We narrowed to 32,525 results for: Eng

1. pYPQ265N1

   Plasmid

   #173998

   Purpose

   Gateway entry clone (attL1 & attR5) rAPOBEC1-zCas9(D10A)-UNG for C-G base editing

   Depositor

   Yiping Qi

   Article

   Sretenovic et al Front Genome Ed. 2021 Sep 15;3:756766. d

   Insert

   rAPOBEC1-zCas9(D10A)-UNG
   

   Use

   CRISPR

   Tags

   3X FLAG, NLS

   Expression

   Plant

   Mutation

   D10A

   Available Since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pYPQ265O1

   Plasmid

   #173999

   Purpose

   Gateway entry clone (attL1 & attR5) rAPOBEC1-zCas9(D10A)-rXRCC1 for C-G base editing

   Depositor

   Yiping Qi

   Article

   Sretenovic et al Front Genome Ed. 2021 Sep 15;3:756766. d

   Insert

   rAPOBEC1-zCas9(D10A)-rXRCC1
   

   Use

   CRISPR

   Tags

   3X FLAG, NLS

   Expression

   Plant

   Mutation

   D10A

   Available Since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pJAID6JY

   Plasmid

   #165049

   Purpose

   Integrative expression of TRX1-AID* (mini auxin inducible degron)-yEGFP and rice auxin receptor gene OsTIR1.

   Depositor

   Claudia Vickers

   Article

   Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

   Insert

   P-URA3>KlURA3>T-AgTEF1-P-TEF1>TRX1-AID*>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3
   

   Expression

   Yeast

   Mutation

   WT

   Available Since

   March 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pJAID8Z105

   Plasmid

   #165057

   Purpose

   Integrative expression of nucleus-targeting yEGFP-AID*-CUP1 and SKP1-fusing rice auxin receptor gene OsTIR1.

   Depositor

   Claudia Vickers

   Article

   Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

   Insert

   P-URA3>KlURA3>T-AgTEF1-P-TEF1>yEGFP>AID*>CUP1>SV40NSL>T-PGK1-P-ACS2>SKP1>OsTIR1opt>T-URA3
   

   Expression

   Yeast

   Mutation

   WT

   Available Since

   March 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pJAID9L105

   Plasmid

   #165055

   Purpose

   Integrative expression of nucleus-targeting yEGFP-AID*-CUP1 and rice auxin receptor gene OsTIR1.

   Depositor

   Claudia Vickers

   Article

   Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

   Insert

   P-URA3>KlURA3>T-AgTEF1-P-TEF1>yEGFP>AID*>CUP1>SV40NSL>T-PGK1-P-ACS2>OsTIR1opt>T-URA3
   

   Expression

   Yeast

   Mutation

   WT

   Available Since

   March 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pJAID9L

   Plasmid

   #165052

   Purpose

   Integrative expression of yEGFP and rice auxin receptor gene OsTIR1. OsTIR1 is codon-optimised for expression in S. cerevisiae

   Depositor

   Claudia Vickers

   Article

   Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

   Insert

   P-URA3>KlURA3>T-AgTEF1-P-TEF1>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3
   

   Expression

   Yeast

   Mutation

   WT

   Available Since

   March 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pJAID5Z

   Plasmid

   #165047

   Purpose

   Integrative expression of AID* (mini auxin inducible degron)-yEGFP and rice auxin receptor gene OsTIR1.

   Depositor

   Claudia Vickers

   Article

   Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

   Insert

   P-URA3>KlURA3>T-AgTEF1-P-TEF1>AID*>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3
   

   Expression

   Yeast

   Mutation

   OsTIR1 is codon-optimised for expression in S. ce…

   Available Since

   March 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pYPQ141-ZmUbi-RZ-Bth

   Plasmid

   #129686

   Purpose

   (Empty backbone) Gateway entry clone with sgRNA under ZmUbi promoter

   Depositor

   Yiping Qi

   Article

   Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

   Insert

   Empty Backbone
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   March 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pWDY1226

   Plasmid

   #138593

   Purpose

   Expresses NatL2-R156A in E.coli

   Depositor

   James Naismith

   Article

   Song et al Angew Chem Int Ed Engl. 2020 Jan 5. doi:

   Insert

   phenylacetate-coenzyme A ligase
   

   Tags

   6-His

   Expression

   Bacterial

   Mutation

   changed Arginine 156 to Alanine

   Promoter

   T7

   Available Since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pWDY1222

    Plasmid

    #138592

    Purpose

    Expresses NatL2-K418A in E.coli

    Depositor

    James Naismith

    Article

    Song et al Angew Chem Int Ed Engl. 2020 Jan 5. doi:

    Insert

    phenylacetate-coenzyme A ligase
    

    Tags

    6-His

    Expression

    Bacterial

    Mutation

    changed Lysine 418 to Alanine

    Promoter

    T7

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pYPQ141-ZmUbi-RZ-Bh

    Plasmid

    #136378

    Purpose

    Gateway entry clone for BhCas12b sgRNA expression under ZmUbi promoter with ribozyme processing

    Depositor

    Yiping Qi

    Article

    Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Feb. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCAG-Ace1_Q89D_D100N_E206V-HaloTag

    Plasmid

    #129255

    Purpose

    Express Ace1_Q89D_D100N_E206V-HaloTag in mammalian cells

    Depositor

    Eric Schreiter

    Article

    Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

    Insert

    Ace1_Q89D_D100N_E206V-HaloTag
    

    Expression

    Mammalian

    Mutation

    Q89D, D100N, E206V

    Promoter

    CAG

    Available Since

    Aug. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCAG-Mac_Q139D_D150N_E258V-HaloTag-ST

    Plasmid

    #129264

    Purpose

    Express soma-localized Mac_Q139D_D150N_E258V-HaloTag in mammalian cells

    Depositor

    Eric Schreiter

    Article

    Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

    Insert

    Mac_Q139D_D150N_E258V-HaloTag-ST
    

    Expression

    Mammalian

    Mutation

    Q139D,D150N,E258V

    Promoter

    CAG

    Available Since

    Aug. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCAG-QuaAr3_Q95D_H106N_E214V-HaloTag-ST

    Plasmid

    #129263

    Purpose

    Express soma-localized QuaAr3_Q95D_H106N_E214V-HaloTag in mammalian cells

    Depositor

    Eric Schreiter

    Article

    Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

    Insert

    QuaAr3_Q95D_H106N_E214V-HaloTag-ST
    

    Expression

    Mammalian

    Mutation

    Q95D, H106N, E214V

    Promoter

    CAG

    Available Since

    Aug. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCAG-Ace1_Q89D_D100N_E206V-HaloTag-ST

    Plasmid

    #129260

    Purpose

    Express soma-localized Ace1_Q89D_D100N_E206V-HaloTag in mammalian cells

    Depositor

    Eric Schreiter

    Article

    Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

    Insert

    Ace1_Q89D_D100N_E206V-HaloTag-ST
    

    Expression

    Mammalian

    Mutation

    Q89D, D100N, E206V

    Promoter

    CAG

    Available Since

    Aug. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCAG-Mac_Q139D_D150N_E258V-HaloTag

    Plasmid

    #129259

    Purpose

    Express Mac_Q139D_D150N_E258V-HaloTag in mammalian cells

    Depositor

    Eric Schreiter

    Article

    Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

    Insert

    Mac_Q139D_D150N_E258V-HaloTag
    

    Expression

    Mammalian

    Mutation

    Q139D,D150N,E258V

    Promoter

    CAG

    Available Since

    Aug. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCAG-QuaAr3_Q95D_H106N_E214V-HaloTag

    Plasmid

    #129258

    Purpose

    Express QuaAr3_Q95D_H106N_E214V-HaloTag in mammalian cells

    Depositor

    Eric Schreiter

    Article

    Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

    Insert

    QuaAr3_Q95D_H106N_E214V-HaloTag
    

    Expression

    Mammalian

    Mutation

    Q95D, H106N, E214V

    Promoter

    CAG

    Available Since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET51b(+)_MBP-nLucCP-65/68

    Plasmid

    #113921

    Purpose

    Plasmid encoding the bacterial expression of the MBP fused NanoLuc circular permuted between the position 65 and 68

    Depositor

    Kai Johnsson

    Article

    Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

    Insert

    MBP-NanoLucCP-65/68
    

    Use

    Luciferase

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET51b(+)_MBP-nLucCP-65/66

    Plasmid

    #113920

    Purpose

    Plasmid encoding the bacterial expression of the MBP fused NanoLuc circular permuted between the position 65 and 66

    Depositor

    Kai Johnsson

    Article

    Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

    Insert

    MBP-NanoLucCP-65/66
    

    Use

    Luciferase

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pT7T3D-PacI-Tmeff2

    Plasmid

    #99728

    Purpose

    to make in situ RNA probes for Tmeff2

    Depositor

    Mengsheng Qiu

    Article

    Huang et al Sci Rep. 2017 Mar 23;7(1):337. doi: 10.1

    Insert

    Tmeff2 (Tmeff2 Mouse)
    

    Use

    In situ probe

    Available Since

    Feb. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge