We narrowed to 3,906 results for: SPL

1. pGADT7 Hsh155 K335T

   Plasmid

   #87300

   Purpose

   Encodes Y2H Gal4 activation domain for Hsh155 K335T

   Depositor

   Aaron Hoskins

   Article

   Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

   Insert

   HSH155 (HSH155 Budding Yeast)
   

   Use

   Tags

   GAL4 AD and HA

   Expression

   Bacterial and Yeast

   Mutation

   K335T

   Promoter

   Available since

   July 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGADT7 Hsh155 K335N

   Plasmid

   #87298

   Purpose

   Encodes Y2H Gal4 activation domain for Hsh155 K335N

   Depositor

   Aaron Hoskins

   Article

   Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

   Insert

   HSH155 (HSH155 Budding Yeast)
   

   Use

   Tags

   GAL4 AD and HA

   Expression

   Bacterial and Yeast

   Mutation

   K335N

   Promoter

   Available since

   July 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGADT7 Hsh155 K335E

   Plasmid

   #87297

   Purpose

   Encodes Y2H Gal4 activation domain for Hsh155 K335E

   Depositor

   Aaron Hoskins

   Article

   Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

   Insert

   HSH155 (HSH155 Budding Yeast)
   

   Use

   Tags

   GAL4 AD and HA

   Expression

   Bacterial and Yeast

   Mutation

   K335E

   Promoter

   Available since

   July 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGADT7 Hsh155 R294C

   Plasmid

   #87292

   Purpose

   Encodes Y2H Gal4 activation domain for Hsh155 R294C

   Depositor

   Aaron Hoskins

   Article

   Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

   Insert

   HSH155 (HSH155 Budding Yeast)
   

   Use

   Tags

   GAL4 AD and HA

   Expression

   Bacterial and Yeast

   Mutation

   R294C

   Promoter

   Available since

   July 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGADT7 Hsh155 E291D

   Plasmid

   #87290

   Purpose

   Encodes Y2H Gal4 activation domain for Hsh155 E291D

   Depositor

   Aaron Hoskins

   Article

   Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

   Insert

   HSH155 (HSH155 Budding Yeast)
   

   Use

   Tags

   GAL4 AD and HA

   Expression

   Bacterial and Yeast

   Mutation

   E291D

   Promoter

   Available since

   July 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pRS414 Hsh155 N295D/K335E

   Plasmid

   #87286

   Purpose

   Encodes Hsh155 N295D K335E for expression in yeast

   Depositor

   Aaron Hoskins

   Article

   Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

   Insert

   HSH155 (HSH155 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   N295D & K335E

   Promoter

   Available since

   July 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pRS414 Hsh155 R294L/D450G

   Plasmid

   #87284

   Purpose

   Encodes Hsh155 R294L D450G for expression in yeast

   Depositor

   Aaron Hoskins

   Article

   Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

   Insert

   HSH155 (HSH155 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   R294L & D450G

   Promoter

   Available since

   July 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pRS414 Hsh155 R294L/K335E

   Plasmid

   #87283

   Purpose

   Encodes Hsh155 R294L K335E for expression in yeast

   Depositor

   Aaron Hoskins

   Article

   Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

   Insert

   HSH155 (HSH155 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   R294L & K335E

   Promoter

   Available since

   July 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. GST-PRMT9 deltaTPR mutant

   Plasmid

   #67614

   Purpose

   bacterial expression of GST-PRMT9 missing residues 21-139

   Depositor

   Steven Clarke

   Article

   Hadjikyriacou et al J Biol Chem. 2015 May 15. pii: jbc.M115.

   Insert

   PRMT9 (PRMT9 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   truncation mutant missing aa 21-139

   Promoter

   tac

   Available since

   June 27, 2016

   Availability

   Academic Institutions and Nonprofits only

10. GST-PRMT9 deltaN (aa 21-350)

    Plasmid

    #67611

    Purpose

    bacterial expression of GST-PRMT9 missing residues 21-350

    Depositor

    Steven Clarke

    Article

    Hadjikyriacou et al J Biol Chem. 2015 May 15. pii: jbc.M115.

