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pCHC023 (∆MBH)

Plasmid

#226201

Purpose

Electroporation knockout plasmid (pK18sB) to delete the C. necator membrane-bound hydrogenase operon (deltaMBH).

Depositor

Christopher Johnson

Article

Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

Insert

Homology arms for membrane bound hydrogenase operon

Use

Synthetic Biology

Mutation

∆hoxKGZMLOQRTV ∆hypA1B1F1CDEX ∆hoxABCJ

Available Since

Nov. 18, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pACB108

Plasmid

#222207

Purpose

For recombinant expression of fghA (PP_1617 from Pseudomonas putida) with a C-terminal His tag

Depositor

Gregg Beckham

Article

Bleem et al Metab Eng. 2024 Jul;84:145-157. doi: 10.

Insert

fghA

Tags

6x His

Expression

Bacterial

Promoter

T7

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pACB109

Plasmid

#222208

Purpose

For recombinant expression of fghA (PP_1617 from Pseudomonas putida) with the K10N mutation and a C-terminal His tag

Depositor

Gregg Beckham

Article

Bleem et al Metab Eng. 2024 Jul;84:145-157. doi: 10.

Insert

fghA (K10N)

Tags

6x His

Expression

Bacterial

Mutation

Changed Lys10 to Asn

Promoter

T7

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pACB110

Plasmid

#222209

Purpose

For recombinant expression of fghA (PP_1617 from Pseudomonas putida) with the G258D mutation and a C-terminal His tag

Depositor

Gregg Beckham

Article

Bleem et al Metab Eng. 2024 Jul;84:145-157. doi: 10.

Insert

fghA (G258D)

Tags

6x His

Expression

Bacterial

Mutation

Changed Gly258 to Aspartate

Promoter

T7

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pACB108

Plasmid

#222207

Purpose

For recombinant expression of fghA (PP_1617 from Pseudomonas putida) with a C-terminal His tag

Depositor

Gregg Beckham

Article

Bleem et al Metab Eng. 2024 Jul;84:145-157. doi: 10.

Insert

fghA

Tags

6x His

Expression

Bacterial

Promoter

T7

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pACB109

Plasmid

#222208

Purpose

For recombinant expression of fghA (PP_1617 from Pseudomonas putida) with the K10N mutation and a C-terminal His tag

Depositor

Gregg Beckham

Article

Bleem et al Metab Eng. 2024 Jul;84:145-157. doi: 10.

Insert

fghA (K10N)

Tags

6x His

Expression

Bacterial

Mutation

Changed Lys10 to Asn

Promoter

T7

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pACB110

Plasmid

#222209

Purpose

For recombinant expression of fghA (PP_1617 from Pseudomonas putida) with the G258D mutation and a C-terminal His tag

Depositor

Gregg Beckham

Article

Bleem et al Metab Eng. 2024 Jul;84:145-157. doi: 10.

Insert

fghA (G258D)

Tags

6x His

Expression

Bacterial

Mutation

Changed Gly258 to Aspartate

Promoter

T7

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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(R)pGPD1(D)_P1(SBE109)

Plasmid

#194993

Purpose

promoter of the gene glyceraldehyde-3-phosphate dehydrogenase from R. toruloides for a P1 position (overhangs R/D)

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

(R) pGPD (D)

Expression

Bacterial

Available Since

Feb. 26, 2024

Availability

Academic Institutions and Nonprofits only

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(R)pGPD1(D)_P1(SBE109)

Plasmid

#194993

Purpose

promoter of the gene glyceraldehyde-3-phosphate dehydrogenase from R. toruloides for a P1 position (overhangs R/D)

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

(R) pGPD (D)

Expression

Bacterial

Available Since

Feb. 26, 2024

Availability

Academic Institutions and Nonprofits only

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WT_IFL_A28C_R

Plasmid

#200184

Purpose

WT PA14 I-F Leader and A28C Repeat

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

Wildtype I-F Leader and a mutant I-F Locus (A28C)

Expression

Bacterial

Mutation

A28C in the I-F repeat

Available Since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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WT_IFL_A28C_R

Plasmid

#200184

Purpose

WT PA14 I-F Leader and A28C Repeat

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

Wildtype I-F Leader and a mutant I-F Locus (A28C)

Expression

Bacterial

Mutation

A28C in the I-F repeat

Available Since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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(F)pGPD(G)_P2 (SBE110)

Plasmid

#194992

Purpose

promoter of the gene glyceraldehyde-3-phosphate dehydrogenase from R. toruloides for a P2 position (overhangs F/G)

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

(F) pGPD (G)

Expression

Bacterial

Available Since

Dec. 7, 2023

Availability

Academic Institutions and Nonprofits only

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(F)pGPD(G)_P2 (SBE110)

Plasmid

#194992

Purpose

promoter of the gene glyceraldehyde-3-phosphate dehydrogenase from R. toruloides for a P2 position (overhangs F/G)

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

(F) pGPD (G)

Expression

Bacterial

Available Since

Dec. 7, 2023

Availability

Academic Institutions and Nonprofits only

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(I) pADH2 (J) P3 (SBE114)

Plasmid

#187629

Purpose

promoter of the gene alcohol dehydrogenase 2 from R. toruloides for the P3 position (I/J)

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

(I) pADH2 (J)

Expression

Bacterial

Available Since

Dec. 7, 2023

Availability

Academic Institutions and Nonprofits only

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(I) pADH2 (J) P3 (SBE114)

Plasmid

#187629

Purpose

promoter of the gene alcohol dehydrogenase 2 from R. toruloides for the P3 position (I/J)

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

(I) pADH2 (J)

Expression

Bacterial

Available Since

Dec. 7, 2023

Availability

Academic Institutions and Nonprofits only

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(F) pADH2 (G)_P2 (SBE113)

Plasmid

#187628

Purpose

promoter of the gene alcohol dehydrogenase 2 from R. toruloides fro the P2 position (F/G)

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

(F) pADH2 (G)

Expression

Bacterial

Available Since

Dec. 7, 2023

Availability

Academic Institutions and Nonprofits only

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(F) pADH2 (G)_P2 (SBE113)

Plasmid

#187628

Purpose

promoter of the gene alcohol dehydrogenase 2 from R. toruloides fro the P2 position (F/G)

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

(F) pADH2 (G)

Expression

Bacterial

Available Since

Dec. 7, 2023

Availability

Academic Institutions and Nonprofits only

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ku70 insUP+pLDP1+NAT+tNOS+ku70 insD (SBE193)

Plasmid

#195054

Purpose

integration cassette for ku70 deletion and expression of NAT gene under pLDP1 for characterization

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

NAT

Expression

Bacterial

Promoter

pLDP1

Available Since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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ku70 insUP+pLDP1+NAT+tNOS+ku70 insD (SBE193)

Plasmid

#195054

Purpose

integration cassette for ku70 deletion and expression of NAT gene under pLDP1 for characterization

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

NAT

Expression

Bacterial

Promoter

pLDP1

Available Since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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ku70 insUP+pFBA+NAT+tNOS+ku70 insD (SBE179)

Plasmid

#195053

Purpose

integration cassette for ku70 deletion and expression of NAT gene under pFBA for characterization

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

NAT

Expression

Bacterial

Promoter

pFBA

Available Since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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