We narrowed to 24,393 results for: nes

1. pLPC-FLAG.Mm.KAT5

   Plasmid

   #184647

   Purpose

   Retroviral expression of mouse Kat5

   Depositor

   Agnel Sfeir

   Article

   Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

   Insert

   Kat5 (Kat5 Mouse)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKRG3-mU6-PUb-3xFLAG-hSpCas9

   Plasmid

   #162161

   Purpose

   Expresses 3xFLAG tagged hSpCas9 (Ae. aegypti PUb promoter) with cloning site for sgRNAs (Ae. aegypti U6 promoter)

   Depositor

   Charles Rice

   Article

   Rozen-Gagnon et al Sci Rep. 2021 Jan 12;11(1):736. doi: 10.

   Inserts

   hSpCas9
   sgRNA
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Insect

   Mutation

   Promoter

   Ae. aegypti PUb and Ae. aegypti U6

   Available since

   Jan. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. TOP2A sgRNA1

   Plasmid

   #138190

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human TOP2A

   Depositor

   Wen Xue

   Article

   Kwan et al Hepatology. 2019 Jun 12. doi: 10.1002/he

   Insert

   TOP2A (TOP2A Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   March 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. TOP2A sgRNA2

   Plasmid

   #138191

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human TOP2A

   Depositor

   Wen Xue

   Article

   Kwan et al Hepatology. 2019 Jun 12. doi: 10.1002/he

   Insert

   TOP2A (TOP2A Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   March 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV_mU6-sgASCL1_hU6-sgRNA_hUbC-PuroR-P2A-GFP

   Plasmid

   #162337

   Purpose

   Lentiviral expression of sgASCL1 paired with a second S. pyogenes sgRNA with a GFP-P2A-PuroR selection marker

   Depositor

   Charles Gersbach

   Article

   Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

   Insert

   S. pyogenes sgRNA
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6; mU6

   Available since

   Feb. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. SpIMPDH

   Plasmid

   #206980

   Purpose

   Streptococcus pyogenes Inosine 5′-Monophosphate Dehydrogenase (SpIMPDH, EC. 1.1.1.205). Contains His6 tag with TEV protease cleavage site in pET-28b(+) plasmid for protein expression.

   Depositor

   Vern Schramm

   Article

   Glockzin et al Biochemistry. 2022 Oct 4;61(19):2088-210

   Insert

   SpIMPDH (guaB Streptococcus pyogenes)
   

   Use

   Tags

   His tag (with TEV cleavage site)

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLCKO_LacZ_sgRNA

   Plasmid

   #74179

   Purpose

   lentiviral vector expressing sgRNA targeting LacZ

   Depositor

   Jason Moffat

   Article

   Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10

   Insert

   LacZ sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 Promoter

   Available since

   March 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCb.CAG-HaloTag-GA-ZF-KrabA-KIF5B 376-963

   Plasmid

   #134612

   Purpose

   Mammalian expression of mouse KIF5B 376-963

   Depositor

   Marvin Bentley

   Article

   Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

   Insert

   KIF5B (Kif5b Mouse)
   

   Use

   Tags

   Halo

   Expression

   Mammalian

   Mutation

   WT with deletion of the 375 N-terminal residues.

   Promoter

   Chicken Beta actin

   Available since

   April 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBa.GFP-KIF13A tail 361-1749

   Plasmid

   #134622

   Purpose

   Mammalian expression of mouse KIF13A 361-1749

   Depositor

   Marvin Bentley

   Article

   Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

   Insert

   KIF13A (Kif13a Mouse)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   WT with deletion of the 360 N-terminal residues.

   Promoter

   Chicken Beta actin

   Available since

   Jan. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBa.GFP-KIF13B tail 442-1843

    Plasmid

    #134623

    Purpose

    Mammalian expression of mouse KIF13B 442-1843

    Depositor

    Marvin Bentley

    Article

    Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

    Insert

    KIF13B (Kif13b Mouse)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    WT with deletion of the 441 N-terminal residues.

    Promoter

    Chicken Beta actin

    Available since

    Oct. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. BII-gR-BtW-eSpCas9

    Plasmid

    #133358

    Purpose

    Piggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications). Provides gRNA and EspCas9 expression

    Depositor

    Blair Madison

    Article

    Fischer et al Am J Physiol Gastrointest Liver Physiol.

