We narrowed to 3,403 results for: aaas

1. phage ubc nls ha 2xmcp HALO

   Plasmid

   #64540

   Purpose

   Used to label reporter mRNAs. Lentiviral expression of the MCP protein fused to HALO.

   Depositor

   Jeffrey Chao

   Article

   Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

   Insert

   MCP
   

   Use

   Lentiviral

   Tags

   HA, HALO, and NLS

   Expression

   Mammalian

   Promoter

   human ubiquitin C promoter

   Available Since

   May 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pQCascade

   Plasmid

   #140622

   Purpose

   Expresses V. cholerae TniQ, Cas8, Cas7, and Cas6 and CRISPR RNA

   Depositor

   Sheng Yang

   Article

   Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

   Insert

   VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(entry)
   

   Use

   CRISPR; Transposon

   Expression

   Bacterial

   Available Since

   July 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMD19-PBBa_J23117-sgRNA-Spr

   Plasmid

   #190793

   Purpose

   Template vector to amplify single sgRNAs or pieces for multiplexing arrays. Has flanking BsaI-sites.

   Depositor

   Paul Hudson

   Article

   Cengic et al ACS Synth Biol. 2022 Aug 15. doi: 10.102

   Insert

   sgRNA (dummy)
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   BBa_J23117

   Available Since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHsp83-NLS-HA-2xPCP-2xGFP

   Plasmid

   #71243

   Purpose

   Used to label reporter mRNAs. Drosophila expression of the PCP protein fused to EGFP.

   Depositor

   Anne Ephrussi

   Article

   Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

   Insert

   2xPCP-2xGFP
   

   Use

   P element-mediated germline transformation

   Tags

   EGFP

   Expression

   Insect

   Promoter

   Hsp83

   Available Since

   Dec. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. p-AAV-sh[SNCA]

   Plasmid

   #75437

   Purpose

   knockdown alpha-synuclein in rat neurons

   Depositor

   Edward Burton

   Article

   Zharikov et al J Clin Invest. 2015 Jul 1;125(7):2721-35

   Insert

   snca (Snca Rat)
   

   Use

   AAV and RNAi

   Expression

   Mammalian

   Available Since

   Aug. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSUPER-retro-puro-tetO-CTR1-shRNA

   Plasmid

   #53180

   Purpose

   Expresses shRNA against human CTR1 from puromycin resistance retroviral vector

   Depositor

   Christopher Counter

   Article

   Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

   Insert

   CTR1 (SLC31A1 Human)
   

   Use

   RNAi and Retroviral

   Expression

   Mammalian

   Available Since

   Sept. 19, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pDAS12230_pegRNA-PEAR-GFP(10PBS-24RT)-mCherry

   Plasmid

   #177182

   Purpose

   plasmid expressing a pegRNA targeting the PEAR-GFP plasmid along with an mCherry marker

   Depositor

   Ervin Welker

   Article

   Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

   Insert

   pegRNA targeting the PEAR-GFP plasmid
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Feb. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-3xGFP11-mem-3xControlgRNA

   Plasmid

   #224583

   Purpose

   Ubiquitous expression plasmid with CAG promoter (CMV immediate early enhancer, chicken beta actin promoter), three Control gRNAs with ribozyme self-cleavage, three membrane split GFP(11) reporter.

   Depositor

   Erica Hutchins

   Article

   Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

   Insert

   3xGFP11
   

   Use

   CRISPR

   Tags

   Membrane Localization Signal

   Mutation

   Codon optimized

   Available Since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. His_Naa80d in pTYB12

   Plasmid

   #188455

   Purpose

   Express in E. coli human Naa80 delta (actin's N-terminal acetyl transferase) with N-terminal intein-chitin binding domain tag and 6xHis tag.

   Depositor

   Roberto Dominguez

   Article

   Ceron et al Proc Natl Acad Sci U S A. 2022 Oct 11;11

   Insert

   Naa80 (NAA80 Human)
   

   Tags

   intein-CBD, 6xHis

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available Since

   Nov. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. gRNA-his-HYB

    Plasmid

    #64333

    Purpose

    his3 deletion gRNA cassette carried by pRS42H

    Depositor

    Yong-Su Jin

    Article

    Zhang et al Appl Environ Microbiol. 2014 Dec;80(24):

    Insert

    gBlock product of his3 deletion gRNA cassette
    

    Expression

    Yeast

    Available Since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pCAG-nucGFP11-3xControlgRNA

    Plasmid

    #224568

    Purpose

    Ubiquitous expression plasmid, CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), three Control gRNAs with ribozyme self-cleavage sites, and nuclear split GFP(11) reporter.

    Depositor

    Erica Hutchins

    Article

    Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

    Insert

    GFP11-H2B
    

    Use

    CRISPR

    Tags

    Histone H2B (nuclear localization)

    Mutation

    Codon optimized

    Available Since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBB75-ncRNA

    Plasmid

    #194087

    Purpose

    Expresses Ec86 ncRNA in Escherichia coli

    Depositor

    Tingting Zou

    Article

    Wang et al Nat Microbiol. 2022 Sep;7(9):1480-1489.

    Insert

    Ec86 ncRNA
    

    Promoter

    T7

    Available Since

    July 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. shLacZ

    Plasmid

    #42559

    Depositor

    William Hahn

    Article

    Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

    Insert

    shLacZ
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Feb. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

14. pLKO.1-sh-mSlug-4

    Plasmid

    #40648

    Depositor

    Bob Weinberg

    Article

    Guo et al Cell. 2012 Mar 2;148(5):1015-28.

    Insert

    sh-mSlug-4 (Snai2 Mouse)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Sept. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

15. pTC364

    Plasmid

    #91213

    Purpose

    protoplast vector expressing 2 gRNAs targeting tomato ARF8A (CmYLCV promoter, Csy4 processing)

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    2 gRNAs targeting tomato ARF8A
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pYJ34-PB-CRISPR-BANCR-E1-gRNA

    Plasmid

    #131077

    Purpose

    long non-coding RNA BANCR knock out

    Depositor

    Xiaojun Lian

    Article

    Jiang et al Bioact Mater. 2022 Feb 7;14:313-320. doi

    Insert

    BANCR Exon1 gRNA (BANCR Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    May 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLKO-STAG2 shRNA 3782

    Plasmid

    #31979

    Depositor

    Todd Waldman

    Article

    Solomon et al Science. 2011 Aug 19;333(6045):1039-43.

    Insert

    STAG2 (STAG2 Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Aug. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

18. pLKO.1-sh-mSlug-3

    Plasmid

    #40647

    Depositor

    Bob Weinberg

    Article

    Guo et al Cell. 2012 Mar 2;148(5):1015-28.

    Insert

    sh-mSlug-3 (Snai2 Mouse)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Sept. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

19. pSUPER IKKbeta

    Plasmid

    #26209

    Depositor

    Tom Maniatis

    Article

    Fitzgerald et al Nat Immunol. 2003 May;4(5):491-6.

    Insert

    pSUPER IKK beta (IKBKB Human)
    

    Use

    RNAi

    Available Since

    Aug. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

20. pHS1182

    Plasmid

    #205964

    Purpose

    NZ_CP025815 DinG HNH crRNA expression in pCOLADuet-1

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

    Insert

    DinG HNH crRNA
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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