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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 15,942 results for: LEA

Showing: 5301 - 5350 of 15942 results

  2. pToxEXPr6

    Plasmid
    #218290
    Purpose
    Introduction of a cyanamide-inducible I-SceI expression cassette and a TetR-TUP1 expression cassette, use together with pJQR3
    Depositor
    Claudia Vickers
    Article
    McDonnell et al bioRxiv 2024.03.26.586389
    Insert
    hph>tCYC1-ISceI.site-Repeat2-pDDI2>ISceI>tSynth3-pHAC1>TetR>TUP1>tABF1-HO(182,1979)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available Since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    218290-plasmid-map-sequence-id-433735

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  3. pJQR3

    Plasmid
    #218289
    Purpose
    Introduction of a Zif268-hER-VP16 expression cassette and a LmrA-Mig1C expression cassette, use together with pToxEXPR6
    Depositor
    Claudia Vickers
    Article
    McDonnell et al bioRxiv 2024.03.26.586389
    Insert
    HO(-253,-1)-pADH1>LmrA>MIG1(aa123-504)>tMIG1-tYGL036W
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available Since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    218289-plasmid-map-sequence-id-433691

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  4. pAAV/CMV-SCLM

    Plasmid
    #216758
    Purpose
    AAV2 transfer vector with the CMV promoter for ubiquitous expression of SuperClomeleon
    Depositor
    George Augustine
    Insert
    SuperClomeleon followed by WPRE and human growth hormone polyA terminator
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    S30R and 11 AA deletion in the Cerulean CFP moiet…
    Promoter
    CMV (cytomegalovirus)
    Available Since
    April 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    216758-plasmid-map-sequence-id-429489

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  6. pMAT_M1_YLVV

    Plasmid
    #197081
    Purpose
    This plasmid contains the coding sequence for signaling peptide M1, which contains the YLVV signaling motif. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFP
    Depositor
    Wendell Lim
    Article
    Daniels et al Science. 2022 Dec 16;378(6625):1194-1200
    Insert
    This plasmid contains the coding sequence for signaling peptide M1, which contains the YLVV signaling motif.
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    April 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    197081-plasmid-map-sequence-id-386808

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  7. pMAT_M2_YVPM

    Plasmid
    #197082
    Purpose
    This plasmid contains the coding sequence for signaling peptide M2, which contains the YVPM signaling motif. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFP
    Depositor
    Wendell Lim
    Article
    Daniels et al Science. 2022 Dec 16;378(6625):1194-1200
    Insert
    This plasmid contains the coding sequence for signaling peptide M2, which contains the YVPM signaling motif.
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    April 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    197082-plasmid-map-sequence-id-386820

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  8. pMAT_M3_YENL

    Plasmid
    #197083
    Purpose
    This plasmid contains the coding sequence for signaling peptide M3, which contains the YENL signaling motif. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFP
    Depositor
    Wendell Lim
    Article
    Daniels et al Science. 2022 Dec 16;378(6625):1194-1200
    Insert
    This plasmid contains the coding sequence for signaling peptide M3, which contains the YENL signaling motif.
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    April 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    197083-plasmid-map-sequence-id-386822

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  9. pMAT_M5_YESP

    Plasmid
    #197085
    Purpose
    This plasmid contains the coding sequence for signaling peptide M5, which contains the YESP signaling motif. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFP
    Depositor
    Wendell Lim
    Article
    Daniels et al Science. 2022 Dec 16;378(6625):1194-1200
    Insert
    This plasmid contains the coding sequence for signaling peptide M5, which contains the YESP signaling motif.
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    April 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    197085-plasmid-map-sequence-id-387437

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  10. pMAT_M6_ITYAAV

    Plasmid
    #197086
    Purpose
    This plasmid contains the coding sequence for signaling peptide M6, which contains the ITYAAV signaling motif. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFP
    Depositor
    Wendell Lim
    Article
    Daniels et al Science. 2022 Dec 16;378(6625):1194-1200
    Insert
    This plasmid contains the coding sequence for signaling peptide M6, which contains the ITYAAV signaling motif.
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    April 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    197086-plasmid-map-sequence-id-392959