    Insert

    eSpCas9
    

    Use

    Tags

    Flag-tagged eSpCas9

    Expression

    Mammalian

    Mutation

    eSpCas9 mutations

    Promoter

    CMV enhancer and hEf1a (CpG free)

    Available since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLPC-MYC.Mm.KAT5

    Plasmid

    #184652

    Purpose

    Retroviral expression of mouse Kat5

    Depositor

    Agnel Sfeir

    Article

    Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

    Insert

    Kat5 (Kat5 Mouse)
    

    Use

    Retroviral

    Tags

    MYC

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLCKO_PSMD1_sgRNA_5

    Plasmid

    #74181

    Purpose

    lentiviral vector expressing sgRNA targeting PSMD1

    Depositor

    Jason Moffat

    Article

    Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10

    Insert

    PSMD1 sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 Promoter

    Available since

    March 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28a-baPrs-hdNadV

    Plasmid

    #91950

    Purpose

    pET28a-baPrs-hdNadV is a bicistronic vector designed for the simultaneous expression of PRS from Bacillus amyloliquefaciens and NadV from Haemophilus ducreyi

    Depositor

    George Cătălin Marinescu

    Article

    Marinescu et al Sci Rep. 2018 Aug 16;8(1):12278. doi: 10

    Inserts

    Putative Nicotinamide Phosphoribosyl Transferase (nadV Haemophilus ducreyi, strain: ATCC 27722)
    Phosphoribosyl Pyrophosphate Synthetases
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changed Leucine 135 to Isoleucine

    Promoter

    T7

    Available since

    Sept. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLCKO_PSMD1_sgRNA_1

    Plasmid

    #74180

    Purpose

    lentiviral vector expressing sgRNA targeting PSMD1

    Depositor

    Jason Moffat

    Article

    Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10

    Insert

    PSMD1 sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 Promoter

    Available since

    March 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBa.GFP-KIF3B tail 391-747

    Plasmid

    #134615

    Purpose

    Mammalian expression of mouse KIF3B 391-747

    Depositor

    Marvin Bentley

    Article

    Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

    Insert

    KIF3B (Kif5b Mouse)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    WT with deletion of the 390 N-terminal residues.

    Promoter

    Chicken Beta actin

    Available since

    April 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBa.FRB-3myc-GS-KIF3A tail 419-702

    Plasmid

    #134614

    Purpose

    Mammalian expression of human KIF3A 419-702

    Depositor

    Marvin Bentley

    Article

    Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

    Insert

    KIF3A (KIF3A Human)
    

    Use

    Tags

    FRB, 3myc

    Expression

    Mammalian

    Mutation

    WT with deletion of the 418 N-terminal residues.

    Promoter

    Chicken Beta actin

    Available since

    Jan. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PV225

    Plasmid

    #132334

    Purpose

    dCas9 plant transcriptional activator (AT-CBF1) linked constitutive expression cassette for Zea mays

    Depositor

    Joshua Young

    Article

    Young et al Commun Biol. 2019 Oct 18;2:383. doi: 10.

    Insert

    dCas9-AT-CBF1 (CBF1 Cas9 (Streptococcus pyogenes); AT-CBF1 (Arabidopsis thaliana))
    

    Use

    Tags

    AT-CBF1

    Expression

    Plant

    Mutation

    Codon optimize for expression and stability in Ze…

    Promoter

    Available since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEGFP-N1-WDR34

    Plasmid

    #66797

    Purpose

    Expression of human WDR34 tagged with GFP under the control of CMV

    Depositor

    Lotte Pedersen

    Article

    Goggolidou et al Development. 2014 Oct;141(20):3966-77. d

    Insert

    WDR34 (WDR34 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 15, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pCW57.1-pDUXChd:hDUX4ctd-ca

    Plasmid

    #198237

    Purpose

    Lentivector with tet-inducible porcine DUXC/human DUX4 chimera (codon altered)

    Depositor

    Stephen Tapscott

    Article

    Nip et al Hum Mol Genet. 2023 Feb 2:ddad021. doi:

    Insert

    DUX4L pDUXChd:hDUX4ctd (codon altered) (DUX4 S. scrofa (porcine), Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    codon altered

    Promoter

    TRE

    Available since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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