We narrowed to 24,831 results for: nes

1. pGEX6P1‐Afu rnhB

   Plasmid

   #108694

   Purpose

   For expression in E coli, and affinity purification of N-terminally GST-tagged Archaeoglobus fulgidus RNase HII

   Depositor

   Martin Reijns, Andrew Jackson

   Article

   Reijns et al J Biol Chem. 2011 Mar 25;286(12):10530-9

   Insert

   rnhB (AF_RS03165 Archaeoglobus fulgidus)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   April 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAG415-ADH1p-HA-rap1-K9R

   Plasmid

   #84531

   Purpose

   This plasmid can be used to express HA-tagged Rap1-K9R from the ADH promoter in yeast

   Depositor

   Jorrit Enserink

   Article

   Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

   Insert

   RAP1 (RAP1 Budding Yeast)
   

   Use

   Tags

   HA

   Expression

   Yeast

   Mutation

   K(43,61,69,117,240,246,527,582,651)R

   Promoter

   ADH1

   Available since

   Jan. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

3. pAG415-GPDp-EGFP-rap1-K3NR

   Plasmid

   #84533

   Purpose

   This plasmid can be used to express EGFP-tagged Rap1K3NR from the GDP promoter in yeast

   Depositor

   Jorrit Enserink

   Article

   Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

   Insert

   RAP1 (RAP1 Budding Yeast)
   

   Use

   Tags

   EGFP

   Expression

   Yeast

   Mutation

   K(43,117,118)R

   Promoter

   GPD

   Available since

   Dec. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

4. pAG415-GPDp-EGFP-rap1-K3CR

   Plasmid

   #84534

   Purpose

   This plasmid can be used to express EGFP-tagged Rap1K3CR from the GDP promoter in yeast

   Depositor

   Jorrit Enserink

   Article

   Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

   Insert

   RAP1 (RAP1 Budding Yeast)
   

   Use

   Tags

   EGFP

   Expression

   Yeast

   Mutation

   K(462,463,651)R

   Promoter

   GPD

   Available since

   Dec. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pAG415-GPDp-EGFP-rap1-K9R

   Plasmid

   #84535

   Purpose

   This plasmid can be used to express EGFP-tagged Rap1K9R from the GDP promoter in yeast

   Depositor

   Jorrit Enserink

   Article

   Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

   Insert

   RAP1 (RAP1 Budding Yeast)
   

   Use

   Tags

   EGFP

   Expression

   Yeast

   Mutation

   K(43,61,69,117,240,246,527,582,651)R

   Promoter

   GPD

   Available since

   Dec. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pDONR221-rap1-K462-463-651R

   Plasmid

   #84564

   Purpose

   This plasmid can be used as a donor for subcloning into Gateway destination vectors.

   Depositor

   Jorrit Enserink

   Article

   Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

   Insert

   RAP1 (RAP1 Budding Yeast)
   

   Use

   Gateway donor plasmid

   Tags

   Expression

   Mutation

   K462-463-651R

   Promoter

   none

   Available since

   Nov. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR221-rap1-K43-117-118R

   Plasmid

   #84565

   Purpose

   This plasmid can be used as a donor for subcloning into Gateway destination vectors.

   Depositor

   Jorrit Enserink

   Article

   Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

   Insert

   RAP1 (RAP1 Budding Yeast)
   

   Use

   Gateway donor plasmid

   Tags

   Expression

   Mutation

   K43-117-118R

   Promoter

   none

   Available since

   Oct. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3.2/V5 hsa-mir-220a

   Plasmid

   #26314

   Depositor

   David Bartel

   Article

   Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

   Insert

   hsa-mir-220a (MIR220A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 29, 2010

   Availability

   Academic Institutions and Nonprofits only

9. pBa.GFP-KIF13AS1371D

   Plasmid

   #224417

   Purpose

   Mammalian expression of KIF 13a S1371D

   Depositor

   Marvin Bentley

   Article

   Mendoza et al Mol Biol Cell. 2024 Jun 1;35(6):ar81. do

   Insert

   KIF 13a S1371D (Kif13a Mouse)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   S1371D