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  11. pMAT_M7_PVQP

    Plasmid
    #197087
    Purpose
    This plasmid contains the coding sequence for signaling peptide M7, which contains the PVQP signaling motif. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFP
    Depositor
    Wendell Lim
    Article
    Daniels et al Science. 2022 Dec 16;378(6625):1194-1200
    Insert
    This plasmid contains the coding sequence for signaling peptide M7, which contains the PVQP signaling motif.
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    April 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    197087-plasmid-map-sequence-id-386800

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  12. pMAT_M8_RSTKP

    Plasmid
    #197088
    Purpose
    This plasmid contains the coding sequence for signaling peptide M8, which contains the RSTKP signaling motif. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFP
    Depositor
    Wendell Lim
    Article
    Daniels et al Science. 2022 Dec 16;378(6625):1194-1200
    Insert
    This plasmid contains the coding sequence for signaling peptide M8, which contains the RSTKP signaling motif.
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    April 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    197088-plasmid-map-sequence-id-386823

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  13. pMAT_M9_PVQE

    Plasmid
    #197091
    Purpose
    This plasmid contains the coding sequence for signaling peptide M9, which contains the PVQE signaling motif. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFP
    Depositor
    Wendell Lim
    Article
    Daniels et al Science. 2022 Dec 16;378(6625):1194-1200
    Insert
    This plasmid contains the coding sequence for signaling peptide M9, which contains the PVQE signaling motif.
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    April 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    197091-plasmid-map-sequence-id-387438

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  22. pETM11-6xHis-TEV-hORF1p_StammerAAA

    Plasmid
    #202587
    Purpose
    Allows for IPTG-inducible expression of the human L1RP ORF1 protein with the M91A, E92A, and L93A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification
    Depositor
    Liam Holt
    Article
    Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75
    Insert
    ORF1p
    Use
    Tags
    6x His and TEV protease cleavage site
    Expression
    Bacterial
    Mutation
    Changed ORF1p Methionine 91 to Alanine, ORF1p Glu…
    Promoter
    Available Since
    Nov. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    202587-plasmid-map-sequence-id-415633

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  24. MLKP2delKASH

    Plasmid
    #130838
    Purpose
    Expression of plant LINC fluorescent fusion protein for cell biology
    Depositor
    Hank Bass
    Article
    Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs
    Insert
    MLKP2
    Use
    Tags
    eGFP-FLAG-HA
    Expression
    Plant
    Mutation
    Deletion of KASH domain, addition of three alani…
    Promoter
    CaMV 35S
    Available Since
    Sept. 28, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    130838-plasmid-map-sequence-id-255274

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  26. pCAGGS-C-TEV-HAT

    Plasmid
    #202597
    Purpose
    Express N-terminal Tobacco Etch Virus (TEV) protease cleavable Histidine Affinity Tag (HAT) (KDHLIHNVHKEEHAHAHNK) in mammalian cell
    Depositor
    Chiaki Murakami
    Article
    Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002
    Type
    Empty backbone
    Use
    Tags
    TEV cleavable site (ENLYFQG) and poly-histidine a…
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available Since
    July 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    202597-plasmid-map-sequence-id-399552

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  27. pCAGGS-N-TEV-HAT

    Plasmid
    #202598
    Purpose
    Express C-terminal Tobacco Etch Virus (TEV) protease cleavable Histidine Affinity Tag (HAT) (KDHLIHNVHKEEHAHAHNK) in mammalian cell
    Depositor
    Chiaki Murakami
    Article
    Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002
    Type
    Empty backbone
    Use
    Tags
    TEV cleavable site (ENLYFQG) and poly-histidine a…
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available Since
    July 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    202598-plasmid-map-sequence-id-399468

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  28. pOET3-C-TEV-Twin-Strep

    Plasmid
    #203324
    Purpose
    Generate the baculovirus to express N-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK) in insect cells
    Depositor
    Chiaki Murakami
    Article
    Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002
    Type
    Empty backbone
    Use
    Tags
    TEV cleavable site (ENLYFQG) and Twin-strep-tag
    Expression
    Insect
    Mutation
    Promoter
    Available Since
    July 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    203324-plasmid-map-sequence-id-399463

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Showing: 5301 - 5350 of 15942 results