We narrowed to 14,273 results for: cas9

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SpCas9-CBE6c

Plasmid

#215821

Purpose

Express CBE6c (with SpCas9) in mammalian cells

Depositor

David Liu

Article

Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

Insert

CBE6c-SpCas9-2xUGI (cas9 )

Expression

Mammalian

Available Since

March 8, 2024

Availability

Academic Institutions and Nonprofits only

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pMS59

Plasmid

#215680

Purpose

Cas9 + guide plasmid targeting ChrI split hygromycinR landing pad

Depositor

Patrick Phillips

Article

Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

Insert

eef-1A.1p::Cas9 + U6p::GTTTGAGTAGAGCACTCAGG

Use

CRISPR

Expression

Worm

Available Since

March 21, 2024

Availability

Academic Institutions and Nonprofits only

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pLEW100v5-BSD-FLAG-La-Cas9

Plasmid

#245102

Purpose

Inserts tetracycline-inducible codon optimized, nuclceus targeted FLAG-Cas9 into T. brucei cells at the rDNA spacer

Depositor

Monica Mugnier

Article

Smith et al Nature. 2026 Apr 8. doi: 10.1038/s41586-

Insert

3xFLAG-La-Cas9

Use

CRISPR

Tags

3xFLAG-La

Mutation

codon optimized for T. brucei

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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UAS-u(XXL)Cas9

Plasmid

#127387

Purpose

Expresses Cas9 at very low level

Depositor

Michael Boutros

Article

Port et al Elife. 2020 Feb 13;9. pii: 53865. doi: 1

Insert

uORF(XXL)-Cas9

Use

CRISPR

Expression

Insect

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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UAS-u(L)Cas9

Plasmid

#127385

Purpose

Expresses Cas9 at low level

Depositor

Michael Boutros

Article

Port et al Elife. 2020 Feb 13;9. pii: 53865. doi: 1

Insert

uORF(L)-Cas9

Use

CRISPR

Expression

Insect

Availability

Academic Institutions and Nonprofits only

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pYPQ-CBE-Cas9n-Act3.0

Plasmid

#178955

Purpose

It consists of a Cas9 nickase (D10A) fused with a cytidine deaminase (A3A/Y130F) and MS2-SunTag-activators (ScFv-sfGFP-2xTAD), enabling simultaneous C to T conversion and gene activation.

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2022 May;8(5):513-525. doi:

Insert

hA3A-zCas9-UGI-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter

Use

CRISPR

Expression

Plant

Available Since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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PRE-Hsp70BbCas9_1.0

Plasmid

#190795

Purpose

PRE-Hsp70Bb-Cas9-T2A-eGFP; heat-shock inducible Cas9-T2A-eGFP expression regulated by upstream Polycomb response elements. Step 1 in cloning process

Depositor

Omar Akbari

Article

Warsinger-Pepe et al G3 (Bethesda). 2023 Apr 11;13(4):jkad024

Insert

PRE-Hsp70Bb-Cas9-T2A-eGFP

Use

CRISPR

Expression

Insect

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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A3Bi-Cas9n-UGI

Plasmid

#119135

Purpose

Base editor made from full-length APOBEC3B with an L1 intron to prevent self-restriction

Depositor

Reuben Harris

Article

Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

Insert

APOBEC3B L1 intron-Cas9 nickase-UGI

Use

CRISPR

Mutation

L1 intron insertion

Promoter

CMV

Available Since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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pIF1079

Plasmid

#237445

Purpose

Expression SpCas9 and Efe1-RT from bidirectional CAG promoters. SpCas9 sgRNA and Efe1 non-coding expression is driven by U6 and H1 respecitvely.

Depositor

IIya Finkelstein

Article

Buffington et al Nat Biotechnol. 2025 Oct 23. doi: 10.103

Inserts

SpCas9
Efe1-RT
EMX1 sgRNA
Efe1-ncRNA targeting EMX1

Tags

3xFLAG

Expression

Mammalian

Promoter

CAG, H1, and U6

Available Since

June 24, 2025

Availability

Academic Institutions and Nonprofits only

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PRE-Hsp70BbCas9_1.2

Plasmid

#190796

Purpose

gypsyPRE-Hsp70Bb-Cas9-T2A-eGFP; heat-shock inducible Cas9-T2A-eGFP expression regulated by upstream Polycomb response elements with one gypsy insulator element. Step 2 in cloning process.

