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SpG_ATH51-IP

Plasmid

#242074

Purpose

Expresses Cas9-SpG_ATH51 in mammalian cells

Depositor

Tobias Cantz

Article

Eggenschwiler et al Nucleic Acids Res. 2025 Aug 11;53(15):gk

Insert

SpG_ATH51

Use

CRISPR

Expression

Mammalian

Mutation

ectopic insertion of 7 amino acids of PID turn-he…

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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Aga2p-TEVcs-LacAnc100-myc_pCTCON2

Plasmid

#245298

Purpose

expresses LaccID on the yeast surface

Depositor

Alice Ting

Article

Lee et al Nat Chem Biol. 2025 Aug 1. doi: 10.1038/

Insert

Aga2p-LacAnc100

Expression

Yeast

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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TRE3G-ZIM3-Cas9-P2A-GFP

Plasmid

#239605

Purpose

Doxycycline inducible CRISPRgenee construct

Depositor

Philipp Rathert

Article

Stadager et al Cell Rep Methods. 2025 Jun 16;5(6):10107

Insert

ZIM3 KRAB domain (ZIM3 )

Use

CRISPR and Lentiviral

Promoter

TRE3G

Available Since

Sept. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV_SS-HA-V5-LacAnc100-CD4 TM_pDisplay

Plasmid

#245299

Purpose

expresses LaccID on the mammalian cell surface

Depositor

Alice Ting

Article

Lee et al Nat Chem Biol. 2025 Aug 1. doi: 10.1038/

Insert

LacAnc100-CD4TM

Expression

Yeast

Available Since

Sept. 30, 2025

Availability

Academic Institutions and Nonprofits only

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SpRY_SlTH51-IP

Plasmid

#235993

Purpose

Expresses SpRY_SlTH51 in mammalian cells

Depositor

Tobias Cantz

Article

Eggenschwiler et al Nucleic Acids Res. 2025 Aug 11;53(15):gk

Insert

SpRY_ATH51

Use

CRISPR

Expression

Mammalian

Mutation

ectopic insertion of 8 amino acids of PID turn-he…

Promoter

EF-1a

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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PB-PE iSM

Plasmid

#235995

Purpose

Expresses iSM (iSpyMac) prime editor and pegRNA in mammalian cells

Depositor

Tobias Cantz

Article

Eggenschwiler et al Nucleic Acids Res. 2025 Aug 11;53(15):gk

Insert

iSM_H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)

Use

CRISPR and Synthetic Biology; Piggybac transposon

Tags

bpSV40 NLS

Expression

Mammalian

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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iSM_PPL2-IP

Plasmid

#235989

Purpose

Expresses iSM_PPL2 in mammalian cells

Depositor

Tobias Cantz

Article

Eggenschwiler et al Nucleic Acids Res. 2025 Aug 11;53(15):gk

Insert

iSM_PPL2

Use

CRISPR

Expression

Mammalian

Mutation

replacement of 5 amino acids of Smac-Cas9 PL2 wit…

Promoter

EF-1a

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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SpRY_MTH51-IP

Plasmid

#235992

Purpose

Expresses SpRY_MTH51 in mammalian cells

Depositor

Tobias Cantz

Article

Eggenschwiler et al Nucleic Acids Res. 2025 Aug 11;53(15):gk

Insert

SpRY_MTH51

Use

CRISPR

Expression

Mammalian

Mutation

ectopic insertion of 7 amino acids of PID turn-he…

Promoter

EF-1a

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pMT-Myc-BirA*G3

Plasmid

#240233

Purpose

Expression of Myc-BirA*-G3 under control of copper-inducible promoter. Can be used to generate stable cell lines by Hygromycin selection.

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al bioRxiv [Preprint]. 2025 Jul 11:2025.07.

Insert

Myc-BirA*G3

Expression

Insect

Promoter

MT

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pJRoC30-LacAnc100

Plasmid

#234653

Purpose

Expresses LacAnc100 variant in yeast

Depositor

Miguel Alcalde Galeote, Alice Ting

Article

Lee et al Nat Chem Biol. 2025 Aug 1. doi: 10.1038/

Insert

LacAnc100

Tags

HRV 3C protease site-GSG linker-8xHis tag

Expression

Yeast

Promoter

Gal1p

Available Since

Aug. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR_CG6867_nostop

Plasmid

#240238

Purpose

Gateway entry clone with CG6867 without stop codon

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al bioRxiv [Preprint]. 2025 Jul 11:2025.07.

