We narrowed to 6,650 results for: tac

1. pAJM.715

   Plasmid

   #108516

   Purpose

   PTac-YFP Reporter. IPTG sensor for use in Marionette strain.

   Depositor

   Christopher Voigt

   Article

   Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

   Insert

   PTac-YFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC_sgRNA

   Plasmid

   #68710

   Purpose

   Contains the sgRNA backbone sequence (tracrRNA, 82 bp) and is used as DNA template to amplify a specific sgRNA using a forward primer with the protospacer sequence for gene targeting.

   Depositor

   Roberto Docampo

   Article

   Lander et al MBio. 2015 Jul 21;6(4). pii: e01012-15.

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. SmoCFP

   Plasmid

   #128156

   Purpose

   Expression of Smoothened

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Ott et al Cilia. 2012 Apr 25;1(1):3. doi: 10.1186/

   Insert

   Smoothened (Smo Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJN105-Hygro-GFP

   Plasmid

   #41883

   Depositor

   Howard Steinman

   Article

   Bandyopadhyay et al Infect Immun. 2004 Aug;72(8):4541-51.

   Insert

   Ptac::GFP-mut-2
   

   Use

   Bacterial broad host range vector

   Tags

   Expression

   Bacterial

   Mutation

   GFP-mut-2 is GFP with Ser65Ala, Val68Leu, and Ser…

   Promoter

   Ptac

   Available since

   June 11, 2013

   Availability

   Academic Institutions and Nonprofits only

5. VV020: WT Arch-3 - eGFP in pET28b

   Plasmid

   #58488

   Purpose

   Expresses WT Archaerhodopsin-3 fused to eGFP (with 6x C-terminal His tag) in E. coli

   Depositor

   Adam Cohen

   Article

   Maclaurin et al Proc Natl Acad Sci U S A. 2013 Apr 9;110

   Insert

   Archaerhodopsin-3
   

   Use

   Tags

   eGFP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAdEasy-EGFP-Tubulin

   Plasmid

   #24411

   Depositor

   Torsten Wittmann

   Article

   Kumar et al J Cell Biol. 2009 Mar 23. 184(6):895-908

   Insert

   Tubulin (TUBA1B Human)
   

   Use

   Adenoviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 18, 2010

   Availability

   Academic Institutions and Nonprofits only

7. SAFB1-pcDNA3.1

   Plasmid

   #32742

   Depositor

   Steffi Oesterreich

   Article

   Townson et al J Biol Chem. 2003 May 30;278(22):20059-6

   Insert

   SAFB1 (SAFB Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 19, 2013

   Availability

   Academic Institutions and Nonprofits only

8. YIPlac204TKC-E2-Crimson-HDEL

   Plasmid

   #38771

   Depositor

   Benjamin Glick

   Article

   Strack et al Biochemistry. 2009 Sep 8;48(35):8279-81.

   Insert

   E2-Crimson
   

   Use

   Tags

   Linker peptide and yeast ER retention signal sequ…

   Expression

   Yeast

   Mutation

   Promoter

   TPI1

   Available since

   Aug. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. GFP-PICK

   Plasmid

   #31613

   Depositor

   Robert Malenka

   Article

   Citri et al J Neurosci. 2010 Dec 8. 30(49):16437-52.

   Insert

   PICK1 (shRNA insensitive) +shRNA towards PICK1 (Pick1 Rat)
   

   Use

   Lentiviral

   Tags

   GFP, HA, and PICK1 shRNA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 21, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMP118

    Plasmid

    #207947

    Purpose

    GreenBraid destination vector pGBY1 - Eco31I - PaqCI -sfGFP- PaqCI - Eco31I

    Depositor

    Alexis Maizel

    Article

    Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pKD016

    Plasmid

    #207932

    Purpose

    Premade plasma membrane marker, pUBQ10:PiP1;4:mTurquoise2:tHSP18.2M:FastRed, for braiding

    Depositor

    Alexis Maizel

    Article

    Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMito-PAGFP-FRB.

    Plasmid

    #60005

    Purpose

    Expresses MitoTrap tagged with photo-activatable GFP in mammalian cells.

    Depositor

    Stephen Royle

    Article

    Cheeseman et al J Cell Sci. 2013 May 1;126(Pt 9):2102-13

    Type

    Empty backbone

    Use

    Knocksideways

    Tags

    FRB and PAGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pALPS puro miR30-IRF3

    Plasmid

    #101336

    Purpose

    target site ATCAGATCTACAATGAAGGGC

    Depositor

    Jeremy Luban

    Article

    McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

    Insert

    shRNA targeting miR30
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. ATM N-terminal sgRNA

    Plasmid

    #207089

    Purpose

    pX330 based plasmid for expression of Cas9 and the ATCATTAAGTACTAGACTCA sgRNA to target the ATM locus.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    ATCATTAAGTACTAGACTCA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    April 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMP119

    Plasmid

    #207949

    Purpose

    GreenBraid destination vector pGBY2 - Eco31I - PaqCI -sfGFP- PaqCI - Eco31I

    Depositor

    Alexis Maizel

    Article

    Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pKE014

    Plasmid

    #207908

    Purpose

    GG entry for zCas9i expression

    Depositor

    Alexis Maizel

    Article

    Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

    Insert

    SpCas9_introns_2xNLS-Pea3A(T) term
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCVD438

    Plasmid

    #63759

    Purpose

    eae gene (3476 bp) from pJY21 removed by SamI/SphI digestion, cloned into EcoRV/SphI sites of pACYC184. Insertional inactivation of tetracycline gene.

    Depositor

    James Kaper

    Article

    Jerse et al Proc Natl Acad Sci U S A. 1990 Oct;87(20

    Insert

    eae
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 6, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pMP107

    Plasmid

    #207925

    Purpose

    Premade plasma membrane marker, pUBQ10:PiP1;4:PmScarlet-I:tHSP18.2M, for braiding

    Depositor

    Alexis Maizel

    Article

    Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLH-stsgRNA2.1

    Plasmid

    #64117

    Purpose

    Vector for expression of St1 sgRNA2.1 in mammalian cells

    Depositor

    Thoru Pederson

    Article

    Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

    Insert

    St1 sgRNA2.1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAM750

    Plasmid

    #207889

    Purpose

    Level 0 for A3 module (5' TCCC-3' AACA)

    Depositor

    Alexis Maizel

    Article

    Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

    Insert

    dummy 20nt sequence
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge