2020 Apr 17;368(6488):290-296.

Insert

human codon optimized SpCas9 with BPNLS-3xFLAG-P2A-EGFP

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS-3xFLAG-P2A-EGFP

Expression

Mammalian

Promoter

CMV and T7

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-MCP-dNS-SRRM1-V5-mCherry

Plasmid

#235095

Purpose

SRRM1 lacking Nuclear Speckle domain (dNS-SRRM1) mCherry fusion protein with MCP domain

Depositor

Mitchell Guttman

Article

Bhat et al Nature. 2024 May;629(8014):1165-1173. do

Insert

pCAG-MCP-dNS-SRRM1-V5-mCherry (SRRM1 Human)

Expression

Mammalian

Mutation

The SRRM1 entry clone from DNASU was modified usi…

Promoter

CAG

Available Since

May 7, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGG>nls-hCas9-nls-GFP

Plasmid

#99141

Purpose

Cas9-GFP fusion protein under the regulation of chicken beta-actin promoter

Depositor

Marianne Bronner

Article

Gandhi et al Dev Biol. 2017 Dec 1;432(1):86-97. doi:

Insert

Cas9-GFP

Use

CRISPR

Tags

GFP

Promoter

Chicken beta actin (CAG)

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-FLAG-puro-PTPIP51(1-470)

Plasmid

#170536

Purpose

Expresses PTPIP51(1-470) in human HeLa cells after transfection

Depositor

Byung Il Lee

Article

Yeo et al EMBO Rep. 2021 Jun 4;22(6):e51323. doi:

Insert

Protein tyrosine phosphatase interacting protein 51 (RMDN3 Human)

Tags

FLAG tag

Expression

Mammalian

Promoter

CAG promoter

Available Since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only
pCAGGS-SMS2-TEV-Twin-Strep

Plasmid

#202529

Purpose

Express C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged human Sphingomyelin synthase 2 (SMS2) in mammalian cell

Depositor

Chiaki Murakami

Article

Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

Insert

SGMS2 (SGMS2 Human)

Tags

TEV cleavable site and Twin-Strep-tag

Expression

Mammalian

Mutation

deleted stop codon (TGA)

Promoter

CAG and chicken β-actin promoter

Available Since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG PSAM4 5HT3 LC IRES GFP

Plasmid

#123066

Purpose

Chemogenetic activator expression plasmid

Depositor

Scott Sternson

Article

Magnus et al Science. 2019 Mar 14. pii: science.aav52

Insert

PSAM4 5HT3 LC IRES GFP (Htr3a Mouse)

Expression

Mammalian

Mutation

L131G Q139L Y217F

Promoter

CAG

Available Since

April 8, 2019

Availability

Academic Institutions and Nonprofits only

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pAG001 His6-TwinStrep-SUMO-AapCas12b

Plasmid

#153162

Purpose

Bacterial expression plasmid for AapCas12b

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Joung et al medRxiv. 2020 May 8. doi: 10.1101/2020.0

Insert

AapCas12b

Expression

Bacterial

Available Since

May 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE-NLS-dPspCas13b-3xmRuby3-NLS

Plasmid

#132405

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dPspCas13b

Use

Lentiviral

Expression

Mammalian

Mutation

H133A; H1059A

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pC0043-PspCas13b-gRNA non target

Plasmid

#223698

Purpose

gRNA control for dPspCas13b-FTO

Depositor

Chuan He

Article

Wei et al Science. 2022 May 27;376(6596):968-973.

Insert

gRNA target sequence for luciferase control

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pT3TS-spCas9 ABE-Umax-ex1

Plasmid

#222141

Purpose

Adenine base editor with modified TadA variant for extended window for A-to-G base editing in zebrafish

Depositor

Gaurav Varshney

Article

Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

Insert

Modified TadA-nSpCas9

Use

CRISPR; Zebrafish expression

Tags

Bipartite NLS

Promoter

T3

Available Since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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