We narrowed to 4,342 results for: Pet2;

1. pET28b-6His-AcpS

   Plasmid

   #145376

   Purpose

   Expression of E. coli AcpS

   Depositor

   Robert Tomko

   Article

   Nemec et al Yeast. 2020 May 13. doi: 10.1002/yea.347

   Insert

   AcpS (acpP E. coli)
   

   Tags

   6-His

   Expression

   Bacterial

   Available Since

   May 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pAR127_StrepI_m(KS_MAT)_mouseL_ACP_H8_pET22b

   Plasmid

   #122850

   Purpose

   Expresses a designed construct of the condensing part fused to the ACP domain of murine type I fatty acid synthase (FASN) in Escherichia coli

   Depositor

   Martin Grininger

   Article

   Rittner et al Protein Sci. 2019 Feb;28(2):414-428. doi

   Insert

   m(KS_MAT)_mouseL_ACP (Fasn Mouse)
   

   Tags

   His8 tag and Twin-Strep tag

   Expression

   Bacterial

   Mutation

   wildtype

   Promoter

   T7 promoter

   Available Since

   March 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET21-pelB-LaM-8

   Plasmid

   #172772

   Purpose

   Bacterial expression of anti-mCherry nanobody LaM-8, with pelB leader and C-term free cysteine and 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaM-8
   

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET21a-C2m2-mKate

   Plasmid

   #208765

   Purpose

   To express C2m2-mKate in bacteria

   Depositor

   Urte Neniskyte

   Article

   Dirvelyte et al Cell Mol Biol Lett. 2023 Jul 27;28(1):59

   Insert

   C2m2-mKate (Mfge8 Mouse)
   

   Tags

   mKate

   Expression

   Bacterial

   Mutation

   Mutated 24 and 45 lysine to asparagine in C2 doma…

   Promoter

   T7 promoter

   Available Since

   Oct. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET24-ISWI-iCBD

   Plasmid

   #105499

   Purpose

   Expression of Drosophila ISWI C-terminally tagged with Combined Sce VMA intein and chitin binding domain (iCBD)

   Depositor

   James Kadonaga

   Article

   Khuong et al J Biol Chem. 2017 Nov 24;292(47):19478-1

   Insert

   ISWI-iCBD
   

   Available Since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a(+)::MBP-NL3F10H

   Plasmid

   #141291

   Purpose

   MBP:NLUC:3xFlag:10xHis in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)

   Depositor

   Andrew Millar

   Article

   Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10

   Insert

   MBP-NLUC3F10H
   

   Use

   Luciferase

   Tags

   Flag (x3), His (x10), and MBP

   Expression

   Bacterial

   Mutation

   Engineered for high stability (t1/2 = 11.5 days a…

   Promoter

   promoter for bacteriophage T7 RNA polymerase

   Available Since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28-SpyCatcher002-MBP

   Plasmid

   #216295

   Purpose

   Expresses SpyCatcher002 linked to maltose-binding protein in bacterial cells

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   SpyCatcher002-MBP
   

   Tags

   His6 tag and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28a-His-MinD_MinE

   Plasmid

   #133621

   Purpose

   Expression vector for the Escherichia coli MinD with an N-terminal His-tag for purification. MinE is encoded in the ORF to avoid toxicity when overexpressing MinD, but MinE is not tagged.

   Depositor

   Petra Schwille

   Article

   Loose et al Science. 2008 May 9;320(5877):789-92. do

   Insert

   His-MinD_MinE (minD, minE E. coli)
   

   Expression

   Bacterial

   Available Since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET21-pelB-LaM-2

   Plasmid

   #172768

   Purpose

   Bacterial expression of anti-mCherry nanobody LaM-2, with pelB leader and C-term free cysteine and 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaM-2
   

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28a-HaloTag7SS-DogTag

    Plasmid

    #171775

    Purpose

    Expresses in bacterial cytoplasm HaloTag7 with DogTag inserted between D139 and E140, as well as C61S and C261S in HaloTag7 to block disulfide bond formation

    Depositor

    Mark Howarth

    Article

    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

    Insert

    HaloTag7SS-DogTag
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    DogTag inserted in HaloTag7 between residues D139…

    Promoter

    T7

    Available Since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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