    Insert

    PRMT9 (PRMT9 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    truncation mutant missing aa 21-350

    Promoter

    tac

    Available since

    June 27, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pU6_(GLuc)_INT[S1Apt]

    Plasmid

    #68425

    Purpose

    Transient expression in mammalian cells of an "INT" construct_bearing the "S1" streptavidin aptamer, targeting the GLuc reporter. U6 promoter

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    INT construct with S1 streptavidin aptamer
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U6

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET:CACT:GFP

    Plasmid

    #60737

    Purpose

    A modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'CACT' between -11 and -8.

    Depositor

    Matthew Bennett

    Article

    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

    Insert

    GFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. SNV-memTdTomato-2AcytoGFP

    Plasmid

    #53124

    Purpose

    Retrovirus vector backbone that co-expresses GFP and a gene of interest fused to memTdTomato, driven by the Spleen Necrosis Virus promoter; for use in chicken cells

    Depositor

    Jeanette Hyer

    Article

    Venters et al Dev Dyn. 2008 Nov;237(11):3260-9. doi: 1

    Type

    Empty backbone

    Use

    Retroviral; Expression in chicken cells

    Tags

    2A-cyto-GFP and memTdTomato

    Expression

    Mutation

    Promoter

    SNV (spleen necrosis virus)

    Available since

    June 9, 2014

    Availability

    Academic Institutions and Nonprofits only

14. SNV-memTdTomato

    Plasmid

    #53123

    Purpose

    retroviral vector expressing membrane TdTomato, driven by the Spleen Necrosis Virus promoter; for use in chicken cells

    Depositor

    Jeanette Hyer

    Article

    Venters et al Dev Dyn. 2008 Nov;237(11):3260-9. doi: 1

    Insert

    memTdTomato
    

    Use

    Retroviral; Expression in chicken cells

    Tags

    Expression

    Mutation

    Promoter

    SNV (spleen necrosis virus)

    Available since

    June 9, 2014

    Availability

    Academic Institutions and Nonprofits only

15. SNV-lacZ

    Plasmid

    #53125

    Purpose

    retroviral vector expressing lacZ, driven by the Spleen Necrosis Virus promoter; for use in chicken cells

    Depositor

    Jeanette Hyer

    Article

    Venters et al Dev Dyn. 2008 Nov;237(11):3260-9. doi: 1

    Insert

    lacZ (lacZ E.coli)
    

    Use

    Retroviral; Expression in chicken cells

    Tags

    Expression

    Mutation

    Promoter

    SNV (spleen necrosis virus)

    Available since

    June 9, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pLG-Nde-Acc

    Plasmid

    #33150

    Depositor

    Michael Rosbash

    Article

    Legrain et al Cell. 1989 May 19;57(4):573-83.

    Insert

    RP51A-synthetic minimal intron (RPS17A Budding Yeast)
    

    Use

    Tags

    LacZ

    Expression

    Yeast

    Mutation

    AccI site in intron was cut/filled/ligated to cha…

    Promoter

    Available since

    Dec. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

17. hPrp16 N-terminus

    Plasmid

    #11204

    Depositor

    Robin Reed

    Article

    Zhou et al EMBO J. 1998 Apr 1. 17(7):2095-106.

    Insert

    Prp16 N-terminus (DHX38 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Amino acids 1-554

    Promoter

    Available since

    Jan. 19, 2006

    Availability

    Academic Institutions and Nonprofits only

18. Ecm2 1-143

    Plasmid

    #169923

    Purpose

    WT Ecm2 mutated to introduce stop codon after AA 143 of Ecm2

    Depositor

    Aaron Hoskins

    Article

    van der Feltz et al RNA. 2021 Feb 5. pii: rna.077727.120. do

    Insert

    Ecm2 1-143
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Ecm2 1-265

    Plasmid

    #169925

    Purpose

    WT Ecm2 mutated to introduce stop codon after AA 265 of Ecm2

    Depositor

    Aaron Hoskins

    Article

    van der Feltz et al RNA. 2021 Feb 5. pii: rna.077727.120. do

    Insert

    Ecm2 1-265
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Ecm2 1-325

    Plasmid

    #169926

    Purpose

    WT Ecm2 mutated to introduce stop codon after AA 325 of Ecm2

    Depositor

    Aaron Hoskins

    Article

    van der Feltz et al RNA. 2021 Feb 5. pii: rna.077727.120. do

    Insert

    Ecm2 1-325
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge