We narrowed to 2,618 results for: ubiquitin

1. p663-UBC-miniTurbo-V5-AKT1_IDG-K

   Plasmid

   #189890

   Purpose

   Gateway destination clone of AKT1 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   AKT1 (AKT1 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   miniTurbo-V5

   Expression

   Mammalian

   Mutation

   Promoter

   Ubiquitin

   Available since

   Oct. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGAPi

   Plasmid

   #127547

   Purpose

   Gateway-based RNAi vector to clone target gene for silencing in tandem with APT transcript segment

   Depositor

   Luis Vidali

   Article

   Orr et al Plant Physiol. 2020 Aug 6. pii: pp.20.00

   Type

   Empty backbone

   Use

   RNAi

   Tags

   Expression

   Mutation

   Promoter

   Ubiquitin

   Available since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. p663-UBC-miniTurbo-V5-CDK19_IDG-K

   Plasmid

   #158175

   Purpose

   Gateway destination clone of CDK19 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   miniTurbo-V5-CDK19 (CDK19 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   miniTurbo-V5

   Expression

   Mammalian

   Mutation

   Promoter

   Ubiquitin

   Available since

   Sept. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCellFree_G03 WWP1

   Plasmid

   #67125

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   WWP1 (WWP1 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Expression

   Mutation

   Promoter

   T7

   Available since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. p663-UBC-miniTurbo-V5-ERN2_IDG-K

   Plasmid

   #189905

   Purpose

   Gateway destination clone of ERN2 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   ERN2 (ERN2 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   miniTurbo-V5

   Expression

   Mammalian

   Mutation

   Promoter

   Ubiquitin

   Available since

   Dec. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. p663-UBC-miniTurbo-V5-CDK7_IDG-K

   Plasmid

   #158194

   Purpose

   Gateway destination clone of CDK7 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   miniTurbo-V5-CDK7 (CDK7 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   miniTurbo-V5

   Expression

   Mammalian

   Mutation

   Promoter

   Ubiquitin

   Available since

   Jan. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLV-SFFV-RAB10-WPRE-UbC-Emerald

   Plasmid

   #203804

   Purpose

   Lentiviral vector plasmid expressing human RAB10 under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

   Depositor

   Bart Nieuwenhuis, Richard Eva

   Inserts

   RAB10 (RAB10 Human)
   Emerald
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. p663-UBC-miniTurbo-V5-STK3_IDG-K

   Plasmid

   #189879

   Purpose

   Gateway destination clone of STK3 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   STK3 (STK3 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   miniTurbo-V5

   Expression

   Mammalian

   Mutation

   Promoter

   Ubiquitin

   Available since

   Oct. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. p663-UBC-miniTurbo-V5-MARK3_IDG-K

   Plasmid

   #170583

   Purpose

   Gateway destination clone of MARK3 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   miniTurbo-V5-MARK3 (MARK3 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   miniTurbo-V5

   Expression

   Mammalian

   Mutation

   Promoter

   Ubiquitin

   Available since

   Sept. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. p667-UBC-MAP3K14-V5-miniTurbo_IDG-K

    Plasmid

    #189903

    Purpose

    Gateway destination clone of MAP3K14 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    MAP3K14 (MAP3K14 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5-miniTurbo

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pZH051

    Plasmid

    #47311

    Purpose

    Expresses Tsr-Venus-Ubiquitin-CI

    Depositor

    Jie Xiao

    Article

    Hensel et al Nat Struct Mol Biol. 2012 Aug;19(8):797-

    Insert

    wild type immunity region of lambda phage with tsr-venus-ub-cI
    

    Use

    Tags

    CI

    Expression

    Bacterial

    Mutation

    Promoter

    PR; PRM

    Available since

    Sept. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

12. p663-UBC-miniTurbo-V5-DSTYK _IDG-K

    Plasmid

    #158182

    Purpose

    Gateway destination clone of DSTYK (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    miniTurbo-V5-DSTYK (DSTYK Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    Oct. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. p667-UBC-DYRK3-V5-miniTurbo_IDG-K

    Plasmid

    #158190

    Purpose

    Gateway destination clone of DYRK3 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    DYRK3-V5-miniTurbo (DYRK3 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5-miniTurbo

    Expression

    Mammalian

    Mutation

    R293H- please see depositor comments

    Promoter

    Ubiquitin

    Available since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. Pub-mCD8:GFP-T2A-DsRed:NLS-SV40

    Plasmid

    #130665

    Purpose

    Plasmid to test the efficiency of T2A multiplexing in Aedes aegypti

    Depositor

    Leslie Vosshall

    Article

    Matthews et al eLife. 2019 May 21;8. pii: 43963. doi: 1

    Insert

    AaegPUb-mCD8-GFP-T2A-dsRed-NLS-SV40
    

    Use

    Ae. aegypti expression

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Aedes aegypti poly-ubiquitin

    Available since

    Oct. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKF251

    Plasmid

    #52532

    Purpose

    Double-GFP reporter for DNA cleavage

    Depositor

    Klaus Foerstemann

    Article

    Bottcher et al Nucleic Acids Res. 2014 Apr 19.

    Insert

    Double-GFP
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    ubiquitin

    Available since

    April 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. Ube2i

    Plasmid

    #31420

    Depositor

    Rachel Klevit

    Article

    Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):9

    Insert

    Ubiquitin Conjugating Enzyme (UBE2I Human)
    

    Use

    Tags

    HA

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

17. pLANT-2b

    Plasmid

    #231487

    Purpose

    Expresses His6-SUMO^NIT-FraB where "NIT" stands for "no internal translation", reflecting the mutation of the two Shine-Dalgarno-like sequences within the SUMO tag.

    Depositor

    Venkat Gopalan

    Inserts

    SUMO^NIT (SMT3 Budding Yeast)
    FraB
    

    Use

    Tags

    His6 tag and His6-SUMO^NIT

    Expression

    Bacterial

    Mutation

    Deleted amino acids 1-6 (MEPEES). and M83L, E84Q,…

    Promoter

    T7 promoter

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. E547* FL USP7

    Plasmid

    #229001

    Purpose

    E547-mutant of human USP7

    Depositor

    Irina Bezsonova

    Insert

    USP7 1-546 (USP7 Human)
    

    Use

    Tags

    6xHis cleavable with thrombin

    Expression

    Bacterial

    Mutation

    E547 mutated to stop codon

    Promoter

    T7

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLVUT-spGFP

    Plasmid

    #225486

    Purpose

    Tet-on lentiviral vector for controlled expression of secretable and permeable EGFP

    Depositor

    Robert Gross

    Article

    Gutekunst et al Neuroscience. 2016 Dec 17;339:308-318. d

    Insert

    Igκ leader and TAT peptide
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Human ubiquitin C

    Available since

    Sept. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLV-SFFV-ZFYVE27 S14D S25D S32D T261D-WPRE-UbC-Emerald

    Plasmid

    #214468

    Purpose

    Lentiviral vector plasmid expressing human ZFYVE27 mutations S14D S25D S32D T261D under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

    Depositor

    Bart Nieuwenhuis, Richard Eva

    Inserts

    ZFYVE27 (ZFYVE27 Human)
    Emerald
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    S14D S25D S32D T261D

    Promoter

    Available since

    July 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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