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pCDNA3 14 mut B-Myb

Plasmid

#25967

Depositor

Rob Lewis

Article

Johnson et al J Biol Chem. 2002 Feb 8. 277(6):4088-97.

Insert

B-Myb (Mybl2 Mouse)

Tags

Flag

Expression

Bacterial

Mutation

Changed threonines 267, 408, 443, 447 490, 497, 5…

Available Since

Sept. 20, 2010

Availability

Academic Institutions and Nonprofits only
pDONR221-rap1-K9R

Plasmid

#84527

Purpose

This plasmid can be used as a donor for subcloning into Gateway destination vectors.

Depositor

Jorrit Enserink

Article

Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

Insert

RAP1 (RAP1 Budding Yeast)

Use

Gateway donor plasmid

Mutation

K(43,61,69,117,240,246,527,582,651)R

Promoter

none

Available Since

Jan. 17, 2017

Availability

Academic Institutions and Nonprofits only
WZLneo-PPARγ2

Plasmid

#34566

Depositor

Kai Ge

Article

Wang et al Proc Natl Acad Sci U S A. 2010 Apr 20. 1

Insert

PPARγ2 (Pparg Mouse)

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Promoter

5'LTR

Available Since

Jan. 25, 2012

Availability

Academic Institutions and Nonprofits only
pXGFPC-5 Pxyl-dcas9 (Spy)

Plasmid

#133316

Purpose

expresses dcas9 from Streptococcus pyogenes; repressed with 0.2% glucose, induced with 0.3% xylose; for homologous recombination at the xylose locus in Caulobacter crescentus; tetracycline resistance

Depositor

Michael Laub

Article

Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

Insert

dcas9 (Streptococcus pyogenes)

Use

CRISPR

Promoter

xylose

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2/V5 hsa-mir-193b

Plasmid

#26318

Depositor

David Bartel

Article

Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

Insert

hsa-mir-193b (MIR193B Human)

Expression

Mammalian

Available Since

Sept. 29, 2010

Availability

Academic Institutions and Nonprofits only
pJL1-EPA-DNNNS-DQNRT

Plasmid

#128397

Purpose

In vitro expression of P. aeruginosa exotoxin A with DNNNS and DQNRT internal glycosylation sites

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

Insert

Exotoxin A

Tags

6xHis tag

Expression

Bacterial

Mutation

242DNNNS246 and 384DQNRT388 engineered glycosylat…

Promoter

T7

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR4_FLAG/HA_FUS_His6

Plasmid

#26365

Depositor

Thomas Tuschl

Article

Spitzer et al Genes Chromosomes Cancer. 2011 May . 50(

Insert

FUS (FUS Human)

Use

Gateway Cloning

Tags

FLAG/HA and His6

Mutation

delta 527 (stop codon)

Available Since

Oct. 18, 2010

Availability

Academic Institutions and Nonprofits only
pBCV

Plasmid

#62659

Purpose

Mammalian expression of FLPo recombinase from the broadly active CAG promoter/enhancer

Depositor

Masato Ohtsuka

Article

Ohtsuka et al BMC Genomics. 2015 Apr 9;16:274. doi: 10

Insert

FLPo

Expression

Mammalian

Available Since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only

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pLPC-MYC.Mm.RAP1.delta.MYB

Plasmid

#184660

Purpose

Retroviral expression of mouse RAP1 delta MYB

Depositor

Agnel Sfeir

Article

Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

Insert

rap1

Use

Retroviral

Tags

MYC

Expression

Mammalian

Mutation

rap1 delta MYB

Promoter

CMV

Available Since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

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pLPC-MYC.Mm.RAP1.delta.COIL

Plasmid

#184659

Purpose

Retroviral expression of mouse RAP1 delta COIL

Depositor

Agnel Sfeir

Article

Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

Insert

pLPC

Use

Retroviral

Tags

MYC

Expression

Mammalian

Mutation

rap1 delta COIL

Promoter

CMV

Available Since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

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pLPC-MYC.Mm.RAP1.delta.BRCT

Plasmid

#184658

Purpose

Retroviral expression of mouse Rap1 delta BRCT

Depositor

Agnel Sfeir

Article

Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

Insert

RAP1

Use

Retroviral

Tags

MYC

Expression

Mammalian

Mutation

rap1 delta BRCT

Promoter

CMV

Available Since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

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pLPC-MYC.Mm.RAP1.delta.RCT

Plasmid

#184661

Purpose

Retroviral expression of mouse RAP1 delta RCT

Depositor

Agnel Sfeir

Article

Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

Insert

rap1

Use

Retroviral

Tags

MYC

Expression

Mammalian

Mutation

rap1 delta RCT

Promoter

CMV

Available Since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

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pJL1-TTlight-4xDQNAT

Plasmid

#128394

Purpose

In vitro expression of tetanus toxin light chain with C terminal 4xDQNAT glycosylation sites

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

Insert

Tetanus toxin light chain domain

Tags

4xDQNAT glycosylation tag and 6xHis tag

Expression

Bacterial

Mutation

Inactivating E234A mutation

Promoter

T7

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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BII-ChBtW-eSpCas9

Plasmid

#133370

Purpose

Piggybac vector for constitutive expression

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Insert

eSpCas9

Tags

Flag

Expression

Mammalian

Mutation

eSpCas9 mutations

Promoter

CMV enhancer and hEf1a (CpG free)

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pG.Lantern-SM50-GFP

Plasmid

#135593

Purpose

Expression of SM50 Promoter-GFP mRNA for microinjection to visualize SM50 promoter activity in S. purpuratus

Depositor

Koji Akasaka, Mamiko Yajima

Article

Yajima et al Dev Genes Evol. 2007 Apr;217(4):331-6. d

Insert

SM50 promoter (SM50 S. purpuratus)

Use

In vitro transcription (mrna synthesis)

Tags

Green Lantern GFP

Promoter

CMV

Available Since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-RAP1

Plasmid

#84525

Purpose

This plasmid can be used as a donor for subcloning into Gateway destination vectors.

Depositor

Jorrit Enserink

Article

Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

Insert

RAP1 (RAP1 Budding Yeast)

Use

Gateway donor plasmid

Mutation

no stop codon

Promoter

none

Available Since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pTrc99S-ssDsbA-PD-4xDQNAT

Plasmid

#128399

Purpose

Periplasmic expression of H. influenzae protein D with C terminal 4xDQNAT glycosylation sites in E. coli

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

Insert

H. influenzae protein D

Tags

4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…

Expression

Bacterial

Promoter

trc

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pTrc99S-ssDsbA-PorA-4xDQNAT

Plasmid

#128400

Purpose

Periplasmic expression of N. meningitidis PorA with C terminal 4xDQNAT glycosylation sites in E. coli

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

Insert

N. meningitidis PorA

Tags

4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…

Expression

Bacterial

Promoter

trc

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pBER

Plasmid

#62662

Purpose

Donor vector for tdTomato knock-in via landing pad

Depositor

Masato Ohtsuka

Article

Ohtsuka et al BMC Genomics. 2015 Apr 9;16:274. doi: 10

Inserts

tdTomato
hygromycin

Use

Cre/Lox

Available Since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only

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pBGX

Plasmid

#62666

Purpose

Donor vector for Dre knock-in via landing pad

Depositor

Masato Ohtsuka

Article

Ohtsuka et al BMC Genomics. 2015 Apr 9;16:274. doi: 10

Insert

Dre

Use

Cre/Lox

Available Since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only

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