We narrowed to 2,612 results for: ubiquitin

1. p663-UBC-miniTurbo-V5-CDK7_IDG-K

   Plasmid

   #158194

   Purpose

   Gateway destination clone of CDK7 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   miniTurbo-V5-CDK7 (CDK7 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   miniTurbo-V5

   Expression

   Mammalian

   Mutation

   Promoter

   Ubiquitin

   Available since

   Jan. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV-SFFV-RAB10-WPRE-UbC-Emerald

   Plasmid

   #203804

   Purpose

   Lentiviral vector plasmid expressing human RAB10 under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

   Depositor

   Bart Nieuwenhuis, Richard Eva

   Inserts

   RAB10 (RAB10 Human)
   Emerald
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. p663-UBC-miniTurbo-V5-STK3_IDG-K

   Plasmid

   #189879

   Purpose

   Gateway destination clone of STK3 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   STK3 (STK3 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   miniTurbo-V5

   Expression

   Mammalian

   Mutation

   Promoter

   Ubiquitin

   Available since

   Oct. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. p663-UBC-miniTurbo-V5-MARK3_IDG-K

   Plasmid

   #170583

   Purpose

   Gateway destination clone of MARK3 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   miniTurbo-V5-MARK3 (MARK3 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   miniTurbo-V5

   Expression

   Mammalian

   Mutation

   Promoter

   Ubiquitin

   Available since

   Sept. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. p667-UBC-MAP3K14-V5-miniTurbo_IDG-K

   Plasmid

   #189903

   Purpose

   Gateway destination clone of MAP3K14 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   MAP3K14 (MAP3K14 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   V5-miniTurbo

   Expression

   Mammalian

   Mutation

   Promoter

   Ubiquitin

   Available since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-Dub3 mouse

   Plasmid

   #72682

   Purpose

   mammalian expression of mouse Dub3

   Depositor

   Domenico Maiorano

   Article

   van_der_Laan et al Mol Cell. 2013 Nov 7;52(3):366-79. doi:

   Insert

   Dub3 (Usp17le Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

7. p663-UBC-miniTurbo-V5-DSTYK _IDG-K

   Plasmid

   #158182

   Purpose

   Gateway destination clone of DSTYK (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   miniTurbo-V5-DSTYK (DSTYK Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   miniTurbo-V5

   Expression

   Mammalian

   Mutation

   Promoter

   Ubiquitin

   Available since

   Oct. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pZH051

   Plasmid

   #47311

   Purpose

   Expresses Tsr-Venus-Ubiquitin-CI

   Depositor

   Jie Xiao

   Article

   Hensel et al Nat Struct Mol Biol. 2012 Aug;19(8):797-

   Insert

   wild type immunity region of lambda phage with tsr-venus-ub-cI
   

   Use

   Tags

   CI

   Expression

   Bacterial

   Mutation

   Promoter

   PR; PRM

   Available since

   Sept. 12, 2013

   Availability

   Academic Institutions and Nonprofits only

9. p667-UBC-DYRK3-V5-miniTurbo_IDG-K

   Plasmid

   #158190

   Purpose

   Gateway destination clone of DYRK3 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   DYRK3-V5-miniTurbo (DYRK3 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   V5-miniTurbo

   Expression

   Mammalian

   Mutation

   R293H- please see depositor comments

   Promoter

   Ubiquitin

   Available since

   April 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. p663-UBC-miniTurbo-V5-CAMK1D_IDG-K

    Plasmid

    #158167

    Purpose

    Gateway destination clone of CAMK1D (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    miniTurbo-V5-CAMK1D (CAMK1D Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. Pub-mCD8:GFP-T2A-DsRed:NLS-SV40

    Plasmid

    #130665

    Purpose

    Plasmid to test the efficiency of T2A multiplexing in Aedes aegypti

    Depositor

    Leslie Vosshall

    Article

    Matthews et al eLife. 2019 May 21;8. pii: 43963. doi: 1

    Insert

    AaegPUb-mCD8-GFP-T2A-dsRed-NLS-SV40
    

    Use

    Ae. aegypti expression

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Aedes aegypti poly-ubiquitin

    Available since

    Oct. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKF251

    Plasmid

    #52532

    Purpose

    Double-GFP reporter for DNA cleavage

    Depositor

    Klaus Foerstemann

    Article

    Bottcher et al Nucleic Acids Res. 2014 Apr 19.

