We narrowed to 6,299 results for: chia

1. pBbA5c-MevT-MBI (JBEI-3085)

   Plasmid

   #87950

   Purpose

   the overproduction of the universal building blocks to make isoprenoids (i.e. IPP and DMAPP).

   Depositor

   Taek Soon Lee

   Article

   Alonso-Gutierrez et al Metab Eng. 2013 May 29;19C:33-41. doi: 1

   Insert

   MevT-MBI
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pC28-LIC-sfGFP-His

   Plasmid

   #165479

   Purpose

   pET based vector for protein expression in E.coli for targets fused with C-terminal superfolder GFP and His tag

   Depositor

   Renato Bruni

   Article

   Bruni et al Curr Protoc Protein Sci. 2013 Nov 5;74:2

   Type

   Empty backbone

   Use

   Tags

   TEV-GFP-6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLG016 (ApeA)

   Plasmid

   #157894

   Purpose

   Expresses the ApeA defense system

   Depositor

   Feng Zhang

   Article

   Gao et al Science. 2020 Aug 28;369(6507):1077-1084

   Insert

   ApeA (putative HEPN RNase)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLRD16

   Plasmid

   #208268

   Purpose

   Production of syn-stemar-13-ene in E. coli MEV20

   Depositor

   Christopher Voigt

   Article

   Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

   Inserts

   tHMGR
   osCPS4
   osKSL8
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pT2SK

   Plasmid

   #59383

   Purpose

   Template plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a kanamycin resistance gene.

   Depositor

   Shelley Copley

   Article

   Kim et al BMC Biotechnol. 2014 Sep 25;14(1):84.

   Insert

   TT-ISceI-kan
   

   Use

   Template

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRecE564T

   Plasmid

   #226825

   Purpose

   Expressing RecE*T controled by Ptrc promoter

   Depositor

   Sheng Yang

   Article

   Wang et al ACS Synth Biol. 2025 May 8. doi: 10.1021

   Insert

   recE*T
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAJM.683

   Plasmid

   #108531

   Purpose

   BetIAM + PBetI-YFP Reporter. Cho sensor for use in non-Marionette strain.

   Depositor

   Christopher Voigt

   Article

   Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

   Insert

   BetIAM + PBetI-YFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   BetI: S115I

   Promoter

   Available since

   Aug. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pT2SC

   Plasmid

   #59382

   Purpose

   Template plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a chloramphenicol resistance gene.

   Depositor

   Shelley Copley

   Article

   Kim et al BMC Biotechnol. 2014 Sep 25;14(1):84.

   Insert

   TT-ISceI-cat
   

   Use

   Template

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMUT2-pLac-mCherry

   Plasmid

   #192859

   Purpose

   pMUT2 based vector for sensing lactic acid in E. coli Nissle 1917

   Depositor

   Morten Sommer

   Article

   Vaaben et al ACS Synth Biol. 2022 Dec 16;11(12):4184-

   Insert

   lldR operon
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDsEndoNA2

    Plasmid

    #174728

    Purpose

    Expresses DsRed-endoNA2 fusion protein for detection of polysialic acid. The endoNA2 is a catalytically inactive form of endosialidase from bacteriophage PK1A2.

    Depositor

    Jukka Finne

    Article

    Lehti et al Nat Commun. 2017 Dec 4;8(1):1915. doi: 1

    Insert

    DsRed-Monomer
    

    Use

    Tags

    Histag

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pIBA2-Int-SpyTag

    Plasmid

    #198038

    Purpose

    Expression plasmid for producing SpyTagged stalled secretion variant of intimin

    Depositor

    Jack Leo

    Article

    Duodu et al Methods Mol Biol. 2024;2778:53-63. doi:

    Insert

    eaeA
    

    Use

    Tags

    SpyTag

    Expression

    Bacterial

    Mutation

    lacks native signal peptide (amino acids 1-39)

    Promoter

    tet

    Available since

    March 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28a T7pCONS sfGFP

    Plasmid

    #154462

    Purpose

    pET28a with a design flaw in the T7 promoter fixed. Contains the coding sequence for sfGFP

    Depositor

    Daniel Daley

    Article

    Shilling et al Commun Biol. 2020 May 7;3(1):214. doi: 1

    Insert

    super folder GFP
    

    Use

    Tags

    His-thrombin

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pQE30del

    Plasmid

    #106902

    Purpose

    pQE30del is conditionally replicating empty vector. Replication of the plasmid is inhibited in presense of IPTG or lactose. pQE30del can be used for construction of any E. coli helper plasmids.

    Depositor

    Sergey Sineoky

    Article

    Bubnov et al J Microbiol Methods. 2018 Jun 7. pii: S0

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAJM.715

    Plasmid

    #108516

    Purpose

    PTac-YFP Reporter. IPTG sensor for use in Marionette strain.

    Depositor

    Christopher Voigt

    Article

    Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

    Insert

    PTac-YFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTJV1ScKm-rpoB

    Plasmid

    #166980

    Purpose

    Generates ssDNA in vivo targeting E. coli rpoB for recombineering w/ Beta recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; npt2 for kanamycin resistance

    Depositor

    Christopher Reisch

    Article

    Ellington et al Synth Biol (Oxf). 2022 May 3;7(1):ysac00

    Insert

    NeoR/KanR
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pet28a-ClbB-NRPS-NHis

    Plasmid

    #49217

    Purpose

    NRPS module of ClbB containing CAT domains with N-terminal 6His tag

    Depositor

    Emily Balskus

    Article

    Brotherton et al J Am Chem Soc. 2013 Mar 6;135(9):3359-62

    Insert

    ClbB-NRPS
    

    Use

    Tags

    6x His tag and T7 tag

    Expression

    Bacterial

    Mutation

    contains aa4-1080 of reference sequence NP_754362…

    Promoter

    T7

    Available since

    March 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCH1 - pBait-empty

    Plasmid

    #174663

    Purpose

    arabinose-induced synthesis of an RNA containing a single MS2 hairpin. Used as a three-hybrid control. For cloning purposes, see plasmid #174662.

    Depositor

    Katherine Berry

    Article

    Pandey et al Nucleic Acids Res. 2020 May 7;48(8):4507

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMDICI

    Plasmid

    #51652

    Purpose

    E coli genetic modification

    Depositor

    Sheng Yang

    Article

    Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

    Insert

    CmR
    

    Use

    Genetic modification

    Tags

    Expression

    Mutation

    Promoter

    unknown

    Available since

    July 7, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pAGD236

    Plasmid

    #219497

    Purpose

    pCDF-Duet deriviative with sfGFP flanked by 2 BsaI sites downstream of the first T7 promoter and independent msr and RT downstream of the second T7 promoter

    Depositor

    Seth Shipman

    Article

    Gonzalez-Delgado et al bioRxiv [Preprint]. 2023 Jul 17:2023.07.

    Insert

    sfGFP, Eco1 msr, Eco1 RT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. p5E-QUASM1

    Plasmid

    #155114

    Purpose

    5' entry vector containing QUASM1 promoter element.

    Depositor

    Bret Pearson

    Article

    Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

    Insert

    QUASM1
    

    Use

    Gateway 5' entry vector

    Tags

    Expression

    Mutation

    Promoter

    QUASM1-E1b

    Available since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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