Depositor

Omar Akbari

Article

Warsinger-Pepe et al G3 (Bethesda). 2023 Apr 11;13(4):jkad024

Insert

gypsyPRE-Hsp70Bb-Cas9-T2A-eGFP

Expression

Insect

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-v2-ALDH1A3gRNA2

Plasmid

#189747

Purpose

The construct was used for expression of gRNA and Cas9 for knocking out of the ALDH1A3 gene.

Depositor

Robert Sobol

Article

Li et al Commun Biol. 2021 Dec 21;4(1):1420. doi:

Insert

Cas9, ALDH1A3 gRNA

Use

CRISPR

Promoter

U6

Available Since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-v2-ALDH1A3gRNA1

Plasmid

#189746

Purpose

The construct was used for expression of gRNA and Cas9 for knocking out of the ALDH1A3 gene.

Depositor

Robert Sobol

Article

Li et al Commun Biol. 2021 Dec 21;4(1):1420. doi:

Insert

Cas9, ALDH1A3 gRNA

Use

CRISPR

Promoter

U6

Available Since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-VRER-P2A-Puro

Plasmid

#110849

Purpose

Lentiviral vector for constitutive expression of Cas9-VRER (not codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-VRER

Use

Lentiviral

Tags

3X FLAG

Mutation

D1135V, G1218R, R1335E, T1337R

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-HF1-P2A-Puro

Plasmid

#110850

Purpose

Lentiviral vector for constitutive expression of Cas9-HF1 (not codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-HF1

Use

Lentiviral

Tags

3X FLAG

Mutation

N497A, R661A, Q695A, Q926A

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-VQR-PGK-Puro

Plasmid

#110855

Purpose

Lentiviral vector for constitutive expression of Cas9-VQR (not codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-VQR

Use

Lentiviral

Tags

3X FLAG

Mutation

D1135V, R1335Q, T1337R

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-VRER-PGK-Puro

Plasmid

#110856

Purpose

Lentiviral vector for constitutive expression of Cas9-VRER (not codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-VRER

Use

Lentiviral

Tags

3X FLAG

Mutation

D1135V, G1218R, R1335E, T1337R

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-VRER-P2A-GFP-PGK-Puro

Plasmid

#110862

Purpose

Lentiviral vector for constitutive expression of Cas9-VRER-P2A-GFP (not codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-VRER

Use

Lentiviral

Tags

3X FLAG

Mutation

D1135V, G1218R, R1335E, T1337R

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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M-tdTom-NM

Plasmid

#48679

Purpose

Mammalian tdTomato activation reporter for NM with GTCCCCTCCACCCCACAGTG protospacer

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

TAL binding site w/ GTCCCCTCCACCCCACAGTG protospacer, NM-PAM, and tdTomato reporter. Compatible with N. meningitidis Cas9

Use

CRISPR

Promoter

Sp6

Available Since

Oct. 22, 2013

Availability

Academic Institutions and Nonprofits only
pTE_21 - pIVT_CMV-synUTR-inactT7-NLS-Cas9-NLS-XTEN-BLM(1-193)-NLS-HbaUTR

Plasmid

#252017

Purpose

In vitro transcription of Cas9-BLM(2-194) TruEditor for mRNA generation

Depositor

Neville Sanjana

Article

Burrell et al bioRxiv 2026.02.19.706875

Insert

Cas9-BLM(2-194)

Use

CRISPR

Expression

Mammalian

Mutation

NA

Available Since

March 4, 2026

Availability

Academic Institutions and Nonprofits only

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pFZ1036

Plasmid

#250676

Purpose

IPT along with RUBY for soybean genome editing

Depositor

Feng Zhang

Article

Alok et al Plant Physiol. 2025 Dec 10:kiaf640. doi:

Insert

IPT, Cas9, RUBY

Use

CRISPR

Expression

Plant

Promoter

CaMV35, AtUbi10

Available Since

Feb. 6, 2026

Availability

Academic Institutions and Nonprofits only

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