    Insert

    Double-GFP
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    ubiquitin

    Available since

    April 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. Ube2i

    Plasmid

    #31420

    Depositor

    Rachel Klevit

    Article

    Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):9

    Insert

    Ubiquitin Conjugating Enzyme (UBE2I Human)
    

    Use

    Tags

    HA

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

14. pLANT-2b

    Plasmid

    #231487

    Purpose

    Expresses His6-SUMO^NIT-FraB where "NIT" stands for "no internal translation", reflecting the mutation of the two Shine-Dalgarno-like sequences within the SUMO tag.

    Depositor

    Venkat Gopalan

    Inserts

    SUMO^NIT (SMT3 Budding Yeast)
    FraB
    

    Use

    Tags

    His6 tag and His6-SUMO^NIT

    Expression

    Bacterial

    Mutation

    Deleted amino acids 1-6 (MEPEES). and M83L, E84Q,…

    Promoter

    T7 promoter

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. E547* FL USP7

    Plasmid

    #229001

    Purpose

    E547-mutant of human USP7

    Depositor

    Irina Bezsonova

    Insert

    USP7 1-546 (USP7 Human)
    

    Use

    Tags

    6xHis cleavable with thrombin

    Expression

    Bacterial

    Mutation

    E547 mutated to stop codon

    Promoter

    T7

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLVUT-spGFP

    Plasmid

    #225486

    Purpose

    Tet-on lentiviral vector for controlled expression of secretable and permeable EGFP

    Depositor

    Robert Gross

    Article

    Gutekunst et al Neuroscience. 2016 Dec 17;339:308-318. d

    Insert

    Igκ leader and TAT peptide
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Human ubiquitin C

    Available since

    Sept. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLV-SFFV-ZFYVE27 S14D S25D S32D T261D-WPRE-UbC-Emerald

    Plasmid

    #214468

    Purpose

    Lentiviral vector plasmid expressing human ZFYVE27 mutations S14D S25D S32D T261D under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

    Depositor

    Bart Nieuwenhuis, Richard Eva

    Inserts

    ZFYVE27 (ZFYVE27 Human)
    Emerald
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    S14D S25D S32D T261D

    Promoter

    Available since

    July 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLV-SFFV-ZFYVE27 S14D S25D S32D T261D-WPRE-UbC-mCherry

    Plasmid

    #214471

    Purpose

    Lentiviral vector plasmid expressing human ZFYVE27 mutations S14D S25D S32D T261D under the spleen focus-forming virus (SFFV) promoter and mCherry under the Ubiquitin C (UbC) promoter

    Depositor

    Bart Nieuwenhuis, Richard Eva

    Inserts

    ZFYVE27 (ZFYVE27 Human)
    mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    S14D S25D S32D T261D

    Promoter

    Available since

    March 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. FB408

    Plasmid

    #203629

    Purpose

    Promoter of the ubiquitin ligase gene from P. digitatum (PDIG_07760).

    Depositor

    Jose Marcos

    Article

    Moreno-Gimenez et al Front Bioeng Biotechnol. 2023 Aug 7;11:1

    Insert

    P07760
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLV-SFFV-Llgl1-WPRE-UbC-Emerald

    Plasmid

    #203805

    Purpose

    Lentiviral vector plasmid expressing mouse Llgl1 under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

    Depositor

    Bart Nieuwenhuis, Richard Eva

    Inserts

    Llgl1 (Llgl1 Mouse)
    Emerald
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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