Insert

CG6867 (CG6867 Fly)

Use

Gateway shuttle vector

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pWal10-roe-sfGFP

Plasmid

#240239

Purpose

Gateway destination plasmid to generate C-terminal sfGFP fusions under control of UAS promoter. Can be used to generate transgenic fly lines by white+ fly eye selection and phiC31 integration.

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al bioRxiv [Preprint]. 2025 Jul 11:2025.07.

Type

Empty backbone

Use

Gateway destination vector

Expression

Insect

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pBS-GMR-eya(shRNA)_EcoRImut

Plasmid

#240222

Purpose

pBS-GMR-eya(shRNA) vector with one of two EcoRI sites mutated (T->A)

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al bioRxiv [Preprint]. 2025 Jul 11:2025.07.

Type

Empty backbone

Expression

Insect

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HaloAKAR2.0-T2A-EGFP

Plasmid

#216488

Purpose

Expression of chemigenetic PKA biosensor (high basal brightness, medium dynamic range) and EGFP transfection marker in mammalian cells

Depositor

Jin Zhang

Article

Frei et al Nat Biotechnol. 2025 Apr 21. doi: 10.103

Insert

HaloAKAR2.0

Tags

T2A-EGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 20, 2025

Availability

Academic Institutions and Nonprofits only

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BtARR1 Cys-less

Plasmid

#236274

Purpose

Bacterial expression of bovine arrestin-1 C63A, C128S, C143A, inserted GGT codon after starting ATG

Depositor

Vsevolod Gurevich

Article

Hanson et al Proc Natl Acad Sci U S A. 2006 Mar 28;10

Insert

arrestin-1 (SAG Bovine)

Expression

Bacterial

Mutation

C63A, C128S, C143A, inserted GGT codon after star…

Promoter

lac

Available Since

May 15, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNAintron-CHIKV-Structural polyprotein with Nluc tagged E2

Plasmid

#215699

Purpose

Produces the Chikungunya virus structural polyprotein Capsid-E3-NLucE2-6K-E1

Depositor

Melinda Brindley

Article

Reyes Ballista et al J Virol. 2024 Jul 15:e0077524. doi: 10.1

Insert

CHIKV structural polyprotein Capsid-E3-NLucE2-6K-E1

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 1, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-EF1a-ArgiNLS-miRFP670

Plasmid

#220600

Purpose

Constitutive expression of a single-cell discriminating version of miRFP670 fluorescent protein.

Depositor

Sam Golden

Article

Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

Insert

miRFP670

Use

AAV

Tags

ArgiNLS

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-ArgiNLS-mVenus-Q69M(ME)

Plasmid

#220597

Purpose

Constitutive expression of a single-cell discriminating version of mVenus-Q69M (ME) fluorescent protein.

Depositor

Sam Golden

Article

Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

Insert

mVenus-Q69M (ME)

Use

AAV

Tags

ArgiNLS

Expression

Mammalian

Promoter

EF1a

Available Since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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pLC045 - pL0_CrGLK (CDS1ns)

Plasmid

#203904

Purpose

MoClo compatible L0 plasmid containing the CrGLK coding sequence, lacking a stop codon to allow for C-terminal fusions.

Depositor

Carolyn Lee-Parsons

Article

Cole-Osborn et al Plant Cell Rep. 2024 May 14;43(6):141. d

Insert

CrGLK (without a stop codon)

Expression

Plant

Mutation

Silent mutation of BpiI or BsaI cut site for MoCl…

Available Since

April 26, 2024

Availability

Academic Institutions and Nonprofits only

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pLC047 - pL2_p2x35s-CrGLK-tMAS

Plasmid

#203906

Purpose

Overexpression of the CrGLK coding sequence from C. roseus in a plant binary vector for Agrobacterium-mediated transformation.

Depositor

Carolyn Lee-Parsons

Article

Cole-Osborn et al Plant Cell Rep. 2024 May 14;43(6):141. d

Insert

CrGLK

Expression

Plant

Mutation

Silent mutation of BpiI or BsaI cut site for MoCl…

Promoter

CaMV 2x35S

Available Since

April 26, 2024

Availability

Academic Institutions and Nonprofits only

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