   Promoter

   Chicken Beta actin

   Available since

   Nov. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBa.GFP-KIF13AS1371A

    Plasmid

    #224409

    Purpose

    Mammalian expression of KIF 13a S1371A

    Depositor

    Marvin Bentley

    Article

    Mendoza et al Mol Biol Cell. 2024 Jun 1;35(6):ar81. do

    Insert

    KIF 13a S1371A (Kif13a Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    S1371A

    Promoter

    Chicken Beta actin

    Available since

    Sept. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBa.GFP-KIF13AWLDLE

    Plasmid

    #224410

    Purpose

    Mammalian expression of KIF 13a WLDLE

    Depositor

    Marvin Bentley

    Article

    Mendoza et al Mol Biol Cell. 2024 Jun 1;35(6):ar81. do

    Insert

    KIF 13a WLDLE (Kif13a Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    WLDLE

    Promoter

    Chicken Beta actin

    Available since

    Sept. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEGFP-C1-IFT57

    Plasmid

    #218728

    Purpose

    Expresses N-terminally EGFP-tagged IFT57 in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Funabashi et al J Cell Biol. 2018 Aug 6;217(8):2867-2876

    Insert

    IFT57 (IFT57 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-hSyn-UDP1.0-WPRE

    Plasmid

    #220849

    Purpose

    AAV virus packaging plasmid for neuronal expression of an extracellular UDP sensor (UDP1.0)

    Depositor

    Yulong Li

    Article

    Umpierre et al Neuron. 2024 Apr 10:S0896-6273(24)00195-

    Insert

    UDP1.0
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    human Synapsin (hSyn)

    Available since

    July 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLPC-Flag-BirA-Linker-Myc-mRap1

    Plasmid

    #184644

    Purpose

    Retroviral expression of BirA-mRap1

    Depositor

    Agnel Sfeir

    Article

    Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

    Insert

    BirA-rap1
    

    Use

    Retroviral

    Tags

    BirA FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. puc19_N_tag

    Plasmid

    #186650

    Purpose

    Empty donor plasmid.

    Depositor

    Astrid Haase

    Article

    Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

    Insert

    N-terminal 3xFlag-3xHA Tag
    

    Use

    Tags

    3xFlag-3xHA

    Expression

    Insect

    Mutation

    Promoter

    Available since

    July 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLPC-Flag-BirA-Linker-Myc-mRap1-I312R

    Plasmid

    #184645

    Purpose

    Retroviral expression of BirA Rap1 I312R

    Depositor

    Agnel Sfeir

    Article

    Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

    Insert

    BirA-rap1-I312R
    

    Use

    Retroviral

    Tags

    BirA FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJCC_164 SpCas9 R1333A/R1335A

    Plasmid

    #179528

    Purpose

    For bacterial expression of SpCas9 R1333A/R1335A (PAM-binding arginines mutant) with an N-terminal His-MBP tag

    Depositor

    Jennifer Doudna

    Article

    Cofsky et al Nat Struct Mol Biol. 2022 Apr;29(4):395-

    Insert

    SpCas9 R1333A/R1335A
    

    Use

    Tags

    10X His, TEV protease cleavage site, and maltose-…

    Expression

    Bacterial

    Mutation

    changed arginines 1333 and 1335 to alanine

    Promoter

    Available since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMP71-mGFP-llo190-mc38minor

    Plasmid

    #174604

    Purpose

    expresses palmitoylated GFP with a C terminal fusion of the LLO190 and minigenes of 3 neoantigens from the MC38 tumor in genes Aatf, irgq and cpne1

    Depositor

    Stanley Riddell

    Article

    Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

    Insert

    palmitoyl-GFP C-term fusion LLO190 and minigenes of 3 neoantigens from MC38 tumor in genes Aatf irgq cpne1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX458-Ef1a-Cas9-GFP (gRNA: DHS-4D-3)

    Plasmid

    #159664

    Purpose

    CRISPR/Cas9 expressing plasmid containing the gRNA targeting an enhancer of mouse Otx2.

    Depositor

    Joseph Brzezinski

    Article

    Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Ef1a

    Available since

    June 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pX458-Ef1a-Cas9-GFP (gRNA: DHS-4D-2)

    Plasmid

    #159663

    Purpose

    CRISPR/Cas9 expressing plasmid containing the gRNA targeting an enhancer of mouse Otx2.

    Depositor

    Joseph Brzezinski

    Article

    Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Ef1a

    Available since

    March 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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